iTaq™ Universal SYBR® Green One-Step Kit, 500 x 20 µl rxns, 5 ml (5 x 1 ml) #1725151

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Overview

500 x 20 µl reactions, 5 ml (5 x 1 ml), one-step RT-PCR kit, includes 2x one-step SYBR® Green reaction mix containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript™ reverse transcriptase; and nuclease-free water
Safety Data Sheet

Safety Data Sheet

Description

Description

Our Universal Real-Time PCR Reagents Web App brings you in-depth product information, individual and competitive performance data, interactive tutorials, 3-D animated videos, technical manuals, and more.

Use our combined iScript™ RNase H+reverse transcriptase and antibody hot-start iTaq DNA polymerase kit formulation to complete your SYBR® Green real-time reactions in one step — PCR efficiency is improved over a wide dynamic range and under various conditions.

  • Advanced formulation for amplifying difficult targets and challenging samples
  • Can be combined with our PrimePCR™ assays for a simplified workflow
  • Universal — works on any real-time PCR system

Kit Components

  • Reverse transcriptase – 125 µl
  • Reaction mix – 5 ml (5 x 1 ml)

Instrument Compatibility

iTaq Universal SYBR Green One-Step RT-qPCR Kit is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:

Systems
Applied Biosystems
  • StepOne/StepOnePlus
  • 7500, ViiA 7
  • 7000, 7300, 7700, 7900HT, QuantStudio Systems
Stratagene
  • Mx3000P, 3005P, 4000
Eppendorf
  • Mastercycler ep realplex 2, 4
QIAGEN
  • Corbett Rotor-Gene 3000, 6000, Q
Roche
  • LightCycler 480, 96
  • LightCycler 1.0, 1.5, 2.0*
BioFire
  • LightScanner HR-1
  • LightScanner 32*

* BSA must be added according to instrument specifications

Applications and Uses of the iTaq® Universal SYBR® Green One-Step Kit

  • qPCR/real-time PCR
  • Gene expression analysis
  • Absolute quantification
  • Mutation detection
  • Pathogen detection
  • Viral detection (load)
  • Genetically modified organism (GMO) characterization
  • Genetic profiling

Packaging Options

The iTaq™ universal SYBR® Green one-step kit is also available in a format of 100 reactions (171-5150).

Related Products

Bio-Rad has also developed one-step kits for probe-specific applications.

Specifications

Specifications

Product expiration (after shipping date)
12 months
RT storage at –20°C (after shipping date)
12 months
Reaction mix storage at 4°C (after thawing)
Up to 3 months
Customer guarantee (minimum usage time) at –20°C
12 months