iQ Multiplex Powermix

iQ Multiplex Powermix simplifies real-time detection of multiple targets in a single tube. Although design software, such as Beacon Designer software, has made it easier to design effective primers and probes, finding a set of reaction conditions that amplifies all targets with equal efficiency in both singleplex and multiplex reactions can still be a challenge. iQ Multiplex Powermix makes multiplex real-time PCR easier by removing the need to optimize buffer, enzyme, or primer concentrations. This mix ensures:

• Reliable real-time multiplex detection of up to 5 targets
• Linearity over 6 orders of magnitude of input cDNA and 4 orders of magnitude of input genomic DNA
• Detection of up to 4 targets when one differs in expression up to 10⁶-fold relative to the others

With this reliable mix, you can increase throughput and control costs by running multiple assays in a single reaction, maximizing the amount of data collected from limited amounts of sample. iQ multiplex powermix is formulated for analysis using cDNA, genomic DNA, and plasmids, and can be used for a wide variety of applications, including:

• Gene expression analysis
• SNP genotyping/SNP analysis
• GMO detection
• Viral load detection

dUTP Discontinuation Notice

Bio-Rad supermixes containing dUTP have been discontinued and replaced with mixes containing dTTP. The reasons for eliminating dUTP from these supermixes are:

• The high specificity of PCR and quantitative PCR (qPCR) and the use of disposable consumables and filter tips enable the elimination of dUTP and UNG from typical qPCR processes
• All researchers in a particular facility must use dUTP and UNG for them to be effective; if any researcher is not using them, they will not be effective controls
• dTTP mixes offer better efficiency, especially for GC-rich templates
• dUTP and UNG add significant cost to performing qPCR, since UNG must be purchased separately
• dTTP-containing mixes maintain superb quality and high performance in qPCR