Description
Description
SsoAdvanced Universal Probes Supermix is a high-performance real-time PCR supermix based on Bio-Rad's patented * Sso7d fusion protein technology. This ordering information is for educational use only. For best ordering options and account pricing in research applications, please see this page: SsoAdvanced Universal Probes Supermix
The supermix is designed to enhance real-time PCR applications across various ROX-dependent or independent systems. It incorporates the dsDNA binding protein Sso7d, which stabilizes the polymerase-template complex. This results in imporved inhibitor tolerance, processivity, and specificity, as well as greater speed, all while maintaining PCR sensitivity, efficiency, and reproducibility.
SsoAdvanced universal probes supermix offers the following:
- High-performance singleplex and duplex reactions — the Sso7d fusion polymerase and its sophisticated composition allow efficient execution of both singleplex and duplex real-time PCR reactions. This not only ensures data accuracy but also offers cost and time efficiency by facilitating the combination of two assays within a single well
- Use any real-time PCR system — the universal reference dye in this supermix enables ROX normalization of quantitative PCR (qPCR) data regardless of the ROX level requirements of the qPCR system
- Compatibility with any real-time PCR system — the supermix contains a universal reference dye that facilitates data normalization using ROX, making it compatible with any real-time PCR system, regardless of specific ROX level requirements
- Reproducible real-time PCR results under various conditions — our formulation provides reliable performance during fast cycling and across diverse reaction conditions, including varying primer concentrations and temperature ranges
- Improved qPCR sensitivity and detection between various samples — the Sso7d fusion polymerase is tolerant of a broad spectrum of PCR inhibitors, which boosts the effectiveness and sensitivity of qPCRDecrease time to results without compromising qPCR data quality — the Sso7d fusion polymerase and optimized buffer together provide rapid polymerization kinetics and instant polymerase activation
- Reduced time to results without compromising qPCR data quality — the combination of Sso7d fusion polymerase and an optimized buffer significantly reduces the time required to obtain qPCR results without sacrificing data integrity
Applications and Uses of SsoAdvanced Universal Probes Supermix
- qPCR/real-time PCR
- Gene expression analysis
- Absolute quantification
- Genotyping (allelic discrimination)
- Mutation detection
- Pathogen detection
- Viral detection (load)
- Characterization of genetically modified organisms (GMO)
- Genetic profiling
Packaging Options
| Number of 20 μl Reactions | Total Volume | Number of Vials | Catalog # |
| 200 | 2 ml | 2 x 1 ml | 1725280EDU |
| 500 | 5 ml | 5 x 1 ml | 1725281EDU |
| 1000 | 10 ml | 10 x 1 ml | 1725282EDU |