iQ SYBR Green Supermix

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Overview

Analyze low-, medium-, and high-abundance target genes with good sensitivity, specificity, and efficiency using dye-based qPCR.

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Description

Description

iQ SYBR Green Supermix is formulated to achieve optimal results for a wide array of real-time PCR applications with the convenience of SYBR Green I detection chemistry. This preblended 2x solution contains an optimized concentration of SYBR Green I dye for sensitive and accurate detection, hot-start iTaq DNA Polymerase, a proprietary buffer and stabilizers specifically optimized for qPCR using SYBR Green I, and qPCR-qualified dNTPs. This supermix also contains fluorescein, which is used as a passive reference dye for well-factor collection on certain real-time PCR detection systems. Use of iQ SYBR Green Supermix minimizes preparation time, produces reliable results for real-time PCR assays, and provides precise linear detection over six orders of magnitude.

Key Features and Benefits

  • Increased throughput — requires minimal preparation since only template and primers are needed
  • High sensitivity and accuracy — the antibody-mediated hot-start iTaq DNA Polymerase minimizes amplification of nonspecific PCR products and allows sensitive and accurate detection of as few as 10 copies of template
  • High specificity over a broad dynamic range — the combination of the iQ SYBR Green Supermix with the iScript cDNA Synthesis Kit delivers effective results over a broad dynamic range of cDNA

Applications and Uses

  • PCR and real-time PCR analysis
  • Gene expression analysis
  • Genotyping studies
  • Genomics-related applications

Related Products

Select the best real-time PCR supermix for your application:

  • Bio-Rad’s Universal Supermixes can be used in all real-time PCR systems — both ROX-dependent and ROX-independent instruments
  • SsoAdvanced Universal Supermixes feature our patented Sso7d fusion polymerase
Key Features of Bio-Rad Real-Time PCR Supermixes iQ
Supermixes
SsoAdvanced Universal Supermixes
Sso7d fusion protein technology No Yes
Sensitive detection of low-level targets
Efficiency with difficult target sequences
Specificity
Tolerance to PCR inhibitors
Broad range of reaction conditions
Fast cycling
Instrument compatibility Select instruments Any instrument

 

Related Categories

* U.S. patent 7,560,260.

Specifications

Specifications

Storage at –20°C
Guaranteed for 12 months at –20°C in a constant temperature freezer (multiple freeze thaws not recommended)
Storage at +4°C
Guaranteed for 6 months at 4°C (protected from light)

Ordering

Ordering

items

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170-8880_tn.jpg
1708880
2.5 ml (2 x 1.25 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, for 100 x 50 µl reactions
170-8882_tn.jpg
1708882
12.5 ml (10 x 1.25 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, for 500 x 50 µl reactions
170-8884_tn.jpg
1708884
25 ml (20 x 1.25 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, for 1,000 x 50 µl reactions
170-8884BUN_tn.jpg
1708884BUN
50 ml (40 x 1.25 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, for 2,000 x 50 µl reactions
170-8885_tn.jpg
1708885
50 ml (1 x 50 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, for 2,000 x 50 µl reactions
170-8886_tn.jpg
1708886
25 ml (5 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, for 1,000 x 50 μl reactions
170-8887_tn.jpg
1708887
50 ml (10 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, for 2,000 x 50 µl reactions

Documents

Documents

Number Description Options
4106212
Instruction Manual, iQ SYBR Green Supermix, 100 x 50 µl, Rev D
10016680
iQ™ SYBR® Green Supermix User Guide, Rev A
2764
iQ Supermixes for qPCR Flier, Rev E
6252
Reagent Comparison Guide for Real-Time PCR Brochure, Rev C
[ Add to Cart (Free) ]
7178
Amplification: Real-Time PCR Reagents and Kits Brochure
6090
Amplification Reagents and Plastics Brochure
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6763
Gene Expression Real-Time PCR Workflow Flier
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