PureBlu™ DAPI Nuclear Staining Dye #1351303

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Overview

Pkg of 5 vials, 50 µg each, DAPI nuclear staining dye powder
Safety Data Sheet

Safety Data Sheet

Description

Description

Use PureBlu DAPI Dye for routine nuclear staining in fluorescence microscopy and cell imaging applications. PureBlu DAPI Dye is a highly pure formulation of DAPI in a user-friendly format allowing easy preparation of the working solution, with no weighing step and only a single dilution after resuspension.

DAPI (4',6-diamidino-2-phenylindole) is a well-characterized blue-emitting fluorescent compound widely utilized for nuclear staining. DAPI permeates cell membranes and binds to the minor groove of A/T-rich dsDNA sequences, thus preferentially staining nuclei (Figure 1).

DAPI binding dsDNA

Fig. 1. DAPI binding dsDNA.
 

When bound to dsDNA, PureBlu DAPI Dye has a maximum excitation wavelength in the ultraviolet range (359 nm), and the dye can be optimally detected in the blue channel with an emission maximum of 461 nm (Figure 2). The characteristic Stokes shift between excitation and emission wavelengths is fairly wide for DAPI, making this dye an optimal choice when good spectral separation is desired to reduce fluorescence interference, for example, in chromatin counterstaining for immunofluorescence microscopy.


DAPI emission spectrum

Fig. 2. DAPI excitation and emission spectra.
 

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More Information

PureBlu™ DAPI Nuclear Staining Dye Product Insert

Specifications

Specifications

Molecular formula
C16H15N5·2HCl
Molecular weight
350.25
Excitation/emission wavelengths
359/461 nm
CAS Number
28718-90-3
Purity
>95% (HPLC purified)
Solubility
Soluble in water and dimethyl sulfoxide (DMSO)
Storage and use
Long-term storage at –20ºC; protect from light
Temperature stability
Stable for 2 years at –20°C
After resuspension: stable for 1 year at –20°C or 6 months at 2–8°C