iQ SYBR Green Supermix

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iQ SYBR Green Supermix

Overview

Quantitation and Precision

iQ™ SYBR® Green supermix generates precise, quantitative results. A 5-fold dilution series (50 ng–80 pg) of human genomic DNA was amplified using the supermix and primers and a probe specific to the IL-1β gene. Triplicate reactions at each concentration were amplified along with no-template controls on the iCycler iQ® real-time system. r = 0.999, slope = –3.380, efficiency = 97.6%, RFU, relative fluorescence units.

Broad Dynamic Range

iQ™ SYBR® Green Supermix and the iScript™ cDNA synthesis kit show high specificity over a broad dynamic range of cDNA.

Serial dilutions (1 µg–1 pg) of HeLa total RNA were reverse transcribed, and the resulting cDNA was amplified using primers specific to the β-actin gene. Triplicate reactions at each concentration were amplified along with no-template controls on the iCycler iQ® Real-Time System. The consistent spacing of the curves reflects accurate reverse transcription and amplification. r = 0.997, slope = -3.615, efficiency = 89.1%, RFU, relative fluorescence units.



Specificity With Plasmid DNA

iQ™ SYBR® Green supermix shows high specificity with plasmid templates. Tenfold serial dilutions (108 to 102 copies) of plasmid DNA were amplified using primers specific to the β-actin gene. Triplicate reactions at each concentration were amplified along with no-template controls on the iCycler iQ® real-time system. r = 1.000, slope = -3.492, efficiency = 93.4%, RFU, relative fluorescence units.

Analyze low-, medium-, and high-abundance target genes with good sensitivity, specificity, and efficiency using dye-based qPCR.
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Description

iQ SYBR Green Supermix is formulated to achieve optimal results for a wide array of real-time PCR applications with the convenience of SYBR Green I detection chemistry. This preblended 2x solution contains an optimized concentration of SYBR Green I dye for sensitive and accurate detection, hot-start iTaq DNA Polymerase, a proprietary buffer and stabilizers specifically optimized for qPCR using SYBR Green I, and qPCR-qualified dNTPs. This supermix also contains fluorescein, which is used as a passive reference dye for well-factor collection on certain real-time PCR detection systems. Use of iQ SYBR Green Supermix minimizes preparation time, produces reliable results for real-time PCR assays, and provides precise linear detection over six orders of magnitude.

Key Features and Benefits

  • Increased throughput — requires minimal preparation since only template and primers are needed
  • High sensitivity and accuracy — the antibody-mediated hot-start iTaq DNA Polymerase minimizes amplification of nonspecific PCR products and allows sensitive and accurate detection of as few as 10 copies of template
  • High specificity over a broad dynamic range — the combination of the iQ SYBR Green Supermix with the iScript cDNA Synthesis Kit delivers effective results over a broad dynamic range of cDNA

Applications and Uses

  • PCR and real-time PCR analysis
  • Gene expression analysis
  • Genotyping studies
  • Genomics-related applications

Related Products

Select the best real-time PCR supermix for your application:

  • Bio-Rad’s Universal Supermixes can be used in all real-time PCR systems — both ROX-dependent and ROX-independent instruments
  • SsoAdvanced Universal Supermixes feature our patented Sso7d fusion polymerase
Key Features of Bio-Rad Real-Time PCR Supermixes iQ
Supermixes
SsoAdvanced Universal Supermixes
Sso7d fusion protein technology No Yes
Sensitive detection of low-level targets
Efficiency with difficult target sequences
Specificity
Tolerance to PCR inhibitors
Broad range of reaction conditions
Fast cycling
Instrument compatibility Select instruments Any instrument

Related Categories

* U.S. patents 6,627,424; 7,541,170; and 7,560,260.


Specifications

Storage at –20°C Guaranteed for 12 months at –20°C in a constant temperature freezer (multiple freeze thaws not recommended)
Storage at +4°C Guaranteed for 6 months at 4°C (protected from light)

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iQ™ SYBR® Green Supermix, 100 x 50 µl rxns, 2.5 ml (2 x 1.25 ml)

1708880
2.5 ml (2 x 1.25 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, for 100 x 50 µl reactions

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$246.00 
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iQ™ SYBR® Green Supermix, 500 x 50 µl rxns, 12.5 ml (10 x 1.25 ml)

1708882
12.5 ml (10 x 1.25 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, for 500 x 50 µl reactions

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$1,060.00 
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iQ™ SYBR® Green Supermix, 1,000 x 50 µl rxns, 25 ml (20 x 1.25 ml)

1708884
25 ml (20 x 1.25 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, for 1,000 x 50 µl reactions

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$2,044.00 
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iQ™ SYBR® Green Supermix, 2,000 x 50 µl rxns, 50 ml (40 x 1.25 ml)

1708884BUN
50 ml (40 x 1.25 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, for 2,000 x 50 µl reactions

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$4,010.00 
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iQ™ SYBR® Green Supermix, 2,000 x 50 µl rxns, 50 ml (1 x 50 ml)

1708885
50 ml (1 x 50 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, for 2,000 x 50 µl reactions

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$4,010.00 
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iQ™ SYBR® Green Supermix, 1,000 x 50 µl rxns, 25 ml (5 x 5 ml)

1708886
25 ml (5 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, for 1,000 x 50 μl reactions

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$1,896.00 
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iQ™ SYBR® Green Supermix, 2,000 x 50 µl rxns, 50 ml (10 x 5 ml)

1708887
50 ml (10 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, for 2,000 x 50 µl reactions

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$3,520.00 
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Documents

Number Description Options
4106212 Instruction Manual, iQ SYBR Green Supermix, 100 x 50 µl, Rev D Click to download
10016680 iQ™ SYBR® Green Supermix User Guide, Rev A Click to download
2764 iQ Supermixes for qPCR Flier, Rev E Click to download
6252 Reagent Comparison Guide for Real-Time PCR Brochure, Rev C Click to download [ Add to Cart (Free) ]
7178 Amplification: Real-Time PCR Reagents and Kits Brochure Click to download
6090 Amplification Reagents and Plastics Brochure Click to download [ Add to Cart (Free) ]
6763 Gene Expression Real-Time PCR Workflow Flier Click to download

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