Overview
The QX600 AutoDG Droplet Digital PCR System (ddPCR) offers workflow automation, delivers advanced six-color multiplexing, and absolute quantification of nucleic acid without sacrificing the best-in-class performance you have come to expect from Bio-Rad’s ddPCR systems.
The AutoDG Instrument eliminates user-to-user variability, allowing you to fully exploit the precision of ddPCR technology by providing more consistent droplet counts across users and plates. The AutoDG Instrument also minimizes hands-on time — it takes less than 5 minutes to load the instrument, and no supervision or user activity is required during droplet generation.
Benefits of the QX600 AutoDG Droplet Digital PCR System
The Automated Droplet Generator offers:
- A simplified ddPCR workflow
- Generates droplets for up to 96 ddPCR samples in under 45 minutes
- Compatibility with QX200™ reagents and consumables
- Guided setup and loading with user-friendly touch-screen interface
- Run-to-run flexibility with user-definable plate layout in 8-well increments
- Reduced contamination risk with HEPA-filtered enclosure allowing the system to be placed in any lab environment
The QX600 Droplet Reader offers users:
- Sensitive multiplexing
- Six-color detection capability (FAM, HEX, Cy5, Cy5.5, ROX, and ATTO 590)
- Quantification of up to 12 targets in a single well
- Absolute quantification with 0.1% or better sensitivity
- Sensitive and precise gene expression multiplexing
- Accurate and reproducible results
- Confidence in your results bolstered by a system qualified by standards from National Measurement Institute (NMI), Australia
- Sets the standard for reproducibility as demonstrated in numerous studies
- Powerful software
- User-friendly analysis
- Audit trails and tools for U.S. FDA 21 CFR Part 11 compliance with premium edition
- Assay protocol files for automated data analysis and reporting with premium edition
- Portfolio compatibility
- Cross-compatibility with QX200 reagents, assays, and consumables
- Extensive menu of pre-existing ddPCR assays
- System capable of running both TaqMan® and EvaGreen chemistries
Applications of the QX600 Droplet Digital PCR System
The QX600 System enhances a wide variety of applications, including: oncology research, gene expression, next generation sequencing (NGS) orthogonal testing, cell and gene therapy, food and wastewater testing.
- Oncology research — The QX600 System collects more information per well, preserving precious circulating tumor DNA (ctDNA) and tumor samples.
- Structural variant analysis
- Rare variant detection
- Copy number variation
- Methylation detection
- Loss of heterozygosity
- Gene Expression — Employ the extreme precision of the QX600 System to quantify small-fold changes in low abundance RNA molecules.
- Next generation sequencing (NGS) orthogonal testing — With the QX600 System perform accurate quantification and qualification of NGS libraries without the use of a standard curves.
- Cell and gene therapy — Accelerate therapeutics discovery with highly-advanced multiplexing afforded by the QX600 System.
- Residual DNA detection in biologics
- Genome edit confirmation
- Food testing — Use the QX600 System to perform routine evaluation of genetically modified organisms (GMO) and pathogen contamination detection.
- Wastewater testing — Leverage the QX600 System to perform population-wide monitoring of established and emerging pathogens.
Required Instruments
The QX600 AutoDG Droplet Digital PCR System requires the use of the PTC Tempo Deepwell Thermal Cycler or C1000 Touch Thermal Cycler with 96–Deep Well Reaction Module and the PX1 PCR Plate Sealer.
Required Consumables
ddPCR Reagents for Hydrolysis Probe-Based Applications
- PrimePCR ddPCR Assays
- ddPCR Multiplex Supermix
- ddPCR Supermixes for Probes (No dUTP)
- ddPCR Supermix for Probes
- One-Step RT-ddPCR Advanced Kit for Probes
- Residual DNA Quantification Supermixes
- Automated Droplet Generation Oil for Probes
- Droplet Reader Oil
ddPCR Reagents for EvaGreen Applications
Specifications
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Discover how the QX600 AutoDG Droplet Digital PCR System enables and automates 6-color multiplexing for up to 96-samples per run providing clear discrimination of multiple targets.
The QX600™ Droplet Reader enables advanced six-color multiplexing, allowing clear discrimination of multiple targets with assays that are cross-compatible with the QX200™ Droplet Digital™ PCR (ddPCR™) System. The QX600 Droplet Reader is designed for investigators who need to quantify multiple targets with high accuracy, reproducibility, and sensitivity.
AutoDG Instrument for automated droplet generation of up to 96 samples, for use with the QX600/QX200 Droplet Digital PCR Systems
Accessories
Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, 96–deep well reaction module, USB flash drive
96-well, thermal cycler for PCR with cables; for research use only.
Kits & Reagents
1.2 ml (2 x 0.6 ml), 4x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital PCR Systems
3 ml (5 x 0.6 ml), 4x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital PCR Systems
12.5 ml (5 x 2.5 ml), 4x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital PCR Systems
2 ml (2 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital PCR Systems
5 ml (5 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital PCR Systems
25 ml (5 x 5 ml), 2x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital PCR Systems
2 ml (2 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital™ PCR Systems
5 ml (5 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital™ PCR Systems
25 ml (5 x 5 ml), 2x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital™ PCR Systems
50 ml (10 x 5 ml), 2x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital™ PCR Systems
200 x 20 µl reactions, 2 ml (2 x 1 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX600/QX200 Droplet Digital™ PCR System
500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX600/QX200 Droplet Digital™ PCR System
2,500 x 20 µl reactions, 25 ml (5 x 5 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX600/QX200 Droplet Digital™ PCR System
5,000 x 20 µl reactions, 50 ml (10 x 5 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX600/QX200 Droplet Digital™ PCR System
200 x 20 µl reaction kit, for absolute quantification of target RNA in the QX600/QX200 Droplet Digital™ PCR Systems
500 x 20 µl reaction kit, for absolute quantification of target RNA in the QX600/QX200 Droplet Digital™ PCR Systems
200 x 20 µl reactions, includes ddPCR Library Quantification Assay and ddPCR Supermix for Probes (No dUTP), for quantification of Illumina TruSeq libraries using the QX200™/QX100™ ddPCR™ Systems
Consumables
Pkg of 25, clear well/clear shell semi-skirted plates, for use with QX600/QX200 Droplet Digital™ PCR Systems
Pkg of 30, cartridges for use with the Automated Droplet Generator in the QX600/QX200 AutoDG Droplet Digital PCR System
Pkg of 60, cartridges for use with the Automated Droplet Generator in the QX600/QX200 AutoDG™ Droplet Digital™ PCR System
2 L (2 x 1 L), oil for use with droplet reader in the QX600/QX200 Droplet Digital™ PCR Systems