Bio-Rad Protein Assay

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Overview

The Bradford protein assay is a simple colorimetric assay for measuring total protein concentration using a dye-binding method based on the Bradford assay.
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Description

Description

The Bio-Rad protein assay is a simple colorimetric assay for measuring total protein concentration and is based on the Bradford dye-binding method (Bradford 1976). Using standard procedure, the assay is used with samples having protein concentrations between 200 and 1,400 µg/ml (20–140 µg total). It is easy to adapt the assay from the standard-concentration range to a low-concentration (<25 µg/ml; 1–20 µg total) microassay or for rapid determinations in 96-well microplates.

The Bio-Rad protein assay is:

  • Based on the color change of Coomassie brilliant blue G-250 dye in response to various concentrations of protein — the dye binds to primarily basic (especially arginine) and aromatic amino acid residues
  • Useful for measuring proteins and polypeptides, depending on the charged groups, with molecular weights >3,000–5,000

Many detergents and basic protein buffers interfere with the assay; interference may be caused by chemical-protein or chemical-dye interactions. Refer to the list of compatible reagents for more information.

Bio-Rad protein assay kits offer either bovine serum albumin or bovine γ-globulin standards.

Reference
Bradford MM (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72. 248–254.

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items

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5000001
Colorimetric assay kit, includes 450 ml dye reagent concentrate, bovine γ-globulin standard; for 440 standard assays or 2,200 microplate assays
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5000002
Colorimetric assay kit, includes 450 ml dye reagent concentrate, bovine serum albumin standard; for 440 standard assays or 2,200 microplate assays
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5000006
450 ml, colorimetric assay dye, concentrate, for use with Bio-Rad Protein Assay Kits I and II (#5000001, 5000002)
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(Discontinued) Protein Standard I
5000005
Pkg of 1 vial, lyophilized bovine γ-globulin; 20 ml (reconstituted)
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(Discontinued) Protein Standard II
5000007
Pkg of 1 vial, lyophilized bovine serum albumin, 20 ml (reconstituted)

Accessories

Accessories

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2239950
Pkg of 100, 3.5 ml cuvette
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2239955
Pkg of 100, 1.5 ml cuvette

Documents

Documents

Number Description Options
1123
Protein Assay Applications Bibliography
LIT33
Instruction Manual, Bio-Rad Protein Assay, Rev C
1069
Colorimetric Protein Assays, Rev D
6852
Reagent Compatibility Chart for Bio-Rad Protein Assays Quick Guide,Ver A