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iScript gDNA Clear cDNA Synthesis Kit, 25 x 20 µl reactions #1725034EDU

iScript gDNA Clear cDNA Synthesis Kit, 25 x 20 µl reactions

Overview

25 x 20 µl reactions, 100 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 200 µl no-RT control supermix; 12.5 µl DNase; 150 µl DNase buffer; for education use only (for research use, see 1725034)

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Description

Description

Use iScript gDNA Clear cDNA Synthesis Kit for easy and rapid generation of qPCR-ready cDNA that is free of genomic DNA (gDNA) for accurate gene expression analysis by real-time PCR.

This catalog number is for purchases made for use in an educational setting. For use in research, please order 1725034.

Features & Benefits

  • Effective gDNA removal in purified RNA samples
  • Proprietary DNase buffer maintains RNA integrity during gDNA removal
  • Convenient all-in-one, single-tube RT supermix
  • Ready-to-use no-RT control supermix for monitoring gDNA contamination
  • gDNA-free cDNA ready in within minutes

gDNA-Free cDNA Ready in 36 Minutes

This kit allows you to effectively remove contaminating gDNA and reliably synthesize cDNA with minimal hands-on time in an easy-to-follow protocol, resulting in cDNA free of genomic DNA in 36 minutes.

workflow for dna removal and cdna synthesis