Residual DNA Quantification Supermixes

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Overview

This digital PCR supermix enables highly precise and sensitive quantification of residual host cell DNA (HCD) without the need for DNA purification.

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Description

Description

 

Our ddPCR Supermix for Residual DNA Quantification is a 2x concentrated, ready-to-use digital pcr master mix optimized to deliver maximum PCR efficiency, specificity, and sensitivity for direct quantification of residual host cell DNA (HCD) with the Droplet Digital PCR (ddPCR) System.

Key Benefits

  • Highly precise and sensitive method for direct quantification of residual host cell DNA
  • No DNA purification required
  • Free of detectable E. coli, CHO, mouse, human, and yeast DNA
  • Contains all components required for hydrolysis probe–based ddPCR assays except primers, probe(s), and template
  • Limits nonspecific PCR amplification
  • Hot-start polymerase keeps enzyme inactive during sample droplet partitioning

Applications and Uses

  • Absolute quantification of residual HCD
  • Environmental monitoring
  • Food testing

Packaging Options

Number of 20 µl Reactions Supermix Volume, ml Catalog Number
200 2 x 1 1864037
500 5 x 1 1864038
2,500 5 x 5 1864039
5,000 10 x 5 1864040

 

Additional Reagents

Bio-Rad offers additional consumables for completing your digital PCR workflow:

Additional Equipment

This digital PCR supermix requires the use of one or more of the following instruments and accessories:

More Information

This ddPCR Supermix for Residual DNA Quantification is optimized for use with Droplet Generation Oil for Probes on the QX200 Droplet Digital PCR System and QX200 AutoDG Droplet Digital System.

Specifications

Specifications

Storage at –20°C
Up to 24 months (refer to expiration date)
Storage at 4°C (after thawing)
Up to 2 weeks
Template compatibility
cDNA, genomic DNA, plasmid DNA

Ordering

Ordering

items

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1864037
2 ml (2 x 1 ml), 2x supermix, for direct quantification of residual host cell DNA in the QX200™AutoDG™/ QX200™/QX100™ Droplet Digital™ PCR Systems
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1864038
5 ml (5 x 1 ml), 2x supermix, for direct quantification of residual host cell DNA in the QX200™AutoDG™/ QX200™/QX100™ Droplet Digital™ PCR Systems
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1864040
50 ml (10 x 5 ml), 2x supermix, for direct quantification of residual host cell DNA in the QX200™AutoDG™/ QX200™/QX100™ Droplet Digital™ PCR Systems
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ddpcr-supermix-residual-dna-quantification-5-tubes-186-4039-tn.jpg
1864039
25 ml (5 x 5 ml), 2x supermix, for direct quantification of residual host cell DNA in the QX200™AutoDG™/ QX200™/QX100™ Droplet Digital™ PCR Systems

Consumables

Consumables

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1814040
Pkg of 100, foil seals for PCR and QX200/QX100 Droplet Digital PCR System applications, for use with PX1 PCR Plate Sealer

Equipment

Equipment

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1864100

AutoDG Droplet Digital PCR System for EvaGreen or probe-based digital PCR applications, includes Automated Droplet Generator, QX200 Droplet Reader, computer, and QX Manager Software, Standard Edition.

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1864001

Droplet Digital PCR System, includes droplet generator, droplet reader, computer, software, associated component consumables, for EvaGreen or probe-based digital PCR applications

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1864101
AutoDG Instrument for automated droplet generation of up to 96 samples, for use with the QX200/QX100; Droplet Digital PCR Systems
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1864002

Droplet generator for EvaGreen or probe-based digital PCR applications, for use with QX200™ Droplet Digital™ PCR System

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1851197

Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, 96–deep well reaction module, USB flash drive

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(Discontinued) PX1 PCR Plate Sealer
1814000
PCR plate sealer, includes heat sealing instrument, plate support block that holds 96-well and 384-well plates, sealing frame, power cord

Documents

Documents

Number Description Options
6721
ddPCR™ Supermix for Residual DNA Quantification Flier, Rev A
10040718
Droplet Generation Oil for Probes Product Insert
6311
QX200 Droplet Digital PCR System Brochure, Rev C
[ Add to Cart (Free) ]
10043138
Instruction Manual, Automated Droplet Generator for Droplet Digital™ PCR Systems
10031907
Instruction Manual, QX200™ Droplet Generator, Rev C
10031906
Instruction Manual, QX200 Droplet Reader and QuantaSoft Software
10048259
ddPCR Supermix for Residual DNA Quantification Product Insert