QX200 AutoDG Droplet Digital PCR System

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QX200 ddPCR System with Automated Droplet Generator

Overview

Digital PCR using QX200™ and AutoDG™ ddPCR Systems
Droplet Digital™ PCR Publications

Browse the growing list of cited peer-reviewed journal articles in Droplet Digital PCR research featuring Bio-Rad products.

The publications appear in journals such as PLOS ONE, Cell, Nucleic Acids Research, and Nature Methods and span the following applications:

Cancer
 
mRNA quantification and expression
Copy number variation
Mutation detection
Droplet Digital PCR methods/ analytical performance
Next-generation sequencing library quantification/validation
Environmental studies
Pathogen detection (microbe/viral)
Epigenetics
Liquid Biopsy Liquid Biopsy
Gene expression  

Browse Publications

The AutoDG Instrument simplifies the Droplet Digital PCR workflow, making digital PCR both scalable and practical. Prepare up to 96 samples at a time with minimal hands-on time and eliminate user-to-user variability.
Read full description

Ordering Information


  • Description
  • Specifications
  • Ordering
  • Accessories
  • Kits & Reagents
  • Consumables
  • Documents

Description

The QX200 AutoDG Droplet Digital PCR System provides absolute quantification of target DNA or RNA molecules for EvaGreen and probe-based Droplet Digital PCR (ddPCR) applications. The Automated Droplet Generator simplifies the ddPCR workflow, making digital PCR both scalable and practical.

The AutoDG Instrument eliminates user-to-user variability, allowing you to fully exploit the precision of ddPCR technology by providing more consistent droplet counts across users and plates. The AutoDG Instrument also minimizes hands-on time — it takes less than 5 minutes to load the instrument, and no supervision or user activity is required during droplet generation.

Features and Benefits of the QX200 AutoDG Droplet Digital PCR System

Automated Droplet Generator — simplifies the ddPCR workflow and offers flexibility:

  • Generates droplets for 96 ddPCR reactions in under 45 minutes
  • Compatible with both the QX100 and QX200 Droplet Digital PCR Systems
  • A single AutoDG Instrument can supply 4–5 Droplet Readers continuously
  • Guides setup and loading with large, color touch-screen interface and LED lighting
  • User-definable plate layout with increments of 8 wells provides run-to-run flexibility
  • HEPA-filtered enclosure reduces the possibility of contamination and allows the system to be placed in any lab environment

Droplet Digital PCR Technology — the most precise and sensitive digital PCR solution for a wide variety of applications, providing:

  • Flexible digital PCR chemistry — optimized for TaqMan Hydrolysis Probe and EvaGreen Assays
  • Nanoliter droplet partitioning — reduces bias from amplification efficiency differences and PCR inhibitors
  • Convenient assay design — standard curves are not required

Applications of the QX200 Droplet Digital PCR System

  • Cancer biomarker studies and copy number variation — measure varying degrees of cancer mutations, detect rare DNA target copies, and resolve copy number variation states with superior sensitivity and resolution.
  • Pathogen detection — employ the extreme precision of the QX200 System to quantify small fold changes in target DNA or RNA molecules in pathogen detection and monitoring
  • Next generation sequencing — perform absolute quantification of an NGS library without the use of a standard curve
  • Gene expression analysis — achieve reliable and reproducible measurements of small fold changes for low-abundance mRNA and miRNA
  • Environmental monitoring — test a wide variety of environmental samples such as soil and water
  • Food testing — perform routine evaluation of genetically modified organisms (GMO) using validated ddPCR methods

Required Instruments

The QX200 AutoDG Droplet Digital PCR System requires the use of the C1000 Touch Thermal Cycler with 96–Deep Well Reaction Module and the PX1 PCR Plate Sealer.

Required Consumables

ddPCR Reagents for EvaGreen or TaqMan Hydrolysis Probe-Based Applications

More Information

Download the Droplet Digital PCR Applications Guide for in-depth information about setting up experiments for applications including:

  • Droplet Digital PCR applications guide
    • Copy number variation analysis
    • Rare mutation and sequence detection
    • Gene expression
    • Next-generation sequencing library analysis

View Performance Data for the QX200 Droplet Digital PCR System.

Watch our Success Stories Videos to learn more about the applications of the Droplet Digital PCR System in diverse research fields.

Browse our Droplet Digital PCR Reference Library with links to scientific journal articles, conference posters, BioRadiations articles, and Bio-Rad technical reports.

Visit our Applications & Technologies pages to learn more about Droplet Digital PCR Technology and the digital PCR workflow.


Specifications

Starting sample size, µl 20
Automated Droplet Generator capacity 1–96 samples/run
Droplets per 20 µl sample 20,000
QX200™ Droplet Reader capacity 1–96 samples
Sample illumination Light-emitting diodes
Sample detection Multi-pixel photon counter
Detection channels FAM (EvaGreen), HEX (VIC)
Linear dynamic range 5 orders of magnitude
Precision ±10%
Droplets per 96-well plate, million ~1.5
Automated Droplet Generator dimensions (W x D x H) Open: 66 x 56 x 89 cm (26 x 22 x 35 in)
Closed: 66 x 56 x 66 cm (26 x 22 x 26 in)
QX200 Droplet Reader dimensions (W x D x H) 66 x 52 x 29 cm (26 x 20 x 11 in)

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QX200 AutoDG Droplet Digital PCR System

1864100
AutoDG Droplet Digital PCR System for EvaGreen or probe-based digital PCR applications, includes Automated Droplet Generator, QX200 Droplet Reader, computer, and QuantaSoft Software

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Automated Droplet Generator

1864101
AutoDG Instrument for automated droplet generation of up to 96 samples, for use with the QX200/QX100; Droplet Digital PCR Systems

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QX200 Droplet Reader

1864003
Droplet reader for EvaGreen or probe-based digital PCR applications, for use with the QX200 Droplet Digital™ System

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Accessories

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PX1 PCR Plate Sealer

1814000
PCR plate sealer, includes heat sealing instrument, plate support block that holds 96-well and 384-well plates, sealing frame, power cord

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$4,305.00 
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C1000 Touch™ Thermal Cycler with 96–Deep Well Reaction Module

1851197
Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, 96–deep well reaction module, USB flash drive

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$8,690.00 
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Kits & Reagents

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ddPCR Multiplex Supermix, 1 ml

12005909
1 ml (2 x 0.5 ml), 4x supermix, for use in sample preparation for droplet generation in the QX200 Droplet Digital PCR Systems

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$328.00 
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ddPCR Multiplex Supermix, 2.5 ml

12005910
2.5 ml (5 x 0.5 ml), 4x supermix, for use in sample preparation for droplet generation in the QX200 Droplet Digital PCR Systems

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$819.00 
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ddPCR Multiplex Supermix, 12.5 ml

12005911
12.5 ml (5 x 2.5 ml), 4x supermix, for use in sample preparation for droplet generation in the QX200 Droplet Digital PCR Systems

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$4,095.00 
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ddPCR™ Supermix for Probes (No dUTP)

1863023
2 ml (2 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX200™/QX100™ Droplet Digital™ PCR Systems

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$218.00 
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ddPCR Supermix for Probes (No dUTP)

1863024
5 ml (5 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX200/QX100 Droplet Digital™ PCR Systems

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$546.00 
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ddPCR Supermix for Probes (No dUTP)

1863025
25 ml (5 x 5 ml), 2x supermix, for use in sample preparation for droplet generation in the QX200/QX100 Droplet Digital PCR Systems

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$2,730.00 
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ddPCR™ Supermix for Probes

1863026
2 ml (2 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX200™/QX100™ Droplet Digital™ PCR Systems

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$218.00 
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ddPCR™ Supermix for Probes

1863010
5 ml (5 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX200™/QX100™ Droplet Digital™ PCR Systems

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$546.00 
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ddPCR™ Supermix for Probes

1863027
25 ml (5 x 5 ml), 2x supermix, for use in sample preparation for droplet generation in the QX200™/QX100™ Droplet Digital™ PCR Systems

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$2,730.00 
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ddPCR™ Supermix for Probes

1863028
50 ml (10 x 5 ml), 2x supermix, for use in sample preparation for droplet generation in the QX200™/QX100™ Droplet Digital™ PCR Systems

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$5,460.00 
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QX200™ ddPCR™ EvaGreen Supermix

1864033
200 x 20 µl reactions, 2 ml (2 x 1 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX200™ Droplet Digital™ PCR System

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$218.00 
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QX200™ ddPCR™ EvaGreen Supermix

1864034
500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX200™ Droplet Digital™ PCR System

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$546.00 
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QX200™ ddPCR™ EvaGreen Supermix

1864035
2,500 x 20 µl reactions, 25 ml (5 x 5 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX200™ Droplet Digital™ PCR System

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$2,730.00 
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QX200™ ddPCR™ EvaGreen Supermix

1864036
5,000 x 20 µl reactions, 50 ml (10 x 5 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX200™ Droplet Digital™ PCR System

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$5,460.00 
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One-Step RT-ddPCR Advanced Kit for Probes

1864021
200 x 20 µl reaction kit, for absolute quantification of target RNA in the QX200™AutoDG™/QX200™/QX100™ Droplet Digital™ PCR Systems

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$1,229.00 
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One-Step RT-ddPCR Advanced Kit for Probes

1864022
500 x 20 µl reaction kit, for absolute quantification of target RNA in the QX200™AutoDG™/QX200™/QX100™ Droplet Digital™ PCR Systems

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$3,071.00 
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QX200™ Buffer Control for EvaGreen

1864052
9 ml (2 x 4.5 ml), 2x buffer, for use as a blank control with Droplet Generation Oil for EvaGreen

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$310.00 
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ddPCR™ Buffer Control for Probes

1863052
9 ml (2 x 4.5 ml), 2x buffer, for use as blank control with Droplet Generation Oil for Probes

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$280.00 
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ddPCR™ Library Quantification Kit for Illumina TruSeq

1863040
200 x 20 µl reactions, includes ddPCR Library Quantification Assay and ddPCR Supermix for Probes (No dUTP), for quantification of Illumina TruSeq libraries using the QX200™/QX100™ ddPCR™ Systems

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$1,066.00 
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Consumables

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If you are placing an order, you may proceed with your order; the account price will be applied if it is lower than the
list price. If the problem persists, please call customer service at or send an
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ddPCR™ 96-Well Plates

12001925
Pkg of 25, clear well/clear shell semi-skirted plates, for use with QX200™/QX100™ or QX200™ AutoDG™ Droplet Digital™ PCR Systems

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$170.00 
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DG32 Automated Droplet Generator Cartridges

1864108
Pkg of 30, cartridges for use with the Automated Droplet Generator in the QX200 AutoDG Droplet Digital PCR System

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$1,829.00 
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DG32™ Automated Droplet Generator Cartridges

1864109
Pkg of 60, cartridges for use with the Automated Droplet Generator in the QX200™ AutoDG™ Droplet Digital™ PCR System

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$3,258.00 
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Automated Droplet Generation Oil for Probes

1864110
140 ml, oil for use with the Automated Droplet Generator for probe-based detection with the QX200 AutoDG Droplet Digital PCR System

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$723.00 
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Automated Droplet Generation Oil for EvaGreen

1864112
140 ml, oil for use with the Automated Droplet Generator for EvaGreen-based detection with the QX200 AutoDG Droplet Digital PCR System

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$723.00 
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Pipet Tips for the AutoDG™ System

1864120
Pkg of 20, boxes of 96 each, pipet tips for use with the Automated Droplet Generator in the QX200™ AutoDG™ Droplet Digital™ PCR System

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$419.00 
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Pipet Tips for the AutoDG™ System

1864121
Pkg of 40, boxes of 96 each, pipet tips for use with the Automated Droplet Generator in the QX200™ AutoDG™ Droplet Digital™ PCR System

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$784.00 
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Pipet Tip Waste Bins for the AutoDG™ System

1864125
Pkg of 10, pipet tip waste bins for use with the Automated Droplet Generator in the QX200™ AutoDG™ Droplet Digital™ PCR System

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$39.00 
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PCR Plate Heat Seal, foil, pierceable

1814040
Pkg of 100, foil seals for PCR and QX200/QX100 Droplet Digital PCR System applications, for use with PX1 PCR Plate Sealer

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$101.00 
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ddPCR™ Droplet Reader Oil

1863004
2 L (2 x 1 L), oil for use with droplet reader in the QX200™/QX100™ Droplet Digital™ PCR Systems

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$1,556.00 
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Documents

Number Description Options
6718 Guidelines for Phasing Variants Using Bio-Rad's Droplet Digital PCR System Application Note Click to download
6561 QX200 AutoDG Droplet Digital PCR System Flier Click to download [ Add to Cart (Free) ]
10043138 Instruction Manual, Automated Droplet Generator for Droplet Digital™ PCR Systems Click to download
10000107223 QX Manager Software Standard Edition, User Guide Click to download
10000107224 QX Manager Software Regulatory Edition, User Guide Click to download
10000118144 QX Developer Software, User Guide Click to download
10028376 QX200™ ddPCR™ EvaGreen Supermix Product Insert Click to download
10048259 ddPCR Supermix for Residual DNA Quantification Product Insert Click to download
6782 ddPCR™ Multiplex Mutation Screening Kits Flier, Ver D Click to download [ Add to Cart (Free) ]
6721 ddPCR™ Supermix for Residual DNA Quantification Flier, Rev A Click to download
6712 Ultra-Sensitive Quantification of Genome Editing Events Using Droplet Digital™ PCR Application Note, Ver B Click to download
7204 Expanded Droplet Digital PCR Multiplexing Capability Using Two Different Strategies, Ver A Click to download
7271 Current Challenges and Future Impact of Cellular and Genetic Immunotherapies for Human Health Click to download

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