TGX Stain-Free™ FastCast™ Acrylamide Solutions

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Overview

TGX Stain-Free FastCast acrylamide kits for hand casting polyacrylamide gels enable fast casting, long shelf life gels, short run times, high transfer efficiency, and protein visualization in 5 minutes with stain-free technology.
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Description

Description

What Is Stain-Free Technology?

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TGX Stain-Free FastCast premixed acrylamide solutions bring all the benefits of TGX gel chemistry to handcast polyacrylamide gels. TGX (Tris-Glycine eXtended) formulation retains Laemmli-like separation characteristics while using a standard Tris-glycine running buffer system. TGX Stain-Free FastCast premixed acrylamide solutions for hand casting are available in the most commonly used cross-linking ratios for PAGE systems.

TGX Stain-Free chemistry enables rapid fluorescent detection of proteins directly within gels and blots with Bio-Rad's stain-free enabled imagers. Protein samples can be separated in 20 minutes and then visualized in 5 minutes.

Features and Benefits of TGX Stain-Free FastCast Acrylamide Solutions

  • Handcast gels ready to run in as little as 30 min
  • Long shelf life — handcast gels remain fresh for up to 4 weeks at 4°C
  • Stain-free visualization of proteins in your gel or blot in less than 5 min with stain-free enabled imaging systems (Gel Doc™ EZ, Gel Doc XR+, ChemiDoc™ MP, ChemiDoc XRS+, and ChemiDoc Touch Systems)
  • Electrophoresis run times as short as 20 min using the Mini-PROTEAN® Tetra or Criterion™ cells
  • Efficient protein transfers in as little as 3 min using the Trans-Blot® Turbo™ transfer system
  • Ability to monitor the success of your electrophoresis and transfer steps without additional staining

Applications and Uses of TGX Stain-Free FastCast Acrylamide Solutions

  • 1-D polyacrylamide gel electrophoresis for separation of proteins
  • Accurate molecular weight estimation
  • Robust separation for complex samples

Approximate number of gels per kit.

  Mini Gels (~9 x 7 cm) Midi Gels (~13 x 9 cm)
  Mini-PROTEAN
Cassettes
0.75 mm
Glass Plates
1.0 mm
Glass Plates
1.5 mm
Glass Plates
Criteron
Cassettes
Starter Kit 11 gels 19 gels 13 gels 9 gels 7 gels
Full Kit 55 gels 95 gels 65 gels 45 gels 35 gels

More Information

TGX FastCast tutorial video Watch the TGX FastCast tutorial video to see how quick and easy it is to cast gels with TGX FastCast acrylamide kits. View step-by-step instructions for casting mini gels using the Mini-PROTEAN Tetra casting module.

TGX and TGX Stain-Free FastCast Acrylamide Kits: Tips & Tricks guideLearn how to cast better gels with Bio-Rad’s TGX and TGX Stain-Free FastCast Acrylamide Kits: Tips & Tricks guide.


 

FastCast kits calculator Download the FastCast kits calculator to automatically calculate the volumes of stacker, resolver, APS, and TEMED required when casting TGX FastCast and TGX Stain-Free™ FastCast gels.

Stain-Free Imaging

Bio-Rad's V3 Western Workflow™ is a five-step approach to streamlining your western blotting protocol. The V3 Western Workflow facilitates speed and validation at each step of a western blotting experiment — from running gels to quantifying proteins. View the V3 Western Workflow video to learn more.

The Quick Start Guide to Stain-Free Imaging provides step-by-step instructions on how to obtain stain-free gel images from TGX Stain-Free gels in less than 5 minutes using Bio-Rad’s ImageLab™ software and stain-free enabled imagers.

Bio-Rad Gel Electrophoresis Systems

Gel handcasting equipment and electrophoresis cells are offered in mini and midi gel formats. See the electrophoresis system selection guide for more details.

Midi gel systems:

Download the FastCast kits calculator to automatically calculate the volumes of stacker, resolver, APS, and TEMED required when casting TGX FastCast and TGX Stain-Free™ FastCast gels.

Learn how to cast better gels with Bio-Rad’s TGX and TGX Stain-Free FastCast Acrylamide Kits: Tips & Tricks guide.

Watch the TGX FastCast tutorial video to see how quick and easy it is to cast gels with TGX FastCast acrylamide kits. View step-by-step instructions for casting mini gels using the Mini-PROTEAN Tetra casting module.

Bio-Rad Gel Electrophoresis Systems

Gel handcasting equipment and electrophoresis cells are offered in mini and midi gel formats. See the electrophoresis system selection guide for more details.

Midi gel systems:

Criterion and Criterion™ Dodeca™ cells use integral cassettes for gel hand casting. 1.0 mm thick gel casting sets are available in various comb configurations:

Ordering

Ordering

items

Use the filters below to refine results!

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1610180
Starter kit for hand casting 7.5% TGX Stain-Free polyacrylamide gels, includes acrylamide solutions and buffers
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1610181
Kit for hand casting 7.5% TGX Stain-Free polyacrylamide gels, includes acrylamide solutions and buffers
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1610182
Starter kit for hand casting 10% TGX Stain-Free polyacrylamide gels, includes acrylamide solutions and buffers
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1610183
Kit for hand casting 10% TGX Stain-Free polyacrylamide gels, includes acrylamide solutions and buffers
tgx-stain-free-fastcast-acrylamide-starter-kit-sku-130-0184-thumb.jpg
1610184
Starter kit for hand casting 12% TGX Stain-Free polyacrylamide gels, includes acrylamide solutions and buffers
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1610185
Kit for hand casting 12% TGX Stain-Free polyacrylamide gels, includes acrylamide solutions and buffers

Accessories

Accessories

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1658000FC
4-gel vertical electrophoresis system, 0.75 mm gel thickness, includes companion module, 2 casting stands, 4 casting frames, 10-well combs, 5 short plates, and 5 spacer plates, with TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10% (161-0182)
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1658001FC
4-gel vertical electrophoresis system, 1.0 mm gel thickness, includes companion module, 2 casting stands, 4 casting frames, 10-well combs, 5 short plates, and 5 spacer plates, with TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10% (161-0182)
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1658006FC
4-gel vertical electrophoresis system, 1.5 mm gel thickness, includes companion module, 2 casting stands, 4 casting frames, 10-well combs, 5 short plates, and 5 spacer plates, with TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10% (161-0182)

Kits & Reagents

Kits & Reagents

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1610700
10 g, ammonium persulfate (APS) catalyst
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1610737
30 ml, premixed protein sample buffer for SDS-PAGE
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1610747
10 ml, premixed 4x Laemmli protein sample buffer for SDS-PAGE
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(Discontinued) 10x Tris/Glycine/SDS
1610772
Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water
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(Discontinued) TEMED
1610800
5 ml, for catalyst for polyacrylamide gels
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(Discontinued) TEMED
1610801
50 ml, catalyst for polyacrylamide gels; hazardous material shipping charges may apply

Standards

Standards

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1610393
2.5 ml (5 x 500 µl), mixture of ten blue-stained recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 250 applications
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1610394
2.5 ml (5 x 500 µl), mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 250 applications
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1610395
2.5 ml (5 x 500 µl), mixture of ten multicolor recombinant proteins (10–250 kD), 250 applications
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1610397
2.5 ml (5 x 500 µl), mixture of 12 recombinant proteins (2–250 kD), nine blue-stained bands, and three pink reference bands (2, 25, 75 kD), 250 applications
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1610396
5 ml (5 x 1 ml), unstained mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 500 applications

Plates

Plates

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1653308
Pkg of 5, short plate for Mini-PROTEAN Tetra System and Mini-PROTEAN 3 Multi-Casting Chamber
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1653310
Pkg of 5, spacer plate with 0.75 mm integrated spacer, for Mini-PROTEAN Tetra System and Mini-PROTEAN 3 Multi-Casting Chamber
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1653311
Pkg of 5, spacer plate with 1.0 mm integrated spacer, for Mini-PROTEAN Tetra System and Mini-PROTEAN 3 Multi-Casting Chamber
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1653312
Pkg of 5, spacer plate with 1.5 mm integrated spacer, for Mini-PROTEAN Tetra System and Mini-PROTEAN 3 Multi-Casting Chamber
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4560001
Pkg of 50, cassettes with IPG well marks for casting gels 8.6 x 7.2 cm by 1.0 mm thick, for use with Mini-PROTEAN Electrophoresis Cells; purchase combs separately (456-0011)
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4560003
Pkg of 50, cassettes with 10 well marks for casting gels 8.6 x 7.2 cm by 1.0 mm thick, for use with Mini-PROTEAN Electrophoresis Cells; purchase combs separately (456-0013)
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4560005
Pkg of 50, cassettes with 12 well marks for casting gels 8.6 x 7.2 cm by 1.0 mm thick, for use with Mini-PROTEAN Electrophoresis Cells; purchase combs separately (456-0015)
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4560006
Pkg of 50, cassettes with 15 well marks for casting gels 8.6 x 7.2 cm by 1.0 mm thick, for use with Mini-PROTEAN Electrophoresis Cells; purchase combs separately (456-0015)

Documents

Documents

Number Description Options
10042306
How to Cast Your Gels Fast — TGX™ FastCast™ Acrylamide Solutions Quick Guide, Rev A
6545
Bio-Rad’s TGX and TGX Stain-Free FastCast Acrylamide Kits: Tips & Tricks, Rev A
6533
Introducing Bio-Rad’s TGX and TGX Stain-Free FastCast Acrylamide Kits, Rev A
10033918
Instruction Manual, TGX™ and TGX Stain-Free™ FastCast™ Acrylamide Kit and Starter Kit
6455
FastCast Acrylamide Kits and Handcasting Equipment Brochure
6502
TGX and TGX Stain-Free FastCast Acrylamide Kits Brochure, Rev B
[ Add to Cart (Free) ]
10040758
Instruction Manual, Image Lab™ Software: Imaging Stain-Free Gels and Blots, Rev B
6507
TGX and TGX Stain-Free Gels: Publications List
10040758
Instruction Manual, Image Lab™ Software: Imaging Stain-Free Gels and Blots, Rev B
0512
Evaluation of Stain-Free Gels for Gel-MS Applications (Poster)
6008
Comparison of the Criterion TGX Stain-Free Precast Gel System and Standard Coomassie Staining Procedures for Running and Imaging Protein Gels
2317
Ready-to-Run Buffers and Solutions Brochure, Rev F
RP0058
A Defined Methodology for Reliable Quantification of Western Blot Data
6494
Protein Purification Workflow with the NGC Chromatography System and Stain-Free Gels Brochure
[ Add to Cart (Free) ]