SsoFast EvaGreen Supermix with Low ROX

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Overview

This version of SsoFast qPCR supermix with EvaGreen includes ROX and is compatible with ROX-dependent real-time instruments, such as Applied Biosystems 7500 and ViiA 7.
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Description

Description

NEW PRODUCT
SsoAdvanced Universal Supermixes

Affordable, superior performance alternative to SsoFast Supermixes

  • Guaranteed performance under all conditions
  • Use on any real-time PCR instrument without adjusting ROX concentration
SsoAdvanced Universal Supermixes

SsoFast EvaGreen Supermix with Low ROX is a ROX-blended version of our high-performing SsoFast EvaGreen Supermix, which employs the patented Sso7d fusion protein technology* for use in real-time PCR applications. This novel, engineered polymerase, combined with optimized buffer and EvaGreen dye, produces robust qPCR results in less time with increased speed, reliability, and sensitivity under fast thermal cycling conditions.

Key Features and Benefits

  • Fast qPCR results — Sso7d fusion polymerase's stringent hot-start and rapid polymerization kinetics provide consistent qPCR results under fast cycling conditions (approximately 40 min)
  • Preblended with ROX — streamlines use with real-time PCR systems that require ROX for fast runs
  • Minimal PCR inhibition — highly tolerant polymerase and EvaGreen dye exhibit low PCR inhibition and provide better performance with fast cycling protocols
  • Single-copy detection — higher concentrations generate larger fluorescent signals to provide increased sensitivity
  • Broad dynamic range — yields linear results over 6 orders of magnitude on real-time PCR systems

Applications and Uses

  • Dye-based qPCR detection
  • PCR and real-time PCR analysis
  • ROX-dependent real-time PCR detection
  • Gene expression analysis
  • Genotyping studies
  • Genomics-related applications

Related Products

Select the best real-tme PCR supermix for your application:

  • Bio-Rad’s Universal Supermixes can be used in all real-time PCR systems — both ROX-dependent and ROX-independent instruments
  • SsoAdvanced Universal Supermixes feature our patented Sso7d fusion polymerase

Key Features of Bio-Rad Real-Time PCR Supermixes

SsoFast
Supermixes

SsoAdvanced Universal Supermixes

Sso7d fusion protein technology

Yes

Yes

Sensitive detection of low-level targets

Efficiency with difficult target sequences

Specificity

Tolerance to PCR inhibitors

Broad range of reaction conditions

Fast cycling

Instrument compatibility

Select instruments

Any instrument


Related Categories

* U.S. patents 6,627,424; 7,541,170; and 7,560,260.

Specifications

Specifications

Product expiration (after shipping date)
12 months
Storage at 4°C (after thawing)
Up to 6 months
Customer guarantee (minimum usage time) at –20°C
12 months

Ordering

Ordering

items

Use the filters below to refine results!

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1725211
5 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, ROX passive reference dye, stabilizers, for 500 x 20 µl reactions
172-5212_tn.jpg
1725212
10 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, ROX passive reference dye, stabilizers, for 1,000 x 20 µl reactions
172-5213_tn.jpg
1725213
20 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, ROX passive reference dye, stabilizers, for 2,000 x 20 µl reactions

Documents

Documents

Number Description Options
10016588
Instruction Manual, SsoFast™ EvaGreen® Supermix With Low ROX, Rev. B
6252
Reagent Comparison Guide for Real-Time PCR Brochure, Rev C
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7178
Amplification: Real-Time PCR Reagents and Kits Brochure
6090
Amplification Reagents and Plastics Brochure
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6763
Gene Expression Real-Time PCR Workflow Flier
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