SEQuoia Express Stranded RNA Library Prep Kit is a fast, high-performance RNA-Seq library preparation kit used to construct a sequence-ready library comprised of messenger RNA and long-noncoding RNA biotypes that is compatible with Illumina platforms from a broad range of total RNA inputs. Featuring SEQzyme, a proprietary engineered enzyme that couples cDNA synthesis with adapter addition in a continuous synthesis reaction, this kit streamlines library construction by reducing the number of components to just three tubes, significantly minimizing the number of pipetting steps and shortening the protocol time to under three hours.
SEQuoia Express Stranded RNA Library Prep Kit is compatible with SEQuoia RiboDepletion Kit for removal of constructs originating from ribosomal RNA and SEQuoia Dual-Indexed Primers, enabling a user to pool libraries and run them on a single flow-cell, greatly reducing the cost of over sequencing. Furthermore, it comes with a pre-packaged bioinformatics pipeline with the options to select a researcher’s local infrastructure or the cloud-based web application. This rapid and cost-effective workflow provides a seamless end-to-end solution for researchers who want to generate the highest quality data with minimal chance of error.
Features and Benefits
- Simplified workflow that can be completed under 3 hours
- Convenient 3-tube configuration minimizes pipetting steps and hands-on time
- Efficient and unbiased capture of coding and non-coding long RNA
- Validated with a broad range of RNA input
- Integrated with bioinformatics solution
Applications and Uses
For researchers and drug developers who require high-quality, complex, and unbiased sequencing libraries to profile differential expression patterns of mRNA and long non-coding RNA biotypes and be able to quickly assess the quality of their sequencing data. Main applications include:
- Liquid biopsy
- Single nucleotide variation discovery
- Gene fusion identification
- Splice junction identification
Each kit comes with three reagent components: reaction mix, enzyme mix, and an amplification mix. The kit should be stored at -20°C.
SEQuoia Express Stranded RNA Library Prep Technology FAQs
SEQuoia Express Stranded RNA Library Prep Technology
How does SEQuoia Express retain strand information?
Why is SEQuoia Express more efficient in capturing long RNAs?
How does SEQuoia Express construct library with a shorter workflow?
What is UMI and what is the purpose of having UMI in our adapter design?
What does a typical Bioanalyzer trace of a SEQuoia Express library look like?
SEQuoia Express Stranded RNA Library Prep Kit Information
What is included in the kit?
What is the input range for the kit?
What is the shelf life of the kit?
Do you offer sample indexes work with the kit?
Do you offer a ribosomal RNA depletion product to work with this library prep kit?
SEQuoia Express Stranded RNA Library Prep Data Analysis
Do you offer a data analysis pipeline to work with SEQuoia Express Stranded RNA Library Prep Kit?
What is the difference between the web hosted solution and the toolkit?
Do I require to use SeqSense analysis pipeline to analyze my sequencing data?
|12017297||SEQuoia Express Stranded RNA Library Prep Kit||24 rxn|
|12017265||SEQuoia Express Stranded RNA Library Prep Kit||96 rxn|
- SEQuoia RiboDepletion Kit
- SEQuoia Dual Indexed Primers, Set
- SEQuoia Dual Indexed Primers, Plate
- CFX Opus Deep Well Real-Time PCR System
- 0.2 ml 8-Tube PCR Strips and Domed Cap Strips
- 0.2 ml 12-Tube PCR Strips and Domed Cap Strips
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Pkg of 1, kit for 24 reactions, includes 600 µl SEQuoia Express Reaction Mix, 120 µl SEQuoia Express Enzyme Mix, and 600 µl SEQuoia Express Amplification Mix
Pkg of 1, kit for 96 reactions, includes 2 x 1,200 µl SEQuoia Express Reaction Mix, 480 µl SEQuoia Express Enzyme Mix, and 2 x 1,200 µl SEQuoia Express Amplification Mix