iScript™ Reverse Transcription Supermix, 25 x 20 µl reactions #1708840EDU

iScript™ Reverse Transcription Supermix, 25 x 20 µl reactions

Overview

25 x 20 µl reactions, 100 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 50 reactions of no-RT control supermix; for education use only (for research use, see 1708840) 

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Description

Description

Use the iScript Reverse Transcription Supermix for RT-qPCR for fast synthesis of first-strand cDNA for direct use in real-time qPCR in your classroom lab. This single-tube supermix contains all the components for reverse transcription except RNA template.

Key Features & Benefits

  • Combined oligo(dT) and random primers with RNase H+ MMLV reverse transcriptase for amplification of cDNA for genes with low expression levels
  • Fast 26-minute 1-tube cDNA synthesis protocol
  • 50 reactions of no-RT controls for optional detection of gDNA amplicon contamination
  • Unbiased representation of 5' and 3' regions and a linear range of 1 µg–1 pg total input RNA

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