
Description
Description
SureBeads™ Protein A Magnetic Beads and Magnetic Racks are designed for quick and easy immunoprecipitation (IP) and protein complex pull-down. SureBeads Magnetic Beads contain a superparamagnetic core, which enables rapid and highly efficient immunoprecipitation without the need for centrifugation.
Immunoprecipitation in five easy steps:
Download the SureBeads protocol for more information on immunoprecipitation with the SureBeads System.
Trial Size Available |
Try a SureBeads Trial Pack. Includes 1 ml conjugated magnetic beads and a mini magnetic rack for 4 microcentrifuge tubes.
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Features and Benefits of SureBeads Protein A Magnetic Beads
- Faster IP — magnetization is faster than centrifugation
- Easier IP — beads cling magnetically to the side wall of the tube, not the bottom, for easier pipetting
- Reliable IP — consistent IgG binding capacity ensures accurate, reproducible results. Less nonspecific binding than agarose or Sepharose Beads
- Save on antibody costs — unique surface chemistry enables you to use less antibody
- Easy to use — ergonomically designed SureBeads Rack with separable magnets works in seconds to magnetize the beads
Applications and Uses of SureBeads Protein A Magnetic Beads
- Immunoprecipitation for protein isolation
- Co-immunoprecipitation (Co-IP) or protein complex pull-down
- High-affinity binding of human, rat, and mouse IgGs
Packaging Options
Conjugated Protein | Bead Suspension Volume | Rack Size (Number of Tubes) |
Catalog # |
G | 1 ml | — | 161-4021 |
G | 3 ml | — | 161-4023 |
G | 1 ml | 4* | 161-4821 |
A | 1 ml | — | 161-4011 |
A | 3 ml | — | 161-4013 |
A | 1 ml | 4* | 161-4811 |
A/G | 2 x 3 ml | 16 | 161-4833 |
G/G | 2 x 3 ml | 16 | 161-4823 |
A/A | 2 x 3 ml | 16 | 161-4813 |
16-tube magnetic rack | — | 16 | 161-4916 |
*Trial kit
More Information
Protein A/G Immunoprecipitation Guide
Protein A and protein G have different binding affinities for various species and subclasses of monoclonal and polyclonal antibodies. Use the table below to select the best SureBeads products for your needs.
Monoclonal | Protein A | Protein G |
Human IgG1 | +++ | +++ |
Human IgG2 | +++ | +++ |
Human IgG3 | – | +++ |
Human IgG4 | +++ | +++ |
Mouse IgG1 | + | +++ |
Mouse IgG2a | +++ | +++ |
Mouse IgG2b | ++ | ++ |
Mouse IgG3 | ++ | ++ |
Rat IgG1 | – | + |
Rat IgG2aa | – | +++ |
Rat IgG2b | – | ++ |
Rat IgG2c | + | ++ |
Polyclonal | Protein A | Protein G |
Rabbit | +++ | ++ |
Cow | ++ | +++ |
Horse | ++ | +++ |
Goat | – | ++ |
Guinea pig | +++ | ++ |
Sheep | +/- | ++ |
Pig | ++ | ++ |
Rat | +/- | ++ |
Mouse | ++ | ++ |
Chicken | – | + |
Human IgG | +++ | +++ |
Human IgM | – | + |
Human IgD | – | + |
Human IgA | – | + |
– No binding
+ Weak binding
++ Moderate binding
+++ Strong binding
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