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Description
Description
SureBeads™ Protein A Magnetic Beads and Magnetic Racks are designed for quick and easy immunoprecipitation (IP) and protein complex pull-down. SureBeads Magnetic Beads contain a superparamagnetic core, which enables rapid and highly efficient immunoprecipitation without the need for centrifugation.
Immunoprecipitation in five easy steps:
Download the SureBeads protocol for more information on immunoprecipitation with the SureBeads System.
| Trial Size Available |
| Try a SureBeads Trial Pack. Includes 1 ml conjugated magnetic beads and a mini magnetic rack for 4 microcentrifuge tubes.
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Features and Benefits of SureBeads Protein A Magnetic Beads
- Faster IP — magnetization is faster than centrifugation
- Easier IP — beads cling magnetically to the side wall of the tube, not the bottom, for easier pipetting
- Reliable IP — consistent IgG binding capacity ensures accurate, reproducible results. Less nonspecific binding than agarose or Sepharose Beads
- Save on antibody costs — unique surface chemistry enables you to use less antibody
- Easy to use — ergonomically designed SureBeads Rack with separable magnets works in seconds to magnetize the beads
Applications and Uses of SureBeads Protein A Magnetic Beads
- Immunoprecipitation for protein isolation
- Co-immunoprecipitation (Co-IP) or protein complex pull-down
- High-affinity binding of human, rat, and mouse IgGs
Packaging Options
| Conjugated Protein | Bead Suspension Volume | Rack Size (Number of Tubes) |
Catalog # |
| G | 1 ml | — | 161-4021 |
| G | 3 ml | — | 161-4023 |
| G | 1 ml | 4* | 161-4821 |
| A | 1 ml | — | 161-4011 |
| A | 3 ml | — | 161-4013 |
| A | 1 ml | 4* | 161-4811 |
| A/G | 2 x 3 ml | 16 | 161-4833 |
| G/G | 2 x 3 ml | 16 | 161-4823 |
| A/A | 2 x 3 ml | 16 | 161-4813 |
| 16-tube magnetic rack | — | 16 | 161-4916 |
*Trial kit
More Information
Protein A/G Immunoprecipitation Guide
Protein A and protein G have different binding affinities for various species and subclasses of monoclonal and polyclonal antibodies. Use the table below to select the best SureBeads products for your needs.
| Monoclonal | Protein A | Protein G |
| Human IgG1 | +++ | +++ |
| Human IgG2 | +++ | +++ |
| Human IgG3 | – | +++ |
| Human IgG4 | +++ | +++ |
| Mouse IgG1 | + | +++ |
| Mouse IgG2a | +++ | +++ |
| Mouse IgG2b | ++ | ++ |
| Mouse IgG3 | ++ | ++ |
| Rat IgG1 | – | + |
| Rat IgG2aa | – | +++ |
| Rat IgG2b | – | ++ |
| Rat IgG2c | + | ++ |
| Polyclonal | Protein A | Protein G |
| Rabbit | +++ | ++ |
| Cow | ++ | +++ |
| Horse | ++ | +++ |
| Goat | – | ++ |
| Guinea pig | +++ | ++ |
| Sheep | +/- | ++ |
| Pig | ++ | ++ |
| Rat | +/- | ++ |
| Mouse | ++ | ++ |
| Chicken | – | + |
| Human IgG | +++ | +++ |
| Human IgM | – | + |
| Human IgD | – | + |
| Human IgA | – | + |
– No binding
+ Weak binding
++ Moderate binding
+++ Strong binding
Related Products
Specifications
Specifications
Bead size
2.4–3.4 µm
Bead magnetic content
14–28%
Typical IgG binding capacity
≥6 µg/mg
Solid content in slurry
1.0–1.2%
Protein A/G content
9–12 µg/mg
Dispersion medium
TBS + 0.05% Tween 20 + 0.09% sodium azide
Ordering
Ordering
items
Use the filters below to refine results!
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(Discontinued) SureBeads™ Trial Kit Protein A
1614811
Trial kit for protein A conjugated magnetic beads for immunoprecipitation, contains 1 ml bead suspension (10 mg beads/ml suspension) and a 4-tube magnetic rack
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(Discontinued) SureBeads™ Protein A Magnetic Beads, 3 ml
1614013
3 ml, suspension of protein A conjugated magnetic immunoprecipitation beads (10 mg beads/ml)
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(Discontinued) SureBeads™ Starter Kit Protein A and G
1614833
Starter kit for immunoprecipitation with protein A and G conjugated magnetic beads; 3 ml protein G bead suspension, 3 ml protein A bead suspension (10 mg beads/ml suspension), and 16-tube magnetic rack
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(Discontinued) SureBeads™ Starter Kit Protein A
1614813
Starter kit for immunoprecipitation with protein A conjugated magnetic beads, contains 2 x 3 ml protein A beads suspension (10 mg beads/ml suspension) and a 16-tube magnetic rack
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(Discontinued) SureBeads™ Protein A Magnetic Beads, 1 ml
1614011
1 ml, suspension of protein A conjugated magnetic immunoprecipitation beads (10 mg beads/ml)
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(Discontinued) 16-Tube SureBeads™ Magnetic Rack
1614916
Rack with high-strength magnets for rapid magnetic bead separation. Supports 16 x 1.5 ml microcentrifuge tubes or 2 x 15 ml conical tubes. Use with SureBeads Magnetic Beads.
Documents
Documents
Login Required Number Description Options
7110
Chromatin Immunoprecipitation (ChIP) with SureBeads Protein A and G Magnetic Beads
6594
Immunoprecipitation Performance of SureBeads™ Protein A Magnetic Beads vs. Protein A Agarose Beads, Rev A
10041689
SureBeads™ Magnetic Beads for Immunoprecipitation Product Insert, Rev A
10043651
SureBeads™ Magnetic Beads Standard Immunoprecipitation Protocol, Rev A
7087
Protein Sample Preparation Publications List
