SureBeads™ Protein A Magnetic Beads

Overview

SureBeads Protocol

SureBeads™ Immunoprecipitation Protocol

Standard immunoprecipitation protocol using the SureBeads System.

SureBeads System Video Demo

SureBeads™ Magnetic Bead System for Immunoprecipitation

SureBeads Magnetic Beads vs. Agarose Beads

Performance of SureBeads Magnetic Beads vs. Agarose Beads

SureBeads Protein A Magnetic Beads pull down approximately four times more NAD(P)H dehydrogenase [quinone] 1 (NQO1) protein from HeLa cell lysates than do equal amounts of protein A agarose beads, improving yields and reducing antibody consumption.

Comparison of immunoprecipitation (IP) performance of SureBeads magnetic beads and agarose beads

Comparison of the immunoprecipitation (IP) performance of SureBeads Magnetic Beads and agarose beads.

Read the complete testing protocol in Immunoprecipitation Performance of SureBeads™ Protein A Magnetic Beads vs. Protein A Agarose Beads (Bulletin 6594).

Download PDF: Immunoprecipitation Performance of SureBeads™ Protein A Magnetic Beads vs. Protein A Agarose Beads, Rev A (Bulletin 6594)

Protein A/G Binding Affinities

Antibody Binding Affinities of Protein A and Protein G

Monoclonal	Protein A	Protein G
Human IgG₁	+++	+++
Human IgG₂	+++	+++
Human IgG₃	–	+++
Human IgG₄	+++	+++
Mouse IgG₁	+	+++
Mouse IgG_2a	+++	+++
Mouse IgG_2b	++	++
Mouse IgG₃	++	++
Rat IgG₁	–	+
Rat IgG_2aa	–	+++
Rat IgG_2b	–	++
Rat IgG_2c	+	++
Polyclonal	Protein A	Protein G
Rabbit	+++	++
Cow	++	+++
Horse	++	+++
Goat	–	++
Guinea pig	+++	++
Sheep	+/-	++
Pig	++	++
Rat	+/-	++
Mouse	++	++
Chicken	–	+
Human IgG	+++	+++
Human IgM	–	+
Human IgD	–	+
Human IgA	–	+

– No binding
+ Weak binding
++ Moderate binding
+++ Strong binding

Binding affinities of protein A and G to various species and subclasses of monoclonal and polyclonal antibodies.

SureBeads™ Protein A Magnetic Beads enable fast, easy, consistent immunoprecipitation without centrifugation.
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Description
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Description

SureBeads™ Protein A Magnetic Beads and Magnetic Racks are designed for quick and easy immunoprecipitation (IP) and protein complex pull-down. SureBeads Magnetic Beads contain a superparamagnetic core, which enables rapid and highly efficient immunoprecipitation without the need for centrifugation.

Immunoprecipitation in five easy steps:

Immunoprecipitation in five easy steps

Download the SureBeads protocol for more information on immunoprecipitation with the SureBeads System.

Trial Size Available

Try a SureBeads Trial Pack.
Includes 1 ml conjugated magnetic beads and a mini magnetic rack for 4 microcentrifuge tubes.

Features and Benefits of SureBeads Protein A Magnetic Beads

Faster IP — magnetization is faster than centrifugation
Easier IP — beads cling magnetically to the side wall of the tube, not the bottom, for easier pipetting
Reliable IP — consistent IgG binding capacity ensures accurate, reproducible results. Less nonspecific binding than agarose or Sepharose Beads
Save on antibody costs — unique surface chemistry enables you to use less antibody
Easy to use — ergonomically designed SureBeads Rack with separable magnets works in seconds to magnetize the beads

Applications and Uses of SureBeads Protein A Magnetic Beads

Immunoprecipitation for protein isolation
Co-immunoprecipitation (Co-IP) or protein complex pull-down
High-affinity binding of human, rat, and mouse IgGs

Packaging Options

Conjugated Protein	Bead Suspension Volume	Rack Size (Number of Tubes)	Catalog #
G	1 ml	—	161-4021
G	3 ml	—	161-4023
G	1 ml	4*	161-4821
A	1 ml	—	161-4011
A	3 ml	—	161-4013
A	1 ml	4*	161-4811
A/G	2 x 3 ml	16	161-4833
G/G	2 x 3 ml	16	161-4823
A/A	2 x 3 ml	16	161-4813
16-tube magnetic rack	—	16	161-4916

*Trial kit

More Information

Protein A/G Immunoprecipitation Guide

Protein A and protein G have different binding affinities for various species and subclasses of monoclonal and polyclonal antibodies. Use the table below to select the best SureBeads products for your needs.

Monoclonal	Protein A	Protein G
Human IgG₁	+++	+++
Human IgG₂	+++	+++
Human IgG₃	–	+++
Human IgG₄	+++	+++
Mouse IgG₁	+	+++
Mouse IgG_2a	+++	+++
Mouse IgG_2b	++	++
Mouse IgG₃	++	++
Rat IgG₁	–	+
Rat IgG_2aa	–	+++
Rat IgG_2b	–	++
Rat IgG_2c	+	++
Polyclonal	Protein A	Protein G
Rabbit	+++	++
Cow	++	+++
Horse	++	+++
Goat	–	++
Guinea pig	+++	++
Sheep	+/-	++
Pig	++	++
Rat	+/-	++
Mouse	++	++
Chicken	–	+
Human IgG	+++	+++
Human IgM	–	+
Human IgD	–	+
Human IgA	–	+

– No binding
+ Weak binding
++ Moderate binding
+++ Strong binding

Specifications

Bead size	2.4–3.4 µm
Bead magnetic content	14–28%
Typical IgG binding capacity	≥6 µg/mg
Solid content in slurry	1.0–1.2%
Protein A/G content	9–12 µg/mg
Dispersion medium	TBS + 0.05% Tween 20 + 0.09% sodium azide

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SureBeads™ Trial Kit Protein A

1614811
Trial kit for protein A conjugated magnetic beads for immunoprecipitation, contains 1 ml bead suspension (10 mg beads/ml suspension) and a 4-tube magnetic rack

This product is being discontinued soon. Please contact customer service for more information.

SureBeads™ Protein A Magnetic Beads, 3 ml

1614013
3 ml, suspension of protein A conjugated magnetic immunoprecipitation beads (10 mg beads/ml)

1614013 /templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Magnetic-Beads-Racks-Immunoprecipitation/161-4013_1401321571.xml 1614013 /default/main/bio-rad/WORKAREA/default 05/28/14 04:54 PM en_US jcjsxz4a 2017-11-14 07:08:58 true true internet/product_SKU jcjsxz4a templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Magnetic-Beads-Racks-Immunoprecipitation/161-4013_1401321571.xml LSR 2019-05-04 16:23:47 /default/main/bio-rad/STAGING N /default/main/bio-rad en 05/28/24 04:58 PM 1556043807434 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 16:23:47 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Magnetic-Beads-Racks-Immunoprecipitation/161-4013_1401321571.xml templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Magnetic-Beads-Racks-Immunoprecipitation/161-4013_1401321571.xml 1614013-surebeads-protein-magnetic-beads-3-ml 1614013 SureBeadsProteinAMagneticBeads3ml SureBeads™ Protein A Magnetic Beads, 3 ml 3 ml, suspension of protein A conjugated magnetic immunoprecipitation beads (10 mg beads/ml) /webroot/web/images/lsr/products/protein_sample_preparation/sku_thumb/global/surebeads-protein-a-magnetic-beads-3ml-sku-161-4013-thumb.jpg /webroot/web/images/lsr/products/protein_sample_preparation/sku_view/global/surebeads-protein-a-magnetic-beads-3ml-sku-161-4013-detail.jpg preview ,VN tommy a SureBeads Protein A Magnetic Beads 1 ml #161-4011 | Life Science Research | Bio-Rad surebeads, magnetic beads, immunoprecipitation, protein isolation, protein A 05/28/14 04:54 PM 05/28/24 04:58 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Protein-Sample-Preparation/Magnetic-Beads-Racks-Immunoprecipitation N 0 <p>Use SureBeads Protein A Magnetic Beads for fast, easy, and reliable bioseparations: immunoprecipitation (IP), co-immunoprecipitation (co-IP), and protein pull-down assays.</p> <p>Superparamagnetic beads with a surface-activated hydrophilic polymer and chemically conjugated protein A bind the Fc regions of immunoglobulins with high specificity.</p> <ul> <li>Faster IP using magnetization instead of centrifugation</li> <li>Unique bead surface chemistry ensures optimal antibody orientation for increased antigen binding efficiency</li> <li>High IgG binding affinity with low nonspecific binding and reduced antibody consumption</li> <li>Beads cling magnetically to the side wall of the tube, not the bottom, for easier wash pipetting</li> </ul> <h2 class="descHeader">Packaging Options</h2> <h2> <table class="pd_table" style="margin: 0 0 5px 10px; border-bottom: 1px solid #000000;" border="0"> <tbody> <tr> <td style="border-top: 1px solid #000000;"><strong>Conjugated Protein</strong></td> <td style="border-top: 1px solid #000000;" align="center"><strong>Bead Suspension Volume</strong></td> <td style="border-top: 1px solid #000000;" align="center"><strong>Rack Size</strong><br /> (Number of Tubes)</td> <td style="border-top: 1px solid #000000;" align="center"><strong>Catalog #</strong></td> </tr> <tr class="pd_colorbackground"> <td style="border-top: 1px solid #000000;" valign="top">G</td> <td style="border-top: 1px solid #000000;" align="center" valign="top">1 ml</td> <td style="border-top: 1px solid #000000;" align="center" valign="top">—</td> <td style="border-top: 1px solid #000000;" align="center" valign="top"><a style="font-size: 11px" href="/en-us/sku/1614021-surebeads-protein-g-magnetic-beads-1-ml?ID=1614021">161-4021</a></td> </tr> <tr> <td valign="top">G</td> <td align="center" valign="top">3 ml</td> <td align="center" valign="top">—</td> <td align="center" valign="top"><a style="font-size: 11px" href="/en-us/sku/1614023-surebeads-protein-g-magnetic-beads-3-ml?ID=1614023">161-4023</a></td> </tr> <tr class="pd_colorbackground"> <td valign="top">G</td> <td align="center" valign="top">1 ml</td> <td align="center" valign="top">4*</td> <td align="center" valign="top"><a style="font-size: 11px" href="/en-us/sku/1614821-surebeads-protein-g-magnetic-beads-trial-kit?ID=1614821">161-4821</a></td> </tr> <tr> <td valign="top">A</td> <td align="center" valign="top">1 ml</td> <td align="center" valign="top">—</td> <td align="center" valign="top"><a style="font-size: 11px" href="/en-us/sku/1614011-surebeads-protein-magnetic-beads-1-ml?ID=1614011">161-4011</a></td> </tr> <tr class="pd_colorbackground"> <td valign="top">A</td> <td align="center" valign="top">3 ml</td> <td align="center" valign="top">—</td> <td align="center" valign="top">161-4013</td> </tr> <tr> <td valign="top">A</td> <td align="center" valign="top">1 ml</td> <td align="center" valign="top">4*</td> <td align="center" valign="top"><a style="font-size: 11px" href="/en-us/sku/1614811-surebeads-protein-magnetic-beads-trial-kit?ID=1614811">161-4811</a></td> </tr> <tr class="pd_colorbackground"> <td valign="top">A/G</td> <td align="center" valign="top">2 x 3 ml</td> <td align="center" valign="top">16</td> <td align="center" valign="top"><a style="font-size: 11px" href="/en-us/sku/1614833-surebeads-immunoprecipitation-starter-kit-g?ID=1614833">161-4833</a></td> </tr> <tr> <td valign="top">G/G</td> <td align="center" valign="top">2 x 3 ml</td> <td align="center" valign="top">16</td> <td align="center" valign="top"><a style="font-size: 11px" href="/en-us/sku/1614823-surebeads-immunoprecipitation-starter-kit-g-g?ID=1614823">161-4823</a></td> </tr> <tr class="pd_colorbackground"> <td valign="top">A/A</td> <td align="center" valign="top">2 x 3 ml</td> <td align="center" valign="top">16</td> <td align="center" valign="top"><a style="font-size: 11px" href="/en-us/sku/1614813-surebeads-immunoprecipitation-starter-kit?ID=1614813">161-4813</a></td> </tr> <tr> <td colspan="2" valign="top">16-tube magnetic rack</td> <td align="center" valign="top">16</td> <td align="center" valign="top"><a style="font-size: 11px" href="/en-us/sku/1614916-16-tube-surebeads-magnetic-rack?ID=1614916">161-4916</a></td> </tr> </tbody> </table> <p class="caption">*Trial kit</p> </h2> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/category/protein-electrophoresis-blotting">Protein Electrophoresis and Western Blotting</a></li> <li><a href="/en-us/category/protein-sample-preparation">Protein Sample Preparation</a></li> </ul> <h2 class="descHeader">More Information</h2> <p>Protein A and protein G have different binding affinities for various species and subclasses of monoclonal and polyclonal antibodies. Use the table below to select the best SureBeads products for your needs.</p> <h2 class="descHeader" style="clear: both">Protein A/G Immunoprecipitation Guide</h2> <table class="pd_table" style="margin: 0 0 5px 10px; border-bottom: 1px solid #000000;" border="0"> <tbody> <tr> <td style="border-top: 1px solid #000000;" valign="top"><strong>Monoclonal</strong></td> <td style="border-top: 1px solid #000000;" align="center" valign="top"><strong>Protein A</strong></td> <td style="border-top: 1px solid #000000;" align="center" valign="top"><strong>Protein G</strong></td> </tr> <tr class="pd_colorbackground"> <td style="border-top: 1px solid #000000;" valign="top">Human IgG<sub>1</sub></td> <td style="border-top: 1px solid #000000;" align="center" valign="top">+++</td> <td style="border-top: 1px solid #000000;" align="center" valign="top">+++</td> </tr> <tr> <td valign="top">Human IgG<sub>2</sub></td> <td align="center" valign="top">+++</td> <td align="center" valign="top">+++</td> </tr> <tr class="pd_colorbackground"> <td valign="top">Human IgG<sub>3</sub></td> <td align="center" valign="top">–</td> <td align="center" valign="top">+++</td> </tr> <tr> <td valign="top">Human IgG<sub>4</sub></td> <td align="center" valign="top">+++</td> <td align="center" valign="top">+++</td> </tr> <tr class="pd_colorbackground"> <td valign="top">Mouse IgG<sub>1</sub></td> <td align="center" valign="top">+</td> <td align="center" valign="top">+++</td> </tr> <tr> <td valign="top">Mouse IgG<sub>2a</sub></td> <td align="center" valign="top">+++</td> <td align="center" valign="top">+++</td> </tr> <tr class="pd_colorbackground"> <td valign="top">Mouse IgG<sub>2b</sub></td> <td align="center" valign="top">++</td> <td align="center" valign="top">++</td> </tr> <tr> <td valign="top">Mouse IgG<sub>3</sub></td> <td align="center" valign="top">++</td> <td align="center" valign="top">++</td> </tr> <tr class="pd_colorbackground"> <td valign="top">Rat IgG<sub>1</sub></td> <td align="center" valign="top">–</td> <td align="center" valign="top">+</td> </tr> <tr> <td valign="top">Rat IgG<sub>2a</sub>a</td> <td align="center" valign="top">–</td> <td align="center" valign="top">+++</td> </tr> <tr class="pd_colorbackground"> <td valign="top">Rat IgG<sub>2b</sub></td> <td align="center" valign="top">–</td> <td align="center" valign="top">++</td> </tr> <tr> <td valign="top">Rat IgG<sub>2c</sub></td> <td align="center" valign="top">+</td> <td align="center" valign="top">++</td> </tr> <tr> <td style="border-top: 1px solid #000000; border-bottom: 1px solid #000000;" valign="top"><strong>Polyclonal</strong></td> <td style="border-top: 1px solid #000000; border-bottom: 1px solid #000000;" align="center" valign="top"><strong>Protein A</strong></td> <td style="border-top: 1px solid #000000; border-bottom: 1px solid #000000;" align="center" valign="top"><strong>Protein G</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">Rabbit</td> <td align="center" valign="top">+++</td> <td align="center" valign="top">++</td> </tr> <tr> <td valign="top">Cow</td> <td align="center" valign="top">++</td> <td align="center" valign="top">+++</td> </tr> <tr class="pd_colorbackground"> <td valign="top">Horse</td> <td align="center" valign="top">++</td> <td align="center" valign="top">+++</td> </tr> <tr> <td valign="top">Goat</td> <td align="center" valign="top">–</td> <td align="center" valign="top">++</td> </tr> <tr class="pd_colorbackground"> <td valign="top">Guinea pig</td> <td align="center" valign="top">+++</td> <td align="center" valign="top">++</td> </tr> <tr> <td valign="top">Sheep</td> <td align="center" valign="top">+/-</td> <td align="center" valign="top">++</td> </tr> <tr class="pd_colorbackground"> <td valign="top">Pig</td> <td align="center" valign="top">++</td> <td align="center" valign="top">++</td> </tr> <tr> <td valign="top">Rat</td> <td align="center" valign="top">+/-</td> <td align="center" valign="top">++</td> </tr> <tr class="pd_colorbackground"> <td valign="top">Mouse</td> <td align="center" valign="top">++</td> <td align="center" valign="top">++</td> </tr> <tr> <td valign="top">Chicken</td> <td align="center" valign="top">–</td> <td align="center" valign="top">+</td> </tr> <tr class="pd_colorbackground"> <td valign="top">Human IgG</td> <td align="center" valign="top">+++</td> <td align="center" valign="top">+++</td> </tr> <tr> <td valign="top">Human IgM</td> <td align="center" valign="top">–</td> <td align="center" valign="top">+</td> </tr> <tr class="pd_colorbackground"> <td valign="top">Human IgD</td> <td align="center" valign="top">–</td> <td align="center" valign="top">+</td> </tr> <tr> <td valign="top">Human IgA</td> <td align="center" valign="top">–</td> <td align="center" valign="top">+</td> </tr> </tbody> </table> <p class="caption">– No binding<br />+ Weak binding<br />++ Moderate binding<br />+++ Strong binding</p>  /webroot/web/html/lsr/products/SKUs 161-4013.html /webroot/web/html/lsr/products/SKUs/161-4013.html Bead size 2903 2.4–3.4 µm 1 0 Bead magnetic content 2954 14–28% 1 0 Typical IgG binding capacity 2289 ≥6 µg/mg 1 0 Solid content in slurry 2953 1.0–1.2% 1 0 Protein A/G content 2952 9–12 µg/mg 1 0 Dispersion medium 2951 TBS + 0.05% Tween 20 + 0.09% sodium azide 1 0 Shelf life 2149 Store for 24 months at 4°C until expiration date 1 0 Number of applications 1867 1 ml vial, 10 assays; 3 ml vial, 30 assays 1 0 1614811 /templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Magnetic-Beads-Racks-Immunoprecipitation/161-4811_1401321571.xml 1614833 /templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Magnetic-Beads-Racks-Immunoprecipitation/161-4833_1401321571.xml 1614813 /templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Magnetic-Beads-Racks-Immunoprecipitation/161-4813_1401321571.xml false $190.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1614013&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1614013&CartQuote=QT&quantity= 1136 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1614013&AddToDefaultWishList=true&quantity= 41105500 190.00 Product detail page 1614013

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SureBeads™ Starter Kit Protein A and G

1614833
Starter kit for immunoprecipitation with protein A and G conjugated magnetic beads; 3 ml protein G bead suspension, 3 ml protein A bead suspension (10 mg beads/ml suspension), and 16-tube magnetic rack

1614833 /templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Magnetic-Beads-Racks-Immunoprecipitation/161-4833_1401321571.xml 1614833 /default/main/bio-rad/WORKAREA/default 05/28/14 04:54 PM en_US jcjsxz2m 2017-11-14 07:08:58 true true internet/product_SKU jcjsxz2m templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Magnetic-Beads-Racks-Immunoprecipitation/161-4833_1401321571.xml LSR 2019-05-04 16:23:42 /default/main/bio-rad/STAGING N /default/main/bio-rad en 05/28/24 04:58 PM 1556043807973 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 16:23:42 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Magnetic-Beads-Racks-Immunoprecipitation/161-4833_1401321571.xml templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Magnetic-Beads-Racks-Immunoprecipitation/161-4833_1401321571.xml 1614833-surebeads-starter-kit-protein-g 1614833 SureBeadsImmunoprecipitationStarterKitAG SureBeads™ Starter Kit Protein A and G Starter kit for immunoprecipitation with protein A and G conjugated magnetic beads; 3 ml protein G bead suspension, 3 ml protein A bead suspension (10 mg beads/ml suspension), and 16-tube magnetic rack /webroot/web/images/lsr/products/protein_sample_preparation/sku_thumb/global/surebeads-immunoprecipitation-starter-kit-a-g-161-4833-thumb.jpg /webroot/web/images/lsr/products/protein_sample_preparation/sku_view/global/surebeads-immunoprecipitation-starter-kit-a-g-161-4833-detail.jpg preview ,VN tommy a SureBeads Immunoprecipitation Starter Kit A/G #161-4833 | Life Science Research | Bio-Rad surebeads, magnetic beads, immunoprecipitation, protein isolation, protein a, protein g, protein a beads, protein g beads 05/28/14 04:54 PM 05/28/24 04:58 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Protein-Sample-Preparation/Magnetic-Beads-Racks-Immunoprecipitation N 0 <p>SureBeads Starter Kits provide an ideal introduction to fast, easy, and reliable immunoprecipitation using protein A and protein G conjugated magnetic beads. The lightweight, ergonomically designed SureBeads Magnetic Rack with separable magnets enables bioseparations in seconds without centrifugation.</p> <h2 class="descHeader">Kit contents:</h2> <ul> <li>3 ml vial SureBeads Protein A Magnetic Beads; 30 mg (10 mg/ml)</li> <li>3 ml vial SureBeads Protein G Magnetic Beads; 30 mg (10 mg/ml)</li> <li>Magnetic rack with one-piece removable magnet insert; holds 1.5 ml and 15 ml tubes <ul> <li>Up to 16 x 1.5 ml microcentrifuge tubes</li> <li>2 x 15 ml conical centrifuge tubes</li> </ul> </li> </ul> <p>Use SureBeads Magnetic Beads for immunoprecipitation (IP), co-immunoprecipitation (co-IP), and protein pull-down assays. Superparamagnetic beads with a surface-activated hydrophilic polymer and chemically conjugated protein A or protein G bind the Fc regions of immunoglobulins with high specificity.</p> <ul> <li>Faster IP using magnetization instead of centrifugation</li> <li>Unique bead surface chemistry ensures optimal antibody orientation for increased antigen binding efficiency</li> <li>High IgG binding affinity with low nonspecific binding and reduced antibody consumption</li> <li>Beads cling magnetically to the side wall of the tube, not the bottom, for easier wash pipetting</li> </ul> <h2 class="descHeader">Packaging Options</h2> <h2> <table class="pd_table" style="margin: 0 0 5px 10px; border-bottom: 1px solid #000000;" border="0"> <tbody> <tr> <td style="border-top: 1px solid #000000;"><strong>Conjugated Protein</strong></td> <td style="border-top: 1px solid #000000;" align="center"><strong>Bead Suspension Volume</strong></td> <td style="border-top: 1px solid #000000;" align="center"><strong>Rack Size</strong><br /> (Number of Tubes)</td> <td style="border-top: 1px solid #000000;" align="center"><strong>Catalog #</strong></td> </tr> <tr class="pd_colorbackground"> <td style="border-top: 1px solid #000000;" valign="top">G</td> <td style="border-top: 1px solid #000000;" align="center" valign="top">1 ml</td> <td style="border-top: 1px solid #000000;" align="center" valign="top">—</td> <td style="border-top: 1px solid #000000;" align="center" valign="top"><a style="font-size: 11px" href="/en-us/sku/1614021-surebeads-protein-g-magnetic-beads-1-ml?ID=1614021">161-4021</a></td> </tr> <tr> <td valign="top">G</td> <td align="center" valign="top">3 ml</td> <td align="center" valign="top">—</td> <td align="center" valign="top"><a style="font-size: 11px" href="/en-us/sku/1614023-surebeads-protein-g-magnetic-beads-3-ml?ID=1614023">161-4023</a></td> </tr> <tr class="pd_colorbackground"> <td valign="top">G</td> <td align="center" valign="top">1 ml</td> <td align="center" valign="top">4*</td> <td align="center" valign="top"><a style="font-size: 11px" href="/en-us/sku/1614821-surebeads-protein-g-magnetic-beads-trial-kit?ID=1614821">161-4821</a></td> </tr> <tr> <td valign="top">A</td> <td align="center" valign="top">1 ml</td> <td align="center" valign="top">—</td> <td align="center" valign="top"><a style="font-size: 11px" href="/en-us/sku/1614011-surebeads-protein-magnetic-beads-1-ml?ID=1614011">161-4011</a></td> </tr> <tr class="pd_colorbackground"> <td valign="top">A</td> <td align="center" valign="top">3 ml</td> <td align="center" valign="top">—</td> <td align="center" valign="top"><a style="font-size: 11px" href="/en-us/sku/1614013-surebeads-protein-magnetic-beads-3-ml?ID=1614013">161-4013</a></td> </tr> <tr> <td valign="top">A</td> <td align="center" valign="top">1 ml</td> <td align="center" valign="top">4*</td> <td align="center" valign="top"><a style="font-size: 11px" href="/en-us/sku/1614811-surebeads-protein-magnetic-beads-trial-kit?ID=1614811">161-4811</a></td> </tr> <tr class="pd_colorbackground"> <td valign="top">A/G</td> <td align="center" valign="top">2 x 3 ml</td> <td align="center" valign="top">16</td> <td align="center" valign="top">161-4833</td> </tr> <tr> <td valign="top">G/G</td> <td align="center" valign="top">2 x 3 ml</td> <td align="center" valign="top">16</td> <td align="center" valign="top"><a style="font-size: 11px" href="/en-us/sku/1614823-surebeads-immunoprecipitation-starter-kit-g-g?ID=1614823">161-4823</a></td> </tr> <tr class="pd_colorbackground"> <td valign="top">A/A</td> <td align="center" valign="top">2 x 3 ml</td> <td align="center" valign="top">16</td> <td align="center" valign="top"><a style="font-size: 11px" href="/en-us/sku/1614813-surebeads-immunoprecipitation-starter-kit?ID=1614813">161-4813</a></td> </tr> <tr> <td colspan="2" valign="top">16-tube magnetic rack</td> <td align="center" valign="top">16</td> <td align="center" valign="top"><a style="font-size: 11px" href="/en-us/sku/1614916-16-tube-surebeads-magnetic-rack?ID=1614916">161-4916</a></td> </tr> </tbody> </table> <p class="caption">*Trial kit</p> </h2> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/category/protein-electrophoresis-blotting">Protein Electrophoresis and Western Blotting</a></li> <li><a href="/en-us/category/protein-sample-preparation">Protein Sample Preparation</a></li> </ul> <h2 class="descHeader">More Information</h2> <p>Protein A and protein G have different binding affinities for various species and subclasses of monoclonal and polyclonal antibodies. Use the table below to select the best SureBeads products for your needs.</p> <h2 class="descHeader" style="clear: both">Protein A/G Immunoprecipitation Guide</h2> <table class="pd_table" style="margin: 0 0 5px 10px; border-bottom: 1px solid #000000;" border="0"> <tbody> <tr> <td style="border-top: 1px solid #000000;" valign="top"><strong>Monoclonal</strong></td> <td style="border-top: 1px solid #000000;" align="center" valign="top"><strong>Protein A</strong></td> <td style="border-top: 1px solid #000000;" align="center" valign="top"><strong>Protein G</strong></td> </tr> <tr class="pd_colorbackground"> <td style="border-top: 1px solid #000000;" valign="top">Human IgG<sub>1</sub></td> <td style="border-top: 1px solid #000000;" align="center" valign="top">+++</td> <td style="border-top: 1px solid #000000;" align="center" valign="top">+++</td> </tr> <tr> <td valign="top">Human IgG<sub>2</sub></td> <td align="center" valign="top">+++</td> <td align="center" valign="top">+++</td> </tr> <tr class="pd_colorbackground"> <td valign="top">Human IgG<sub>3</sub></td> <td align="center" valign="top">–</td> <td align="center" valign="top">+++</td> </tr> <tr> <td valign="top">Human IgG<sub>4</sub></td> <td align="center" valign="top">+++</td> <td align="center" valign="top">+++</td> </tr> <tr class="pd_colorbackground"> <td valign="top">Mouse IgG<sub>1</sub></td> <td align="center" valign="top">+</td> <td align="center" valign="top">+++</td> </tr> <tr> <td valign="top">Mouse IgG<sub>2a</sub></td> <td align="center" valign="top">+++</td> <td align="center" valign="top">+++</td> </tr> <tr class="pd_colorbackground"> <td valign="top">Mouse IgG<sub>2b</sub></td> <td align="center" valign="top">++</td> <td align="center" valign="top">++</td> </tr> <tr> <td valign="top">Mouse IgG<sub>3</sub></td> <td align="center" valign="top">++</td> <td align="center" valign="top">++</td> </tr> <tr class="pd_colorbackground"> <td valign="top">Rat IgG<sub>1</sub></td> <td align="center" valign="top">–</td> <td align="center" valign="top">+</td> </tr> <tr> <td valign="top">Rat IgG<sub>2a</sub>a</td> <td align="center" valign="top">–</td> <td align="center" valign="top">+++</td> </tr> <tr class="pd_colorbackground"> <td valign="top">Rat IgG<sub>2b</sub></td> <td align="center" valign="top">–</td> <td align="center" valign="top">++</td> </tr> <tr> <td valign="top">Rat IgG<sub>2c</sub></td> <td align="center" valign="top">+</td> <td align="center" valign="top">++</td> </tr> <tr> <td style="border-top: 1px solid #000000; border-bottom: 1px solid #000000;" valign="top"><strong>Polyclonal</strong></td> <td style="border-top: 1px solid #000000; border-bottom: 1px solid #000000;" align="center" valign="top"><strong>Protein A</strong></td> <td style="border-top: 1px solid #000000; border-bottom: 1px solid #000000;" align="center" valign="top"><strong>Protein G</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">Rabbit</td> <td align="center" valign="top">+++</td> <td align="center" valign="top">++</td> </tr> <tr> <td valign="top">Cow</td> <td align="center" valign="top">++</td> <td align="center" valign="top">+++</td> </tr> <tr class="pd_colorbackground"> <td valign="top">Horse</td> <td align="center" valign="top">++</td> <td align="center" valign="top">+++</td> </tr> <tr> <td valign="top">Goat</td> <td align="center" valign="top">–</td> <td align="center" valign="top">++</td> </tr> <tr class="pd_colorbackground"> <td valign="top">Guinea pig</td> <td align="center" valign="top">+++</td> <td align="center" valign="top">++</td> </tr> <tr> <td valign="top">Sheep</td> <td align="center" valign="top">+/-</td> <td align="center" valign="top">++</td> </tr> <tr class="pd_colorbackground"> <td valign="top">Pig</td> <td align="center" valign="top">++</td> <td align="center" valign="top">++</td> </tr> <tr> <td valign="top">Rat</td> <td align="center" valign="top">+/-</td> <td align="center" valign="top">++</td> </tr> <tr class="pd_colorbackground"> <td valign="top">Mouse</td> <td align="center" valign="top">++</td> <td align="center" valign="top">++</td> </tr> <tr> <td valign="top">Chicken</td> <td align="center" valign="top">–</td> <td align="center" valign="top">+</td> </tr> <tr class="pd_colorbackground"> <td valign="top">Human IgG</td> <td align="center" valign="top">+++</td> <td align="center" valign="top">+++</td> </tr> <tr> <td valign="top">Human IgM</td> <td align="center" valign="top">–</td> <td align="center" valign="top">+</td> </tr> <tr class="pd_colorbackground"> <td valign="top">Human IgD</td> <td align="center" valign="top">–</td> <td align="center" valign="top">+</td> </tr> <tr> <td valign="top">Human IgA</td> <td align="center" valign="top">–</td> <td align="center" valign="top">+</td> </tr> </tbody> </table> <p class="caption">– No binding<br />+ Weak binding<br />++ Moderate binding<br />+++ Strong binding</p>  /webroot/web/html/lsr/products/SKUs 161-4833.html /webroot/web/html/lsr/products/SKUs/161-4833.html Bead size 2903 2.4–3.4 µm 1 0 Bead magnetic content 2954 14–28% 1 0 Typical IgG binding capacity 2289 ≥6 µg/mg 1 0 Solid content in slurry 2953 1.0–1.2% 1 0 Protein A/G content 2952 9–12 µg/mg 1 0 Dispersion medium 2951 TBS + 0.05% Tween 20 + 0.09% sodium azide 1 0 Shelf life 2149 Store for 24 months at 4°C until expiration date 1 0 Number of applications 1867 1 ml vial, 10 assays; 3 ml vial, 30 assays 1 0 1614821 /templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Magnetic-Beads-Racks-Immunoprecipitation/161-4821_1401321571.xml 1614021 /templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Magnetic-Beads-Racks-Immunoprecipitation/161-4021_1401321571.xml 1614823 /templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Magnetic-Beads-Racks-Immunoprecipitation/161-4823_1401321571.xml false $681.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1614833&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1614833&CartQuote=QT&quantity= 1136 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1614833&AddToDefaultWishList=true&quantity= 41105500 681.00 Product detail page 1614833

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SureBeads™ Starter Kit Protein A

1614813
Starter kit for immunoprecipitation with protein A conjugated magnetic beads, contains 2 x 3 ml protein A beads suspension (10 mg beads/ml suspension) and a 16-tube magnetic rack

This product is being discontinued soon. Please contact customer service for more information.

SureBeads™ Protein A Magnetic Beads, 1 ml

1614011
1 ml, suspension of protein A conjugated magnetic immunoprecipitation beads (10 mg beads/ml)

1614011 /templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Magnetic-Beads-Racks-Immunoprecipitation/161-4011_1401321571.xml 1614011 /default/main/bio-rad/WORKAREA/default 05/28/14 04:54 PM en_US jcjsxz4h 2017-11-14 07:08:58 true true internet/product_SKU jcjsxz4h templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Magnetic-Beads-Racks-Immunoprecipitation/161-4011_1401321571.xml LSR 2019-05-04 16:23:49 /default/main/bio-rad/STAGING N /default/main/bio-rad en 05/28/24 04:58 PM 1556043807388 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 16:23:49 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Magnetic-Beads-Racks-Immunoprecipitation/161-4011_1401321571.xml templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Magnetic-Beads-Racks-Immunoprecipitation/161-4011_1401321571.xml 1614011-surebeads-protein-magnetic-beads-1-ml 1614011 SureBeadsProteinAMagneticBeads1ml SureBeads™ Protein A Magnetic Beads, 1 ml 1 ml, suspension of protein A conjugated magnetic immunoprecipitation beads (10 mg beads/ml) /webroot/web/images/lsr/products/protein_sample_preparation/sku_thumb/global/surebeads-protein-a-magnetic-beads-1ml-sku-161-4011-thumb.jpg /webroot/web/images/lsr/products/protein_sample_preparation/sku_view/global/surebeads-protein-a-magnetic-beads-1ml-sku-161-4011-detail.jpg preview ,VN tommy a SureBeads Protein A Magnetic Beads 1 ml #161-4011 | Life Science Research | Bio-Rad surebeads, magnetic beads, immunoprecipitation, protein isolation, protein A 05/28/14 04:54 PM 05/28/24 04:58 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Protein-Sample-Preparation/Magnetic-Beads-Racks-Immunoprecipitation N 0 <p>Use SureBeads Protein A Magnetic Beads for fast, easy, and reliable bioseparations: immunoprecipitation (IP), co-immunoprecipitation (co-IP), and protein pull-down assays.</p> <p>Superparamagnetic beads with surface-activated hydrophilic polymer and chemically conjugated protein A bind the Fc regions of immunoglobulins with high specificity.</p> <ul> <li>Faster IP using magnetization instead of centrifugation</li> <li>Unique bead surface chemistry ensures optimal antibody orientation for increased antigen binding efficiency</li> <li>High IgG binding affinity with low nonspecific binding and reduced antibody consumption</li> <li>Beads cling magnetically to the side wall of the tube, not the bottom, for easier wash pipetting</li> </ul> <h2 class="descHeader">Packaging Options</h2> <h2> <table class="pd_table" style="margin: 0 0 5px 10px; border-bottom: 1px solid #000000;" border="0"> <tbody> <tr> <td style="border-top: 1px solid #000000;"><strong>Conjugated Protein</strong></td> <td style="border-top: 1px solid #000000;" align="center"><strong>Bead Suspension Volume</strong></td> <td style="border-top: 1px solid #000000;" align="center"><strong>Rack Size</strong><br /> (Number of Tubes)</td> <td style="border-top: 1px solid #000000;" align="center"><strong>Catalog #</strong></td> </tr> <tr class="pd_colorbackground"> <td style="border-top: 1px solid #000000;" valign="top">G</td> <td style="border-top: 1px solid #000000;" align="center" valign="top">1 ml</td> <td style="border-top: 1px solid #000000;" align="center" valign="top">—</td> <td style="border-top: 1px solid #000000;" align="center" valign="top"><a style="font-size: 11px" href="/en-us/sku/1614021-surebeads-protein-g-magnetic-beads-1-ml?ID=1614021">161-4021</a></td> </tr> <tr> <td valign="top">G</td> <td align="center" valign="top">3 ml</td> <td align="center" valign="top">—</td> <td align="center" valign="top"><a style="font-size: 11px" href="/en-us/sku/1614023-surebeads-protein-g-magnetic-beads-3-ml?ID=1614023">161-4023</a></td> </tr> <tr class="pd_colorbackground"> <td valign="top">G</td> <td align="center" valign="top">1 ml</td> <td align="center" valign="top">4*</td> <td align="center" valign="top"><a style="font-size: 11px" href="/en-us/sku/1614821-surebeads-protein-g-magnetic-beads-trial-kit?ID=1614821">161-4821</a></td> </tr> <tr> <td valign="top">A</td> <td align="center" valign="top">1 ml</td> <td align="center" valign="top">—</td> <td align="center" valign="top">161-4011</td> </tr> <tr class="pd_colorbackground"> <td valign="top">A</td> <td align="center" valign="top">3 ml</td> <td align="center" valign="top">—</td> <td align="center" valign="top"><a style="font-size: 11px" href="/en-us/sku/1614013-surebeads-protein-magnetic-beads-3-ml?ID=1614013">161-4013</a></td> </tr> <tr> <td valign="top">A</td> <td align="center" valign="top">1 ml</td> <td align="center" valign="top">4*</td> <td align="center" valign="top"><a style="font-size: 11px" href="/en-us/sku/1614811-surebeads-protein-magnetic-beads-trial-kit?ID=1614811">161-4811</a></td> </tr> <tr class="pd_colorbackground"> <td valign="top">A/G</td> <td align="center" valign="top">2 x 3 ml</td> <td align="center" valign="top">16</td> <td align="center" valign="top"><a style="font-size: 11px" href="/en-us/sku/1614833-surebeads-immunoprecipitation-starter-kit-g?ID=1614833">161-4833</a></td> </tr> <tr> <td valign="top">G/G</td> <td align="center" valign="top">2 x 3 ml</td> <td align="center" valign="top">16</td> <td align="center" valign="top"><a style="font-size: 11px" href="/en-us/sku/1614823-surebeads-immunoprecipitation-starter-kit-g-g?ID=1614823">161-4823</a></td> </tr> <tr class="pd_colorbackground"> <td valign="top">A/A</td> <td align="center" valign="top">2 x 3 ml</td> <td align="center" valign="top">16</td> <td align="center" valign="top"><a style="font-size: 11px" href="/en-us/sku/1614813-surebeads-immunoprecipitation-starter-kit?ID=1614813">161-4813</a></td> </tr> <tr> <td colspan="2" valign="top">16-tube magnetic rack</td> <td align="center" valign="top">16</td> <td align="center" valign="top"><a style="font-size: 11px" href="/en-us/sku/1614916-16-tube-surebeads-magnetic-rack?ID=1614916">161-4916</a></td> </tr> </tbody> </table> <p class="caption">*Trial kit</p> </h2> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/category/protein-electrophoresis-blotting">Protein Electrophoresis and Western Blotting</a></li> <li><a href="/en-us/category/protein-sample-preparation">Protein Sample Preparation</a></li> </ul> <h2 class="descHeader">More Information</h2> <p>Protein A and protein G have different binding affinities for various species and subclasses of monoclonal and polyclonal antibodies. Use the table below to select the best SureBeads products for your needs.</p> <h2 class="descHeader" style="clear: both">Protein A/G Immunoprecipitation Guide</h2> <table class="pd_table" style="margin: 0 0 5px 10px; border-bottom: 1px solid #000000;" border="0"> <tbody> <tr> <td style="border-top: 1px solid #000000;" valign="top"><strong>Monoclonal</strong></td> <td style="border-top: 1px solid #000000;" align="center" valign="top"><strong>Protein A</strong></td> <td style="border-top: 1px solid #000000;" align="center" valign="top"><strong>Protein G</strong></td> </tr> <tr class="pd_colorbackground"> <td style="border-top: 1px solid #000000;" valign="top">Human IgG<sub>1</sub></td> <td style="border-top: 1px solid #000000;" align="center" valign="top">+++</td> <td style="border-top: 1px solid #000000;" align="center" valign="top">+++</td> </tr> <tr> <td valign="top">Human IgG<sub>2</sub></td> <td align="center" valign="top">+++</td> <td align="center" valign="top">+++</td> </tr> <tr class="pd_colorbackground"> <td valign="top">Human IgG<sub>3</sub></td> <td align="center" valign="top">–</td> <td align="center" valign="top">+++</td> </tr> <tr> <td valign="top">Human IgG<sub>4</sub></td> <td align="center" valign="top">+++</td> <td align="center" valign="top">+++</td> </tr> <tr class="pd_colorbackground"> <td valign="top">Mouse IgG<sub>1</sub></td> <td align="center" valign="top">+</td> <td align="center" valign="top">+++</td> </tr> <tr> <td valign="top">Mouse IgG<sub>2a</sub></td> <td align="center" valign="top">+++</td> <td align="center" valign="top">+++</td> </tr> <tr class="pd_colorbackground"> <td valign="top">Mouse IgG<sub>2b</sub></td> <td align="center" valign="top">++</td> <td align="center" valign="top">++</td> </tr> <tr> <td valign="top">Mouse IgG<sub>3</sub></td> <td align="center" valign="top">++</td> <td align="center" valign="top">++</td> </tr> <tr class="pd_colorbackground"> <td valign="top">Rat IgG<sub>1</sub></td> <td align="center" valign="top">–</td> <td align="center" valign="top">+</td> </tr> <tr> <td valign="top">Rat IgG<sub>2a</sub>a</td> <td align="center" valign="top">–</td> <td align="center" valign="top">+++</td> </tr> <tr class="pd_colorbackground"> <td valign="top">Rat IgG<sub>2b</sub></td> <td align="center" valign="top">–</td> <td align="center" valign="top">++</td> </tr> <tr> <td valign="top">Rat IgG<sub>2c</sub></td> <td align="center" valign="top">+</td> <td align="center" valign="top">++</td> </tr> <tr> <td style="border-top: 1px solid #000000; border-bottom: 1px solid #000000;" valign="top"><strong>Polyclonal</strong></td> <td style="border-top: 1px solid #000000; border-bottom: 1px solid #000000;" align="center" valign="top"><strong>Protein A</strong></td> <td style="border-top: 1px solid #000000; border-bottom: 1px solid #000000;" align="center" valign="top"><strong>Protein G</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">Rabbit</td> <td align="center" valign="top">+++</td> <td align="center" valign="top">++</td> </tr> <tr> <td valign="top">Cow</td> <td align="center" valign="top">++</td> <td align="center" valign="top">+++</td> </tr> <tr class="pd_colorbackground"> <td valign="top">Horse</td> <td align="center" valign="top">++</td> <td align="center" valign="top">+++</td> </tr> <tr> <td valign="top">Goat</td> <td align="center" valign="top">–</td> <td align="center" valign="top">++</td> </tr> <tr class="pd_colorbackground"> <td valign="top">Guinea pig</td> <td align="center" valign="top">+++</td> <td align="center" valign="top">++</td> </tr> <tr> <td valign="top">Sheep</td> <td align="center" valign="top">+/-</td> <td align="center" valign="top">++</td> </tr> <tr class="pd_colorbackground"> <td valign="top">Pig</td> <td align="center" valign="top">++</td> <td align="center" valign="top">++</td> </tr> <tr> <td valign="top">Rat</td> <td align="center" valign="top">+/-</td> <td align="center" valign="top">++</td> </tr> <tr class="pd_colorbackground"> <td valign="top">Mouse</td> <td align="center" valign="top">++</td> <td align="center" valign="top">++</td> </tr> <tr> <td valign="top">Chicken</td> <td align="center" valign="top">–</td> <td align="center" valign="top">+</td> </tr> <tr class="pd_colorbackground"> <td valign="top">Human IgG</td> <td align="center" valign="top">+++</td> <td align="center" valign="top">+++</td> </tr> <tr> <td valign="top">Human IgM</td> <td align="center" valign="top">–</td> <td align="center" valign="top">+</td> </tr> <tr class="pd_colorbackground"> <td valign="top">Human IgD</td> <td align="center" valign="top">–</td> <td align="center" valign="top">+</td> </tr> <tr> <td valign="top">Human IgA</td> <td align="center" valign="top">–</td> <td align="center" valign="top">+</td> </tr> </tbody> </table> <p class="caption">– No binding<br />+ Weak binding<br />++ Moderate binding<br />+++ Strong binding</p>  /webroot/web/html/lsr/products/SKUs 161-4011.html /webroot/web/html/lsr/products/SKUs/161-4011.html Bead size 2903 2.4–3.4 µm 1 0 Bead magnetic content 2954 14–28% 1 0 Typical IgG binding capacity 2289 > 6 µg/mg 1 0 Solid content in slurry 2953 1.0–1.2% 1 0 Protein A/G content 2952 9–12 µg/mg 1 0 Dispersion medium 2951 TBS + 0.05% Tween 20 + 0.09% sodium azide 1 0 Shelf life 2149 Store for 24 months at 4°C until expiration date 1 0 Number of applications 1867 1 ml vial, 10 assays; 3 ml vial, 30 assays 1 0 1614811 /templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Magnetic-Beads-Racks-Immunoprecipitation/161-4811_1401321571.xml 1614833 /templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Magnetic-Beads-Racks-Immunoprecipitation/161-4833_1401321571.xml 1614813 /templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Magnetic-Beads-Racks-Immunoprecipitation/161-4813_1401321571.xml false $103.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1614011&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1614011&CartQuote=QT&quantity= 1136 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1614011&AddToDefaultWishList=true&quantity= 41105500 103.00 Product detail page 1614011

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16-Tube SureBeads™ Magnetic Rack

1614916
Rack with high-strength magnets for rapid magnetic bead separation. Supports 16 x 1.5 ml microcentrifuge tubes or 2 x 15 ml conical tubes. Use with SureBeads Magnetic Beads.

1614916 /templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Magnetic-Beads-Racks-Immunoprecipitation/161-4916_1401321571.xml 1614916 /default/main/bio-rad/WORKAREA/default 05/28/14 04:54 PM en_US jcjsxz21 2017-11-14 07:08:58 true true internet/product_SKU jcjsxz21 templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Magnetic-Beads-Racks-Immunoprecipitation/161-4916_1401321571.xml LSR 2019-05-04 16:23:40 /default/main/bio-rad/STAGING N /default/main/bio-rad en 05/28/24 04:58 PM 1556043808020 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 16:23:40 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Magnetic-Beads-Racks-Immunoprecipitation/161-4916_1401321571.xml templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Magnetic-Beads-Racks-Immunoprecipitation/161-4916_1401321571.xml 1614916-16-tube-surebeads-magnetic-rack 1614916 16-TubeMagneticRackforSureBeadsImmunoprecipitationBeads 16-Tube SureBeads™ Magnetic Rack Rack with high-strength magnets for rapid magnetic bead separation. Supports 16 x 1.5 ml microcentrifuge tubes or 2 x 15 ml conical tubes. Use with SureBeads Magnetic Beads. /webroot/web/images/lsr/products/protein_sample_preparation/sku_thumb/global/16-tube-magnetic-rack-surebeads-immunoprecipitation-beads-sku-161-4916-thumb.jpg /webroot/web/images/lsr/products/protein_sample_preparation/sku_view/global/16-tube-magnetic-rack-surebeads-immunoprecipitation-beads-sku-161-4916-detail.jpg preview ,VN tommy a SureBeads Magnetic Rack, 16 tube holder #161-4916 | Life Science Research | Bio-Rad Rack with high-strength magnets for rapid magnetic bead separation. Supports 16 x 1.5 ml microcentrifuge tubes or 2 x 15 ml conical tubes. Use with SureBeads Magnetic Beads. surebeads, magnetic beads, immunoprecipitation, protein isolation, protein a, protein g, protein a beads, protein g beads, magnetic rack 05/28/14 04:54 PM 05/28/24 04:58 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Protein-Sample-Preparation/Magnetic-Beads-Racks-Immunoprecipitation N 0 <p>Use this ergonomically designed tube rack with high-strength magnets to separate SureBeads Magnetic Beads from the supernatant in seconds, allowing for easy pipet removal without sample loss. Resuspend samples rapidly by removing the magnet cassette and vortexing all the tubes at once while still in the rack.</p> <ul> <li>Lightweight, easy-to-hold rack with one-piece removable magnet insert</li> <li>Holds 1.5 ml and 15 ml tubes <ul> <li>Up to 16 x 1.5 ml microcentrifuge tubes</li> <li>2 x 15 ml conical centrifuge tubes</li> </ul> </li> </ul> <p style="margin: 0px 0px 0px 10px; text-align: left;"><iframe src="https://www.youtube.com/embed/aHsBID5oS4k?version=3&rel=0&showinfo=0&theme=light&modestbranding=1&fs=1;wmode=transparent" width="350" height="197"></iframe><br /></p> <p class="caption" style="clear:both; margin: 0px 0px 0px 10px;">See the SureBeads Magnetic Rack in action.</p> <p>SureBeads Magnetic Beads enable fast, easy, and reliable bioseparations: immunoprecipitation (IP), co-immunoprecipitation (co-IP), and protein pull-down assays. Superparamagnetic beads with a surface-activated hydrophilic polymer and chemically conjugated protein A or protein G bind the Fc regions of immunoglobulins with high specificity.</p> <ul> <li>Faster IP using magnetization instead of centrifugation</li> <li>Unique bead surface chemistry ensures optimal antibody orientation for increased antigen binding efficiency</li> <li>High IgG binding affinity with low nonspecific binding and reduced antibody consumption</li> <li>Beads cling magnetically to the side wall of the tube, not the bottom, for easier wash pipetting</li> </ul> <h2 class="descHeader">Packaging Options</h2> <h2> <table class="pd_table" style="margin: 0 0 5px 10px; border-bottom: 1px solid #000000;" border="0"> <tbody> <tr> <td style="border-top: 1px solid #000000;"><strong>Conjugated Protein</strong></td> <td style="border-top: 1px solid #000000;" align="center"><strong>Bead Suspension Volume</strong></td> <td style="border-top: 1px solid #000000;" align="center"><strong>Rack Size</strong><br /> (Number of Tubes)</td> <td style="border-top: 1px solid #000000;" align="center"><strong>Catalog #</strong></td> </tr> <tr class="pd_colorbackground"> <td style="border-top: 1px solid #000000;" valign="top">G</td> <td style="border-top: 1px solid #000000;" align="center" valign="top">1 ml</td> <td style="border-top: 1px solid #000000;" align="center" valign="top">—</td> <td style="border-top: 1px solid #000000;" align="center" valign="top"><a style="font-size: 11px" href="/en-us/sku/1614021-surebeads-protein-g-magnetic-beads-1-ml?ID=1614021">161-4021</a></td> </tr> <tr> <td valign="top">G</td> <td align="center" valign="top">3 ml</td> <td align="center" valign="top">—</td> <td align="center" valign="top"><a style="font-size: 11px" href="/en-us/sku/1614023-surebeads-protein-g-magnetic-beads-3-ml?ID=1614023">161-4023</a></td> </tr> <tr class="pd_colorbackground"> <td valign="top">G</td> <td align="center" valign="top">1 ml</td> <td align="center" valign="top">4*</td> <td align="center" valign="top"><a style="font-size: 11px" href="/en-us/sku/1614821-surebeads-protein-g-magnetic-beads-trial-kit?ID=1614821">161-4821</a></td> </tr> <tr> <td valign="top">A</td> <td align="center" valign="top">1 ml</td> <td align="center" valign="top">—</td> <td align="center" valign="top"><a style="font-size: 11px" href="/en-us/sku/1614011-surebeads-protein-magnetic-beads-1-ml?ID=1614011">161-4011</a></td> </tr> <tr class="pd_colorbackground"> <td valign="top">A</td> <td align="center" valign="top">3 ml</td> <td align="center" valign="top">—</td> <td align="center" valign="top"><a style="font-size: 11px" href="/en-us/sku/1614013-surebeads-protein-magnetic-beads-3-ml?ID=1614013">161-4013</a></td> </tr> <tr> <td valign="top">A</td> <td align="center" valign="top">1 ml</td> <td align="center" valign="top">4*</td> <td align="center" valign="top"><a style="font-size: 11px" href="/en-us/sku/1614811-surebeads-protein-magnetic-beads-trial-kit?ID=1614811">161-4811</a></td> </tr> <tr class="pd_colorbackground"> <td valign="top">A/G</td> <td align="center" valign="top">2 x 3 ml</td> <td align="center" valign="top">16</td> <td align="center" valign="top"><a style="font-size: 11px" href="/en-us/sku/1614833-surebeads-immunoprecipitation-starter-kit-g?ID=1614833">161-4833</a></td> </tr> <tr> <td valign="top">G/G</td> <td align="center" valign="top">2 x 3 ml</td> <td align="center" valign="top">16</td> <td align="center" valign="top"><a style="font-size: 11px" href="/en-us/sku/1614823-surebeads-immunoprecipitation-starter-kit-g-g?ID=1614823">161-4823</a></td> </tr> <tr class="pd_colorbackground"> <td valign="top">A/A</td> <td align="center" valign="top">2 x 3 ml</td> <td align="center" valign="top">16</td> <td align="center" valign="top"><a style="font-size: 11px" href="/en-us/sku/1614813-surebeads-immunoprecipitation-starter-kit?ID=1614813">161-4813</a></td> </tr> <tr> <td colspan="2" valign="top">16-tube magnetic rack</td> <td align="center" valign="top">16</td> <td align="center" valign="top">161-4916</td> </tr> </tbody> </table> <p class="caption">*Trial kit</p> </h2> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/category/protein-electrophoresis-blotting">Protein Electrophoresis and Western Blotting</a></li> <li><a href="/en-us/category/protein-sample-preparation">Protein Sample Preparation</a></li> </ul> /webroot/web/html/lsr/products/SKUs 161-4916.html /webroot/web/html/lsr/products/SKUs/161-4916.html <strong>Sample capacity</strong> 2965   1 0 1.5 ml microcentrifuge tubes 2499 16 1 0 15 ml conical tubes 2500 2 1 0 1614833 /templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Magnetic-Beads-Racks-Immunoprecipitation/161-4833_1401321571.xml 1614813 /templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Magnetic-Beads-Racks-Immunoprecipitation/161-4813_1401321571.xml 1614823 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Documents

Number	Description	Options
7118	Six Tips to Improve Your Co-IP Results, Ver A	Click to download	7118 [ Add to Cart (Free) ] 0
7110	Chromatin Immunoprecipitation (ChIP) with SureBeads Protein A and G Magnetic Beads	Click to download	7110 [ Add to Cart (Free) ] 0
6560	SureBeads™ Magnetic Beads Competitive Sales Flyer	Click to download	6560 [ Add to Cart (Free) ] 0
6594	Immunoprecipitation Performance of SureBeads™ Protein A Magnetic Beads vs. Protein A Agarose Beads, Rev A	Click to download
10041689	SureBeads™ Magnetic Beads for Immunoprecipitation Product Insert, Rev A	Click to download
10043651	SureBeads™ Magnetic Beads Standard Immunoprecipitation Protocol, Rev A	Click to download
7087	Protein Sample Preparation Publications List	Click to download

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SureBeads™ Protein A Magnetic Beads

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Immunoprecipitation in five easy steps:

Features and Benefits of SureBeads Protein A Magnetic Beads

Applications and Uses of SureBeads Protein A Magnetic Beads

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Protein A/G Immunoprecipitation Guide

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SureBeads™ Trial Kit Protein A

SureBeads™ Protein A Magnetic Beads, 3 ml

SureBeads™ Starter Kit Protein A and G

SureBeads™ Starter Kit Protein A

SureBeads™ Protein A Magnetic Beads, 1 ml

16-Tube SureBeads™ Magnetic Rack

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