Description
Description
Use iScript RT-qPCR Sample Preparation Reagent to isolate total RNA from cultured cells to use for reverse-transcription quantitative PCR (RT-qPCR). This reagent delivers efficient cell lysis and RNA stabilization for sensitive qPCR without the complexities associated with traditional RNA sample preparation.
This catalog number is for purchases made for use in an educational setting. For use in research, please order 1708898.
Key Features & Benefits
- Simple and rapid procedure — one-buffer formulation contains all the necessary components to perform cell lysis and RNA stabilization in 5-10 minutes
- Excellent compatibility with qPCR directly from cells — sensitive detection of high-, medium-, and low-copy gene targets directly from cell lysates using real-time qPCR when combined with iScript reverse transcription and qPCR reagents
- Maximum data from very few cells — reagent enables multiplex real-time detection of up to 4 targets from as few as 10 cells
- Extended RNA shelf-life — isolated RNA is stable for up to 6 months when frozen
- Compatibility with different cell types — compatible with both suspension and adherent cell cultures