2-D Electrophoresis Workflow

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Overview

The 2-D Electrophoresis Workflow provides a comprehensive selection of product solutions and educational resources that guide you to better 2-D electrophoresis results.

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Description

Description

Bio-Rad's 2-D Electrophoresis Workflow provides a comprehensive selection of product solutions and educational resources that guide you to better 2-D results.

4 Steps

  1. Sample Preparation — sample preparation is the first step toward optimizing your results. Bio-Rad offers easy-to-use kits: ReadyPrep™ 2-D starter kit, ReadyPrep 2-D cleanup kit, and ProteoMiner™ protein enrichment kit for getting started and for cleanup and enrichment of low-abundance proteins.
  2. First Dimension Electrophoresis — the first dimension of 2-D electrophoresis is separation based on a protein's isoelectric point. Bio-Rad's IPG strips offer many pH gradient options and strict quality control. The award-winning PROTEAN® i12™ IEF system gives you the flexibility to run multiple pH gradients, protocols, and samples simultaneously.
  3. Second Dimension Electrophoresis — the second dimension of 2-D electrophoresis is separation based on molecular weight. Bio-Rad's Criterion™ system, Criterion precast gels, Criterion™ Dodeca™ cell, and Precision Plus Protein™ standard plugs provide the confidence of a leakproof precast gel and the ability to run up to 12 gels at once.
  4. Staining, Imaging, and Analysis — staining, imaging, and analysis allow you to document and evaluate your 2-D results. With Bio-Rad's sensitive and hassle-free Oriole™ gel stain and versatile ChemiDoc™ MP imager, you can obtain high-quality images. Analyze those images with PDQuest™ software, a highly powerful tool for 2-D evaluation.

2-D Workflow

Applications and Technical Resources

The 2-D electrophoresis applications and technology page is a virtual encyclopedia of 2-D electrophoresis. You'll find extensive information, videos, and troubleshooting guides to ensure you get the best 2-D results possible.

On the 2-D Electrophoresis applications and technology page you can:

Ordering

Ordering

items

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1646000
Isoelectric focusing system, includes basic unit, electrode assemblies, focusing and rehydration/equilibration trays, forceps, electrode wicks, mineral oil, cleaning accessories, IPG strips and sample/rehydration buffer, USB flash drives, styluses
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1708280
This model of the ChemiDoc MP Imaging System has been replaced by the new ChemiDoc MP Imager.
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1654139
Vertical midi-format electrophoresis cell (#165-4130), 12 gel capacity, includes buffer tank with cooling coil, lid with power cables, universal power supply (#164-5070)

Accessories

Accessories

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1708289
Pkg of 1, conversion screen for viewing Coomassie, silver stain, and other colorimetric gel stains; for use with non touch–screen ChemiDoc™ MP, ChemiDoc™ XRS+, and Gel Doc™ XR+ Systems

Kits & Reagents

Kits & Reagents

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1610378
Pkg of 24, 1 mm thick agarose plugs containing ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands
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1633006
10 preps, low-abundance protein enrichment kit for 10 mg of total protein, includes 10 spin columns, wash buffer, elution reagents, collection tubes
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1633007
10 preps, low-abundance protein enrichment kit for 50 mg of total protein, includes 10 spin columns, wash buffer, elution reagents, collection tubes
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1632130
50 preps, includes 2 precipitation agents, 2 wash reagents, 1 wash reagent additive; for 50 x 100 µl protein samples each containing up to 500 µg protein
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1632105
2-D gel electrophoresis kit, includes protein sample and buffers for IPG strip rehydration, focusing, and transfer to second dimension (IPG strips not included)

Gels

Gels

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5671071
Pkg of 1, 18% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671081

Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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5671091

Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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5671101
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671111
Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671121

Pkg of 1, AnykD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

IPG Strips

IPG Strips

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1632014
Pkg of 12, 11 cm, pH 3–10, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632015
Pkg of 12, 11 cm, pH 4–7, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632016
Pkg of 12, 11 cm, pH 3–10 nonlinear, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632017
Pkg of 12, 11 cm, pH 3–6, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632018
Pkg of 12, 11 cm, pH 5–8, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632019
Pkg of 12, 11 cm, pH 7–10, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632024
Pkg of 12, 11 cm, pH 3.9–5.1, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632026
Pkg of 12, 11 cm, pH 5.5–6.7, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632027
Pkg of 12, 11 cm, pH 6.3–8.3, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems

Documents

Documents

Number Description Options
6478
2-D Electrophoresis Workflow Brochure, Rev B
[ Add to Cart (Free) ]
6493
Bio-Rad’s Host Cell Protein (HCP) Workflow Quick Guide
[ Add to Cart (Free) ]
2651
2-D Electrophoresis Workflow How-To Guide, Rev F
5635
ProteoMiner Protein Enrichment Technology Bulletin, Rev B
10010636
Instruction Manual, ProteoMiner Protein Enrichment Kits, Rev D
4110143
Instruction Manual, ReadyPrep 2-D Cleanup Kit, Rev A
4110009
Instruction Manual, ReadyPrep 2-D Starter Kit, Rev A
2642
ReadyPrep 2-D Starter Kit Product Information Sheet
6097
PROTEAN i12 IEF System Brochure, Ver B
[ Add to Cart (Free) ]
6138
PROTEAN i12 IEF System: Independent Voltage and Current Control Enables Optimization of First-Dimension IEF Conditions, Rev A
4006166
Instruction Manual, ReadyStrip IPG Strip
2442
ReadyStrip IPG Strip Product Information Sheet, Rev F
4006197
Instruction Manual, Criterion Dodeca Cell, Rev A
6040
Electrophoresis Guide, Interactive PDF, Rev C
4110001
Instruction Manual, Criterion™ Gel Application Guide, Ver H
5991
Oriole Fluorescent Gel Stain: A Novel Fluorescent Stain for a Simpler 2-D PAGE to MALDI Workflow, Rev A
5900
Oriole Fluorescent Gel Stain PIS, Rev A
6427
Bio-Rad Stain-Free Western Blotting
[ Add to Cart (Free) ]
3121
PDQuest 2-D Analysis Software Brochure, Rev C
3194
EXQuest Spot Cutter Brochure, Rev A
10001531
Instruction Manual, EXQuest™ Spot Cutter, Rev A
6371
Electrophoresis Power Supplies Brochure, Rev C
[ Add to Cart (Free) ]
6393
Reliable, Streamlined 2-D Western Blot Workflow for Evaluation of Antibodies Developed for Detection of Host Cell Proteins, Rev A
[ Add to Cart (Free) ]
6405
Effectiveness of 2-D Electrophoresis over 1-D Electrophoresis Followed by Western Blotting for Evaluating Strategies Used to Generate Host Cell Protein Antibodies, Rev A