SsoFast EvaGreen Supermixes

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SsoFast EvaGreen Supermix

Overview

Sso7d Fusion Protein Technology
Bio-Rad’s patented* Sso7d fusion protein technology was first employed in the iProof™ high fidelity DNA polymerase product line. The unique Sso7d fusion polymerase in the SsoFast™ supermixes enables fast cycling without affecting PCR sensitivity, efficiency, and reproducibility. The dsDNA binding protein, Sso7d, stabilizes the polymerase-template complex, increases processivity, and provides greater speed and reduced reaction times compared to conventional DNA polymerases. Furthermore, this polymerase is significantly more resistant to PCR inhibitors, making the SsoFast  supermixes ideal choices for applications such as direct qPCR, without the need for sample preparation.
 
Fusing the double-stranded DNA binding protein Sso7d to DNA polymerases provides a powerful sliding grip on the replicated DNA.
 
* U.S. patents 6,627,424; 7,541,170; and 7,560,260.
Performance

The unique fusion polymerase in SsoFast EvaGreen supermix delivers extreme speed and generates exceptional qPCR results in less than 30 min. Tenfold serial dilutions of 10 ng to 100 ag of cDNA from human spleen were used in each 20 µl reaction to detect 18S RNA. 18S efficiency = 101.8%, r = 0.997. Total qPCR run time = 29 min. RFU, relative fluorescence units.

Sensitivity

SsoFast EvaGreen supermix provides extreme sensitivity in detection of a single copy of target gene. The ZAP70 gene was amplified and detected from fivefold serial dilutions of 10 ng to 80 pg () and 3.2 pg () of human genomic DNA. ZAP70 efficiency = 102.7%, r = 0.991. Insert shows the standard curve for the various dilutions. RFU, relative fluorescence units.

Reproducibility

Exceptional reproducibility can be achieved with SsoFast EvaGreen supermix. Efficient discrimination and reliable quantification can be obtained from 1.33-fold serial dilutions of input template. The CBP gene was amplified from varying amounts of human genomic DNA (5 ng to 500 pg). From left to right: () 5 ng, 2.83 ng, 1.60 ng, 903 pg, and 511 pg; () 3.76 ng, 2.13 ng, 1.20 ng, and 679 pg. CBP efficiency = 96.5%, r = 0.996. Insert is a magnified view showing robust discrimination and reproducible amplification. RFU, relative fluorescence units.

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NEW PRODUCT
SsoAdvanced Universal Supermixes

Affordable, superior performance alternative to SsoFast Supermixes

  • Guaranteed performance under all conditions
  • Use on any real-time PCR instrument without adjusting ROX concentration
SsoAdvanced Universal Supermixes

SsoFast EvaGreen Supermix utilizes our patented Sso7d fusion protein technology* for high performance in a wide range of qPCR applications. The dsDNA-binding protein Sso7d stabilizes the polymerase-template complex, increasing processivity and reducing reaction times compared with conventional DNA polymerases without affecting PCR sensitivity, efficiency, or reproducibility. EvaGreen formulation provides increased fluorescence compared to SYBR Green.

Key Features and Benefits

  • Fast qPCR results and high performance — instant activation and rapid polymerization kinetics generate exceptional qPCR results in less than 30 min
  • Minimal PCR inhibition — increased resistance to PCR inhibitors ensures maximum efficiency, sensitivity, and reproducibility
  • Single-copy detection — provides high sensitivity with amplification and detection from a single copy of a target gene
  • Robust discrimination and reproducibility — achieves efficient discrimination and reliable quantification from 1.33-fold serial dilutions of template

Applications and Uses

  • Dye-based qPCR detection
  • Gene expression analysis
  • Genotyping studies

Related Products

Select the best real-time PCR supermix for your application:

  • Bio-Rad’s Universal Supermixes can be used in all real-time PCR systems — both ROX-dependent and ROX-independent instruments
  • SsoAdvanced Universal Supermixes feature our patented Sso7d fusion polymerase

Key Features of Bio-Rad Real-Time PCR Supermixes

SsoFast
Supermixes

SsoAdvanced Universal Supermixes

Sso7d fusion protein technology

Yes

Yes

Sensitive detection of low-level targets

Efficiency with difficult target sequences

Specificity

Tolerance to PCR inhibitors

Broad range of reaction conditions

Fast cycling

Instrument compatibility

Select instruments

Any instrument


Related Categories

* U.S. patents 6,627,424; 7,541,170; and 7,560,260.

Product expiration (after shipping date) 12 months
Storage at 4°C (after thawing) Up to 6 months
Customer guarantee (minimum usage time) at –20°C 12 months
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SsoFast™ EvaGreen® Supermix, 200 x 20 µl rxns, 2 ml (2 x 1 ml)

1725200
2 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 200 x 20 µl reactions

1725200 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/172-5200_0921120537964.xml 1725200 09/21/09 12:05 PM en_US true internet/product_SKU 2019-05-04 16:18:55 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/172-5200_0921120537964.xml /default/main/bio-rad/WORKAREA/default jcun050u 2017-11-14 07:07:38 true jcun050u templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/172-5200_0921120537964.xml LSR N /default/main/bio-rad en 12/31/37 11:59 PM 1556922204101 2019-05-04 16:18:55 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/172-5200_0921120537964.xml 1725200-ssofast-evagreen-supermix-200-x-20-ul-rxns-2-ml-2-x-1-ml 1 1725200 SsoFast EvaGreen Supermix SsoFast™ EvaGreen<sup>®</sup> Supermix, 200 x 20 µl rxns, 2 ml (2 x 1 ml) 2 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, EvaGreen dye, stabilizers, for 200 x 20 &micro;l reactions 2 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, EvaGreen dye, stabilizers, for 200 x 20 &micro;l reactions 2 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, EvaGreen dye, stabilizers, for 200 x 20 &micro;l reactions /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/172-5200_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/172-5200_view.jpg /webroot/web/images/lsr/products/amplification_pcr/product_detail/global/lsr_ssofast_evagreen_supermix.jpg ,VN RepEx 172-5200 2 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 200 x 20 µl reactions 172-5200, 1725200, ssofast, evagreen, supermix, super, master, mix, mastermix, detection, amplification, real-time, pcr, real, time, qpcr, dNTPs, sso7d, fusion, polymerase, mgcl2 09/21/09 12:05 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/Real-Time-PCR-Detection-System N 2 <p>Use SsoFast EvaGreen Supermix for effective performance in a wide range of qPCR applications. Our patented Sso7d fusion polymerase* uses the Sso7d DNA-binding protein to stabilize the polymerase-template complex, increasing processivity and providing greater speed and reduced reaction times compared to conventional DNA polymerases, without affecting PCR sensitivity, efficiency, or reproducibility.</p> <h2 class="descHeader">Key Features and Benefits</h2> <ul> <li>Fast qPCR results (in less than 30 min) and high performance</li> <li>Minimal inhibition of PCR ensures maximum efficiency, sensitivity, and reproducibility</li> <li>Single copy detection of target genes</li> <li>Robust discrimination and reproducibility</li> </ul> <p><strong> Applications and Uses</strong></p> <ul> <li>Dye-based qPCR detection</li> <li>PCR and real-time PCR analysis</li> <li>Nucleic acid amplification and expression profiling</li> <li>Gene expression analysis</li> <li>Genotyping studies</li> <li>Genomics-related applications</li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" style="width: 500px;" border="0"> <tbody> <tr> <td align="center" valign="top"><strong>Number of 20 &micro;l Reactions</strong></td> <td align="center" valign="top"><strong>Supermix Volume, ml</strong></td> <td align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp;&nbsp;200</td> <td style="padding-left: 70px;" valign="top">&nbsp; 2 (2 x 1 ml)</td> <td style="padding-left: 40px;" valign="top"><span style="font-size: 11px;">1725200</span></td> </tr> <tr> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp;&nbsp;500</td> <td style="padding-left: 70px;" valign="top">&nbsp; 5 (5 x 1 ml)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725201-ssofast-evagreen-supermix-500-x-20-ul-rxns-5-ml-5-x-1-ml"><span style="font-size: 11px;">1725201</span></a></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">1,000</td> <td style="padding-left: 70px;" valign="top">10 (10 x 1 ml)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725202-ssofast-evagreen-supermix-1-000-x-20-ul-rxns-10-ml-10-x-1-ml"><span style="font-size: 11px;">1725202</span></a></td> </tr> <tr> <td style="padding-left: 80px;" valign="top">2,000</td> <td style="padding-left: 70px;" valign="top">&nbsp; 20 (1 x 20 ml)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725203-ssofast-evagreen-supermix-2-000-x-20-ul-rxns-20-ml-1-x-20-ml"><span style="font-size: 11px;">1725203</span></a></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">2,500</td> <td style="padding-left: 70px;" valign="top">25 (5 x 5 ml)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725204-ssofast-evagreen-supermix-2-500-x-20-ul-rxns-25-ml-5-x-5-ml"><span style="font-size: 11px;">1725204</span></a></td> </tr> <tr> <td style="padding-left: 80px;" valign="top">5,000</td> <td style="padding-left: 70px;" valign="top">&nbsp; 50 (10 x 5 ml)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725205-ssofast-evagreen-supermix-5-000-x-20-ul-rxns-50-ml-10-x-5-ml"><span style="font-size: 11px;">1725205</span></a></td> </tr> </tbody> </table> <br /> <p>See the <a href="/en-us/product/ssofast-evagreen-supermixes">SsoFast EvaGreen Supermix</a> product page for more information and documentation.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>View all <a href="/en-us/category/supermixes-for-pcr-real-time-pcr">Real-Time PCR Supermixes and Kits</a></li> <li><a href="/en-us/product/primepcr-pcr-primers-assays">PrimePCR&trade; PCR Primers &amp; Assays</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/pcr-reagents-qpcr-reagents">PCR and qPCR Reagents</a></li> <li><a href="/en-us/category/real-time-pcr-detection-systems">Real-Time PCR Detection Systems</a></li> <li><a href="/en-us/category/pcr-plastic-consumables">PCR Plastic Consumables</a></li> <li><a href="/en-us/category/pcr-instrument-validation-tools-services">Instrument Validation Tools and Services</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>SsoFast EvaGreen Supermix <a href="/webroot/web/pdf/lsr/literature/Bulletin_10014647.pdf">Product Insert</a></li> <li>Amplification Plastics and Reagents <a href="/webroot/web/pdf/lsr/literature/10022527.pdf">Selection Guide</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/qpcr-real-time-pcr">qPCR/Real-Time PCR</a></li> <li><a href="/en-us/applications-technologies/genomics">Genomics</a></li> </ul> <p class="caption">*U.S. patents 6,627,424; 7,541,170; and 7,560,260</p> /webroot/web/html/lsr/products/SKUs/autopopulated/ 172-5200_0921120537964_en.html /webroot/web/html/lsr/products/SKUs/autopopulated//172-5200_0921120537964_en.html false $197.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725200&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725200&CartQuote=QT&quantity= 1442 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725200&AddToDefaultWishList=true&quantity= 24121500 197.00 1725200
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SsoFast™ EvaGreen® Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)

1725201
5 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 500 x 20 µl reactions

1725201 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/172-5201_0921120538246.xml 1725201 09/21/09 12:05 PM en_US true internet/product_SKU 2019-05-04 16:19:24 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/172-5201_0921120538246.xml /default/main/bio-rad/WORKAREA/default jcun04zo 2017-11-14 07:07:38 true jcun04zo templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/172-5201_0921120538246.xml LSR N /default/main/bio-rad en 12/31/37 11:59 PM 1556922204143 2019-05-04 16:19:24 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/172-5201_0921120538246.xml 1725201-ssofast-evagreen-supermix-500-x-20-ul-rxns-5-ml-5-x-1-ml 1 1725201 SsoFast EvaGreen Supermix SsoFast™ EvaGreen<sup>®</sup> Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml) 5 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, EvaGreen dye, stabilizers, for 500 x 20 &micro;l reactions 5 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, EvaGreen dye, stabilizers, for 500 x 20 &micro;l reactions 5 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, EvaGreen dye, stabilizers, for 500 x 20 &micro;l reactions /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/172-5201_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/172-5201_view.jpg /webroot/web/images/lsr/products/amplification_pcr/product_detail/global/172-5201_view.jpg ,VN RepEx 172-5201 5 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, EvaGreen dye, stabilizers, for 500 x 20 &micro;l reactions 172-5201, 1725201, ssofast, evagreen, supermix, super, master, mix, mastermix, detection, amplification, real-time, pcr, real, time, qpcr, dNTPs, sso7d, fusion, polymerase, mgcl2 09/21/09 12:05 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/Real-Time-PCR-Detection-System N 2 <p>Use SsoFast EvaGreen Supermix for effective performance in a wide range of qPCR applications. Our patented Sso7d fusion polymerase* uses the Sso7d DNA-binding protein to stabilize the polymerase-template complex, increasing processivity and providing greater speed and reduced reaction times compared to conventional DNA polymerases, without affecting PCR sensitivity, efficiency, or reproducibility.</p> <h2 class="descHeader">Key Features and Benefits</h2> <ul> <li>Fast qPCR results (in less than 30 min) and high performance</li> <li>Minimal inhibition of PCR ensures maximum efficiency, sensitivity, and reproducibility</li> <li>Single copy detection of target genes</li> <li>Robust discrimination and reproducibility</li> </ul> <p><strong> Applications and Uses</strong></p> <ul> <li>Dye-based qPCR detection</li> <li>PCR and real-time PCR analysis</li> <li>Nucleic acid amplification and expression profiling</li> <li>Gene expression analysis</li> <li>Genotyping studies</li> <li>Genomics-related applications</li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" style="width: 500px;" border="0"> <tbody> <tr> <td align="center" valign="top"><strong>Number of 20 &micro;l Reactions</strong></td> <td align="center" valign="top"><strong>Supermix Volume, ml</strong></td> <td align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp;&nbsp;200</td> <td style="padding-left: 70px;" valign="top">&nbsp; 2 (2 x 1 ml)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725200-ssofast-evagreen-supermix-200-x-20-ul-rxns-2-ml-2-x-1-ml"><span style="font-size: 11px;">1725200</span></a></td> </tr> <tr> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp;&nbsp;500</td> <td style="padding-left: 70px;" valign="top">&nbsp; 5 (5 x 1 ml)</td> <td style="padding-left: 40px;" valign="top"><span style="font-size: 11px;">1725201</span></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">1,000</td> <td style="padding-left: 70px;" valign="top">10 (10 x 1 ml)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725202-ssofast-evagreen-supermix-1-000-x-20-ul-rxns-10-ml-10-x-1-ml"><span style="font-size: 11px;">1725202</span></a></td> </tr> <tr> <td style="padding-left: 80px;" valign="top">2,000</td> <td style="padding-left: 70px;" valign="top">&nbsp; 20 (1 x 20 ml)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725203-ssofast-evagreen-supermix-2-000-x-20-ul-rxns-20-ml-1-x-20-ml"><span style="font-size: 11px;">1725203</span></a></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">2,500</td> <td style="padding-left: 70px;" valign="top">25 (5 x 5 ml)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725204-ssofast-evagreen-supermix-2-500-x-20-ul-rxns-25-ml-5-x-5-ml"><span style="font-size: 11px;">1725204</span></a></td> </tr> <tr> <td style="padding-left: 80px;" valign="top">5,000</td> <td style="padding-left: 70px;" valign="top">&nbsp; 50 (10 x 5 ml)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725205-ssofast-evagreen-supermix-5-000-x-20-ul-rxns-50-ml-10-x-5-ml"><span style="font-size: 11px;">1725205</span></a></td> </tr> </tbody> </table> <br /> <p>See the <a href="/en-us/product/ssofast-evagreen-supermixes">SsoFast EvaGreen Supermix</a> product page for more information and documentation.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>View all <a href="/en-us/category/supermixes-for-pcr-real-time-pcr">Real-Time PCR Supermixes and Kits</a></li> <li><a href="/en-us/product/primepcr-pcr-primers-assays">PrimePCR&trade; PCR Primers &amp; Assays</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/pcr-reagents-qpcr-reagents">PCR and qPCR Reagents</a></li> <li><a href="/en-us/category/real-time-pcr-detection-systems">Real-Time PCR Detection Systems</a></li> <li><a href="/en-us/category/pcr-plastic-consumables">PCR Plastic Consumables</a></li> <li><a href="/en-us/category/pcr-instrument-validation-tools-services">Instrument Validation Tools and Services</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>SsoFast EvaGreen Supermix <a href="/webroot/web/pdf/lsr/literature/Bulletin_10014647.pdf">Product Insert</a></li> <li>Amplification Plastics and Reagents <a href="/webroot/web/pdf/lsr/literature/10022527.pdf">Selection Guide</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/qpcr-real-time-pcr">qPCR/Real-Time PCR</a></li> <li><a href="/en-us/applications-technologies/genomics">Genomics</a></li> </ul> <p class="caption">*U.S. patents 6,627,424; 7,541,170; and 7,560,260</p> /webroot/web/html/lsr/products/SKUs/autopopulated/ 172-5201_0921120538246_en.html /webroot/web/html/lsr/products/SKUs/autopopulated//172-5201_0921120538246_en.html false $435.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725201&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725201&CartQuote=QT&quantity= 1442 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725201&AddToDefaultWishList=true&quantity= 24121500 435.00 1725201
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SsoFast™ EvaGreen® Supermix, 1,000 x 20 µl rxns, 10 ml (10 x 1 ml)

1725202
10 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 1,000 x 20 µl reactions

1725202 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/172-5202_0921120538017.xml 1725202 09/21/09 12:05 PM en_US true internet/product_SKU 2019-05-04 16:18:46 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/172-5202_0921120538017.xml /default/main/bio-rad/WORKAREA/default jcun04zu 2017-11-14 07:07:38 true jcun04zu templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/172-5202_0921120538017.xml LSR N /default/main/bio-rad en 12/31/37 11:59 PM 1556922204185 2019-05-04 16:18:46 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/172-5202_0921120538017.xml 1725202-ssofast-evagreen-supermix-1000-x-20-ul-rxns-10-ml-10-x-1-ml 1 1725202 SsoFast EvaGreen Supermix SsoFast™ EvaGreen<sup>®</sup> Supermix, 1,000 x 20 µl rxns, 10 ml (10 x 1 ml) 10 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, EvaGreen dye, stabilizers, for 1,000 x 20 &micro;l reactions 10 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, EvaGreen dye, stabilizers, for 1,000 x 20 &micro;l reactions 10 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, EvaGreen dye, stabilizers, for 1,000 x 20 &micro;l reactions /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/172-5202_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/172-5202_view.jpg /webroot/web/images/lsr/products/amplification_pcr/product_detail/global/172-5202_view.jpg ,VN RepEx 172-5202 10 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, EvaGreen dye, stabilizers, for 1,000 x 20 &micro;l reactions 172-5202, 1725202, ssofast, evagreen, supermix, super, master, mix, mastermix, detection, amplification, real-time, pcr, real, time, qpcr, dNTPs, sso7d, fusion, polymerase, mgcl2 09/21/09 12:05 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/Real-Time-PCR-Detection-System N 0 <p>Use SsoFast EvaGreen Supermix for effective performance in a wide range of qPCR applications. Our patented Sso7d fusion polymerase* uses the Sso7d DNA-binding protein to stabilize the polymerase-template complex, increasing processivity and providing greater speed and reduced reaction times compared to conventional DNA polymerases, without affecting PCR sensitivity, efficiency, or reproducibility.</p> <h2 class="descHeader">Key Features and Benefits</h2> <ul> <li>Fast qPCR results (in less than 30 min) and high performance</li> <li>Minimal inhibition of PCR ensures maximum efficiency, sensitivity, and reproducibility</li> <li>Single copy detection of target genes</li> <li>Robust discrimination and reproducibility</li> </ul> <p><strong> Applications and Uses</strong></p> <ul> <li>Dye-based qPCR detection</li> <li>PCR and real-time PCR analysis</li> <li>Nucleic acid amplification and expression profiling</li> <li>Gene expression analysis</li> <li>Genotyping studies</li> <li>Genomics-related applications</li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" style="width: 500px;" border="0"> <tbody> <tr> <td align="center" valign="top"><strong>Number of 20 &micro;l Reactions</strong></td> <td align="center" valign="top"><strong>Supermix Volume, ml</strong></td> <td align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp;&nbsp;200</td> <td style="padding-left: 70px;" valign="top">&nbsp; 2 (2 x 1 ml)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725200-ssofast-evagreen-supermix-200-x-20-ul-rxns-2-ml-2-x-1-ml"><span style="font-size: 11px;">1725200</span></a></td> </tr> <tr> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp;&nbsp;500</td> <td style="padding-left: 70px;" valign="top">&nbsp; 5 (5 x 1 ml)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725201-ssofast-evagreen-supermix-500-x-20-ul-rxns-5-ml-5-x-1-ml"><span style="font-size: 11px;">1725201</span></a></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">1,000</td> <td style="padding-left: 70px;" valign="top">10 (10 x 1 ml)</td> <td style="padding-left: 40px;" valign="top"><span style="font-size: 11px;">1725202</span></td> </tr> <tr> <td style="padding-left: 80px;" valign="top">2,000</td> <td style="padding-left: 70px;" valign="top">&nbsp; 20 (1 x 20 ml)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725203-ssofast-evagreen-supermix-2-000-x-20-ul-rxns-20-ml-1-x-20-ml"><span style="font-size: 11px;">1725203</span></a></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">2,500</td> <td style="padding-left: 70px;" valign="top">25 (5 x 5 ml)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725204-ssofast-evagreen-supermix-2-500-x-20-ul-rxns-25-ml-5-x-5-ml"><span style="font-size: 11px;">1725204</span></a></td> </tr> <tr> <td style="padding-left: 80px;" valign="top">5,000</td> <td style="padding-left: 70px;" valign="top">&nbsp; 50 (10 x 5 ml)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725205-ssofast-evagreen-supermix-5-000-x-20-ul-rxns-50-ml-10-x-5-ml"><span style="font-size: 11px;">1725205</span></a></td> </tr> </tbody> </table> <br /> <p>See the <a href="/en-us/product/ssofast-evagreen-supermixes">SsoFast EvaGreen Supermix</a> product page for more information and documentation.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>View all <a href="/en-us/category/supermixes-for-pcr-real-time-pcr">Real-Time PCR Supermixes and Kits</a></li> <li><a href="/en-us/product/primepcr-pcr-primers-assays">PrimePCR&trade; PCR Primers &amp; Assays</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/pcr-reagents-qpcr-reagents">PCR and qPCR Reagents</a></li> <li><a href="/en-us/category/real-time-pcr-detection-systems">Real-Time PCR Detection Systems</a></li> <li><a href="/en-us/category/pcr-plastic-consumables">PCR Plastic Consumables</a></li> <li><a href="/en-us/category/pcr-instrument-validation-tools-services">Instrument Validation Tools and Services</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>SsoFast EvaGreen Supermix <a href="/webroot/web/pdf/lsr/literature/Bulletin_10014647.pdf">Product Insert</a></li> <li>Amplification Plastics and Reagents <a href="/webroot/web/pdf/lsr/literature/10022527.pdf">Selection Guide</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/qpcr-real-time-pcr">qPCR/Real-Time PCR</a></li> <li><a href="/en-us/applications-technologies/genomics">Genomics</a></li> </ul> <p class="caption">*U.S. patents 6,627,424; 7,541,170; and 7,560,260</p> /webroot/web/html/lsr/products/SKUs/autopopulated/ 172-5202_0921120538017_en.html /webroot/web/html/lsr/products/SKUs/autopopulated//172-5202_0921120538017_en.html false $817.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725202&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725202&CartQuote=QT&quantity= 1442 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725202&AddToDefaultWishList=true&quantity= 41106300 817.00 1725202
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SsoFast™ EvaGreen® Supermix, 2,000 x 20 µl rxns, 20 ml (1 x 20 ml)

1725203
20 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 2,000 x 20 µl reactions

1725203 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/172-5203_0921120538458.xml 1725203 09/21/09 12:05 PM en_US true internet/product_SKU 2019-05-04 16:18:53 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/172-5203_0921120538458.xml /default/main/bio-rad/WORKAREA/default jcun050o 2017-11-14 07:07:38 true jcun050o templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/172-5203_0921120538458.xml LSR N /default/main/bio-rad en 12/31/37 11:59 PM 1556922204227 2019-05-04 16:18:53 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/172-5203_0921120538458.xml 1725203-ssofast-evagreen-supermix-2000-x-20-ul-rxns-20-ml-1-x-20-ml 1 1725203 SsoFast EvaGreen Supermix SsoFast™ EvaGreen<sup>®</sup> Supermix, 2,000 x 20 µl rxns, 20 ml (1 x 20 ml) 20 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, EvaGreen dye, stabilizers, for 2,000 x 20 &micro;l reactions 20 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, EvaGreen dye, stabilizers, for 2,000 x 20 &micro;l reactions 20 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, EvaGreen dye, stabilizers, for 2,000 x 20 &micro;l reactions /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/172-5203_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/172-5203_view.jpg /webroot/web/images/lsr/products/amplification_pcr/product_detail/global/172-5203_feature.jpg ,VN RepEx 172-5203 20 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, EvaGreen dye, stabilizers, for 2,000 x 20 &micro;l reactions 172-5203, 1725203, ssofast, evagreen, supermix, super, master, mix, mastermix, detection, amplification, real-time, pcr, real, time, qpcr, dNTPs, sso7d, fusion, polymerase, mgcl2 09/21/09 12:05 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/Real-Time-PCR-Detection-System N 0 <p>Use SsoFast EvaGreen Supermix for effective performance in a wide range of qPCR applications. Our patented Sso7d fusion polymerase* uses the Sso7d DNA-binding protein to stabilize the polymerase-template complex, increasing processivity and providing greater speed and reduced reaction times compared to conventional DNA polymerases, without affecting PCR sensitivity, efficiency, or reproducibility.</p> <h2 class="descHeader">Key Features and Benefits</h2> <ul> <li>Fast qPCR results (in less than 30 min) and high performance</li> <li>Minimal inhibition of PCR ensures maximum efficiency, sensitivity, and reproducibility</li> <li>Single copy detection of target genes</li> <li>Robust discrimination and reproducibility</li> </ul> <p><strong> Applications and Uses</strong></p> <ul> <li>Dye-based qPCR detection</li> <li>PCR and real-time PCR analysis</li> <li>Nucleic acid amplification and expression profiling</li> <li>Gene expression analysis</li> <li>Genotyping studies</li> <li>Genomics-related applications</li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" style="width: 500px;" border="0"> <tbody> <tr> <td align="center" valign="top"><strong>Number of 20 &micro;l Reactions</strong></td> <td align="center" valign="top"><strong>Supermix Volume, ml</strong></td> <td align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp;&nbsp;200</td> <td style="padding-left: 70px;" valign="top">&nbsp; 2 (2 x 1 ml)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725200-ssofast-evagreen-supermix-200-x-20-ul-rxns-2-ml-2-x-1-ml"><span style="font-size: 11px;">1725200</span></a></td> </tr> <tr> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp;&nbsp;500</td> <td style="padding-left: 70px;" valign="top">&nbsp; 5 (5 x 1 ml)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725201-ssofast-evagreen-supermix-500-x-20-ul-rxns-5-ml-5-x-1-ml"><span style="font-size: 11px;">1725201</span></a></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">1,000</td> <td style="padding-left: 70px;" valign="top">10 (10 x 1 ml)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725202-ssofast-evagreen-supermix-1-000-x-20-ul-rxns-10-ml-10-x-1-ml"><span style="font-size: 11px;">1725202</span></a></td> </tr> <tr> <td style="padding-left: 80px;" valign="top">2,000</td> <td style="padding-left: 70px;" valign="top">&nbsp; 20 (1 x 20 ml)</td> <td style="padding-left: 40px;" valign="top"><span style="font-size: 11px;">1725203</span></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">2,500</td> <td style="padding-left: 70px;" valign="top">25 (5 x 5 ml)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725204-ssofast-evagreen-supermix-2-500-x-20-ul-rxns-25-ml-5-x-5-ml"><span style="font-size: 11px;">1725204</span></a></td> </tr> <tr> <td style="padding-left: 80px;" valign="top">5,000</td> <td style="padding-left: 70px;" valign="top">&nbsp; 50 (10 x 5 ml)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725205-ssofast-evagreen-supermix-5-000-x-20-ul-rxns-50-ml-10-x-5-ml"><span style="font-size: 11px;">1725205</span></a></td> </tr> </tbody> </table> <br /> <p>See the <a href="/en-us/product/ssofast-evagreen-supermixes">SsoFast EvaGreen Supermix</a> product page for more information and documentation.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>View all <a href="/en-us/category/supermixes-for-pcr-real-time-pcr">Real-Time PCR Supermixes and Kits</a></li> <li><a href="/en-us/product/primepcr-pcr-primers-assays">PrimePCR&trade; PCR Primers &amp; Assays</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/pcr-reagents-qpcr-reagents">PCR and qPCR Reagents</a></li> <li><a href="/en-us/category/real-time-pcr-detection-systems">Real-Time PCR Detection Systems</a></li> <li><a href="/en-us/category/pcr-plastic-consumables">PCR Plastic Consumables</a></li> <li><a href="/en-us/category/pcr-instrument-validation-tools-services">Instrument Validation Tools and Services</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>SsoFast EvaGreen Supermix <a href="/webroot/web/pdf/lsr/literature/Bulletin_10014647.pdf">Product Insert</a></li> <li>Amplification Plastics and Reagents <a href="/webroot/web/pdf/lsr/literature/10022527.pdf">Selection Guide</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/qpcr-real-time-pcr">qPCR/Real-Time PCR</a></li> <li><a href="/en-us/applications-technologies/genomics">Genomics</a></li> </ul> <p class="caption">*U.S. patents 6,627,424; 7,541,170; and 7,560,260</p> /webroot/web/html/lsr/products/SKUs/autopopulated/ 172-5203_0921120538458_en.html /webroot/web/html/lsr/products/SKUs/autopopulated//172-5203_0921120538458_en.html false $1,602.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725203&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725203&CartQuote=QT&quantity= 1442 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725203&AddToDefaultWishList=true&quantity= 41106300 1602.00 1725203
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SsoFast™ EvaGreen® Supermix, 2,500 x 20 µl rxns, 25 ml (5 x 5 ml)

1725204
25 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 2,500 x 20 µl reactions

1725204 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5204_1289949085.xml 1725204 11/16/10 02:40 PM en_US true internet/product_SKU 2019-05-04 16:19:27 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5204_1289949085.xml /default/main/bio-rad/WORKAREA/default jcv375a8 2017-11-14 07:07:36 true jcv375a8 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5204_1289949085.xml LSR N /default/main/bio-rad en 11/16/20 03:10 PM 1555974592377 2019-05-04 16:19:27 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5204_1289949085.xml 1725204-ssofast-evagreen-supermix-2500-x-20-ul-rxns-25-ml-5-x-5-ml 1 1725204 SsoFast EvaGreen Supermix SsoFast™ EvaGreen<sup>®</sup> Supermix, 2,500 x 20 µl rxns, 25 ml (5 x 5 ml) 25 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, EvaGreen dye, stabilizers, for 2,500 x 20 µl reactions 25 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, EvaGreen dye, stabilizers, for 2,500 x 20 µl reactions /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/172-5204_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/172-5204_view.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_feature/global/172-5204_feature.jpg ,VN Molly Gregg 172-5204, 1725204, ssofast, evagreen, supermix, super, master, mix, mastermix, detection, amplification, real-time, pcr, real, time, qpcr, dNTPs, sso7d, fusion, polymerase, mgcl2 11/16/10 02:40 PM 11/16/20 03:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Use SsoFast EvaGreen Supermix for effective performance in a wide range of qPCR applications. Our patented Sso7d fusion polymerase* uses the Sso7d DNA-binding protein to stabilize the polymerase-template complex, increasing processivity and providing greater speed and reduced reaction times compared to conventional DNA polymerases, without affecting PCR sensitivity, efficiency, or reproducibility.</p> <h2 class="descHeader">Key Features and Benefits</h2> <ul> <li>Fast qPCR results (in less than 30 min) and high performance</li> <li>Minimal inhibition of PCR ensures maximum efficiency, sensitivity, and reproducibility</li> <li>Single copy detection of target genes</li> <li>Robust discrimination and reproducibility</li> </ul> <p><strong> Applications and Uses</strong></p> <ul> <li>Dye-based qPCR detection</li> <li>PCR and real-time PCR analysis</li> <li>Nucleic acid amplification and expression profiling</li> <li>Gene expression analysis</li> <li>Genotyping studies</li> <li>Genomics-related applications</li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" style="width: 500px;" border="0"> <tbody> <tr> <td align="center" valign="top"><strong>Number of 20 &micro;l Reactions</strong></td> <td align="center" valign="top"><strong>Supermix Volume, ml</strong></td> <td align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp;&nbsp;200</td> <td style="padding-left: 70px;" valign="top">&nbsp; 2 (2 x 1 ml)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725200-ssofast-evagreen-supermix-200-x-20-ul-rxns-2-ml-2-x-1-ml"><span style="font-size: 11px;">1725200</span></a></td> </tr> <tr> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp;&nbsp;500</td> <td style="padding-left: 70px;" valign="top">&nbsp; 5 (5 x 1 ml)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725201-ssofast-evagreen-supermix-500-x-20-ul-rxns-5-ml-5-x-1-ml"><span style="font-size: 11px;">1725201</span></a></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">1,000</td> <td style="padding-left: 70px;" valign="top">10 (10 x 1 ml)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725202-ssofast-evagreen-supermix-1-000-x-20-ul-rxns-10-ml-10-x-1-ml"><span style="font-size: 11px;">1725202</span></a></td> </tr> <tr> <td style="padding-left: 80px;" valign="top">2,000</td> <td style="padding-left: 70px;" valign="top">&nbsp; 20 (1 x 20 ml)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725203-ssofast-evagreen-supermix-2-000-x-20-ul-rxns-20-ml-1-x-20-ml"><span style="font-size: 11px;">1725203</span></a></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">2,500</td> <td style="padding-left: 70px;" valign="top">25 (5 x 5 ml)</td> <td style="padding-left: 40px;" valign="top"><span style="font-size: 11px;">1725204</span></td> </tr> <tr> <td style="padding-left: 80px;" valign="top">5,000</td> <td style="padding-left: 70px;" valign="top">&nbsp; 50 (10 x 5 ml)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725205-ssofast-evagreen-supermix-5-000-x-20-ul-rxns-50-ml-10-x-5-ml"><span style="font-size: 11px;">1725205</span></a></td> </tr> </tbody> </table> <br /> <p>See the <a href="/en-us/product/ssofast-evagreen-supermixes">SsoFast EvaGreen Supermix</a> product page for more information and documentation.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>View all <a href="/en-us/category/supermixes-for-pcr-real-time-pcr">Real-Time PCR Supermixes and Kits</a></li> <li><a href="/en-us/product/primepcr-pcr-primers-assays">PrimePCR&trade; PCR Primers &amp; Assays</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/pcr-reagents-qpcr-reagents">PCR and qPCR Reagents</a></li> <li><a href="/en-us/category/real-time-pcr-detection-systems">Real-Time PCR Detection Systems</a></li> <li><a href="/en-us/category/pcr-plastic-consumables">PCR Plastic Consumables</a></li> <li><a href="/en-us/category/pcr-instrument-validation-tools-services">Instrument Validation Tools and Services</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>SsoFast EvaGreen Supermix <a href="/webroot/web/pdf/lsr/literature/Bulletin_10014647.pdf">Product Insert</a></li> <li>Amplification Plastics and Reagents <a href="/webroot/web/pdf/lsr/literature/10022527.pdf">Selection Guide</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/qpcr-real-time-pcr">qPCR/Real-Time PCR</a></li> <li><a href="/en-us/applications-technologies/genomics">Genomics</a></li> </ul> <p class="caption">*U.S. patents 6,627,424; 7,541,170; and 7,560,260</p> /webroot/web/html/lsr/products/SKUs/autopopulated 172-5204.html /webroot/web/html/lsr/products/SKUs/autopopulated/172-5204.html &nbsp; false $1,894.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725204&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725204&CartQuote=QT&quantity= 1442 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725204&AddToDefaultWishList=true&quantity= 41106300 1894.00 1725204
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SsoFast™ EvaGreen® Supermix, 5,000 x 20 µl rxns, 50 ml (10 x 5 ml)

1725205
50 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 5,000 x 20 µl reactions

1725205 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5205_1289949361.xml 1725205 11/16/10 03:12 PM en_US true internet/product_SKU 2019-05-04 16:19:23 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5205_1289949361.xml /default/main/bio-rad/WORKAREA/default jcv375ab 2017-11-14 07:07:37 true jcv375ab templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5205_1289949361.xml LSR N /default/main/bio-rad en 11/16/20 03:15 PM 1555974592462 2019-05-04 16:19:23 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5205_1289949361.xml 1725205-ssofast-evagreen-supermix-5000-x-20-ul-rxns-50-ml-10-x-5-ml 1 1725205 SsoFast EvaGreen Supermix SsoFast™ EvaGreen<sup>®</sup> Supermix, 5,000 x 20 µl rxns, 50 ml (10 x 5 ml) 50 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, EvaGreen dye, stabilizers, for 5,000 x 20 µl reactions 50 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, EvaGreen dye, stabilizers, for 5,000 x 20 µl reactions /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/172-5205_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/172-5205_view.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_feature/global/172-5205_feature.jpg ,VN Molly Gregg 172-5205, 1725205, ssofast, evagreen, supermix, super, master, mix, mastermix, detection, amplification, real-time, pcr, real, time, qpcr, dNTPs, sso7d, fusion, polymerase, mgcl2 11/16/10 03:12 PM 11/16/20 03:15 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Use SsoFast EvaGreen Supermix for effective performance in a wide range of qPCR applications. Our patented Sso7d fusion polymerase* uses the Sso7d DNA-binding protein to stabilize the polymerase-template complex, increasing processivity and providing greater speed and reduced reaction times compared to conventional DNA polymerases, without affecting PCR sensitivity, efficiency, or reproducibility.</p> <h2 class="descHeader">Key Features and Benefits</h2> <ul> <li>Fast qPCR results (in less than 30 min) and high performance</li> <li>Minimal inhibition of PCR ensures maximum efficiency, sensitivity, and reproducibility</li> <li>Single copy detection of target genes</li> <li>Robust discrimination and reproducibility</li> </ul> <p><strong> Applications and Uses</strong></p> <ul> <li>Dye-based qPCR detection</li> <li>PCR and real-time PCR analysis</li> <li>Nucleic acid amplification and expression profiling</li> <li>Gene expression analysis</li> <li>Genotyping studies</li> <li>Genomics-related applications</li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" style="width: 500px;" border="0"> <tbody> <tr> <td align="center" valign="top"><strong>Number of 20 &micro;l Reactions</strong></td> <td align="center" valign="top"><strong>Supermix Volume, ml</strong></td> <td align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp;&nbsp;200</td> <td style="padding-left: 70px;" valign="top">&nbsp; 2 (2 x 1 ml)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725200-ssofast-evagreen-supermix-200-x-20-ul-rxns-2-ml-2-x-1-ml"><span style="font-size: 11px;">1725200</span></a></td> </tr> <tr> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp;&nbsp;500</td> <td style="padding-left: 70px;" valign="top">&nbsp; 5 (5 x 1 ml)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725201-ssofast-evagreen-supermix-500-x-20-ul-rxns-5-ml-5-x-1-ml"><span style="font-size: 11px;">1725201</span></a></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">1,000</td> <td style="padding-left: 70px;" valign="top">10 (10 x 1 ml)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725202-ssofast-evagreen-supermix-1-000-x-20-ul-rxns-10-ml-10-x-1-ml"><span style="font-size: 11px;">1725202</span></a></td> </tr> <tr> <td style="padding-left: 80px;" valign="top">2,000</td> <td style="padding-left: 70px;" valign="top">&nbsp; 20 (1 x 20 ml)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725203-ssofast-evagreen-supermix-2-000-x-20-ul-rxns-20-ml-1-x-20-ml"><span style="font-size: 11px;">1725203</span></a></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">2,500</td> <td style="padding-left: 70px;" valign="top">25 (5 x 5 ml)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1725204-ssofast-evagreen-supermix-2-500-x-20-ul-rxns-25-ml-5-x-5-ml"><span style="font-size: 11px;">1725204</span></a></td> </tr> <tr> <td style="padding-left: 80px;" valign="top">5,000</td> <td style="padding-left: 70px;" valign="top">&nbsp; 50 (10 x 5 ml)</td> <td style="padding-left: 40px;" valign="top"><span style="font-size: 11px;">1725205</span></td> </tr> </tbody> </table> <br /> <p>See the <a href="/en-us/product/ssofast-evagreen-supermixes">SsoFast EvaGreen Supermix</a> product page for more information and documentation.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>View all <a href="/en-us/category/supermixes-for-pcr-real-time-pcr">Real-Time PCR Supermixes and Kits</a></li> <li><a href="/en-us/product/primepcr-pcr-primers-assays">PrimePCR&trade; PCR Primers &amp; Assays</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/pcr-reagents-qpcr-reagents">PCR and qPCR Reagents</a></li> <li><a href="/en-us/category/real-time-pcr-detection-systems">Real-Time PCR Detection Systems</a></li> <li><a href="/en-us/category/pcr-plastic-consumables">PCR Plastic Consumables</a></li> <li><a href="/en-us/category/pcr-instrument-validation-tools-services">Instrument Validation Tools and Services</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>SsoFast EvaGreen Supermix <a href="/webroot/web/pdf/lsr/literature/Bulletin_10014647.pdf">Product Insert</a></li> <li>Amplification Plastics and Reagents <a href="/webroot/web/pdf/lsr/literature/10022527.pdf">Selection Guide</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/qpcr-real-time-pcr">qPCR/Real-Time PCR</a></li> <li><a href="/en-us/applications-technologies/genomics">Genomics</a></li> </ul> <p class="caption">*U.S. patents 6,627,424; 7,541,170; and 7,560,260</p> /webroot/web/html/lsr/products/SKUs/autopopulated 172-5205.html /webroot/web/html/lsr/products/SKUs/autopopulated/172-5205.html &nbsp; false $3,543.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725205&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725205&CartQuote=QT&quantity= 1442 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725205&AddToDefaultWishList=true&quantity= 41106300 3543.00 1725205
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Number Description Options
10014647 Instruction Manual, SsoFast EvaGreen Supermix, Rev D Click to download
5816 SsoFast™ EvaGreen® Supermix Flier, Rev B Click to download
5936 SsoFast EvaGreen Supermix Stability for Automated qPCR, Rev A Click to download
10018778 SsoFast™ EvaGreen Control Assay Instructions, Rev A Click to download
5924 Stem Cell Basics for Life Science Researchers Brochure, Rev A Click to download [ Add to Cart (Free) ]
6252 Reagent Comparison Guide for Real-Time PCR Brochure, Rev C Click to download [ Add to Cart (Free) ]
7178 Amplification: Real-Time PCR Reagents and Kits Brochure Click to download
6090 Amplification Reagents and Plastics Brochure Click to download [ Add to Cart (Free) ]
6763 Gene Expression Real-Time PCR Workflow Flier Click to download [ Add to Cart (Free) ]

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