Description
Description
Use the iScript Reverse Transcription Supermix for RT-qPCR for fast synthesis of first-strand cDNA for direct use in real-time qPCR in your classroom lab. This single-tube supermix contains all the components for reverse transcription except RNA template.
Key Features & Benefits
- Combined oligo(dT) and random primers with RNase H+ MMLV reverse transcriptase for amplification of cDNA for genes with low expression levels
- Fast 26-minute 1-tube cDNA synthesis protocol
- 50 reactions of no-RT controls for optional detection of gDNA amplicon contamination
- Unbiased representation of 5' and 3' regions and a linear range of 1 µg–1 pg total input RNA
Related Products
- PCR, RT-PCR, and Real-Time PCR Reagents
- CFX Duet Real-Time PCR System for the Classroom
- CFX Opus Real-Time PCR Systems for the Classroom