Gel Doc XR+ Gel Documentation System

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Gel Doc XR System

Overview

DNA Stains
DNA Stains

Bio-Rad offers a wide selection of products for a variety of gel detection applications. For nucleic acid detection, fluorescent stains are available for both DNA and RNA detection.

Precast Agarose Gels

ReadyAgarose™ gels are individually packaged precast agarose gels. The UV-transparent tray backing each ReadyAgarose gel locks into the Mini-Sub Cell GT and ReadySub-Cell GT electrophoresis chambers. These gels provide resolution of nucleic acids from 20 to 10,000 base pairs.

Precast Agarose Gels

Precast Agarose Gel Features

  •  
    • UV-transparent tray with fluorescent lane numbers and ruler
    • Tray locks into Bio-Rad electrophoresis cell of corresponding size
    • Mini 7 x 10 cm and wide mini 15 x 10 cm formats
    • Made with TAE or TBE buffer
    • Gel percentages for optimal resolution:
      1.0%, 200 bp–10 kb
      3.0%, 20 bp–1 kb
    • Shipment and storage at room temperature
    • One-year shelf life
DNA Electrophoresis Size Standards

EZ Load standards for DNA. Bio-Rad molecular rulers are available in several convenient size ranges and increments for sizing single- and double-stranded DNA. Visually distinct references bands make it easy to determine the size of your sample DNA.

Imaging System Selection Guide

  ChemiDoc MP ChemiDoc Touch GelDoc GO Gel Doc XR+ GS-900
ChemiDoc
MP
ChemiDoc
GelDoc Go
Gel Doc
XRS+
GS-900
Nucleic Acid Detection
Ethidium Bromide
SYBR Green I and SYBR Safe Dyes
Fast Blast DNA Stain
Protein Staining, 1-D Gels
Stain-Free Gels
Coomassie Blue Stain
Silver Stain
SYPRO Ruby, Flamingo, and Oriole Gel Stains
Protein Staining, 2-D Gels
Coomassie Blue Stain
Silver Stain
SYPRO Ruby, Flamingo, and Oriole Gel Stains
Cy2, Cy3, Cy5 Label
Blot Detection
Multiplex fluorescence
Immun-Star 
Chemiluminescence
Stain-free blots
Colorimetric blots
SYPRO Ruby Protein Blot Stain*

*  Optimal with low fluorescence PVDF membrane.

The Gel Doc XR+ System is a fast, easy-to-use, completely automated gel imaging and documentation system controlled by Image Lab Image Capture and Analysis Software.


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Ordering Information


Description

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What is Stain-Free Technology?

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The Gel Doc XR+ System is based on CCD high-resolution, high-sensitivity detection technology and modular options to accommodate a wide range of samples and support multiple detection methods including fluorescence and colorimetric detection. The Gel Doc XR+ System is controlled by Image Lab Software to optimize imager performance for fast, integrated, and automated image capture and analysis of various samples.

The system accommodates a wide array of samples, from large handcast polyacrylamide gels to small ReadyAgarose Gels and various blots. The system is an ideal accompaniment to PCR, purification, and electrophoresis systems, enabling image analysis and documentation of restriction digests, amplified nucleic acids, genetic fingerprinting, RFLPs, and protein purification and characterization.

Features and Benefits of the Gel Doc XR+ System

  • Gel and blot imaging and analysis are quick and accurate
  • Automated, hands-off routines; no training is required
  • Save and recall all the steps in the workflow for repeatable and reproducible results
  • Optimize the system at setup for image data that is always accurate, reproducible, and free of imaging artifacts
  • Wide range of applications with special accessories to preserve sample integrity for downstream research while ensuring user safety
  • Comprehensive, automated quantitative analysis of protein and DNA samples in seconds
  • Customize and organize data in reports
  • Obtain publication-quality results quickly

The Gel Doc XR+ System optimizes reproducibility and reliability of experimental data, enabling quantitative comparisons between different experiments. Using proprietary algorithms, this imaging system is calibrated at setup to ensure:

  • Images are in focus at all times, regardless of zoom level or sample position
  • Appropriate flat fielding correction is automatically and consistently applied to image data for every application
  • Imaging artifacts are automatically corrected

The Gel Doc XR+ System enables quick and easy visualization, documentation, and analysis of nucleic acid and protein gels, blots, and macroarrays with a few clicks of the mouse. The system supports fluorescence and colorimetric detection methods. The Gel Doc XR+ System consists of a darkroom hood, CCD camera and software-controlled motorized lens, UV and white light illuminators, filter slider with standard filter, and UV-protection shield. The system enables you to:

  • Increase cloning efficiency and protein production by protecting DNA electrophoresis samples from UV exposure using the XcitaBlue conversion screen and blue light excitable stains such as GelGreen, SYBR Safe, and SYBR Green I
  • Maintain standard operating procedures or criteria for sample performance as there is no loss in sensitivity compared to UV and ethidium bromide staining
  • For imaging SYBR Safe DNA applications, you may use the UV transilluminator or the optional XcitaBlue kit. The XcitaBlue (UV to blue conversion screen) protects samples from UV damage and also allows you to externally visualize DNA samples without a UV shield

The Gel Doc XR+ System can be upgraded to the ChemiDoc XRS+ System.


Specifications

Applications  
Chemiluminescence No
Fluorescence* Yes
Colorimetry/densitometry Yes
Gel documentation Yes
Hardware Specifications  
Maximum sample size 28 x 36 cm
Maximum image area 19.4 x 26 cm
Excitation source Epi-white light and trans-UV (302 nm) are standard (optional 365 nm lamp available); optional trans-white conversion screen and XcitaBlue™ UV/blue conversion screen available
Illumination control 3 modes (trans-UV, trans white, epi-white)
Detector CCD
Image resolution 4 megapixels
Pixel size (H x V) 4.65 x 4.65 µm
Filter holder 3 positions (2 for filters, 1 without filter)
Emission filters 1 included (standard), 3 optional
Dynamic range >3.0 orders of magnitude
Pixel density (gray levels) 4,096
Dynamic flat fielding Application specific, for all applications
Instrument size (L x W x H) 36 x 60 x 96 cm
Instrument weight 32 kg
Operating Ranges  
Operating voltage 110/115/230 V AC nominal
Operating temperature 10–28°C (21°C recommended)
Operating humidity <70% noncondensing
Automation Capabilities  
Workflow automated selection Application driven; user selected or recalled by a protocol
Workflow automated execution Controlled by a protocol via application specific setup for image area, illumination source, filter, analysis, and reporting
Workflow reproducibility 100% repeatability via recallable protocols; from image capture to quantitative analysis and reports
Autofocus (patent pending) Precalibrated focus for any zoom setting or sample height
Image flat fielding** Dynamic; precalibrated and optimized per application
Autoexposure 2 user-defined modes (intense or faint bands)
*Use the optional XcitaBlue kit if performing SYBR® Safe DNA applications, because the UV to blue conversion screen allows you to visualize DNA samples while protecting against UV damage.

**U. S. patent 5,951,838.
 

Ordering

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Molecular Imager Gel Doc XR+ System with Image Lab Software

1708195
Gel imaging system, includes darkroom, UV transilluminator, epi-white illumination, camera, cables, Image Lab software compatible with Windows or Mac OS

This product is being discontinued soon. Please contact customer service for more information.

Accessories

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list price. If the problem persists, please call customer service at or send an
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Image Lab™ Upgrade for Gel Doc™ XR+ System

1708199
Upgrade package for Gel Doc XR+ system includes Image Lab software, focus calibration target, flat fielding disk, installation guide, and user manual

This product is being discontinued soon. Please contact customer service for more information.
(Discontinued) Image Lab™ Software

1709690
Software, PC or Mac, for automated image capture, optimization, and 1-D data analysis, for use with Gel Doc™ EZ, Gel Doc XR+, ChemiDoc™ XRS+, ChemiDoc MP, and GS-900™ imaging systems

This product is no longer available. Please contact technical support for more information on replacement product.

The following replacement is recommended:

  • Image Lab Software on USB drive (12012931)
Focus Calibration Target

1708026
Pkg of 1, calibration target for Gel Doc™ XR+, ChemiDoc™ XRS+, and ChemiDoc MP imaging systems with Image Lab™ software

List Price: 
 
$100.00 
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UV Safety Shield for ChemiDoc™ XRS+ and GelDoc™ XR+ Systems

1708279
Pkg of 1, UV safety shield to protect users from UV light exposure during band excision; for use with all ChemiDoc and GelDoc Imaging Systems from the universal hood series

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$236.00 
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Standard Emission Filter Replacement

1708081
Filter, standard emission, 62 mm for Gel Doc™ XR+ and ChemiDoc™ XRS+ systems

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$476.00 
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White Light Conversion Screen

1708289
Pkg of 1, conversion screen for viewing Coomassie, silver stain, and other colorimetric gel stains; for use with non touch–screen ChemiDoc™ MP, ChemiDoc™ XRS+, and Gel Doc™ XR+ Systems

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$647.00 
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XcitaBlue™ Conversion Screen

1708182
Pkg of 1, conversion screen for viewing SYBR® Green, SYBR® Safe, GFP, and Flamingo™ Fluorescent Gel Stains, includes viewing goggles; for use with ChemiDoc™ XRS+, non touch–screen ChemiDoc MP, and Gel Doc™ XR+ Systems

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$1,481.00 
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Fluorescent Reference Plate

1708008
Pkg of 1, accessory for flat fielding

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$141.00 
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Gel Alignment Template Kit

1708184
Pkg of 3 gel templates, for use with ChemiDoc™ XRS+, ChemiDoc MP, and Gel Doc™ XR+ Systems, includes template holder

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$57.00 
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XcitaBlue Viewing Goggles

1708185
Pkg of 1, goggles for use with SYBR Safe dyes and Blue Trays for use with ChemiDoc and GelDoc Imaging Systems

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$28.00 
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Mitsubishi Thermal Printer

1708089
Optional thermal printer, for use with Bio-Rad imaging systems

List Price: 
 
$1,992.00 
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Fluorescent Ruler

1703759
Pkg of 1, accessory, for use with ChemiDoc™ XRS+, ChemiDoc MP, and Gel Doc™ XR+ systems

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$25.00 
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Gel Cutter

1703760
Pkg of 1, accessory, for use with ChemiDoc and Gel Doc Imaging Systems

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$25.00 
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Standard 302 nm UV Lamps

1708097
Pkg of 6, 302 nm replacement UV lamps for all ChemiDoc™ and Gel Doc™ Imaging Systems

List Price: 
 
$396.00 
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365 nm UV Lamps

1706887
Pkg of 6, replacement lamp for Gel Doc™ XR, ChemiDoc™ XRS, and ChemiDoc MP systems

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$344.00 
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Filter

1708077
Pkg of 1, filter, 480BP70 62 mm, for Hoechst/coumarin, for use with Gel Doc XR and ChemiDoc XRS systems

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$947.00 
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Documents

Number Description Options
7051 Imaging Systems Family Flier Click to download [ Add to Cart (Free) ]
5838 Gel Doc™ XR+ Imaging System Brochure, Rev G Click to download [ Add to Cart (Free) ]
2577 Instruction Manual, Molecular Imager Gel Doc XR and ChemiDoc XRS Systems Installation Guide, Rev B Click to download
10014084 Instruction Manual, GelDoc XR and ChemiDoc XRS, Rev A Click to download
10000076955 Gel Doc™ XR+ and ChemiDoc™ XRS+ Imaging Systems with Image Lab™ Software Instrument Guide Click to download
MI1708124 Instruction Manual, White Light Transilluminator, Rev A Click to download
10016116 Gel Alignment Template Kit Click to download
4000177 Instruction Manual, IPD Multi-Color Imaging Target, Rev A Click to download
10040758 Instruction Manual, Image Lab™ Software: Imaging Stain-Free Gels and Blots, Rev B Click to download
6434 Western Blot Normalization Using Image Lab™ Software, Quick Start Guide Click to download [ Add to Cart (Free) ]
6427 Bio-Rad Stain-Free Western Blotting Click to download [ Add to Cart (Free) ]
2589 Not All Bits Are Created Equal Click to download

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