Western Blot Kits
Bio-Rad offers a targeted selection of western blot kits, consumables and reagents designed for maximal sensitivity for protein quantitation. These products include binding proteins and conjugates, buffers, reagents, membranes, and other consumables.
Western Blot Detection Kits
Please be aware that there are other products in our protein quantification range, including consumables for gel electrophoresis and protein transfer, so if you cannot find exactly what you are looking for, do not hesitate to contact us, so we can help.
Selecting the elements that make up your perfect western blot kit has never been so easy. Below we have arranged the key elements of into three separate sections (kits, substrate & buffers, and secondary antibodies).
However, if you are unsure about what blotting membrane works best, don't know what gel type to use or how to visualize your results, then our Western Blotting Selection Tool is here to help.
Western Blot Kit Range
Immun-Star™ AP Chemiluminescence Kits
The highly sensitive Immun-Star AP Kit combines Bio-Rad's blotting reagents and expertise with CDP-Star chemiluminescence technology.
| Kit Options | Catalog |
|---|---|
| Immun-Star™ Goat Anti-Mouse (GAM)-AP Detection Kit | 1705010 |
| Immun-Star™ Goat Anti-Rabbit (GAR)-AP Detection Kit | 1705011 |
| Goat Anti-Rabbit (GAR)-AP Intro Kit | 1705014 |
Immun-Blot® AP Colorimetric Kits
AP-based colorimetric kits utilize 5-bromo-4-chloro-3-indolyl phosphate/Nitroblue Tetrazolium (BCIP/NBT), which reacts with AP to form a color precipitate. Can detect as little as 100 pg of protein.
| Kit Options | Catalog |
|---|---|
| (GAR) IgG (H+L)-AP Assay Kit | 1706460 |
| (GAM) IgG (H+L)-AP Assay Kit | 1706461 |
| AP Conjugate Substrate Kit | 1706432 |
Immun-Blot® Opti-4CN™ Colorimetric Kits
Bio-Rad's HRP-based colorimetric detection systems offer a choice of HRP-conjugated secondary antibodies and HRP-conjugated protein A or protein G using the detection reagent 4-chloro-1-naphthol II (4CN).
| Kit Options | Catalog |
|---|---|
| Opti-4CN Substrate Kit | 1708235 |
| Amplified Opti-4CN Substrate Kit | 1708238 |
| Amplified Opti-4CN (GAM) Detection Kit | 1708240 |
| Immun-Blot (GAR) IgG (H+L)-HRP Assay Kit | 1706463 |
| Immun-Blot (GAM) IgG (H+L)-HRP Assay Kit | 1706464 |
| HRP Conjugate Substrate Kit | 1706431 |
TeSeE Western Blot Kit
The TeSeE western blot kit is recognized worldwide by experts as an effective tool for confirmation of suspected TSE cases. Suitable for various animal species.
| Kit Options | Catalog |
|---|---|
| TeSeE Western Blot | 3551169 |
LYNX Rapid Plus Rhodamine Antibody Conjugation Kit
This antibody conjugation kit enables the ultra rapid conjugation of a pre-prepared lyophilized mixture containing Rhodamine label to an antibody or protein. Activation of proprietary reagents within the antibody-label solution results in the coupling of Rhodamine to the antibody.
| Kit Options | Catalog |
|---|---|
| LYNX Rapid Plus Rhodamine Antibody Conjugation Kit | LNK253RHO |
Buffers & Substrates
EveryBlot Blocking Buffer
Rapid blocking buffer that blocks western blots in 5 minutes and enhances sensitivity, regardless of the detection method. Also ELISA-compatible, reducing blocking time from 1 hour to seconds.
| Kit Options | Catalog |
|---|---|
| EveryBlot Blocking Buffer | 12010020 |
Clarity Western ECL Substrate
Use Clarity Western ECL Substrate with any HRP-conjugated secondary antibody for digital or film-based chemiluminescent western blot imaging. Mid-femtogram-level sensitivity with 24-hour signal duration.
| Kit Options | Catalog |
|---|---|
| Clarity Western ECL Substrate (20 ml) | 1705060S |
| Clarity Western ECL Substrate (100 ml) | 1705060 |
| Clarity Western ECL Substrate (500 ml) | 1705061 |
Clarity Max ECL Substrate
For detection of low abundant proteins, use Clarity Max Western ECL Substrate with any HRP-conjugated secondary antibody for digital or film-based chemiluminescent western blot imaging. Low-femtogram-level sensitivity.
| Kit Options | Catalog |
|---|---|
| Clarity Max ECL Substrate (20 ml) | 1705062S |
| Clarity Max ECL Substrate (100 ml) | 1705062 |
StarBright Blue Fluorescent Secondary Antibodies
StarBright Blue 700 Fluorescent Secondary Antibodies
Conjugated to a high-yield fluorophore, for ultra-sensitive fluorescence detection with very low background of single or multiple proteins in one blot without stripping or reprobing.
| Kit Options | Catalog |
|---|---|
| Goat anti-mouse — 80 µl | 12004159 |
| Goat anti-mouse — 400 µl | 12004158 |
| Goat anti-rabbit — 80 µl | 12004162 |
| Goat anti-rabbit — 400 µl | 12004161 |
StarBright Blue 520 Fluorescent Secondary Antibodies
Conjugated to a high-yield fluorophore, for ultra-sensitive fluorescence detection with very low background of single or multiple proteins in one blot without stripping or reprobing.
| Kit Options | Catalog |
|---|---|
| Goat anti-mouse — 80 µl | 12004159 |
| Goat anti-mouse — 400 µl | 12004158 |
| Goat anti-rabbit — 80 µl | 12004162 |
| Goat anti-rabbit — 400 µl | 12004161 |
Looking for SDS-PAGE gels, tanks, power supplies or premade running buffers?
Facilitating Better Transfer
Trans-Blot Turbo Transfer System
The Trans-Blot Turbo Transfer system is a rapid protein transfer apparatus that can transfer proteins to membranes in as little as 3 minutes. Trans-Blot Turbo transfer packs and RTA kits provide greater transfer efficiency in less time.
Western Blotting Membranes & Filter Paper
Our Nitrocellulose membranes, PVDF membranes, low-fluorescence membranes, and filter paper for western blotting applications are available in various formats including precut sizes that match standard gel sizes.
Western Blot Resources
All of the above products and western blot kits should fulfill the requirements of your western blot workflow. Below we have provided some handy resources to assist further with your experiments and research.
Western Blot University
Courses designed to make you a western blotting expert.
Western Blot Learning Center
The Western Blot Learning Center is a complete reference on all of the steps of western blotting, includes practical theory, protocols, and recommendations on how to make your blots better from experts.
Western Blot Selector Tool
Use the western blot selector tool to find the best reagents and consumables for your next experiment.
Western Blot Protocol Library
Find various protocols that fit your lab's unique needs from reagents, detection modes, lab equipment, and more. Our protocols provide step-by-step details.
Western Blot Doctor
Get self-guided instructions on how to troubleshoot common western blotting problems and challenges.
Stain-Free Western Blotting Workflow
Check out our Stain-Free Western Blotting Workflow, a streamlined approached to producing better western blotting results.
Free 26-Lane Electrophoresis & Western Blotting Layout Post-It
Request a complete guide to Bio-Rad Stain-Free western blotting ranging from tips, techniques, and publications.
Related Products
Validated Western Blot Antibodies
The PrecisionAb Antibody range are rigorously tested western blotting antibodies to ensure specificity and reproducibility.
Multiplex Western Blot Reagents
Multiplex fluorescent western blotting enables the simultaneous analysis of multiple proteins on the same blot without stripping and reprobing.
ChemiDoc Imaging Systems Family
One of the leading western blot imaging systems
Protein Gels
Choose SDS-PAGE and native-PAGE gels, convert to TGX Precast Gels, or choose specialized gel chemistries including stain-free.