
Overview
The Trans-Blot Turbo Transfer System is a high-performance western blotting transfer system designed to provide rapid transfers with high efficiency. The system enables blot transfer of protein in as little as 3 minutes without sacrificing performance when compared with traditional tank protein blotting. The Trans-Blot Turbo Transfer Packs include an optimized buffer, membrane, and filter paper combination that provides superior blot transfers when paired with the Trans-Blot Turbo blotting apparatus. Protein blotting consumables are available in a ready-to-use, pre-packaged stack or in bulk quantities that require assembly.
Note: The Trans-Blot Turbo Transfer System is for research use only, not intended for diagnostic purposes
Features and Benefits of the Trans-Blot Turbo Transfer System
- Rapid blot transfer — 3 min transfers of mini or midi gels
- High throughput — can transfer 1–4 mini or 1–2 midi gels in a single run
- Greater transfer efficiency — higher transfer efficiency than other protein blotting methods
- Flexible design — allows the user to customize transfer conditions and is compatible with traditional semi-dry consumables
- Environmentally friendly — environmentally safe consumables eliminate disposal cost
- Recommended for Stain-Free Western Blotting — permits a streamlined workflow
Applications and Uses of the Trans-Blot Turbo Transfer System
This protein blotting system is a powerful tool in the western blotting workflow, enabling the visualization and identification of proteins for downstream analysis. The Trans-Blot Turbo transfer system can be used in the following applications:

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- Separation, transfer and analysis (Stain-Free Western Blotting Workflow)
- Protein blot transfers
- Evaluation of protein expression levels
- Immunological or biochemical analyses
- Protein-protein or protein-ligand interactions
Trans-Blot Turbo Consumables
Trans-Blot Turbo Transfer membranes and filter paper come in:
- Preassembled, ready-to-use packs - time-saving, efficient, and eliminates the need to assemble and wet the transfer stack
- Ready-To-Assemble (RTA) Transfer Kits - economical option for high-throughput labs that includes optimized transfer buffer, membrane and filter paper combination
- Individual Trans-Blot Turbo Components - versatile, tailored solution that allows you to purchase only what you need, cutting down on excess
Preassembled Transfer Packs (10 Transfers) | RTA Transfer Kits (40 Transfers, contains membranes, filter paper & transfer buffer) | Individual RTA Transfer Components | ||||
---|---|---|---|---|---|---|
Mini | Midi | Mini | Midi | Mini | Midi | |
0.2 µm PVDF | 1704156 | 1704157 | 1704272 | 1704273 | 12023954 | 12023927 |
0.45 µm LF (low fluorescence) PVDF | – | – | 1704274 | 1704275 | 1620174 | 1620175 |
0.2 µm Nitrocellulose | 1704158 | 1704159 | 1704270 | 1704271 | 12023955 | 12023945 |
Filter Paper | – | – | – | – | 12023835 | 12023956 |
Trans-Blot Turbo 5x Transfer Buffer, 1L | – | – | – | – | 10026938 | 10026938 |
Note: For optimal fluorescent western blotting detection, select the low-fluorescence PVDF membrane option Trans-Blot Turbo RTA Transfer Kit or individual RTA transfer component (Immun-Blot LF-PVDF: 1620261 Mini, 1620263 Midi).
Use Bio-Rad's Precision Plus Protein WesternC Standards and Clarity Western ECL Substrate for chemiluminescence detection on both film and digital imagers. See how the Trans-Blot Turbo transfer system fits into the Stain-Free Western Blotting to streamline your western blotting process.
See the ordering information for Mini-PROTEAN TGX Precast Gels and Criterion TGX Precast Gels to select the best gel for your application.
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Blotting instrument, includes base, 2 cassettes to hold 1–2 midi or up to 4 mini blotting sandwiches, blot roller





Accessories


Kits & Reagents

1 Liter 5x Trans-Blot Turbo Transfer Buffer compatible with the TBT Ready-To-Assemble Transfer Kits





Ready-to-assemble transfer kit includes 40 mini-sized 0.2 µm nitrocellulose membranes (7 x 8.5 cm), 80 transfer stacks, 1 L 5x transfer buffer, and 2 gel trays for wetting and equilibrating membranes and transfer stacks

Ready-to-assemble transfer kit includes 40 midi-sized 0.2 µm nitrocellulose membranes (8.5 x 13.5 cm), 80 transfer stacks, 2 L 5x transfer buffer, and 2 gel trays for wetting and equilibrating membranes and transfer stacks




Ready-to-assemble transfer kit includes 40 midi-sized 0.45 µm LF PVDF membranes (8.5 x 13.5 cm), 80 transfer stacks, 2 L 5x transfer buffer, and 2 gel trays for wetting and equilibrating membranes and transfer stacks

80 mini-sized Filter Paper (7 x 8.5 cm) stacks, compatible with the Ready-To-Assemble TBT Transfer Kits, requires assembly

40 midi-sized 0.2 µm PVDF membranes (8.5 x 13.5 cm), compatible with the Ready-To-Assemble TBT Transfer Kits, dry membranes require rehydration

40 mini-sized 0.2 µm PVDF membranes (7 x 8.5 cm), compatible with the Ready-To-Assemble TBT Transfer Kits, dry membranes require rehydration

80 midi-sized Filter Paper (8.5 x 13.5 cm), compatible with the Ready-To-Assemble TBT Transfer Kits, requires assembly

40 mini-sized 0.2 µm Nitrocellulose membranes (7 x 8.5 cm), compatible with the Ready-To-Assemble TBT Transfer Kits, dry membranes require rehydration

40 midi-sized 0.2 µm nitrocellulose membranes (8.5 x 13.5 cm), compatible with the Ready-To-Assemble TBT Transfer Kits, dry membranes require rehydration