QX200 AutoDG Droplet Digital PCR System

Image
qx200-autodg-droplet-digital-pcr-system-detail.jpg

Overview

The AutoDG Instrument simplifies the Droplet Digital PCR workflow, making digital PCR both scalable and practical. Prepare up to 96 samples at a time with minimal hands-on time and eliminate user-to-user variability.

Read full description Ordering Information

Description

Description

 

The QX200 AutoDG Droplet Digital PCR System provides absolute quantification of target DNA or RNA molecules for EvaGreen and probe-based Droplet Digital PCR (ddPCR) applications. The Automated Droplet Generator simplifies the ddPCR workflow, making digital PCR both scalable and practical.

The AutoDG Instrument eliminates user-to-user variability, allowing you to fully exploit the precision of ddPCR technology by providing more consistent droplet counts across users and plates. The AutoDG Instrument also minimizes hands-on time — it takes less than 5 minutes to load the instrument, and no supervision or user activity is required during droplet generation.

Features and Benefits of the QX200 AutoDG Droplet Digital PCR System

Automated Droplet Generator — simplifies the ddPCR workflow and offers flexibility:

  • Generates droplets for 96 ddPCR reactions in under 45 minutes
  • Compatible with both the QX100 and QX200 Droplet Digital PCR Systems
  • A single AutoDG Instrument can supply 4–5 Droplet Readers continuously
  • Guides setup and loading with large, color touch-screen interface and LED lighting
  • User-definable plate layout with increments of 8 wells provides run-to-run flexibility
  • HEPA-filtered enclosure reduces the possibility of contamination and allows the system to be placed in any lab environment

Droplet Digital PCR Technology — the most precise and sensitive digital PCR solution for a wide variety of applications, providing:

  • Flexible digital PCR chemistry — optimized for TaqMan Hydrolysis Probe and EvaGreen Assays
  • Nanoliter droplet partitioning — reduces bias from amplification efficiency differences and PCR inhibitors
  • Convenient assay design — standard curves are not required

Applications of the QX200 Droplet Digital PCR System

  • Cancer biomarker studies and copy number variation — measure varying degrees of cancer mutations, detect rare DNA target copies, and resolve copy number variation states with superior sensitivity and resolution.
  • Pathogen detection — employ the extreme precision of the QX200 System to quantify small fold changes in target DNA or RNA molecules in pathogen detection and monitoring
  • Next generation sequencing — perform absolute quantification of an NGS library without the use of a standard curve
  • Gene expression analysis — achieve reliable and reproducible measurements of small fold changes for low-abundance mRNA and miRNA
  • Environmental monitoring — test a wide variety of environmental samples such as soil and water
  • Food testing — perform routine evaluation of genetically modified organisms (GMO) using validated ddPCR methods

Required Instruments

The QX200 AutoDG Droplet Digital PCR System requires the use of the C1000 Touch Thermal Cycler with 96–Deep Well Reaction Module and the PX1 PCR Plate Sealer.

Required Consumables

ddPCR Reagents for EvaGreen or TaqMan Hydrolysis Probe-Based Applications

More Information

Download the Droplet Digital PCR Applications Guide for in-depth information about setting up experiments for applications including:

  • Droplet Digital PCR applications guide
    • Copy number variation analysis
    • Rare mutation and sequence detection
    • Gene expression
    • Next-generation sequencing library analysis

 

View Performance Data for the QX200 Droplet Digital PCR System.

 

Watch our Success Stories Videos to learn more about the applications of the Droplet Digital PCR System in diverse research fields.

Browse our Droplet Digital PCR Reference Library with links to scientific journal articles, conference posters, BioRadiations articles, and Bio-Rad technical reports.

Visit our Applications & Technologies pages to learn more about Droplet Digital PCR Technology and the digital PCR workflow.

Specifications

Specifications

Starting sample size, µl
20
Automated Droplet Generator capacity
1–96 samples/run
Droplets per 20 µl sample
20,000
QX200™ Droplet Reader capacity
1–96 samples
Sample illumination
Light-emitting diodes
Sample detection
Multi-pixel photon counter
Detection channels
FAM (EvaGreen), HEX (VIC)
Linear dynamic range
5 orders of magnitude
Precision
±10%
Droplets per 96-well plate, million
~1.5
Automated Droplet Generator dimensions (W x D x H)
Open: 66 x 56 x 89 cm (26 x 22 x 35 in)
Closed: 66 x 56 x 66 cm (26 x 22 x 26 in)
QX200 Droplet Reader dimensions (W x D x H)
66 x 52 x 29 cm (26 x 20 x 11 in)

Ordering

Ordering

items

Use the filters below to refine results!

Image
qx200-auto-dg-droplet-digital-pcr-system-186-4100-thumb.jpg
1864100

AutoDG Droplet Digital PCR System for EvaGreen or probe-based digital PCR applications, includes Automated Droplet Generator, QX200 Droplet Reader, computer, and QX Manager Software, Standard Edition.

Image
automated-droplet-generator-186-4101-thumb.jpg
1864101
AutoDG Instrument for automated droplet generation of up to 96 samples, for use with the QX200/QX100; Droplet Digital PCR Systems
Image
12012172_th.jpg
12012172
QX200 System Control and Analysis Software, with traceablity for secure environments

Accessories

Accessories

Image
PX1-PCR-Plate-Sealer-SKU-181-4000-thumb.jpg
(Discontinued) PX1 PCR Plate Sealer
1814000
PCR plate sealer, includes heat sealing instrument, plate support block that holds 96-well and 384-well plates, sealing frame, power cord
Image
185-1196_tn.jpg
1851197

Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, 96–deep well reaction module, USB flash drive

Kits & Reagents

Kits & Reagents

Image
12005909_thumb.jpg
12005909

1.2 ml (2 x 0.6 ml), 4x supermix, for use in sample preparation for droplet generation in the QX200 Droplet Digital PCR Systems

Image
12005910_thumb.jpg
12005910

3 ml (5 x 0.6 ml), 4x supermix, for use in sample preparation for droplet generation in the QX200 Droplet Digital PCR Systems

Image
12005911_thumb.jpg
12005911
12.5 ml (5 x 2.5 ml), 4x supermix, for use in sample preparation for droplet generation in the QX200 Droplet Digital PCR Systems
Image
qx100-droplet-pcr-supermix-2ml-186-3023-thumb.jpg
1863023

2 ml (2 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX200/QX100 Droplet Digital PCR Systems

Image
qx100-droplet-pcr-supermix-5ml-186-3024-thumb.jpg
1863024

5 ml (5 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX200/QX100 Droplet Digital PCR Systems

Image
qx100-droplet-pcr-supermix-25ml-186-3025-thumb.jpg
1863025
25 ml (5 x 5 ml), 2x supermix, for use in sample preparation for droplet generation in the QX200/QX100 Droplet Digital PCR Systems
Image
qx100-ddpcr-supermix-probes-2ml-186-3026-thumb.jpg
1863026
2 ml (2 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX200™/QX100™ Droplet Digital™ PCR Systems
Image
qx100-ddpcr-supermix-probes-5ml-186-3010-tn.jpg
1863010
5 ml (5 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX200™/QX100™ Droplet Digital™ PCR Systems
Image
qx100-ddpcr-supermix-probes-25ml-186-3027-tn.jpg
1863027
25 ml (5 x 5 ml), 2x supermix, for use in sample preparation for droplet generation in the QX200™/QX100™ Droplet Digital™ PCR Systems
Image
qx100-ddpcr-supermix-probes-50ml-186-3028-thumb.jpg
1863028
50 ml (10 x 5 ml), 2x supermix, for use in sample preparation for droplet generation in the QX200™/QX100™ Droplet Digital™ PCR Systems
Image
qx200-ddpcr-evagreen-supermix-186-4033-thumb.jpg
1864033
200 x 20 µl reactions, 2 ml (2 x 1 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX200™ Droplet Digital™ PCR System
Image
qx200-evagreen-ddpcr-supermix-186-4034-thumb.jpg
1864034

500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX200™ Droplet Digital™ PCR System

Image
qx200-evagreen-ddpcr-supermix-186-4035-thumb.jpg
1864035
2,500 x 20 µl reactions, 25 ml (5 x 5 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX200™ Droplet Digital™ PCR System
Image
qx200-evagreen-ddpcr-supermix-186-4036-thumb.jpg
1864036
5,000 x 20 µl reactions, 50 ml (10 x 5 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX200™ Droplet Digital™ PCR System
Image
one-step-rt-ddpcr-advanced-kit-for-probes-1864021-thumb.jpg
1864021

200 x 20 µl reaction kit, for absolute quantification of target RNA in the QX200™AutoDG™/QX200™/QX100™ Droplet Digital™ PCR Systems

Image
one-step-rt-ddpcr-advanced-kit-for-probes-1864022-thumb.jpg
1864022

500 x 20 µl reaction kit, for absolute quantification of target RNA in the QX200™AutoDG™/QX200™/QX100™ Droplet Digital™ PCR Systems

Image
ddpcr-library-quantification-kit-for-ilumina-truseq-186-3040-thumb.jpg
1863040

200 x 20 µl reactions, includes ddPCR Library Quantification Assay and ddPCR Supermix for Probes (No dUTP), for quantification of Illumina TruSeq libraries using the QX200™/QX100™ ddPCR™ Systems

Consumables

Consumables

Image
droplet-digital-pcr-plate-stack-12001925-thumb.jpg
12001925

Pkg of 25, clear well/clear shell semi-skirted plates, for use with QX200™/QX100™ or QX200™ AutoDG™ Droplet Digital™ PCR Systems

Image
dg32-automated-droplet-generator-cartridges-186-4008-thumb.jpg
1864108
Pkg of 30, cartridges for use with the Automated Droplet Generator in the QX200 AutoDG Droplet Digital PCR System
Image
dg32-automated-droplet-generator-cartridges-186-4009-thumb.jpg
1864109
Pkg of 60, cartridges for use with the Automated Droplet Generator in the QX200™ AutoDG™ Droplet Digital™ PCR System
Image
automated-droplet-generation-oil-for-probes-186-4110-thumb.jpg
1864110
140 ml, oil for use with the Automated Droplet Generator for probe-based detection with the QX200 AutoDG Droplet Digital PCR System
Image
automated-droplet-generation-oil-for-evagreen-186-4112-thumb.jpg
1864112
140 ml, oil for use with the Automated Droplet Generator for EvaGreen-based detection with the QX200 AutoDG Droplet Digital PCR System
Image
pipet-tips-for-the-autodg-system-186-4120-thumb.jpg
1864120
Pkg of 20, boxes of 96 each, pipet tips for use with the Automated Droplet Generator in the QX200™ AutoDG™ Droplet Digital™ PCR System
Image
pipet-tips-for-the-autodg-system-186-4121-thumb.jpg
1864121
Pkg of 40, boxes of 96 each, pipet tips for use with the Automated Droplet Generator in the QX200™ AutoDG™ Droplet Digital™ PCR System
Image
pipet-waste-bins-for-the-auto-dg-system-186-4125-thumb.jpg
1864125
Pkg of 10, pipet tip waste bins for use with the Automated Droplet Generator in the QX200™ AutoDG™ Droplet Digital™ PCR System
Image
pierceable-foil-heat-seal-181-4040-thumb.jpg
1814040
Pkg of 100, foil seals for PCR and QX200/QX100 Droplet Digital PCR System applications, for use with PX1 PCR Plate Sealer

Documents

Documents

Number Description Options
6718
Guidelines for Phasing Variants Using Bio-Rad's Droplet Digital PCR System Application Note
6561
QX200 AutoDG Droplet Digital PCR System Flier
[ Add to Cart (Free) ]
10043138
Instruction Manual, Automated Droplet Generator for Droplet Digital™ PCR Systems
10000107223
QX Manager Software Standard Edition, User Guide
10000107224
QX Manager Software Regulatory Edition, User Guide
10000118144
QX Developer Software, User Guide
10028376
QX200™ ddPCR™ EvaGreen Supermix Product Insert
10048259
ddPCR Supermix for Residual DNA Quantification Product Insert
6782
ddPCR™ Multiplex Mutation Screening Kits Flier, Ver D
[ Add to Cart (Free) ]
6721
ddPCR™ Supermix for Residual DNA Quantification Flier, Rev A
6712
Ultra-Sensitive Quantification of Genome Editing Events Using Droplet Digital™ PCR Application Note, Ver B
7204
Expanded Droplet Digital PCR Multiplexing Capability Using Two Different Strategies, Ver A
7271
Current Challenges and Future Impact of Cellular and Genetic Immunotherapies for Human Health