Precision Plus Protein™ Standard Plugs

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Precision Plus Protein™ Standard Plugs

Overview

Using Precision Plus Protein™ Standard Plugs

Step 1
Break and twist off one end of the plastic plug casing.

Step 2
Break off an individual plastic plug from the casing by placing pressure on the flat side to break the junction.

Step 3
Complete the break by twisting off the individual plastic plug casing.

Step 4
Snap off the attached spatula.

Step 5
Use the spatula to push the plug out of the plastic casing.

Step 6
Use the spatula to slide the plug outward and on to the top plate of your gel.

Step 7
Slide the plug all the way down the bottom of the well next to IPG strip. Then add an agarose overlay.



Second dimension SDS-PAGE gel run with a mouse brain sample, Precision Plus Standards Plugs, and stained with Bio-Safe™ coomassie G-250 stain.

Unstained Standards

Precision Plus Protein unstained standards on a gel. These standards have been engineered to contain the unique Strep-tag affinity peptide, which allows detection and molecular weight determination on western blots.

Precision Plus Protein™ Standard Plugs

Precision Plus Protein standards packaged in an easy-to-use plug for 2-D electrophoresis.

Precision Plus Protein™ Standard Plug Run on a PROTEAN Plus Gel

Precision Plus Protein Standard plug on a 12% PROTEAN Plus precast gel. Sample is mouse liver.

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Precision Plus Protein standard plugs allow easy, quick, and clean loading of molecular weight standards on any gel. The plugs can be used on vertical 2-D gels with no reference well. Precision Plus Protein unstained standards are cast in 1 mm thick agarose plugs for easy storage, handling, and loading. Load concentrations have been optimized for SYPRO Ruby, Silver Stain Plus™, and Bio-Safe™ Coomassie Blue staining.

Some features and benefits of the plugs are:

  • Precast for ease of use — just snap, twist, and load the plug onto a gel
  • Absolute molecular weight accuracy, confirmed by mass spectrometry
  • Ideal ten-band range from 10 kD to 250 kD, with 3 high-intensity reference bands (25 kD, 50 kD, and 75 kD) for instant gel orientation
  • Molecular weights that never change, so band sizes are easy to remember
  • High r2 value, >0.995, allowing accurate molecular weight determination
  • Known concentration of protein in each band, allowing quantitation capability
  • Strep-tag amino acid sequence for detection and molecular weight determination on western blots

 

Precision Plus Protein standard plugs come in easy-to-use snap-off molds in packs of 24 (one application per plug).

MW size range 10 kD – 250 kD
Stain type Unstained
Volume 24 Plugs
Number of applications 24
Plug buffer 1% agarose, 128 mM glycine,
10% (w/v) glycerol,
0.7% SDS, 62.5 mM Tris pH8.3,
50 mM DTT, 5 mM EDTA,
0.02% NaN3,
0.003% bromophenol blue
Shelf life at 4°C, months 3
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Precision Plus Protein™ Standard Plugs, unstained, Strep-tagged, recombinant

1610378
Pkg of 24, 1 mm thick agarose plugs containing ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands

0 1610378 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0378_0921120327264.xml 1610378 /default/main/bio-rad/WORKAREA/default 09/21/09 12:03 PM en_US juso9m8o 2018-02-01 00:22:43 true true internet/product_SKU juso9m8o templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0378_0921120327264.xml LSR 2019-05-04 18:49:31 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1556922622287 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 18:49:31 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0378_0921120327264.xml templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0378_0921120327264.xml 1610378-precision-plus-protein-standard-plugs-unstained-strep-tagged-recombinant 1610378 Precision Plus Protein Standard Plugs Precision Plus Protein&trade; Standard Plugs, unstained, <em>Strep</em>-tagged, recombinant Pkg of 24, 1 mm thick agarose plugs containing ten <em>Strep</em>-tagged, recombinant proteins (10–250 kD), including three reference bands Pkg of 24, 1 mm thick agarose plugs containing 10 Strep-tagged recombinant proteins (10–250 kD), including three reference bands /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/161-0378_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/161-0378_view.jpg Precision Plus Protein&trade; Standard Plugs ,VN RepEx Precision Plus Protein&trade; Standard Plugs Pkg of 24, 1 mm thick agarose plugs containing 10 Strep-tagged recombinant proteins (10–250 kD), including three reference bands 1610378, precision plus protein standard plugs, unstained, strep-tagged, strep-tag, recombinant, strep, streptavidin, tagged, standard, protein, markers, marker, molecular, weight, mw, ladders, ladder 09/21/09 12:03 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <p>Use Precision Plus Protein Standard Plugs for second-dimension gels in proteomics studies and for high-throughput electrophoresis. The unique <em>Strep</em>-tag affinity peptide allows detection and molecular weight determination on western blots.<br /></p> <ul> <li>Package of 24, 1 mm thick agarose plugs</li> <li>Mixture of ten <em>Strep</em>-tagged, recombinant proteins (10&ndash;250 kD)</li> <li>Includes three reference bands (25, 50, and 75 kD)</li> </ul> <h2 class="descHeader">Related Products<br /></h2> <ul> <li>Precision Plus Protein Unstained Standards, Strep-tagged, recombinant, 1 ml (<a href="/en-us/sku/1610363-precision-plus-protein-unstained-standards-strep-tagged-recombinant?ID=1610363">1610363</a>)</li> <li>Precision Plus Protein Unstained Standards Value Pack, Strep-tagged, recombinant, 5 ml (5 x 1 ml) (<a href="/en-us/sku/1610396-precision-plus-protein-unstained-standards-value-pack-strep-tagged-recombinant?ID=1610396">1610396</a>)</li> </ul> <p>For blot development, Precision Protein&trade; StrepTactin-HRP Conjugate or StrepTactin-AP Conjugate should also be ordered.</p> <ul> <li>Precision Protein StrepTactin-HRP Conjugate, 300 &micro;l (<a href="/en-us/sku/1610380-precision-protein-streptactin-hrp-conjugate-300-mul?ID=1610380">1610380</a>)</li> <li>Precision Protein StrepTactin-HRP Conjugate, 125 &micro;l (<a href="/en-us/sku/1610381-precision-protein-streptactin-hrp-conjugate-125-mul?ID=1610381">1610381</a>)</li> <li>StrepTactin-AP Conjugate, 300 &micro;l (<a href="/en-us/sku/1610382-precision-protein-streptactin-ap-conjugate-300-mul?ID=1610382">1610382</a>)</li> </ul> <p>More Precision Plus Protein Standards:</p> <ul> <li><a href="/en-us/product/unstained-protein-standards?ID=ab429c02-ab15-4967-a7b9-1f78850fc4d3">Precision Plus Protein Unstained Standards</a> </li> <li><a href="/en-us/product/prestained-protein-standards?ID=a7b0f9ce-e080-4b51-ab99-4cded66497c1">Precision Plus Protein Prestained Standards</a></li> <li><a href="/en-us/product/western-blotting-protein-standards?ID=1d045ed2-e2d3-4c21-9d1c-a956dbaf6b21">Precision Plus Protein&trade; WesternC&trade; Standards</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <p><a href="/en-us/category/protein-ladders-standards-markers">Protein Ladders and Standards</a><br /> <a href="/en-us/category/protein-gels">Precast Protein Gels</a><br /><a href="/en-us/category/protein-electrophoresis-blotting">Protein Electrophoresis and Blotting Products</a></p> <h2 class="descHeader">Supporting Documents and Resources</h2> <ul> <li>Precision Plus Protein Unstained Standard Plugs <a href="/webroot/web/pdf/lsr/literature/Bulletin_4117684.pdf" target="_blank">Instruction Manual</a></li> <li>Unstained Precision Plus Protein Standards <a href="/webroot/web/pdf/lsr/literature/Bulletin_4110023.pdf" target="_blank">Instruction Manual</a></li> <li><em>Strep</em>-Tag Technology for Molecular Weight (MW) Determination on Blots Using Precision Plus Protein Standards <a href="/webroot/web/pdf/lsr/literature/Bulletin_2847.pdf" target="_blank">Application Note</a></li> </ul> <ul style="padding-left: 30px;"> </ul> <h2 class="descHeader">Related Applications</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-protein-electrophoresis?ID=LUSOVO47B">Protein Electrophoresis</a></li> <li><a href="/en-us/applications-technologies/introduction-western-blotting?ID=LUSPPAKG4">Western Blotting</a></li> </ul> <ul style="padding-left: 30px;"> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated/ 161-0378_0921120327264_en.html /webroot/web/html/lsr/products/SKUs/autopopulated//161-0378_0921120327264_en.html MW size range, kD 1859 10&ndash;250 1 0 Stain type 2195 Unstained 1 0 Volume, &micro;l 2321 N/A 0 0 Total number of applications 2274 24 1 0 Shelf life at 4&deg;C, months 2151 3 1 0 Loading volume on mini gels, &micro;l 1793 N/A 0 0 <b>Application Type</b> 1130 &nbsp; 0 0 Monitoring electrophoretic separation for SDS-PAGE gels 2733 N/A 0 0 Molecular weight sizing on SDS-PAGE gels 2734 Yes 0 0 Monitoring blot transfer efficiency 2735 N/A 0 0 Fluorescence blots 1649 N/A 0 0 Molecular weight sizing on 2736 Stain-free gels, 2-D gels 0 0 <b>Loading Volume on Mini Gels</b> 1142 &nbsp; 0 0 For Coomassie staining, &micro;l 1658 N/A 0 0 For stain-free gels, &micro;l 2737 N/A 0 0 For silver staining, &micro;l 2738 N/A 0 0 For SYPRO Ruby staining, &micro;l 2739 N/A 0 0 For blot development, &micro;l 1657 N/A 0 0 StrepTactin conjugate working dilutions for colorimetric blot development 2218 N/A 0 0 StrepTactin conjugate working dilutions for chemiluminescence blot development 2217 N/A 0 0 5671101 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/567-1101_1299190198.xml 1632014 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/163-2014_0921120355715.xml 1632105 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/163-2105_0921120349601.xml false $137.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610378&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610378&CartQuote=QT&quantity= 1205 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610378&AddToDefaultWishList=true&quantity= 41105300 137.00 Product detail page 1610378
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Number Description Options
6533 Introducing Bio-Rad’s TGX and TGX Stain-Free FastCast Acrylamide Kits, Rev A Click to download
2414 The Little Book of Standards, Rev D Click to download
2998 Protein Standards Application Guide, Rev G Click to download [ Add to Cart (Free) ]
3036 Precision Plus Protein Plugs Promotional Card, Rev C Click to download
4117684 Instruction Manual, Precision Plus Protein™ Standard Plugs, Unstained, Rev C Click to download
6493 Bio-Rad’s Host Cell Protein (HCP) Workflow Quick Guide Click to download [ Add to Cart (Free) ]

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