Criterion™ XT Bis-Tris Precast Gels

Image
lsr_criterion_xt_bis_tris_precast_gels.jpg

Overview

Criterion XT precast gels are formulated at a near-neutral pH, significantly delaying acrylamide hydrolysis compared to traditional Laemmli systems. Their chemical composition allows maximum stability.
Read full description Ordering Information

Description

Description

Criterion™ XT gels are ideal for vertical midi protein gel electrophoresis, are designed to work with optimized sample and running buffers, and have an extended shelf life. Like traditional Laemmli systems, Criterion XT gels use discontinuous buffer ion fronts that form moving boundaries to stack and then separate proteins.

Features and Benefits

  • 12 month shelf life
  • Formulated using a Bis-Tris-HCl buffer system (pH 6.4) to separate proteins by molecular weight
  • Select from two running buffers to expand the separation capability of a single Bis-Tris gel type
  • Near-neutral pH significantly delays acrylamide hydrolysis as compared to traditional Laemmli systems
  • Run 2-D gels in less than a day

Applications and Uses

  • Polyacrylamide gel electrophoresis (PAGE or SDS-PAGE)
  • 2-D gel electrophoresis
  • Screen new samples
  • Evaluate sample preparation conditions

To further refine the resolution range, refer to the Protein Migration Chart for different acrylamide concentrations appropriate for your proteins of interest.

Specifications

Specifications

Gel dimensions (W x L x thickness)
13.3 x 8.7 x 0.1 cm
Cassette dimensions (W x L x thickness)
15.0 x 10.6 x 5.3 cm
Cassette material
Styrene copolymer
Comb material
Polycarbonate
Storage tray material
PET
Gel storage conditions
Store flat at room temperature; do not freeze
  Gel shelf life at recommended temperature*
12 months
Buffer volume
Upper, 60 ml; lower, 400 ml
Recommended sample buffer
XT sample buffer + XT reducing agent
Recommended running buffer
XT MES or XT MOPS
  Gel chemistry
Bis-Tris
* From date of manufacture.
 

Ordering

Ordering

items

Use the filters below to refine results!

Image
345-0123_tn.jpg
3450123
Pkg of 1, 4–12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
Image
345-0124_tn.jpg
3450124
Pkg of 1, 4–12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
Image
345-0125_tn.jpg
3450125
Pkg of 1, 4–12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
Image
345-0126_tn.jpg
3450126
Pkg of 1, 4–12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
Image
345-0111_tn.jpg
3450111
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
Image
345-0112_tn.jpg
3450112
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
Image
345-0113_tn.jpg
3450113
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
Image
345-0117_tn.jpg
3450117
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
Image
345-0118_tn.jpg
3450118
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
Image
345-0119_tn.jpg
3450119
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
Image
345-0120_tn.jpg
3450120

Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

Image
345-0127_tn.jpg
3450127
Pkg of 1, 4–12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
Image
345-0115_tn.jpg
3450115
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
Image
345-0121_tn.jpg
3450121
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

Accessories

Accessories

Kits & Reagents

Kits & Reagents

Image
161-0793_tn.jpg
1610793
Pkg of 1, electrophoresis buffer kit, includes 500 ml XT MOPS Running Buffer, 10 ml 4x XT Sample Buffer, 1 ml XT Reducing Agent
Image
161-0796_tn.jpg
(Discontinued) XT MES Buffer Kit
1610796
Pkg of 1, electrophoresis buffer kit, includes 500 ml 20x XT MES Running Buffer, 10 ml 4x XT Sample Buffer, 1 ml 20x XT Reducing Agent

Documents

Documents

Number Description Options
6040
Electrophoresis Guide, Interactive PDF, Rev C
2788
Criterion Blotting Tray Insert, Rev A
2912
Criterion XT Precast Gel Flier, Rev A
2710
Criterion™ Precast Gel System Brochure, Rev C
2911
Criterion XT Precast Gels Product Information Sheet, Rev B
4110001
Instruction Manual, Criterion™ Gel Application Guide, Ver H
4110130
Instruction Manual, Criterion XT Precast Gel Instruction Guide, Rev B