DC Protein Assay

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Overview

The DC (detergent compatible) protein assay uses a colorimetric method to determine protein concentration following detergent solubilization. The reaction is similar to the well-documented Lowry assay.

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Description

Description

The DC (detergent compatible) protein assay is a colorimetric assay for protein concentration following detergent solubilization. The reaction is similar to the well-documented Lowry assay, but has been modified to save time. The DC protein assay requires only a single 15 minute incubation, and absorbance is stable for at least 2 hours.

Using standard procedure, the assay is used with samples having protein concentrations between 0.2 and 1.5 mg/ml and requires just 100 µl of sample. The procedure can easily be adapted to a low-concentration microassay (see below). The microplate assay reduces the volume of sample required to just 5 µl. The DC protein assay is measured at 650–750 nm with a standard laboratory spectrophotometer or microplate reader.

DC protein assay kits offer either bovine serum albumin or bovine γ-globulin standard sets.

Microassay Capability
For lower concentrations of protein, the microassay procedure can be used in a standard test tube or in a microplate format. The microassay procedure can be used to measure protein concentrations from 5 to 250 µg/ml; it requires 200 µl of sample for the test tube assay or 20 µl of sample for the microplate assay. When using the microassay format, the kit contains enough reagent for 2,500 standard assays or 25,000 microplate assays.

Because the ratio of sample to reagent is changed, compatibility changes when using the microassay. The DC protein microassay is not compatible with 2-mercaptoethanol, dithiothreitol, or guanidine HCl.

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5000111
Detergent-compatible colorimetric assay kit, includes 250 ml alkaline copper tartrate, 2 L dilute Folin reagent, 5 ml surfactant solution, bovine γ-globulin standard; 450 assays
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5000112
Detergent-compatible colorimetric assay kit, includes 250 ml alkaline copper tartrate, 2 L dilute Folin reagent, 5 ml surfactant solution, BSA standard; 450 assays

Accessories

Accessories

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(Discontinued) Protein Standard I
5000005
Pkg of 1 vial, lyophilized bovine γ-globulin; 20 ml (reconstituted)
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(Discontinued) Protein Standard II
5000007
Pkg of 1 vial, lyophilized bovine serum albumin, 20 ml (reconstituted)
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2239955
Pkg of 100, 1.5 ml cuvette
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2239950
Pkg of 100, 3.5 ml cuvette

Kits & Reagents

Kits & Reagents

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5000113
250 ml, alkaline copper tartrate solution for colorimetric assays; replacement reagent for DC protein assay kits and RC DC protein assay kits
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5000114
1 L, dilute Folin reagent for colorimetric assays; replacement reagent for DC protein assay kits and RC DC protein assay kits
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5000115
5 ml, surfactant solution for colorimetric assays; replacement reagent for DC protein assay kits and RC DC protein assay kits
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5000116
Reagent pack for colorimetric assays, includes 250 ml alkaline copper tartrate, 2 L dilute Folin reagent, 5 ml surfactant solution; 450 assays

Documents

Documents

Number Description Options
1123
Protein Assay Applications Bibliography
1069
Colorimetric Protein Assays, Rev D
1731
Bio-Rad DC Protein Assay Flier, Rev A.
LIT448
Instruction Manual, DC Protein Assay
1909
Modification of Bio-Rad DC Protein Assay for Use With Thiols
1770
Total Cellular Protein Determination Using the DC Protein Assay
6837
DC™ Protein Assay Quick Guide, Ver A
6852
Reagent Compatibility Chart for Bio-Rad Protein Assays Quick Guide,Ver A