ZE5 Cell Analyzer

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Overview

The ZE5 Cell Analyzer is an innovative flow cytometer with flexible configurations to meet a broad range of experimental complexities and throughput needs – accessible for novice flow cytometry users yet flexible enough for the most experienced flow cytometry professionals.

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Description

Description

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The ZE5 Cell Analyzer is the only flow cytometer with the ideal combination of production throughput, smart sample-handling and superior speed and sensitivity to meet your research needs.

High-throughput Production

  • Detect up to 100,000 events per second without compromising data quality
  • Low sample carryover with built-in auto probe wash
  • Integrated 96/384/deep-well plate sample loader

Smart Sample-handling

  • Temperature control and vortexing to maintain cell viability and prevent clogging
  • Innovative sample pump that takes up samples with minimal dead volume and returns unused sample

Multi-parameter Analysis

  • Up to five lasers and 30 detectors including seven colors off UV for building larger panels, or panels with minimal compensation
  • Small particle detector in 3-5 laser configuration for studying exosomes and other small particles

Automation/Robotics-friendly

  • API to connect to LIMS and scheduling software for integration to existing or new workcells

Large Capacity Fluidics Options

The external house deionized (DI) water connection is available for laboratories with access to pressurized house DI water. The external carboy DI water and waste connections include a fluidics cart and an 18.9 L carboy that allows around 22 hours of continuous run time. The ZE5 Cell Analyzer is enabled for hot swap and alerts of remaining run time of fluidics levels from the Everest software. The waste carboy includes protection from overflow.

Small Particle Detector Upgrade Kit (405 nm laser)

This upgrade kit enables small particle detection. A secondary forward scatter detector is available for three- and four-laser ZE5 Cell Analyzers with a 405 nm laser. Note: The small particle detector is included with the five-laser ZE5 Cell Analyzer.

Laser Upgrade Kits

The ZE5 Cell Analyzer laser upgrade kits expand experimental options with an increase from five to seven detectors for the 355 nm laser. They can be used in research applications using ultraviolet (UV) excitable dyes. Kits are available in two filter configurations. The UV laser addition for any ZE5 Cell Analyzer without a UV laser adds the 355 nm laser and bank with five detectors.

Everest Software Upgrade for Automation of ZE5 Cell Analyzer

The Everest application program interface and software development kit allow the ZE5 Cell Analyzer to interface with existing automation systems.

FCS Express Flow Cytometry Analysis Software

FCS Express Software is a fully integrated analysis, statistics, graphing and reporting tool for getting flow cytometry results. FCS Express utilizes a familiar interface for quick learning and integrated spreadsheets so your graphs and statistics update as your gates change. When you are ready to publish and present your data, FCS Express has you covered by supporting direct high-resolution exports to PowerPoint, PDF, and six image formats at up to 700 DPI resolution. Importantly, FCS Express is optimized to support and display data acquired from all Bio-Rad flow cytometry instruments. FCS Express may be configured with additional options available directly from DeNovo Software to help meet 21 CFR part 11 compliance. Please contact De Novo at support@denovosoftware.com for more information.

Specifications

Specifications

Fluorescence sensitivity
Average MESF: FITC: <70 PE: <50 and APC: <40
Scatter sensitivity
<0.2 μm FSC resolution with small-particle detection module
Sample loading
96-well and 384-well microplates, standard or deep-well
40-tube rack of 5 ml tubes (12 x 75 mm)
24-tube rack of microcentrifuge tubes
STAT tube position for single tube loading
Sample throughput
<15 min for 96-well plate and < 60 min for 384-well plate in high-throughput mode
Excitation sources 
Up to five spatially separated lasers. Standard options include:
355 nm, 50 mW
488 nm, 100 mW
640 nm, 100 mW
405 nm, 100 mW
561 nm, 50 mW
Detection
Up to 30 detectors, including FSC and SSC; optional second FSC detector
Cuvette
Fused silica with 145 x 265 μm channel
Speed
Up to 100,000 events per second with all parameters enabled
Data processing
Simultaneous measured peak, area, and width for every channel
24-bit data for area and height
17-bit data for width with high-resolution linear interpolation at the half height
Fluidics
Bulk fluids: 4 x 4 L bulk fluid tanks on board for sheath and waste
Onboard additive concentrate and cleaner
Operational flow rates
0.025–3.5 μl/sec
Dimensions (W x D x H)
74 x 69 x 66 cm (29 x 27 x 26 in)
Weight
110 kg (240 lb) maximum
Data format
Flow Cytometry Standard (FCS) 3.1

Ordering

Ordering

items

Use the filters below to refine results!

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12004279

ZE5 Cell Analyzer; flow cytometer with 355 nm, 405 nm, 488 nm, 561 nm, and 640 nm lasers, 27 fluorescence detectors, plus SSC, dual FSC, Everest software, flexible sample loader, ZE5 EYE, integrated fluidics

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12014135

ZE5 Cell Analyzer; flow cytometer with 355 nm, 405 nm, 488 nm, 561 nm, 640 nm lasers, and 27 fluorescence detectors (7 detectors off UV Option A), plus FSC, SSC and a small particle detector (SPD)

ze5-cell-analyzer-tn.jpg
12014136

ZE5 Cell Analyzer; flow cytometer with 355 nm, 405 nm, 488 nm, 561 nm, 640 nm lasers, and 27 fluorescence detectors (7 detectors off UV Option B), plus FSC, SSC and a small particle detector (SPD)

ze5-cell-analyzer-tn.jpg
12004278

ZE5 Cell Analyzer; flow cytometer with 405 nm, 488 nm, 561 nm, and 640 nm lasers, 24 fluorescence detectors, plus SSC, FSC, Everest software, flexible sample loader, ZE5 EYE, integrated fluidics

ze5-cell-analyzer-tn.jpg
12014138

ZE5 Cell Analyzer; flow cytometer with 405 nm, 488 nm, 561 nm, 640 nm lasers, and 24 fluorescence detectors, FSC, SSC plus a small particle detector (SPD)

ze5-cell-analyzer-tn.jpg
12004277

ZE5 Cell Analyzer; flow cytometer with 488 nm, 561 nm, and 640 nm lasers, 17 fluorescence detectors, plus SSC, FSC; Everest software, flexible sample loader, ZE5 EYE, integrated fluidics

ze5-cell-analyzer-tn.jpg
12014139

ZE5 Cell Analyzer; flow cytometer with 405 nm, 488 nm, 561 nm lasers, and 20 fluorescence detectors, plus FCS and SSC

ze5-cell-analyzer-tn.jpg
12004276

ZE5 Cell Analyzer, flow cytometer with 405 nm, 488 nm, and 640 nm lasers, 17 fluorescence detectors, plus SSC, FSC, Everest software, flexible sample loader, ZE5 EYE, integrated fluidics

ze5-cell-analyzer-tn.jpg
12014141

ZE5 Cell Analyzer; flow cytometer with 405 nm, 488 nm, 640 nm lasers, and 17 fluorescence detectors, plus FCS, SSC, and a small particle detector (SPD)

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12009707
External fluidics connections reduce day-to-day fluidics maintenance and enable uninterrupted overnight runs in high-throughput and automation environments
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12009734
External fluidics connections reduce day-to-day fluidics maintenance and enable uninterrupted overnight runs in high-throughput and automation environments
12009651
External fluidics connections reduce day to day fluidics maintenance and enable uninterrupted overnight runs in high-throughput and automation environments. Suitable for laboratories with access to pressurized house DI water
12009761
The Everest API & SDK (application program interface, software developer kit) allows the ZE5 Cell Analyzer to interface with existing automation systems.
12009028
This upgrade kit expands experimental options by increasing the number of detectors off the 355 nm laser from 5 to 7. Compatible systems: ZE5 Systems with 5 lasers.
12009029
This upgrade kit expands experimental options by increasing the number of detectors of the 355 nm laser from 5 to 7. Compatible systems: ZE5 System with 5 lasers.
12009185
The UV (355 nm) laser addition is suitable for any ZE5 System without UV laser. It adds the 355 nm laser and bank with 5 detectors. Compatible systems: any ZE5 System without a UV laser.
12009372
This upgrade kit makes an SPD (a secondary FSC detector off the 405 nm laser) available for 3 and 4 laser ZE5 Systems with a 405 nm laser. Note: The SPD comes standard on 5 laser ZE5 Cell Analyzer.

Accessories

Accessories

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12013092
A comprehensive analysis software developed by De Novo Software that combines data analysis, statistics, graphing and reporting functionalities for flow and image cytometry
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12013095
A comprehensive analysis software developed by De Novo Software that combines data analysis, statistics, graphing and reporting functionalities for flow and image cytometry
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12010522
ZE5 External Waste Carboy Cap with Quick Disconnect, cap with overflow sensor, leak-free Quick Disconnect, and filtered vent
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12010523
ZE5 DI Water Carboy Cap, vented with integrated replaceable filter and leak-free Quick Disconnect
12010480
ZE5 Waste Carboy, 20 L, collect waste from a ZE5 Cell Analyzer that is connected to ZE5 External Waste Fluidics Cart
12010521
ZE5 DI Water Carboy, 20 L, supply deionized water to a ZE5 Cell Analyzer that is connected to the ZE5 External DI Water Fluidics Cart
12004404
Pkg of 1, additive bottle with white cap, tubing, filter and quick connector, for use on any ZE5 Cell Analyzer
12004395
Pkg of 1, cytometer cleaner bottle, includes blue cap, tubing, filter, and quick connector, for use on any ZE5 Cell Analyzer
12004444
Pkg of 2, cleaner/additive bottles, 450 ml, for use with ZE5 Additive or Cytometer Cleaner on any ZE5 Cell Analyzer
12004397
Pkg of 1, DI water container, 4 L, includes cap, tubing, filters, and quick connectors, for use on any ZE5 Cell Analyzer
12004396
Pkg of 1, waste container, 4 L, includes cap/tubing assembly with quick connectors, for use on any ZE5 Cell Analyzer
12004402
Pkg of 2, waste containers, 4 L, for use on any ZE5 Cell Analyzer; cap/tubing assembly not included
12004418
Pkg of 2, DI water containers, 4 L, for use on any ZE5 Cell Analyzer; cap/tubing assembly not included
12004274
Pkg of 25, 5 ml test tubes, 12 x 75 mm, sterile polypropylene, with caps
12004445
Pkg of 1, tube rack for 5 ml (12 x 75 mm) test tubes, holds 40 tubes, for use on any ZE5 Cell Analyzer
12004446
Pkg of 1, tube rack for 1.5 ml microfuge tubes, holds 24 tubes, for use on any ZE5 Cell Analyzer
(Discontinued) Bi-Level Tube Lifter
12005002
Pkg of 1, bi-level tube lifter, for use with the ZE5™ 5 ml Tube Rack
12005773
Pkg of 10, clear polystyrene U-bottom 96-well microplates, validated for use with the ZE5™ Cell Analyzer

Consumables

Consumables

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12012577
Pkg of 100, non-sterile, cup-fitting, and disposable cell filters, 30 µm. Used to remove aggregates and to prepare uniform single-cell suspensions from tissue, fluids, plant material, nuclei, etc.
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12012582
Pkg of 100, non-sterile, cup fitting, and disposable cell filters, 50 µm. Used to remove aggregates and to prepare uniform single-cell suspensions from tissue, fluids, plant material, nuclei, etc.
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12012575
Pkg of 100, non-sterile, cup fitting, and disposable cell filters, 70 µm, Used to remove aggregates and to prepare uniform single-cell suspensions from tissue, fluids, plant material, nuclei, etc.
12004403
Includes 3–3.4 μm single-peak beads containing dyes that emit in all fluorescence channels from ultraviolet to far red. Designed for checking optical alignment in all channels of the ZE5™ Cell Analyzer.
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1451085
5 ml (1 x 5 ml), include 3.0–3.4 µm beads dyed with 8 different fluorescent intensities for testing sensitivity and linearity on any S3™ or S3e™ Cell Sorter
(Discontinued) ZE5™ Additive
12004271
1.2 L (4 x 300 ml), ZE5 Additive, for use with the ZE5™ Cell Analyzer to manage bubbles and prevent microbial growth
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1451083

20 L (5 x 4 L), ready-to-use, sterile endotoxin-free water in gamma-irradiated S3e Fluidic Containers, for use on any S3 or S3e Cell Sorter

(Discontinued) Cytometer Cleaner
12004272
1 L, cytometer cleaner, for use with the ZE5™ Cell Analyzer

Reagents

Reagents

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1351303
Pkg of 5 vials, 50 µg each, DAPI nuclear staining dye powder
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1351304
Pkg of 5 vials, 56 µg each, Hoechst 33342 nuclear staining dye powder
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(Discontinued) ReadiDrop™ 7-AAD
1351102
9 ml, (3 x 3 ml) ready-to-use dropper bottles for dead cell exclusion
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1351101
9 ml, (3 x 3 ml) ready-to-use dropper bottles for dead cell exclusion
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1351111
Pkg of 1, kit includes 4 x 50 assay vials and 250 µl of DMSO, for running 200 assays
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1351112
Pkg of 1, kit includes 4 x 50 assay vials and 250 µl of DMSO, for running 200 assays
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1351113
Pkg of 1, kit includes 4 x 50 assay vials and 250 µl of DMSO, for running 200 assays
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1351114
Pkg of 1, kit includes 4 x 50 assay vials and 250 µl of DMSO, for running 200 assays
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1351115
Pkg of 1, kit includes 4 x 50 assay vials and 250 µl of DMSO, for running 200 assays
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1351116
Pkg of 1, kit includes 4 x 50 assay vials and 250 µl of DMSO, for running 200 assays
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1351117
Pkg of 1, kit includes 4 x 50 assay vials and 250 µl of DMSO, for running 200 assays
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1351118
Pkg of 1, kit includes 4 x 50 assay vials and 250 µl of DMSO, for running 200 assays
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1351202

Pkg of 1, kit includes 4 x 50 assay vials and 250 μl of DMSO, for running 200 assays

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1351203

Pkg of 1, kit includes 4 x 50 assay vials and 250 μl of DMSO, for running 200 assays

cell-proliferation-dyes-135-1204-thumb.jpg
1351204

Pkg of 1, kit includes 4 x 50 assay vials and 250 μl of DMSO, for running 200 assays

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1351205

Pkg of 1, kit includes 4 x 50 assay vials and 250 µl of DMSO, for running 200 assays

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(Discontinued) CFDA-SE
1351201
Pkg of 1, 5 x 100 µg vials
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1351011
Pkg of 1, kit includes dye, reaction buffer, and quench buffer for labeling 2 x 50 µg of antibodies
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1351002
Pkg of 1, kit includes dye, reaction buffer, and quench buffer for labeling 2 x 50 µg of antibodies
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1351003
Pkg of 1, kit includes dye, reaction buffer, and quench buffer for labeling 2 x 50 µg of antibodies
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1351004
Pkg of 1, kit includes dye, reaction buffer, and quench buffer for labeling 2 x 50 µg of antibodies
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1351005
Pkg of 1, kit includes dye, reaction buffer, and quench buffer for labeling 2 x 50 µg of antibodies
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1351006
Pkg of 1, kit includes dye, reaction buffer, and quench buffer for labeling 2 x 50 µg of antibodies
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1351007
Pkg of 1, kit includes dye, reaction buffer, and quench buffer for labeling 2 x 50 µg of antibodies
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1351008
Pkg of 1, kit includes dye, reaction buffer, and quench buffer for labeling 2 x 50 µg of antibodies

Download

Download

ZE5 Everest Software v.3.1
SOFT-ZE5EVEREST_V3.1

 ZE5 Everest Software v.3.1

Documents

Documents

Number Description Options
7250
From Discovery to Manufacturing: A Workflow for Protein-Based Immunotherapies
7424
From Immune Surveillance to Therapeutic Development
7295
Automation of High-Throughput Flow Cytometry with the ZE5 Cell Analyzer
7125
Utilizing the multiparameter capability of the ZE5 to Monitor T Cell Exhaustion and Effects of Immunotherapy
7101
Flow Cytometry–Based Exosome Detection on the ZE5
7102
Small Particle Detection and Analysis on the ZE5 Cell Analyzer Poster
7104
Elucidating Complex Flow Cytometry Studies with the Speed and Sensitivity of the ZE5 Cell Analyzer Poster
7105
Utilizing the Multiparameter Capability of the ZE5 Cell Analyzer to Monitor T-Cell Exhaustion Following Immunotherapy Poster
6814
ZE5 Cell Analyzer Brochure
[ Add to Cart (Free) ]
7069
Get more from your experiments with the ZE5™ Cell Analyzer
[ Add to Cart (Free) ]
7301
FCS Express Software for ZE5 Cell Analyzer Users Flier
7288
ZE5 Cell Analyzer Data Scaling Settings in Third-Party Analysis Software Quick Guide
10000072648
ZE5™ Cell Analyzer Quick Guide
10000072647
ZE5™ Cell Analyzer and Everest™ Sofware User Guide
10000086713
ZE5 Everest Software v.3.1 Release Notes
10000071332
ZE5™ Cell Analyzer Additive Product Information Sheet
10000071333
ZE5™ Cell Analyzer QC Beads Product Information Sheet
10000071640
Cytometer Cleaner Product Information Sheet
6950
Optimized Twenty-One Color Panel Design Using the ZE5™ Cell Analyzer for Quantification of T Cell Subsets in Stem Cell Transplant Patients
6962
Analysis of Mixed Microbial Populations and Deep Immunotyping of Peripheral Blood on the ZE5™ Cell Analyzer
6993
A 19-Color Multiparameter White Blood Cell Panel Designed for the Immunophenotyping of Normal and Malignant Leukocytes by Flow Cytometry
10032248
ProFlow™ Sort Grade Water Product Insert
10033040
ProLine™ Rainbow Beads Product Insert
6999
Flow Experiments Come Alive
10000076988
ZE5 Cell Analyzer Reagent Reference Guide
6523
ReadiLink Antibody Labeling Kits Flier, Rev C
[ Add to Cart (Free) ]
10033055
ReadiLink Antibody Labeling Kit Product Insert, Rev C
6522
ReadiDrop Cell Viability Assays Flier, Rev B
[ Add to Cart (Free) ]
10049240
ReadiDrop™ Cell Viability Assays Product Insert, Rev A
10041645
VivaFix™ Cell Viability Assay Product Insert, Rev B
6559
VivaFix™ Cell Viability Assays Flier, Rev B
[ Add to Cart (Free) ]
10043282
PureBlu™ DAPI Nuclear Staining Dye Product Insert, Rev A
10043283
PureBlu™ Hoechst 33342 Nuclear Staining Dye Product Insert, Rev A
6607
PureBlu™ Nuclear Staining Dyes Flier, Ver B
[ Add to Cart (Free) ]
7122
Moving Forward with Immunotherapies for Cancer
10000109992
ZE5 External House DI Upgrade Kit, Rev A
1000111720
ZE5 External DI Water Carboy Upgrade Kit User Guide, Rev A
7215
ZE5 Cell Analyzer Publications List
10000121934
Product Information sheet, ProFlow Cell Filters for Flow Cytometry
10000128253
Maintaining the ZE5 Cell Analyzer When Not in Use