iQ SYBR Green Supermix

Analyze low-, medium-, and high-abundance target genes with good sensitivity, specificity, and efficiency using dye-based qPCR.

Ordering Information

Overview

iQ SYBR Green Supermix is formulated to achieve optimal results for a wide array of real-time PCR applications with the convenience of SYBR Green I detection chemistry. This preblended 2x solution contains an optimized concentration of SYBR Green I dye for sensitive and accurate detection, hot-start iTaq DNA Polymerase, a proprietary buffer and stabilizers specifically optimized for qPCR using SYBR Green I, and qPCR-qualified dNTPs. This supermix also contains fluorescein, which is used as a passive reference dye for well-factor collection on certain real-time PCR detection systems. Use of iQ SYBR Green Supermix minimizes preparation time, produces reliable results for real-time PCR assays, and provides precise linear detection over six orders of magnitude.

Key Features and Benefits

Increased throughput — requires minimal preparation since only template and primers are needed
High sensitivity and accuracy — the antibody-mediated hot-start iTaq DNA Polymerase minimizes amplification of nonspecific PCR products and allows sensitive and accurate detection of as few as 10 copies of template
High specificity over a broad dynamic range — the combination of the iQ SYBR Green Supermix with the iScript cDNA Synthesis Kit delivers effective results over a broad dynamic range of cDNA

Applications and Uses

PCR and real-time PCR analysis
Gene expression analysis
Genotyping studies
Genomics-related applications

Related Products

Select the best real-time PCR supermix for your application:

Bio-Rad’s Universal Supermixes can be used in all real-time PCR systems — both ROX-dependent and ROX-independent instruments
SsoAdvanced Universal Supermixes feature our patented Sso7d fusion polymerase

Key Features of Bio-Rad Real-Time PCR Supermixes	iQ Supermixes	SsoAdvanced Universal Supermixes
Sso7d fusion protein technology	No	Yes
Sensitive detection of low-level targets
Efficiency with difficult target sequences
Specificity
Tolerance to PCR inhibitors
Broad range of reaction conditions
Fast cycling
Instrument compatibility	Select instruments	Any instrument

Related Categories

* U.S. patent 7,560,260.

Related Technologies

Specifications

Storage at –20°C

Guaranteed for 12 months at –20°C in a constant temperature freezer (multiple freeze thaws not recommended)

Storage at +4°C

Guaranteed for 6 months at 4°C (protected from light)

Ordering

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Use the filters below to refine results!

(Discontinued) iQ™ SYBR^® Green Supermix, 100 x 50 µl rxns, 2.5 ml (2 x 1.25 ml)

1708880

2.5 ml (2 x 1.25 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA Polymerase, MgCl₂, SYBR^® Green I, enhancers, stabilizers, fluorescein, for 100 x 50 µl reactions

(Discontinued) iQ™ SYBR^® Green Supermix, 500 x 50 µl rxns, 12.5 ml (10 x 1.25 ml)

1708882

12.5 ml (10 x 1.25 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA Polymerase, MgCl₂, SYBR^® Green I, enhancers, stabilizers, fluorescein, for 500 x 50 µl reactions

(Discontinued) iQ™ SYBR^® Green Supermix, 1,000 x 50 µl rxns, 25 ml (20 x 1.25 ml)

1708884

25 ml (20 x 1.25 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA Polymerase, MgCl₂, SYBR^® Green I, enhancers, stabilizers, fluorescein, for 1,000 x 50 µl reactions

(Discontinued) iQ™ SYBR^® Green Supermix, 2,000 x 50 µl rxns, 50 ml (40 x 1.25 ml)

1708884BUN

50 ml (40 x 1.25 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA Polymerase, MgCl₂, SYBR^® Green I, enhancers, stabilizers, fluorescein, for 2,000 x 50 µl reactions

(Discontinued) iQ™ SYBR^® Green Supermix, 2,000 x 50 µl rxns, 50 ml (1 x 50 ml)

1708885

50 ml (1 x 50 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA Polymerase, MgCl₂, SYBR^® Green I, enhancers, stabilizers, fluorescein, for 2,000 x 50 µl reactions

(Discontinued) iQ™ SYBR^® Green Supermix, 1,000 x 50 µl rxns, 25 ml (5 x 5 ml)

1708886

25 ml (5 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA Polymerase, MgCl₂, SYBR^® Green I, enhancers, stabilizers, fluorescein, for 1,000 x 50 μl reactions

(Discontinued) iQ™ SYBR^® Green Supermix, 2,000 x 50 µl rxns, 50 ml (10 x 5 ml)

1708887

50 ml (10 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA Polymerase, MgCl₂, SYBR^® Green I, enhancers, stabilizers, fluorescein, for 2,000 x 50 µl reactions

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