iScript™ Select cDNA Synthesis Kit, 25 x 20 µl rxns #1708896EDU

iScript™ Select cDNA Synthesis Kit, 25 x 20 µl rxns

Overview

25 x 20 µl reactions, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water; for education use only (for research use, see 1708896)

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Description

Description

Use the iScript Select cDNA Synthesis Kit for high-fidelity, sensitive reverse transcription (RT-PCR) in cloning, RT-qPCR, next-generation sequencing, sequencing, and other applications in your classroom lab. This kit offers individually packaged reagents to give you flexible priming options and the ability to customize reaction setup for your specific application needs.

Key Features & Benefits

  • Minimizes introduction of base pair errors
  • Synthesizes long cDNAs greater than 8 kb
  • Save time and reduce errors during setup — less hands-on time and fewer pipetting steps compared to protocols for other high-fidelity reverse transcriptase kits
  • Detect low-level target genes and conserve RNA — broad linear dynamic range of total input RNA (1 µg–1 pg) with a highly efficient RNase H+ MMLV reverse transcriptase
  • Avoid skewed data from RNA degradation — potent blend of RNaseA inhibitor protects RNA during setup and reverse transcription

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