SsoFast EvaGreen Supermix with Low ROX

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SsoFast EvaGreen Supermix with Low ROX

Overview

Sso7d Fusion Protein Technology
Bio-Rad’s patented* Sso7d fusion protein technology was first employed in the iProof™ high fidelity DNA polymerase product line. The unique Sso7d fusion polymerase in the SsoFast™ supermixes enables fast cycling without affecting PCR sensitivity, efficiency, and reproducibility. The dsDNA binding protein, Sso7d, stabilizes the polymerase-template complex, increases processivity, and provides greater speed and reduced reaction times compared to conventional DNA polymerases. Furthermore, this polymerase is significantly more resistant to PCR inhibitors, making the SsoFast  supermixes ideal choices for applications such as direct qPCR, without the need for sample preparation.
 
Fusing the double-stranded DNA binding protein Sso7d to DNA polymerases provides a powerful sliding grip on the replicated DNA.
 
* U.S. patents 6,627,424; 7,541,170; and 7,560,260.
Broad Dynamic Range Under Fast Cycling Conditions

The fusion polymerase in SsoFast™ EvaGreen® Supermix with Low ROX demonstrates a broad dynamic range under fast cycling conditions using the ABI 7500 fast Real-Time PCR System.

SsoFast EvaGreen Supermix with Low ROX generates consistent qPCR results on the ABI 7500 fast Real-Time PCR System. Tenfold serial dilutions from 10 ng to 100 fg of cDNA were used in each 20 µl reaction.

cDNA from human spleen was used to detect α-tubulin (α-tubulin efficiency = 105.8%, r = 0.996; total qPCR run time = 39 min, not including melt curve).



cDNA from human liver was used to detect TIP (TIP efficiency = 92.7%, r = 0.992; total qPCR run time = 42 min, not including melt curve).



Broad Dynamic Range Over Six Orders of Magnitude

SsoFast™ EvaGreen® supermix with low ROX generates linear results over six orders of magnitide on the ABI 7500 fast real-time PCR system. Tenfold serial dilutions of 100 ng to 100 fg of cDNA from HeLa total RNA were used in each 20 µl reaction to detect 18S rRNA. 18S rRNA efficiency = 98.6%, r2 = 0.999.
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NEW PRODUCT
SsoAdvanced Universal Supermixes

Affordable, superior performance alternative to SsoFast Supermixes

  • Guaranteed performance under all conditions
  • Use on any real-time PCR instrument without adjusting ROX concentration
SsoAdvanced Universal Supermixes

SsoFast EvaGreen Supermix with Low ROX is a ROX-blended version of our high-performing SsoFast EvaGreen Supermix, which employs the patented Sso7d fusion protein technology* for use in real-time PCR applications. This novel, engineered polymerase, combined with optimized buffer and EvaGreen dye, produces robust qPCR results in less time with increased speed, reliability, and sensitivity under fast thermal cycling conditions.

Key Features and Benefits

  • Fast qPCR results — Sso7d fusion polymerase's stringent hot-start and rapid polymerization kinetics provide consistent qPCR results under fast cycling conditions (approximately 40 min)
  • Preblended with ROX — streamlines use with real-time PCR systems that require ROX for fast runs
  • Minimal PCR inhibition — highly tolerant polymerase and EvaGreen dye exhibit low PCR inhibition and provide better performance with fast cycling protocols
  • Single-copy detection — higher concentrations generate larger fluorescent signals to provide increased sensitivity
  • Broad dynamic range — yields linear results over 6 orders of magnitude on real-time PCR systems

Applications and Uses

  • Dye-based qPCR detection
  • PCR and real-time PCR analysis
  • ROX-dependent real-time PCR detection
  • Gene expression analysis
  • Genotyping studies
  • Genomics-related applications

Related Products

Select the best real-tme PCR supermix for your application:

  • Bio-Rad’s Universal Supermixes can be used in all real-time PCR systems — both ROX-dependent and ROX-independent instruments
  • SsoAdvanced Universal Supermixes feature our patented Sso7d fusion polymerase

Key Features of Bio-Rad Real-Time PCR Supermixes

SsoFast
Supermixes

SsoAdvanced Universal Supermixes

Sso7d fusion protein technology

Yes

Yes

Sensitive detection of low-level targets

Efficiency with difficult target sequences

Specificity

Tolerance to PCR inhibitors

Broad range of reaction conditions

Fast cycling

Instrument compatibility

Select instruments

Any instrument


Related Categories

* U.S. patents 6,627,424; 7,541,170; and 7,560,260.

Product expiration (after shipping date) 12 months
Storage at 4°C (after thawing) Up to 6 months
Customer guarantee (minimum usage time) at –20°C 12 months
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SsoFast™ EvaGreen® Supermix with Low ROX, 200 x 20 µl rxns, 2 ml (2 x 1 ml)

1725210
2 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, ROX passive reference dye, stabilizers, for 200 x 20 µl reactions

1725210 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5210_1268849530.xml 1725210 03/17/10 11:03 AM en_US true internet/product_SKU 2019-05-04 16:19:23 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5210_1268849530.xml /default/main/bio-rad/WORKAREA/default jdl0szap 2017-11-14 07:07:37 true jdl0szap templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5210_1268849530.xml LSR N /default/main/bio-rad en 03/17/20 11:11 AM 1555974592504 2019-05-04 16:19:23 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5210_1268849530.xml 1725210-ssofast-evagreen-supermix-with-low-rox-200-x-20-ul-rxns-2-ml-2-x-1-ml 1 1725210 SsoFast EvaGreen Supermix with Low ROX SsoFast™ EvaGreen<sup>®</sup> Supermix with Low ROX, 200 x 20 µl rxns, 2 ml (2 x 1 ml) 2 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, ROX passive reference dye, stabilizers, for 200 x 20 &micro;l reactions /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/172-5210_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/172-5210_view.jpg ,VN Rick Abruzzo SsoFast EvaGreen Supermix With Low Rox SsoFast EvaGreen Supermix With Low Rox 172-5210, 1725210, ssofast, evagreen, supermix, low, rox, super, master, mix, mastermix, detection, amplification, real-time, pcr, real, time, qpcr, dNTPs, sso7d, fusion, polymerase, mgcl2 03/17/10 11:03 AM 03/17/20 11:11 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Use SsoFast EvaGreen Supermix with Low ROX for fast, highly specific amplification. This supermix is designed for use with real-time PCR systems that have an excitation source and optical configuration that require lower levels of ROX. EvaGreen has similar spectral properties to SYBR<sup>&reg;</sup> Green I and fluorescein.</p> <h2 class="descHeader">Key Features and Benefits</h2> <ul> <li>EvaGreen has lower levels of PCR inhibition than SYBR<sup>&reg;</sup> Green I, and can be used at higher concentrations to generate stronger signals</li> <li>Patented Sso7d fusion polymerase* has a dsDNA-binding domain that stabilizes the polymerase- template complex, increasing processivity and reducing reaction times</li> <li>Antibody-mediated hot start increases specificity since the polymerase is not active until after the initial denaturation</li> </ul> <h2 class="descHeader">Applications and Uses</h2> <ul> <li>Dye-based qPCR detection</li> <li>PCR and real-time PCR analysis</li> <li>Nucleic acid amplification and expression profiling</li> <li>Gene expression analysis</li> <li>Genotyping studies</li> <li>Genomics-related applications</li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" style="width: 500px;" border="0"> <tbody> <tr> <td align="center" valign="top"><strong>Number of 20 &micro;l Reactions</strong></td> <td align="center" valign="top"><strong>Supermix Volume, ml</strong></td> <td align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp;&nbsp;200</td> <td style="padding-left: 70px;" valign="top">&nbsp;&nbsp;2 x 1</td> <td style="padding-left: 40px;" valign="top"><span style="font-size: 11px;">1725210</span></td> </tr> <tr> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp;&nbsp;500</td> <td style="padding-left: 70px;" valign="top">&nbsp;&nbsp;5 x 1</td> <td style="padding-left: 40px;" valign="top"><span style="font-size: 11px;"><a href="/en-us/sku/1725211-ssofast-evagreen-supermix-with-low-rox-500-x-20-ul-rxns-5-ml-5-x-1-ml">1725211</a>**</span></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">1,000</td> <td style="padding-left: 70px;" valign="top">10 x 1</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725212-ssofast-evagreen-supermix-with-low-rox-1-000-x-20-ul-rxns-10-ml-10-x-1-ml"><span style="font-size: 11px;">1725212</span></a><span style="font-size: 11px;">**</span></td> </tr> <tr> <td style="padding-left: 80px;" valign="top">2,000</td> <td style="padding-left: 70px;" valign="top">&nbsp; 1 x 20</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725213-ssofast-evagreen-supermix-with-low-rox-2-000-x-20-ul-rxns-20-ml-1-x-20-ml"><span style="font-size: 11px;">1725213</span></a><span style="font-size: 11px;">**</span></td> </tr> </tbody> </table> <p class="caption">**Not available in Japan</p> <p>See the <a href="/en-us/product/ssofast-evagreen-supermix-with-low-rox">SsoFast EvaGreen Supermix with Low ROX</a> product page for more information and documentation.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>View all <a href="/en-us/category/supermixes-for-pcr-real-time-pcr">Real-Time PCR Supermixes and Kits</a></li> <li><a href="/en-us/product/primepcr-pcr-primers-assays">PrimePCR&trade; PCR Primers &amp; Assays</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/pcr-reagents-qpcr-reagents">PCR and qPCR Reagents</a></li> <li><a href="/en-us/category/real-time-pcr-detection-systems">Real-Time PCR Detection Systems</a></li> <li><a href="/en-us/category/pcr-plastic-consumables">PCR Plastic Consumables</a></li> <li><a href="/en-us/category/pcr-instrument-validation-tools-services">Instrument Validation Tools and Services</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>SsoFast EvaGreen Supermix with Low ROX <a href="/webroot/web/pdf/lsr/literature/10016588.pdf">Product Insert</a></li> <li>Amplification Plastics and Reagents <a href="/webroot/web/pdf/lsr/literature/10022527.pdf">Selection Guide</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/qpcr-real-time-pcr">qPCR/Real-Time PCR</a></li> <li><a href="/en-us/applications-technologies/genomics">Genomics</a></li> </ul> <p class="caption">*U.S. patents 6,627,424; 7,541,170; and 7,560,260</p> /webroot/web/html/lsr/products/SKUs/autopopulated/ 172-5210_1268849530_en.html /webroot/web/html/lsr/products/SKUs/autopopulated//172-5210_1268849530_en.html false $185.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725210&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725210&CartQuote=QT&quantity= 1444 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725210&AddToDefaultWishList=true&quantity= 41106300 185.00 1725210
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SsoFast™ EvaGreen® Supermix with Low ROX, 500 x 20 µl rxns, 5 ml (5 x 1 ml)

1725211
5 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, ROX passive reference dye, stabilizers, for 500 x 20 µl reactions

1725211 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5211_1268849675.xml 1725211 03/17/10 11:11 AM en_US true internet/product_SKU 2019-05-04 16:18:46 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5211_1268849675.xml /default/main/bio-rad/WORKAREA/default jdl0szah 2017-11-14 07:07:37 true jdl0szah templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5211_1268849675.xml LSR N /default/main/bio-rad en 03/17/20 11:14 AM 1555974592546 2019-05-04 16:18:46 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5211_1268849675.xml 1725211-ssofast-evagreen-supermix-with-low-rox-500-x-20-ul-rxns-5-ml-5-x-1-ml 1 1725211 SsoFast EvaGreen Supermix with Low ROX SsoFast™ EvaGreen<sup>®</sup> Supermix with Low ROX, 500 x 20 µl rxns, 5 ml (5 x 1 ml) 5 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, ROX passive reference dye, stabilizers, for 500 x 20 &micro;l reactions /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/172-5211_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/172-5211_view.jpg ,VN Rick Abruzzo SsoFast EvaGreen Supermix With Low Rox SsoFast EvaGreen Supermix With Low Rox 172-5211, 1725211, ssofast, evagreen, supermix, low, rox, super, master, mix, mastermix, detection, amplification, real-time, pcr, real, time, qpcr, dNTPs, sso7d, fusion, polymerase, mgcl2 03/17/10 11:11 AM 03/17/20 11:14 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Use SsoFast EvaGreen Supermix with Low ROX for fast, highly specific amplification. This supermix is designed for use with real-time PCR systems that have an excitation source and optical configuration that require lower levels of ROX. EvaGreen has similar spectral properties to SYBR<sup>&reg;</sup> Green I and fluorescein.</p> <h2 class="descHeader">Key Features and Benefits</h2> <ul> <li>EvaGreen has lower levels of PCR inhibition than SYBR<sup>&reg;</sup> Green I, and can be used at higher concentrations to generate stronger signals</li> <li>Patented Sso7d fusion polymerase* has a dsDNA-binding domain that stabilizes the polymerase- template complex, increasing processivity and reducing reaction times</li> <li>Antibody-mediated hot start increases specificity since the polymerase is not active until after the initial denaturation</li> </ul> <h2 class="descHeader">Applications and Uses</h2> <ul> <li>Dye-based qPCR detection</li> <li>PCR and real-time PCR analysis</li> <li>Nucleic acid amplification and expression profiling</li> <li>Gene expression analysis</li> <li>Genotyping studies</li> <li>Genomics-related applications</li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" style="width: 500px;" border="0"> <tbody> <tr> <td align="center" valign="top"><strong>Number of 20 &micro;l Reactions</strong></td> <td align="center" valign="top"><strong>Supermix Volume, ml</strong></td> <td align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp;&nbsp;200</td> <td style="padding-left: 70px;" valign="top">&nbsp;&nbsp;2 x 1</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725210-ssofast-evagreen-supermix-with-low-rox-200-x-20-ul-rxns-2-ml-2-x-1-ml"><span style="font-size: 11px;">1725210</span></a></td> </tr> <tr> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp;&nbsp;500</td> <td style="padding-left: 70px;" valign="top">&nbsp;&nbsp;5 x 1</td> <td style="padding-left: 40px;" valign="top"><span style="font-size: 11px;">1725211**</span></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">1,000</td> <td style="padding-left: 70px;" valign="top">10 x 1</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725212-ssofast-evagreen-supermix-with-low-rox-1-000-x-20-ul-rxns-10-ml-10-x-1-ml"><span style="font-size: 11px;">1725212</span></a><span style="font-size: 11px;">**</span></td> </tr> <tr> <td style="padding-left: 80px;" valign="top">2,000</td> <td style="padding-left: 70px;" valign="top">&nbsp; 1 x 20</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725213-ssofast-evagreen-supermix-with-low-rox-2-000-x-20-ul-rxns-20-ml-1-x-20-ml"><span style="font-size: 11px;">1725213</span></a><span style="font-size: 11px;">**</span></td> </tr> </tbody> </table> <p class="caption">**Not available in Japan</p> <p>See the <a href="/en-us/product/ssofast-evagreen-supermix-with-low-rox">SsoFast EvaGreen Supermix with Low ROX</a> product page for more information and documentation.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>View all <a href="/en-us/category/supermixes-for-pcr-real-time-pcr">Real-Time PCR Supermixes and Kits</a></li> <li><a href="/en-us/product/primepcr-pcr-primers-assays">PrimePCR&trade; PCR Primers &amp; Assays</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/pcr-reagents-qpcr-reagents">PCR and qPCR Reagents</a></li> <li><a href="/en-us/category/real-time-pcr-detection-systems">Real-Time PCR Detection Systems</a></li> <li><a href="/en-us/category/pcr-plastic-consumables">PCR Plastic Consumables</a></li> <li><a href="/en-us/category/pcr-instrument-validation-tools-services">Instrument Validation Tools and Services</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>SsoFast EvaGreen Supermix with Low ROX <a href="/webroot/web/pdf/lsr/literature/10016588.pdf">Product Insert</a></li> <li>Amplification Plastics and Reagents <a href="/webroot/web/pdf/lsr/literature/10022527.pdf">Selection Guide</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/qpcr-real-time-pcr">qPCR/Real-Time PCR</a></li> <li><a href="/en-us/applications-technologies/genomics">Genomics</a></li> </ul> <p class="caption">*U.S. patents 6,627,424; 7,541,170; and 7,560,260</p> /webroot/web/html/lsr/products/SKUs/autopopulated/ 172-5211_1268849675_en.html /webroot/web/html/lsr/products/SKUs/autopopulated//172-5211_1268849675_en.html false $414.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725211&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725211&CartQuote=QT&quantity= 1444 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725211&AddToDefaultWishList=true&quantity= 41106300 414.00 1725211
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SsoFast™ EvaGreen® Supermix with Low ROX, 1,000 x 20 µl rxns, 10 ml (10 x 1 ml)

1725212
10 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, ROX passive reference dye, stabilizers, for 1,000 x 20 µl reactions

1725212 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5212_1268849794.xml 1725212 03/17/10 11:14 AM en_US true internet/product_SKU 2019-05-04 16:19:04 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5212_1268849794.xml /default/main/bio-rad/WORKAREA/default jdl0szam 2017-11-14 07:07:37 true jdl0szam templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5212_1268849794.xml LSR N /default/main/bio-rad en 03/17/20 11:16 AM 1555974592588 2019-05-04 16:19:04 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5212_1268849794.xml 1725212-ssofast-evagreen-supermix-with-low-rox-1000-x-20-ul-rxns-10-ml-10-x-1-ml 1 1725212 SsoFast EvaGreen Supermix with Low ROX SsoFast™ EvaGreen<sup>®</sup> Supermix with Low ROX, 1,000 x 20 µl rxns, 10 ml (10 x 1 ml) 10 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, ROX passive reference dye, stabilizers, for 1,000 x 20 &micro;l reactions /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/172-5212_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/172-5212_view.jpg ,VN Rick Abruzzo SsoFast EvaGreen Supermix With Low Rox SsoFast EvaGreen Supermix With Low Rox 172-5212, 1725212, ssofast, evagreen, supermix, low rox, super, master, mix, mastermix, detection, amplification, real-time, pcr, real, time, qpcr, dNTPs, sso7d, fusion, polymerase, mgcl2 03/17/10 11:14 AM 03/17/20 11:16 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Use SsoFast EvaGreen Supermix with Low ROX for fast, highly specific amplification. This supermix is designed for use with real-time PCR systems that have an excitation source and optical configuration that require lower levels of ROX. EvaGreen has similar spectral properties to SYBR<sup>&reg;</sup> Green I and fluorescein.</p> <h2 class="descHeader">Key Features and Benefits</h2> <ul> <li>EvaGreen has lower levels of PCR inhibition than SYBR<sup>&reg;</sup> Green I, and can be used at higher concentrations to generate stronger signals</li> <li>Patented Sso7d fusion polymerase* has a dsDNA-binding domain that stabilizes the polymerase- template complex, increasing processivity and reducing reaction times</li> <li>Antibody-mediated hot start increases specificity since the polymerase is not active until after the initial denaturation</li> </ul> <h2 class="descHeader">Applications and Uses</h2> <ul> <li>Dye-based qPCR detection</li> <li>PCR and real-time PCR analysis</li> <li>Nucleic acid amplification and expression profiling</li> <li>Gene expression analysis</li> <li>Genotyping studies</li> <li>Genomics-related applications</li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" style="width: 500px;" border="0"> <tbody> <tr> <td align="center" valign="top"><strong>Number of 20 &micro;l Reactions</strong></td> <td align="center" valign="top"><strong>Supermix Volume, ml</strong></td> <td align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp;&nbsp;200</td> <td style="padding-left: 70px;" valign="top">&nbsp;&nbsp;2 x 1</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725210-ssofast-evagreen-supermix-with-low-rox-200-x-20-ul-rxns-2-ml-2-x-1-ml"><span style="font-size: 11px;">1725210</span></a></td> </tr> <tr> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp;&nbsp;500</td> <td style="padding-left: 70px;" valign="top">&nbsp;&nbsp;5 x 1</td> <td style="padding-left: 40px;" valign="top"><span style="font-size: 11px;"><a href="/en-us/sku/1725211-ssofast-evagreen-supermix-with-low-rox-500-x-20-ul-rxns-5-ml-5-x-1-ml">1725211</a>**</span></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">1,000</td> <td style="padding-left: 70px;" valign="top">10 x 1</td> <td style="padding-left: 40px;" valign="top"><span style="font-size: 11px;">1725212</span><span style="font-size: 11px;">**</span></td> </tr> <tr> <td style="padding-left: 80px;" valign="top">2,000</td> <td style="padding-left: 70px;" valign="top">&nbsp; 1 x 20</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725213-ssofast-evagreen-supermix-with-low-rox-2-000-x-20-ul-rxns-20-ml-1-x-20-ml"><span style="font-size: 11px;">1725213</span></a><span style="font-size: 11px;">**</span></td> </tr> </tbody> </table> <p class="caption">**Not available in Japan</p> <p>See the <a href="/en-us/product/ssofast-evagreen-supermix-with-low-rox">SsoFast EvaGreen Supermix with Low ROX</a> product page for more information and documentation.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>View all <a href="/en-us/category/supermixes-for-pcr-real-time-pcr">Real-Time PCR Supermixes and Kits</a></li> <li><a href="/en-us/product/primepcr-pcr-primers-assays">PrimePCR&trade; PCR Primers &amp; Assays</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/pcr-reagents-qpcr-reagents">PCR and qPCR Reagents</a></li> <li><a href="/en-us/category/real-time-pcr-detection-systems">Real-Time PCR Detection Systems</a></li> <li><a href="/en-us/category/pcr-plastic-consumables">PCR Plastic Consumables</a></li> <li><a href="/en-us/category/pcr-instrument-validation-tools-services">Instrument Validation Tools and Services</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>SsoFast EvaGreen Supermix with Low ROX <a href="/webroot/web/pdf/lsr/literature/10016588.pdf">Product Insert</a></li> <li>Amplification Plastics and Reagents <a href="/webroot/web/pdf/lsr/literature/10022527.pdf">Selection Guide</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/qpcr-real-time-pcr">qPCR/Real-Time PCR</a></li> <li><a href="/en-us/applications-technologies/genomics">Genomics</a></li> </ul> <p class="caption">*U.S. patents 6,627,424; 7,541,170; and 7,560,260</p> /webroot/web/html/lsr/products/SKUs/autopopulated/ 172-5212_1268849794_en.html /webroot/web/html/lsr/products/SKUs/autopopulated//172-5212_1268849794_en.html false $775.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725212&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725212&CartQuote=QT&quantity= 1444 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725212&AddToDefaultWishList=true&quantity= 41106300 775.00 1725212
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SsoFast™ EvaGreen® Supermix with Low ROX, 2,000 x 20 µl rxns, 20 ml (1 x 20 ml)

1725213
20 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, ROX passive reference dye, stabilizers, for 2,000 x 20 µl reactions

1725213 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5213_1268849886.xml 1725213 03/17/10 11:15 AM en_US true internet/product_SKU 2019-05-04 16:19:16 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5213_1268849886.xml /default/main/bio-rad/WORKAREA/default jdl0szaj 2017-11-14 07:07:37 true jdl0szaj templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5213_1268849886.xml LSR N /default/main/bio-rad en 03/17/20 11:18 AM 1555974592631 2019-05-04 16:19:16 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5213_1268849886.xml 1725213-ssofast-evagreen-supermix-with-low-rox-2000-x-20-ul-rxns-20-ml-1-x-20-ml 1 1725213 SsoFast EvaGreen Supermix with Low ROX SsoFast™ EvaGreen<sup>®</sup> Supermix with Low ROX, 2,000 x 20 µl rxns, 20 ml (1 x 20 ml) 20 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, ROX passive reference dye, stabilizers, for 2,000 x 20 &micro;l reactions /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/172-5213_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/172-5213_view.jpg ,VN Rick Abruzzo SsoFast EvaGreen Supermix With Low Rox SsoFast EvaGreen Supermix With Low Rox 172-5213, 1725213, ssofast, evagreen, supermix, low, rox, super, master, mix, mastermix, detection, amplification, real-time, pcr, real, time, qpcr, dNTPs, sso7d, fusion, polymerase, mgcl2 03/17/10 11:15 AM 03/17/20 11:18 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Use SsoFast EvaGreen Supermix with Low ROX for fast, highly specific amplification. This supermix is designed for use with real-time PCR systems that have an excitation source and optical configuration that require lower levels of ROX. EvaGreen has similar spectral properties to SYBR<sup>&reg;</sup> Green I and fluorescein.</p> <h2 class="descHeader">Key Features and Benefits</h2> <ul> <li>EvaGreen has lower levels of PCR inhibition than SYBR<sup>&reg;</sup> Green I, and can be used at higher concentrations to generate stronger signals</li> <li>Patented Sso7d fusion polymerase* has a dsDNA-binding domain that stabilizes the polymerase- template complex, increasing processivity and reducing reaction times</li> <li>Antibody-mediated hot start increases specificity since the polymerase is not active until after the initial denaturation</li> </ul> <h2 class="descHeader">Applications and Uses</h2> <ul> <li>Dye-based qPCR detection</li> <li>PCR and real-time PCR analysis</li> <li>Nucleic acid amplification and expression profiling</li> <li>Gene expression analysis</li> <li>Genotyping studies</li> <li>Genomics-related applications</li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" style="width: 500px;" border="0"> <tbody> <tr> <td align="center" valign="top"><strong>Number of 20 &micro;l Reactions</strong></td> <td align="center" valign="top"><strong>Supermix Volume, ml</strong></td> <td align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp;&nbsp;200</td> <td style="padding-left: 70px;" valign="top">&nbsp;&nbsp;2 x 1</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725210-ssofast-evagreen-supermix-with-low-rox-200-x-20-ul-rxns-2-ml-2-x-1-ml"><span style="font-size: 11px;">1725210</span></a></td> </tr> <tr> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp;&nbsp;500</td> <td style="padding-left: 70px;" valign="top">&nbsp;&nbsp;5 x 1</td> <td style="padding-left: 40px;" valign="top"><span style="font-size: 11px;"><a href="/en-us/sku/1725211-ssofast-evagreen-supermix-with-low-rox-500-x-20-ul-rxns-5-ml-5-x-1-ml">1725211</a>**</span></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">1,000</td> <td style="padding-left: 70px;" valign="top">10 x 1</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725212-ssofast-evagreen-supermix-with-low-rox-1-000-x-20-ul-rxns-10-ml-10-x-1-ml"><span style="font-size: 11px;">1725212</span></a><span style="font-size: 11px;">**</span></td> </tr> <tr> <td style="padding-left: 80px;" valign="top">2,000</td> <td style="padding-left: 70px;" valign="top">&nbsp; 1 x 20</td> <td style="padding-left: 40px;" valign="top"><span style="font-size: 11px;">1725213</span><span style="font-size: 11px;">**</span></td> </tr> </tbody> </table> <p class="caption">**Not available in Japan</p> <p>See the <a href="/en-us/product/ssofast-evagreen-supermix-with-low-rox">SsoFast EvaGreen Supermix with Low ROX</a> product page for more information and documentation.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>View all <a href="/en-us/category/supermixes-for-pcr-real-time-pcr">Real-Time PCR Supermixes and Kits</a></li> <li><a href="/en-us/product/primepcr-pcr-primers-assays">PrimePCR&trade; PCR Primers &amp; Assays</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/pcr-reagents-qpcr-reagents">PCR and qPCR Reagents</a></li> <li><a href="/en-us/category/real-time-pcr-detection-systems">Real-Time PCR Detection Systems</a></li> <li><a href="/en-us/category/pcr-plastic-consumables">PCR Plastic Consumables</a></li> <li><a href="/en-us/category/pcr-instrument-validation-tools-services">Instrument Validation Tools and Services</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>SsoFast EvaGreen Supermix with Low ROX <a href="/webroot/web/pdf/lsr/literature/10016588.pdf">Product Insert</a></li> <li>Amplification Plastics and Reagents <a href="/webroot/web/pdf/lsr/literature/10022527.pdf">Selection Guide</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/qpcr-real-time-pcr">qPCR/Real-Time PCR</a></li> <li><a href="/en-us/applications-technologies/genomics">Genomics</a></li> </ul> <p class="caption">*U.S. patents 6,627,424; 7,541,170; and 7,560,260</p> /webroot/web/html/lsr/products/SKUs/autopopulated/ 172-5213_1268849886_en.html /webroot/web/html/lsr/products/SKUs/autopopulated//172-5213_1268849886_en.html false $1,512.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725213&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725213&CartQuote=QT&quantity= 1444 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725213&AddToDefaultWishList=true&quantity= 41106300 1512.00 1725213
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Number Description Options
10016588 Instruction Manual, SsoFast™ EvaGreen® Supermix With Low ROX, Rev. B Click to download
5919 SsoFast EvaGreen Supermix with Low ROX Flier, Rev B Click to download
6252 Reagent Comparison Guide for Real-Time PCR Brochure, Rev C Click to download [ Add to Cart (Free) ]
7178 Amplification: Real-Time PCR Reagents and Kits Brochure Click to download
6090 Amplification Reagents and Plastics Brochure Click to download [ Add to Cart (Free) ]
6763 Gene Expression Real-Time PCR Workflow Flier Click to download [ Add to Cart (Free) ]

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