Criterion XT Bis-Tris Precast Gels
Criterion XT precast gels are formulated at a near-neutral pH, significantly delaying acrylamide hydrolysis compared to traditional Laemmli systems. Their chemical composition allows maximum stability.
Overview
Criterion XT gels are ideal for vertical midi protein gel electrophoresis, are designed to work with optimized sample and running buffers, and have an extended shelf life. Like traditional Laemmli systems, Criterion XT gels use discontinuous buffer ion fronts that form moving boundaries to stack and then separate proteins.
Features and Benefits
- 12 month shelf life
- Formulated using a Bis-Tris-HCl buffer system (pH 6.4) to separate proteins by molecular weight
- Select from two running buffers to expand the separation capability of a single Bis-Tris gel type
- Near-neutral pH significantly delays acrylamide hydrolysis as compared to traditional Laemmli systems
- Run 2-D gels in less than a day
Applications and Uses
- Polyacrylamide gel electrophoresis (PAGE or SDS-PAGE)
- 2-D gel electrophoresis
- Screen new samples
- Evaluate sample preparation conditions
To further refine the resolution range, refer to the Protein Migration Chart for different acrylamide concentrations appropriate for your proteins of interest.
Specifications
Gel dimensions (W x L x thickness)
13.3 x 8.7 x 0.1 cm
Cassette dimensions (W x L x thickness)
15.0 x 10.6 x 5.3 cm
Cassette material
Styrene copolymer
Comb material
Polycarbonate
Storage tray material
PET
Gel storage conditions
Store flat at room temperature; do not freeze**
Gel shelf life at recommended temperature*
12 months
Buffer volume
Upper, 60 ml; lower, 400 ml
Recommended sample buffer
XT sample buffer + XT reducing agent
Recommended running buffer
XT MES or XT MOPS
Gel chemistry
Bis-Tris
* From date of manufacture.
**Gels are shipped cold to reduce temperature fluctuations and can be temporarily stored at 2-8°C without affecting gel performance.
Ordering
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(Discontinued) 4–12% Criterion™ XT Bis-Tris Protein Gel, 12+2 well, 45 µl
3450123
Pkg of 1, 4–12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
Image
(Discontinued) 4–12% Criterion™ XT Bis-Tris Protein Gel, 18 well, 30 µl
3450124
Pkg of 1, 4–12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
Image
(Discontinued) 4–12% Criterion™ XT Bis-Tris Protein Gel, 26 well, 15 µl
3450125
Pkg of 1, 4–12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
Image
3450126
Pkg of 1, 4–12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
Image
(Discontinued) 10% Criterion™ XT Bis-Tris Protein Gel, 12+2 well, 45 µl
3450111
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
Image
(Discontinued) 10% Criterion™ XT Bis-Tris Protein Gel, 18 well, 30 µl
3450112
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
Image
(Discontinued) 10% Criterion™ XT Bis-Tris Protein Gel, 26 well, 15 µl
3450113
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
Image
(Discontinued) 12% Criterion™ XT Bis-Tris Protein Gel, 12+2 well, 45 µl
3450117
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
Image
(Discontinued) 12% Criterion™ XT Bis-Tris Protein Gel, 18 well, 30 µl
3450118
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
Image
(Discontinued) 12% Criterion™ XT Bis-Tris Protein Gel, 26 well, 15 µl
3450119
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
Image
3450127
Pkg of 1, 4–12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
Image
3450115
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
Image
3450121
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
Accessories
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(Discontinued) Mini-PROTEAN® Cassette Opening Lever
4560000
Pkg of 1, 100% aluminum and recyclable opening lever for Mini-PROTEAN precast gel cassettes
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(Discontinued) Gel Releasers
1653320
Pkg of 5, gel releaser for Mini-PROTEAN® Tetra system, Mini-PROTEAN® 3 Dodeca™ cell, Mini-PROTEAN 3 multi-casting chamber, and PROTEAN® Plus Dodeca™ cell
Kits & Reagents
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(Discontinued) XT MOPS Running Buffer
1610788
Pkg of 1, 500 ml, electrophoresis buffer for use with Criterion™ XT Bis-Tris Gels
Image
(Discontinued) XT MES Running Buffer
1610789
Pkg of 1, 500 ml, 20x electrophoresis buffer for use with Criterion™ XT Bis-Tris Gels
Image
(Discontinued) 20x XT MOPS Running Buffer Kit
1610793
Pkg of 1, electrophoresis buffer kit, includes 500 ml XT MOPS Running Buffer, 10 ml 4x XT Sample Buffer, 1 ml XT Reducing Agent
Image
(Discontinued) XT MES Buffer Kit
1610796
Pkg of 1, electrophoresis buffer kit, includes 500 ml 20x XT MES Running Buffer, 10 ml 4x XT Sample Buffer, 1 ml 20x XT Reducing Agent
Image
(Discontinued) XT Sample Buffer
1610791
10 ml, 4x premixed protein sample buffer for use with all Criterion XT gels
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(Discontinued) XT Reducing Agent
1610792
1 ml, 20x reducing agent for use with all Criterion XT gels
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