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CFX96 Touch Deep Well Real-Time PCR Detection System

CFX96 Touch Deep Well Real-Time PCR Detection System

The CFX96 Touch Deep Well Real-Time PCR System offers industry-leading technology for large-volume reactions in real-time PCR applications.

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Overview

CFX System

Try out a CFX Real-Time PCR System in your laboratory.

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CFX System

Real-Time PCR Tips, Tricks, and Best Practices Guide

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The CFX96 Touch Deep Well Real-Time PCR Detection System offers precise quantification and target discrimination for up to 5 targets in large-volume reactions. The system incorporates industry-leading technology and stand-alone functionality to provide a robust and easy-to-use qPCR instrument for reliable results.

Key Features and Benefits

The CFX96 Touch Deep Well Real-Time PCR System provides:

  • Minimal setup with factory-calibrated dyes
  • Easy viewing of real-time data traces with integrated LCD touch screen
  • Fast experiment optimization with built-in thermal gradient
  • User-friendly data analysis modules with CFX Maestro Software
  • Effortless export of RDML files for import into qbasePLUS Software

Related Technologies

Related Products

Specifications

Thermal Cycler
 
Chassis
C1000 Touch™
Maximum ramp rate, °C/sec
2.5
Average ramp rate, °C/sec
2
Heating and cooling method
Peltier
Lid, °C
Heats up to 105
Temperature
 
Range, °C
0–100
Accuracy, °C
±0.2 of programmed target at 90°C
Uniformity, °C
±0.4 well-to-well within 10 sec of arrival at 90°C
Gradient
 
Operational range, °C
30–100
Programmable span, °C
1–24
Optical Detection
 
Excitation
6 filtered LEDs
Detection
6 filtered photodiodes
Range of excitation/emission wavelengths, nm
450–730
Sensitivity
Detects 1 copy of target sequence in human genomic DNA
Dynamic range
10 orders of magnitude
Scan Time
 
All channels, sec
12
FAM/SYBR® Green only, sec
3
Software
 
Operating systems
Windows XP, Windows 7, Windows 10
Multiplex analysis
Up to 5 targets per well
System
 
Licensed for real-time PCR
Yes
Sample capacity, wells
96
Sample size, µl
10–125
Communication interface
USB 2.0
Electrical approvals
IEC, CE
Dimensions (W x D x H), cm/in
33 x 46 x 36/13 x 18 x 14
Weight, kg/lb
21/47

Ordering

items

Use the filters below to refine results!

(Discontinued) CFX96 Deep Well Optical Reaction Module for Real-Time PCR Systems
1844095
96–deep well optical module for real-time PCR, includes cables, for use with C1000 Touch Thermal Cycler Chassis

Accessories

(Discontinued) CFX Qualification Plate
1845098
Predispensed 96-well plate for performance validation of CFX96™, CFX96 Touch™, CFX96 Touch™ Deep Well, or CFX Connect™ Real-Time PCR Detection System, includes template DNA, primers, supermix, water

Kits & Reagents

(Discontinued) SsoAdvanced Universal SYBR® Green Supermix, 200 x 20 µl rxns, 2 ml (2 x 1 ml)
1725270
2 ml (2 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX normalization dyes, for 200 x 20 µl reactions
(Discontinued) SsoAdvanced™ Universal SYBR® Green Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)
1725271
5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX Normalization Dyes, for 500 x 20 µl reactions
(Discontinued) SsoAdvanced Universal SYBR® Green Supermix, 1,000 x 20 µl rxns, 10 ml (10 x 1 ml)
1725272
10 ml (10 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX normalization dyes, for 1,000 x 20 µl reactions
(Discontinued) SsoAdvanced Universal SYBR® Green Supermix, 2,500 x 20 µl rxns, 25 ml (5 x 5 ml)
1725274
25 ml (5 x 5 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX normalization dyes, for 2,500 x 20 µl reactions
(Discontinued) SsoAdvanced Universal SYBR® Green Supermix, 5,000 x 20 µl rxns, 50 ml (10 x 5 ml)
1725275
50 ml (10 x 5 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX normalization dyes, for 5,000 x 20 µl reactions
(Discontinued) SsoAdvanced Universal Probes Supermix, 200 x 20 µl rxns, 2 ml (2 x 1 ml)
1725280
2 ml (2 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, stabilizers, ROX normalization dyes, for 200 x 20 µl reactions
(Discontinued) SsoAdvanced Universal Probes Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)
1725281
5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, stabilizers, ROX Normalization Dyes, for 500 x 20 µl reactions
(Discontinued) SsoAdvanced Universal Probes Supermix, 1,000 x 20 µl rxns, 10 ml (10 x 1 ml)
1725282

10 ml (10 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, stabilizers, ROX normalization dyes, for 1,000 x 20 µl reactions

(Discontinued) SsoAdvanced Universal Probes Supermix, 2,500 x 20 µl rxns, 25 ml (5 x 5 ml)
1725284

25 ml (5 x 5 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, stabilizers, ROX normalization dyes, for 2,500 x 20 µl reactions

(Discontinued) SsoAdvanced Universal Probes Supermix, 5,000 x 20 µl rxns, 50 ml (10 x 5 ml)
1725285

50 ml (10 x 5 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, stabilizers, ROX normalization dyes, for 5,000 x 20 µl reactions

(Discontinued) iTaq™ Universal SYBR® Green Supermix, 200 x 20 µl rxns, 2 ml (2 x 1 ml)
1725120

200 x 20 µl reactions, 2 ml (2 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, and a blend of passive reference dyes

(Discontinued) iTaq™ Universal SYBR® Green Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)
1725121

500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, and a blend of passive reference dyes

(Discontinued) iTaq™ Universal SYBR® Green Supermix, 1,000 x 20 µl rxns, 10 ml (10 x 1 ml)
1725122

1,000 x 20 µl reactions, 10 ml (10 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, and a blend of passive reference dyes

(Discontinued) iTaq™ Universal SYBR® Green Supermix, 2,500 x 20 µl rxns, 25 ml (5 x 5 ml)
1725124

2,500 x 20 µl reactions, 25 ml (5 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, and a blend of passive reference dyes

(Discontinued) iTaq™ Universal SYBR® Green Supermix, 5,000 x 20 µl rxns, 50 ml (10 x 5 ml)
1725125

5,000 x 20 µl reactions, 50 ml (10 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, and a blend of passive reference dyes

(Discontinued) iTaq™ Universal Probes Supermix, 200 x 20 µl rxns, 2 ml (2 x 1 ml)
1725130
200 x 20 µl reactions, 2 ml (2 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA polymerase, MgCl2, enhancers, stabilizers, and ROX normalization dyes
(Discontinued) iTaq™ Universal Probes Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)
1725131
500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, enhancers, stabilizers, and ROX Normalization Dyes
(Discontinued) iTaq™ Universal Probes Supermix, 1,000 x 20 µl rxns, 10 ml (10 x 1 ml)
1725132
1,000 x 20 µl reactions, 10 ml (10 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA polymerase, MgCl2, enhancers, stabilizers, and ROX normalization dyes
(Discontinued) iTaq™ Universal Probes Supermix, 2,500 x 20 µl rxns, 25 ml (5 x 5 ml)
1725134
2,500 x 20 µl reactions, 25 ml (5 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq DNA polymerase, MgCl2, enhancers, stabilizers, and ROX normalization dyes
(Discontinued) iTaq™ Universal Probes Supermix, 5,000 x 20 µl rxns, 50 ml (10 x 5 ml)
1725135
5,000 x 20 µl reactions, 50 ml (10 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq DNA polymerase, MgCl2, enhancers, stabilizers, and ROX normalization dyes
(Discontinued) iTaq™ Universal SYBR® Green One-Step Kit, 100 x 20 µl rxns, 1 ml
1725150
100 x 20 µl reactions, 1 ml (1 x 1 ml), one-step RT-PCR kit, includes 2x one-step SYBR® Green reaction mix containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript™ reverse transcriptase; and nuclease-free water
(Discontinued) iTaq™ Universal SYBR® Green One-Step Kit, 500 x 20 µl rxns, 5 ml (5 x 1 ml)
1725151
500 x 20 µl reactions, 5 ml (5 x 1 ml), one-step RT-PCR kit, includes 2x one-step SYBR® Green reaction mix containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript™ reverse transcriptase; and nuclease-free water
(Discontinued) iTaq Universal Probes One-Step Kit, 100 x 20 µl rxns, 1 ml
1725140
100 x 20 µl reactions, 1 ml (1 x 1 ml) 2x one-step reaction mix, containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript reverse transcriptase; and nuclease-free water
(Discontinued) iTaq Universal Probes One-Step Kit, 500 x 20 µl rxns, 5 ml (5 x 1 ml)
1725141
500 x 20 µl reactions, 5 ml (5 x 1 ml) 2x one-step reaction mix containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript reverse transcriptase; and nuclease-free water
(Discontinued) Precision Melt Supermix for High Resolution Melt (HRM) Analysis, 200 x 20 µl reactions, 2 x 1 ml
1725110
2 x 1 ml, 2x real-time PCR mix, contains dNTPs, iTaq™ DNA polymerase, MgCl2, EvaGreen dye, stabilizers, for 200 x 20 µl reactions
(Discontinued) Precision Melt Supermix for High Resolution Melt (HRM) Analysis, 1,000 x 20 µl reactions, 10 x 1 ml
1725112
10 x 1 ml, 2x real-time PCR mix, contains dNTPs, iTaq™ DNA polymerase, MgCl2, EvaGreen dye, stabilizers, for 1,000 x 20 µl reactions
(Discontinued) iQ™ Multiplex Powermix, 50 x 50 µl rxns, 1.25 ml
1725848
50 x 50 µl reactions, real-time PCR mix for detection of multiple targets in a single tube, 2x mix contains dNTPs, 11 mM MgCl2, iTaq™ DNA polymerase
(Discontinued) iQ™ Multiplex Powermix, 200 x 50 µl rxns, 5 ml (4 x 1.25 ml)
1725849
200 x 50 µl reactions, real-time PCR mix for detection of multiple targets in a single tube, 2x mix contains dNTPs, 11 mM MgCl2, iTaq™ DNA polymerase
(Discontinued) iScript™ Select cDNA Synthesis Kit, 25 x 20 µl rxns
1708896
25 x 20 µl reactions, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water
(Discontinued) iScript™ Select cDNA Synthesis Kit, 100 x 20 µl rxns
1708897
100 x 20 µl reactions, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water
(Discontinued) iScript™ Select cDNA Synthesis Kit, 500 x 20 µl rxns
1708897BUN
500 x 20 µl reactions, includes 5x reaction mix, iScript Reverse Transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water
(Discontinued) iScript™ Reverse Transcription Supermix, 25 x 20 µl rxns, 100 µl
1708840
25 x 20 µl reactions, 100 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 50 reactions of no-RT control supermix

Plastics

(Discontinued) Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, white/clear
HSP9601

Pkg of 50, white shell/clear well PCR plate, rigid 2-component design

(Discontinued) Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, white/clear, barcoded
HSP9901

Pkg of 50, white shell/clear well PCR plate, barcoded, 7-digit sequential, Code 128, rigid 2-component design

(Discontinued) Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, white/white
HSP9655
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, rigid 2-component design
(Discontinued) Multiplate™ 96-Well PCR Plates, low profile, unskirted, clear
MLL9601
Pkg of 25, clear PCR plate, one-component polypropylene, can be cut for fewer samples
(Discontinued) Multiplate™ 96-Well PCR Plates, low profile, unskirted, white
MLL9651
Pkg of 25, white PCR plate, one-component polypropylene, can be cut for fewer samples
(Discontinued) 0.2 ml 8-Tube PCR Strips without Caps, low profile, clear
TLS0801

Pkg of 120, thin-wall polypropylene 8-tube strips for PCR and low-volume reactions, clear (960 tubes)

(Discontinued) 0.2 ml 8-Tube PCR Strips without Caps, low profile, white
TLS0851

Pkg of 120, thin-wall polypropylene 8-tube strips for PCR and low-volume reactions, white (960 tubes)

TCS0803 TN
(Discontinued) 0.2 ml Flat PCR Tube 8-Cap Strips, optical, ultraclear
TCS0803

Pkg of 120, 8-cap strips for 0.2 ml tubes and plates for PCR and optical reactions, ultraclear

Downloads

  • Precision Melt Analysis™ v1.2 Software Upgrade
    SOFT-PM-12-PATCH
    Upgrade Precision Melt Analysis Software from version 1.0 or 1.1 to version 1.2 for use with CFX Manager™ Software version 2.0 and higher
  • CFX Manager™ Software v3.0 Upgrade
    SOFT-CFX-30-PATCH
    Upgrade CFX Manager software from version 1.0–2.1 to version 3.0 for all CFX and MiniOpticon real-time PCR detection systems. If using CFX Manager software version 2.0 or lower, a firmware update is required for all CFX systems

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