SsoAdvanced Universal SYBR® Green Supermix, 200 x 20 µl rxns, 2 ml (2 x 1 ml) #1725270



2 ml (2 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX normalization dyes, for 200 x 20 µl reactions
Safety Data Sheet

Safety Data Sheet



SsoAdvanced Universal SYBR Green Supermix is a high-performance real-time PCR supermix based on Bio-Rad's patented* Sso7d fusion protein technology. This supermix is formulated for a wide range of real-time PCR applications and for use with all ROX dependent or independent real-time PCR systems. The dsDNA binding protein, Sso7d, stabilizes the polymerase-template complex, providing superior inhibitor tolerance, increased processivity, specificity, and greater speed without affecting PCR sensitivity, efficiency, or reproducibility.

SsoAdvanced Universal SYBR Green Supermix lets you:

  • Use any real-time PCR system — the universal reference dye in this supermix enables ROX normalization of qPCR data regardless of the ROX level requirements of the qPCR system
  • Achieve superior real-time PCR results under various conditions — our robust formulation delivers consistent performance in fast cycling across a broad range of reaction conditions, primer concentrations, and temperature ranges
  • Increase your qPCR sensitivity and efficiency of detection from compromised samples — the Sso7d fusion polymerase has increased resistance to a wide variety of PCR inhibitors, which helps provide better sensitivity and overall performance
  • Decrease time to results without compromising qPCR data quality — the Sso7d fusion polymerase and optimized buffer together provide rapid polymerization kinetics and instant polymerase activation
  • Obtain better results with Bio-Rad's PrimePCR assays — real-time PCR assays are expertly designed and wet-lab validated to ensure optimal assay performance

Instrument Compatibility

SsoAdvanced Universal SYBR Green Supermix is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:

Applied Biosystems
  • StepOne/StepOnePlus
  • 7500, ViiA 7
  • 7000, 7300, 7700, 7900HT, QuantStudio Systems
  • Mx3000P, 3005P, 4000
  • Mastercycler ep realplex 2, 4
  • Corbett Rotor-Gene 3000, 6000, Q
  • LightCycler 480, 96
  • LightCycler 1.0, 1.5, 2.0*
  • LightScanner HR-1
  • LightScanner 32*

* BSA must be added according to instrument specifications

Applications and Uses of SsoAdvanced Universal SYBR Green Supermix

Packaging Options

Number of 20 μl Reactions Total Volume Number of Vials Catalog #
 200 2 ml 2 x 1 ml 1725270
500 5 ml 5 x 1 ml 1725271
1,000 10 ml 10 x 1 ml 1725272
2,500 25 ml 5 x 5 ml 1725274
5,000 50 ml 10 x 5 ml 1725275

Related Products

We have developed SsoAdvanced universal probes supermix, which is available in several formats (200–5,000 reactions).

Bio-Rad offers a complete workflow solution for real-time PCR, including:

More Information

To ensure that your qPCR reactions are reproducible and reliable, follow our step-by-step design and optimization protocols. We also provide insights into analysis of qPCR data and troubleshooting reactions.

* U.S. patent 7,560,260.



Product expiration (after shipping date)
12 months
Storage at 4°C (after thawing)
Up to 3 months
Customer guarantee (minimum usage time) at –20°C
12 months