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Overview
5,000 x 20 µl reactions, 50 ml (10 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, and a blend of passive reference dyes
Safety Data Sheet
Description
Description
Use iTaq Universal SYBR Green Supermix with blended ROX and fluorescein passive reference dyes for dye-based quantitative PCR (qPCR).
- Ready-to-use mix optimized for dye-based qPCR on any real-time PCR instrument
- Contains both ROX and fluorescein dyes for universal real-time instrument compatibility (ROX-independent and ROX-dependent systems)
- Amplify genomic DNA and difficult amplicons
- Antibody-mediated hot-start iTaq DNA polymerase for rapid activation (30 sec)
Instrument Compatibility
iTaq Universal SYBR Green Supermix is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:
| Systems | |
| Applied Biosystems |
|
| Stratagene |
|
| Eppendorf |
|
| QIAGEN |
|
| Roche |
|
| BioFire |
|
* BSA must be added according to instrument specifications
Packaging Options
| Number of 20 µl Reactions | Supermix Volume, ml | Catalog Number |
| 200 | 2 x 1 | 1725120 |
| 500 | 5 x 1 | 1725121 |
| 1,000 | 10 x 1 | 1725122 |
| 2,500 | 5 x 5 | 1725124 |
| 5,000 | 10 x 5 | 1725125 |
See the iTaq Universal SYBR Green Supermix product page for more information and documentation.
Related Products
Related Categories
- PCR and qPCR Reagents
- Real-Time PCR Detection Systems
- PCR Plastic Consumables
- Instrument Validation Tools and Services
Supporting Documents
- iTaq™ Universal SYBR® Green Supermix Product Insert
- Amplification Plastics and Reagents Selection Guide
