PCR and Real-Time PCR Reagents

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Overview

Bio-Rad's reagents are specially formulated to meet both conventional and real-time PCR requirements with minimal need for optimization.

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Description

Description

Bio-Rad's reagents are specially formulated to meet both conventional and real-time PCR requirements with minimal need for optimization. All reagents are qualified for use on Bio-Rad's complete line of thermal cyclers and real-time PCR detection systems, and demonstrate high performance for cDNA, genomic DNA, and plasmid DNA amplification over a wide dynamic range.

iTaq™ DNA Polymerase

iTaq DNA polymerase is an antibody-mediated hot-start DNA polymerase suitable for both conventional and real-time PCR applications that ensures ease of use and high specificity and sensitivity.

2x Master Mix for PCR

Bio-Rad's 2x master mix for PCR is a concentrated solution of Taq DNA polymerase, dNTPs, and all the components required for PCR, except the DNA template and primers. This premixed formulation saves time and reduces contamination by reducing the number of pipetting steps required for PCR setup. The mix is optimized for efficient and reproducible PCR.

The absence of endodeoxyribonucleases, exodeoxyribonucleases, and ribonucleases is confirmed by appropriate quality tests. The master mix is functionally tested in amplification of a single-copy gene from human genomic DNA.

dNTP Mix

This dNTP mix is formulated for optimal performance in real-time PCR applications and is also qualified for use in conventional PCR applications. It provides consistent, high-yield amplification.

SYBR® Green Supermix

iQ™ SYBR Green supermix is formulated for optimal results in real-time PCR based on SYBR Green I detection. It contains SYBR Green I dye, hot-start iTaq DNA polymerase, optimized buffer, and dNTPs qualified for quantitative PCR. Alternately, Bio-Rad's iScript™ one-step RT-PCR kit with SYBR Green allows cDNA synthesis and PCR to be conveniently carried out in the same tube.

Ordering

Ordering

items

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(Discontinued) Chill-Out Liquid Wax
CHO1411EDU
100 ml, vapor barrier for use in thermal cyclers, clear, optical grade; education use only
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1708880EDU
2.5 ml (2 x 1.25 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, for 100 x 50 µl reactions; education use only
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1708870EDU
5 U/μl, 250 U hot-start polymerase for PCR and real-time PCR, includes 1.25 ml each 10x PCR buffer and 50 mM MgCl2 solution; education use only
166-5009EDU_tn.jpg
(Discontinued) Master Mix For PCR
1665009EDU
90 units, 1.2 ml includes 0.075 units per µl Taq DNA polymerase, reaction buffer, 4 mM MgCl2, 0.4 mM of each dNTP (dATP, dCTP, dGTP, dTTP); education use only
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1725310EDU
100 x 50 µl reactions, premixed PCR reagents, includes 2x master mix (0.04 U/µl), DMSO; suitable for most templates; education use only
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1725320EDU
100 x 50 µl reactions, premixed PCR reagents, includes 2x master mix (0.04 U/µl), DMSO; suitable for GC-rich templates; education use only
170-8891EDU_tn.jpg
1708891EDU
100 x 20 µl reactions, reverse-transcription reagent kit, includes 5x iScript reaction mix, iScript reverse transcriptase, nuclease-free water; education use only
170-8890EDU_tn.jpg
1708890EDU
25 x 20 µl reactions, reverse-transcription reagent kit, includes 5x iScript reaction mix, iScript reverse transcriptase, nuclease-free water; education use only
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1725270EDU
2 ml (2 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR Green I, ROX normalization dyes, for 200 x 20 µl reactions; for educational use only

Documents

Documents

Number Description Options
2779
iTaq DNA Polymerase Flier, Rev E
4106269
Instruction Manual, iScript One-Step RT-PCR Kit with SYBR Green, Rev E
5345
Reagents for Reverse Transcription, PCR, and Real-Time PCR Brochure, Rev C
2764
iQ Supermixes for qPCR Flier, Rev E
4106212
Instruction Manual, iQ SYBR Green Supermix, 100 x 50 µl, Rev D
10000083948
Instruction Manual, iProof™ High-Fidelity PCR Master Mix