
Description
Description
SsoFast EvaGreen Supermix with Low ROX is a ROX-blended version of our high-performing SsoFast EvaGreen Supermix, which employs the patented Sso7d fusion protein technology* for use in real-time PCR applications. This novel, engineered polymerase, combined with optimized buffer and EvaGreen dye, produces robust qPCR results in less time with increased speed, reliability, and sensitivity under fast thermal cycling conditions.
Key Features and Benefits
- Fast qPCR results — Sso7d fusion polymerase's stringent hot-start and rapid polymerization kinetics provide consistent qPCR results under fast cycling conditions (approximately 40 min)
- Preblended with ROX — streamlines use with real-time PCR systems that require ROX for fast runs
- Minimal PCR inhibition — highly tolerant polymerase and EvaGreen dye exhibit low PCR inhibition and provide better performance with fast cycling protocols
- Single-copy detection — higher concentrations generate larger fluorescent signals to provide increased sensitivity
- Broad dynamic range — yields linear results over 6 orders of magnitude on real-time PCR systems
Applications and Uses
- Dye-based qPCR detection
- PCR and real-time PCR analysis
- ROX-dependent real-time PCR detection
- Gene expression analysis
- Genotyping studies
- Genomics-related applications
Related Products
Select the best real-tme PCR supermix for your application:
- Bio-Rad’s Universal Supermixes can be used in all real-time PCR systems — both ROX-dependent and ROX-independent instruments
- SsoAdvanced Universal Supermixes feature our patented Sso7d fusion polymerase
Key Features of Bio-Rad Real-Time PCR Supermixes |
SsoFast |
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Sso7d fusion protein technology |
Yes |
Yes |
Sensitive detection of low-level targets |
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Efficiency with difficult target sequences |
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Specificity |
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Tolerance to PCR inhibitors |
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Broad range of reaction conditions |
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Fast cycling |
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Instrument compatibility |
Select instruments |
Any instrument |
Related Categories
* U.S. patent 7,560,260.
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