SEQuoia Express Stranded RNA Library Prep Kit

The strandedness is maintained through the unique properties of SEQzyme, a highly engineered, multifunctional, patented retrotransposon. SEQzyme transcription is initiated on the 3’ end, therefore only 3’ ends of RNA will receive the 1st adapter. This highly efficient continuous synthesis reaction results in a sequencing-ready library with over 98% strandedness information preserved.

SEQuoia Express utilizes blunt end attachment of adapters to RNA fragments. The ratio of SPRI beads used for sample clean-up removes these adapter-dimers and other smaller RNA fragments. This results in the capture of mRNA and long non-coding RNAs (>200 bp).

SEQuoia Express is simplified by utilizing blunt end attachment of adapters to RNA fragments. This bypasses the ligation steps that involve many of the cumbersome pipetting and incubation steps required in other library prep kits and allows for a shorter, simpler workflow.

Unique molecular tags (UMI) are molecular tags typically of 6-12 random base pairs that allow researchers to distinguish transcript duplicates that originate from a single molecule. UMI is incorporated in our adapter design so researchers can easily identify PCR duplicates and detect bias that is introduced during the amplification process

Following library construction, assess the library quality on an Agilent Bioanalyzer 2100. The average peak size is approximately 350-450 bp.

100 ng Universal Human Reference RNA

100 ng Human Placenta RNA

The kit provides all reagents required to construct a library from RNA, with the exception of the SPRISelect beads from Beckman Coulter.

The kit is compatible with a broad dynamic range of input material, from 1 ng to 1 µg total RNA sample.

SEQuoia Express Stranded RNA Library Prep kit is guaranteed for 12 month shelf life after the shipping date if the product is stored properly at -20°C.

Yes! We offer two options of Unique Dual Indexed (UDI) Primers. They are pre-blended and balanced to achieve optimal multiplexed sequencing results. They are in 12 UDI or 96 UDI configurations. Visit bio-rad.com/SEQDIP for ordering information.

Yes! We suggest use SEQuoia Express Library Prep Kit with Bio-Rad’s multiplex-capable, post-amplification SEQuoia RiboDepletion Kit. Visit bio-rad.com/SEQuoiaRiboDepletion for ordering information.

Yes! We offer a pre-packaged, customized bioinformatics pipeline, optimized for SEQouia Express data analysis. They come with two options: The analysis toolkit can be downloaded as a Docker container and installed on the researcher’s local infrastructure; Alternatively, a customer can upload their data to a web-based, hosted version of the toolkit.

The difference relates to where the analysis takes place. The web hosted solution is a secure, cloud-based environment that provides an integrated preconfigured data analysis workflow that allows the user to perform initial analyses with only a few mouse clicks. The toolkit is a command line application packaged with required accessories, such as libraries and other dependencies. A user would download this docker container to run the application on their infrastructure.

We strongly recommend customer to use this complementary, customized data analysis software to process your sequencing data generated using SEQuoia library. For non-SeqSense user, you will require trimming of the contigs prior to alignment (guidelines are provided in the manual). In addition, it is likely that you will need to assign a reference library for non-gene RNA transcripts. For convenience, the SeqSense Analysis Software includes both of these functionalities.