Overview
The ZE5 Cell Analyzer is an innovative, versatile flow cytometer designed to deliver powerful advancements in the quality and quantity of data that you can generate. It is versatile enough to handle complex assays and able to deliver data at a rate unmatched by other instruments.
Versatile
The ZE5 Cell Analyzer has a host of attributes that make it perfect for a wide range of applications and workflows:
- Allows complex immunophenotyping with up to 5 lasers and 30 detection parameters
- Adapts to your workflow, with a universal sample loader that can accommodate a wide range of sample formats and has integrated vortexing and temperature control
- Powers through high throughput and screening assays with fast plate handling, allowing 96-well plates to be completed in less than 15 minutes and 384-well plates in less than 60 minutes
- Detects rare events faster with an acquisition rate of up to 100,000 events/second without losing data due to electronic aborts, ideal for stem cell analysis
- Resolves small particles, including exosomes, with a dedicated small particle detector
- Performs advanced analysis with FCS Express analysis software
Easy to Use
Designed for busy multi-user laboratories and with an emphasis on ease of use, it incorporates innovative features that make it ideal for users with any level of experience:
- Learn, use, and train with ease with intuitive software and a unique experiment builder wizard
- Fluidics that never run dry with dry sample detection and live sheath and waste tank monitoring
- Self-cleaning with on-board cleaner
- QC at the touch of a button with on-board QC beads
Automation and 21 CFR Part 11 Ready
If you are considering flow cytometry for high throughput screening or pre-clinical applications that require 21 CFR Part 11 compliance, the ZE5 Cell Analyzer is an ideal choice.
- Designed to allow easy integration into robotic workcells
- Hands-off operation with optional large capacity fluidics
- Widely compatible with new or existing robotic workcells with a hardware and software agnostic application programming interface (API)
- Data acquisition with 21 CFR Part 11 support using Xybion Compliance Builder software
- Advanced data analysis with 21 CFR Part 11 support with FCS express analysis software (contact support@denovosoftware.com for more information)
- IQ/OQ service available
With so much to offer, the ZE5 Cell Analyzer may be the only flow cytometer you will ever need. To help you assess your needs and choose the correct configuration for you, please see our ZE5 Cell Analyzer Buyer's Guide.
For resources and support using your ZE5 Cell Analyzer visit our ZE5 Cell Analyzer resources page
Video Highlights
-
Image
Acquisition-to-Results Experience
-
Image
Meet the ZE5 Cell Analyzer from Bio-Rad
-
Image
Meet the ZE5 Designers: Dan Fox
-
Image
A Core Lab Manager's Perspective
Antibodies and Reagents
Suitable antibodies and reagents are critical components for HTS. Bio-Rad is dedicated to providing cutting edge reagents for all flow cytometry applications, and has recently launched an exclusive range of StarBright Dyes to allow you to build superior panels. Bio-Rad also has an extensive range of high-quality antibodies and reagents validated for flow cytometry, to help you conduct successful experiments.
Configurations
Upgrades
Software
Specifications
40-tube rack of 5 ml tubes (12 x 75 mm)
24-tube rack of microcentrifuge tubes
STAT tube position for single tube loading
355 nm, 50 mW
488 nm, 100 mW
640 nm, 100 mW
405 nm, 100 mW
561 nm, 50 mW
24-bit data for area and height
17-bit data for width with high-resolution linear interpolation at the half height
Onboard additive concentrate and cleaner
Ordering
items
Use the filters below to refine results!
ZE5 Cell Analyzer; flow cytometer with 355 nm, 405 nm, 488 nm, 561 nm, and 640 nm lasers, 27 fluorescence detectors, plus SSC, dual FSC, Everest software, flexible sample loader, ZE5 EYE, integrated fluidics
ZE5 Cell Analyzer; flow cytometer with 355 nm, 405 nm, 488 nm, 561 nm, 640 nm lasers, and 27 fluorescence detectors (7 detectors off UV Option A), plus FSC, SSC and a small particle detector (SPD)
ZE5 Cell Analyzer; flow cytometer with 355 nm, 405 nm, 488 nm, 561 nm, 640 nm lasers, and 27 fluorescence detectors (7 detectors off UV Option B), plus FSC, SSC and a small particle detector (SPD)
ZE5 Cell Analyzer; flow cytometer with 405 nm, 488 nm, 561 nm, and 640 nm lasers, 24 fluorescence detectors, plus SSC, FSC, Everest software, flexible sample loader, ZE5 EYE, integrated fluidics
ZE5 Cell Analyzer; flow cytometer with 405 nm, 488 nm, 561 nm, 640 nm lasers, and 24 fluorescence detectors, FSC, SSC plus a small particle detector (SPD)
ZE5 Cell Analyzer; flow cytometer with 488 nm, 561 nm, and 640 nm lasers, 17 fluorescence detectors, plus SSC, FSC; Everest software, flexible sample loader, ZE5 EYE, integrated fluidics
ZE5 Cell Analyzer; flow cytometer with 405 nm, 488 nm, 561 nm lasers, and 20 fluorescence detectors, plus FCS and SSC
ZE5 Cell Analyzer; flow cytometer with 405 nm, 488 nm, 561 nm lasers, and 20 fluorescence detectors: FSC, SSC plus a small particle detector (SPD)
ZE5 Cell Analyzer, flow cytometer with 405 nm, 488 nm, and 640 nm lasers, 17 fluorescence detectors, plus SSC, FSC, Everest software, flexible sample loader, ZE5 EYE, integrated fluidics
ZE5 Cell Analyzer; flow cytometer with 405 nm, 488 nm, 640 nm lasers, and 17 fluorescence detectors, plus FCS, SSC, and a small particle detector (SPD)
Accessories
A comprehensive analysis software developed by De Novo Software that combines data analysis, statistics, graphing and reporting functionalities for flow and image cytometry
A comprehensive analysis software developed by De Novo Software that combines data analysis, statistics, graphing and reporting functionalities for flow and image cytometry
ZE5 External Waste Carboy Cap with Quick Disconnect, cap with overflow sensor, leak-free Quick Disconnect, and filtered vent
ZE5 DI Water Carboy Cap, vented with integrated replaceable filter and leak-free Quick Disconnect
ZE5 Waste Carboy, 20 L, collect waste from a ZE5 Cell Analyzer that is connected to ZE5 External Waste Fluidics Cart
ZE5 DI Water Carboy, 20 L, supply deionized water to a ZE5 Cell Analyzer that is connected to the ZE5 External DI Water Fluidics Cart
Pkg of 1, additive bottle with white cap, tubing, filter and quick connector, for use on any ZE5 Cell Analyzer
Pkg of 1, cytometer cleaner bottle, includes blue cap, tubing, filter, and quick connector, for use on any ZE5 Cell Analyzer
Pkg of 2, cleaner/additive bottles, 450 ml, for use with ZE5 Additive or Cytometer Cleaner on any ZE5 Cell Analyzer
Pkg of 1, DI water container, 4 L, includes cap, tubing, filters, and quick connectors, for use on any ZE5 Cell Analyzer
Pkg of 1, waste container, 4 L, includes cap/tubing assembly with quick connectors, for use on any ZE5 Cell Analyzer
Pkg of 2, waste containers, 4 L, for use on any ZE5 Cell Analyzer; cap/tubing assembly not included
Pkg of 2, DI water containers, 4 L, for use on any ZE5 Cell Analyzer; cap/tubing assembly not included
Pkg of 1, tube rack for 5 ml (12 x 75 mm) test tubes, holds 40 tubes, for use on any ZE5 Cell Analyzer
Pkg of 1, tube rack for 1.5 ml microfuge tubes, holds 24 tubes, for use on any ZE5 Cell Analyzer
Pkg of 1, bi-level tube lifter, for use with the ZE5™ 5 ml Tube Rack
External fluidics connections reduce day-to-day fluidics maintenance and enable uninterrupted overnight runs in high-throughput and automation environments
This upgrade kit makes an SPD (a secondary FSC detector off the 405 nm laser) available for 3 and 4 laser ZE5 Systems with a 405 nm laser. Note: The SPD comes standard on 5 laser ZE5 Cell Analyzer.
This upgrade kit makes an SPD (a secondary FSC detector off the 405 nm laser) available for 3 and 4 laser ZE5 Systems with a 405 nm laser. Note: The SPD comes standard on 5 laser ZE5 Cell Analyzer.
External fluidics connections reduce day-to-day fluidics maintenance and enable uninterrupted overnight runs in high-throughput and automation environments
External fluidics connections reduce day to day fluidics maintenance and enable uninterrupted overnight runs in high-throughput and automation environments. Suitable for laboratories with access to pressurized house DI water
The Everest API & SDK (application program interface, software developer kit) allows the ZE5 Cell Analyzer to interface with existing automation systems.
This upgrade kit expands experimental options by increasing the number of detectors off the 355 nm laser from 5 to 7. Compatible systems: ZE5 Systems with 5 lasers.
This upgrade kit expands experimental options by increasing the number of detectors of the 355 nm laser from 5 to 7. Compatible systems: ZE5 System with 5 lasers.
The UV (355 nm) laser addition is suitable for any ZE5 System without UV laser. It adds the 355 nm laser and bank with 5 detectors. Compatible systems: any ZE5 System without a UV laser.
Consumables
Includes 3–3.4 μm single-peak beads containing dyes that emit in all fluorescence channels from ultraviolet to far red. Designed for checking optical alignment in all channels of the ZE5™ Cell Analyzer.
1.2 L (4 x 300 ml), ZE5 Additive, for use with the ZE5™ Cell Analyzer to manage bubbles and prevent microbial growth
1 L, cytometer cleaner, for use with the ZE5™ Cell Analyzer
5 ml (1 x 5 ml), include 3.0–3.4 µm beads dyed with 8 different fluorescent intensities for testing sensitivity and linearity on any S3™ or S3e™ Cell Sorter
20 L (5 x 4 L), ready-to-use, sterile endotoxin-free water in gamma-irradiated S3e Fluidic Containers, for use on any S3 or S3e Cell Sorter
Pkg of 25, 5 ml test tubes, 12 x 75 mm, sterile polypropylene, with caps
Pkg of 10, clear polystyrene U-bottom 96-well microplates, validated for use with the ZE5™ Cell Analyzer
Reagents
View All
Pkg of 5 vials, 50 µg each, DAPI nuclear staining dye powder
Pkg of 5 vials, 56 µg each, Hoechst 33342 nuclear staining dye powder
Downloads
ZE5 Everest Software v.3.2