2-D Electrophoresis Workflow

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2-D Electrophoresis Workflow

Overview

2-D Electrophoresis Workflow How-To Guide

2-D Electrophoresis Workflow How-To Guide

Guides You Through 2-D Electrophoresis From Start to Finish

This manual provides in-depth information about:

  • Experimental methods and tools
  • Standard protocols
  • Ideas for developing customized protocols
  • Tips and tricks to improve results and reproducibility

Download PDF

2-D Doctor

Troubleshooting 2-D Electrophoresis Gels with 2-D Doctor™

The 2-D Doctor is a self-help guide developed by Bio-Rad that enables you to identify and troubleshoot your 2-D gel issues. Comprehensive solutions and suggestions are provided to help solve 2-D gel electrophoresis challenges.

Learn more

PROTEAN® i12™ IEF System Tour

12 Lanes. Individual Control. Total Confidence.

 

Simultaneously run 12 lanes, each with different samples, pH gradients, and protocols

  • Optimize experiments in fewer runs
  • Run multiple samples at once
  • Get more reproducible results

Learn how to:

  • Assemble trays, electrodes, and
    load samples
  • Use the easy touch-screen control to program and operate the system
  • Use the free web-based application for data analysis and protocol design

Launch the System Tour

The 2-D Electrophoresis Workflow provides a comprehensive selection of product solutions and educational resources that guide you to better 2-D electrophoresis results.


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Ordering Information


  • Description
  • Ordering
  • Accessories
  • Kits & Reagents
  • Gels
  • IPG Strips
  • Documents

Description

Bio-Rad's 2-D Electrophoresis Workflow provides a comprehensive selection of product solutions and educational resources that guide you to better 2-D results.

4 Steps

  1. Sample Preparation — sample preparation is the first step toward optimizing your results. Bio-Rad offers easy-to-use kits: ReadyPrep™ 2-D starter kit, ReadyPrep 2-D cleanup kit, and ProteoMiner™ protein enrichment kit for getting started and for cleanup and enrichment of low-abundance proteins.
  2. First Dimension Electrophoresis — the first dimension of 2-D electrophoresis is separation based on a protein's isoelectric point. Bio-Rad's IPG strips offer many pH gradient options and strict quality control. The award-winning PROTEAN® i12™ IEF system gives you the flexibility to run multiple pH gradients, protocols, and samples simultaneously.
  3. Second Dimension Electrophoresis — the second dimension of 2-D electrophoresis is separation based on molecular weight. Bio-Rad's Criterion™ system, Criterion precast gels, Criterion™ Dodeca™ cell, and Precision Plus Protein™ standard plugs provide the confidence of a leakproof precast gel and the ability to run up to 12 gels at once.
  4. Staining, Imaging, and Analysis — staining, imaging, and analysis allow you to document and evaluate your 2-D results. With Bio-Rad's sensitive and hassle-free Oriole™ gel stain and versatile ChemiDoc™ MP imager, you can obtain high-quality images. Analyze those images with PDQuest™ software, a highly powerful tool for 2-D evaluation.

2-D Workflow

Applications and Technical Resources

The 2-D electrophoresis applications and technology page is a virtual encyclopedia of 2-D electrophoresis. You'll find extensive information, videos, and troubleshooting guides to ensure you get the best 2-D results possible.

On the 2-D Electrophoresis applications and technology page you can:


Ordering

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PROTEAN® i12™ IEF system

1646000
Isoelectric focusing system, includes basic unit, electrode assemblies, focusing and rehydration/equilibration trays, forceps, electrode wicks, mineral oil, cleaning accessories, IPG strips and sample/rehydration buffer, USB flash drives, styluses

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Criterion™ Dodeca™ Cell and PowerPac™ Universal Power Supply

1654139
Vertical midi-format electrophoresis cell (#165-4130), 12 gel capacity, includes buffer tank with cooling coil, lid with power cables, universal power supply (#164-5070)

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$6,214.00 
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PDQuest Advanced 2-D Analysis Software

1709630
1-user license, 2-D analysis software, provides advanced functionality

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Accessories

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Criterion Staining/Blotting Trays

3459920
Pkg of 12, gel staining trays, with lids

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$253.00 
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White Light Conversion Screen

1708289
Pkg of 1, conversion screen for viewing Coomassie, silver stain, and other colorimetric gel stains; for use with non touch–screen ChemiDoc™ MP, ChemiDoc™ XRS+, and Gel Doc™ XR+ Systems

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$647.00 
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Kits & Reagents

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Precision Plus Protein™ Standard Plugs, unstained, Strep-tagged, recombinant

1610378
Pkg of 24, 1 mm thick agarose plugs containing ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands

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$141.00 
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Oriole Fluorescent Gel Stain

1610496
1 L, 1x Oriole fluorescent gel stain solution

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$214.00 
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ProteoMiner™ Protein Enrichment Small-Capacity Kit

1633006
10 preps, low-abundance protein enrichment kit for 10 mg of total protein, includes 10 spin columns, wash buffer, elution reagents, collection tubes

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$771.00 
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ProteoMiner™ Protein Enrichment Large-Capacity Kit

1633007
10 preps, low-abundance protein enrichment kit for 50 mg of total protein, includes 10 spin columns, wash buffer, elution reagents, collection tubes

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$1,058.00 
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ReadyPrep™ 2-D Cleanup Kit for IEF, 50 preps

1632130
50 preps, includes 2 precipitation agents, 2 wash reagents, 1 wash reagent additive; for 50 x 100 µl protein samples each containing up to 500 µg protein

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$366.00 
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ReadyPrep™ 2-D Starter Kit

1632105
2-D gel electrophoresis kit, includes protein sample and buffers for IPG strip rehydration, focusing, and transfer to second dimension (IPG strips not included)

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$304.00 
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Gels

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18% Criterion™ TGX™ Precast Midi Protein Gel, 11 cm IPG/prep+1 well, 550 µl

5671071
Pkg of 1, 18% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$15.00 
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4–15% Criterion™ TGX™ Precast Midi Protein Gel, 11 cm IPG/prep+1 well, 550 µl

5671081
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$15.00 
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4–20% Criterion™ TGX™ Precast Midi Protein Gel, 11 cm IPG/prep+1 well, 550 µl

5671091
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$15.00 
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8–16% Criterion™ TGX™ Precast Midi Protein Gel, 11 cm IPG/prep+1 well, 550 µl

5671101
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$15.00 
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10–20% Criterion™ TGX™ Precast Midi Protein Gel, 11 cm IPG/prep+1 well, 550 µl

5671111
Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • AnykD™ Criterion™ TGX™ Precast Midi Protein Gel, 11 cm IPG/prep+1 well, 550 µl (5671121)
AnykD™ Criterion™ TGX™ Precast Midi Protein Gel, 11 cm IPG/prep+1 well, 550 µl

5671121
Pkg of 1, AnykD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$15.00 
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IPG Strips

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ReadyStrip™ IPG Strips

1632014
Pkg of 12, 11 cm, pH 3–10, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems

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$146.00 
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ReadyStrip™ IPG Strips

1632015
Pkg of 12, 11 cm, pH 4–7, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems

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$141.00 
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ReadyStrip™ IPG Strips

1632016
Pkg of 12, 11 cm, pH 3–10 nonlinear, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems

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$141.00 
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ReadyStrip™ IPG Strips

1632017
Pkg of 12, 11 cm, pH 3–6, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems

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$141.00 
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ReadyStrip™ IPG Strips

1632018
Pkg of 12, 11 cm, pH 5–8, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems

List Price: 
 
$141.00 
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ReadyStrip™ IPG Strips

1632019
Pkg of 12, 11 cm, pH 7–10, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems

List Price: 
 
$141.00 
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(Discontinued) ReadyStrip™ IPG Strips

1632024
Pkg of 12, 11 cm, pH 3.9–5.1, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems

This product is no longer available. Please contact technical support for more information on replacement product.
(Discontinued) ReadyStrip™ IPG Strips

1632026
Pkg of 12, 11 cm, pH 5.5–6.7, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems

This product is no longer available. Please contact technical support for more information on replacement product.

The following replacement is recommended:

  • ReadyStrip™ IPG Strips (1632018)
(Discontinued) ReadyStrip™ IPG Strips

1632027
Pkg of 12, 11 cm, pH 6.3–8.3, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems

This product is no longer available. Please contact technical support for more information on replacement product.

Documents

Number Description Options
6478 2-D Electrophoresis Workflow Brochure, Rev B Click to download [ Add to Cart (Free) ]
6493 Bio-Rad’s Host Cell Protein (HCP) Workflow Quick Guide Click to download [ Add to Cart (Free) ]
2651 2-D Electrophoresis Workflow How-To Guide, Rev F Click to download
5635 ProteoMiner Protein Enrichment Technology Bulletin, Rev B Click to download
10010636 Instruction Manual, ProteoMiner Protein Enrichment Kits, Rev D Click to download
4110143 Instruction Manual, ReadyPrep 2-D Cleanup Kit, Rev A Click to download
4110009 Instruction Manual, ReadyPrep 2-D Starter Kit, Rev A Click to download
2642 ReadyPrep 2-D Starter Kit Product Information Sheet Click to download
6097 PROTEAN i12 IEF System Brochure, Ver B Click to download [ Add to Cart (Free) ]
6138 PROTEAN i12 IEF System: Independent Voltage and Current Control Enables Optimization of First-Dimension IEF Conditions, Rev A Click to download
4006166 Instruction Manual, ReadyStrip IPG Strip Click to download
2442 ReadyStrip IPG Strip Product Information Sheet, Rev F Click to download
4006197 Instruction Manual, Criterion Dodeca Cell, Rev A Click to download
6040 Electrophoresis Guide, Interactive PDF, Rev C Click to download
4110001 Instruction Manual, Criterion™ Gel Application Guide, Ver H Click to download
5991 Oriole Fluorescent Gel Stain: A Novel Fluorescent Stain for a Simpler 2-D PAGE to MALDI Workflow, Rev A Click to download
5900 Oriole Fluorescent Gel Stain PIS, Rev A Click to download
6427 Bio-Rad Stain-Free Western Blotting Click to download [ Add to Cart (Free) ]
3121 PDQuest 2-D Analysis Software Brochure, Rev C Click to download
3194 EXQuest Spot Cutter Brochure, Rev A Click to download
10001531 Instruction Manual, EXQuest™ Spot Cutter, Rev A Click to download
6371 Electrophoresis Power Supplies Brochure, Rev C Click to download [ Add to Cart (Free) ]
6393 Reliable, Streamlined 2-D Western Blot Workflow for Evaluation of Antibodies Developed for Detection of Host Cell Proteins, Rev A Click to download [ Add to Cart (Free) ]
6405 Effectiveness of 2-D Electrophoresis over 1-D Electrophoresis Followed by Western Blotting for Evaluating Strategies Used to Generate Host Cell Protein Antibodies, Rev A Click to download

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