iQ Supermix

iQ Supermix

iQ Supermix is specially formulated for real-time PCR and has been tested for reliable amplification over a wide dynamic range of human genomic and plasmid DNA concentrations.

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Overview

NEW PRODUCT

SsoAdvanced Universal Supermixes

Affordable, superior performance alternative to SsoFast Supermixes

  • Guaranteed performance in all conditions
  • Use on any real-time PCR instrument without adjusting ROX concentration
SsoAdvanced Universal Supermixes

iQ Supermix is specially formulated for real-time PCR and has been tested for reliable amplification over a wide dynamic range of human genomic and plasmid DNA concentrations. It contains hot-start iTaq DNA Polymerase, dNTPs qualified for quantitative PCR, and buffer at concentrations optimal for real-time PCR assays. iQ Supermix facilitates reaction optimization and is qualified for use with all Bio-Rad real-time detection systems.

Key Features

  • Increased throughput; only template, primers, and probes are needed
  • Convenient reaction assembly at room temperature
  • Easy-to-use formulation minimizes contamination

Applications and Uses

  • PCR and real-time PCR analysis
  • Gene expression analysis
  • Genotyping studies
  • Genomics-related applications

Related Products

Select the best real-time PCR supermix for your application:

  • Bio-Rad’s Universal Supermixes can be used in all real-time PCR systems — both ROX-dependent and ROX-independent instruments
  • SsoAdvanced Universal Supermixes feature our patented Sso7d fusion polymerase*
Key Features of Bio-Rad Real-Time PCR Supermixes iQ Supermixes SsoAdvanced Universal Supermixes
Sso7d fusion protein technology No Yes
Sensitive detection of low-level targets 3 star 5 star
Efficiency with difficult target sequences 3 star 5 star
Specificity 1 star 5 star
Tolerance to PCR inhibitors 0 star 5 star
Broad range of reaction conditions 1 star 5 star
Fast cycling 1 star 5 star
Instrument compatibility Select instruments Any instrument

 

Related Categories

* U.S. patents 7,560,260.

 

Specifications

Storage at –20°C
Guaranteed for 12 months at –20°C in a constant temperature freezer (multiple freeze thaws not recommended)
Storage at +4°C
Guaranteed for 6 months at 4°C

Ordering

items

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1708860
100 x 50 µl reactions, 2x real-time PCR mix, contains dNTPs, 6 mM MgCl2, 50 U/ml hot-start iTaq™ DNA polymerase
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1708862
500 x 50 µl reactions, 2x real-time PCR mix, contains dNTPs, 6 mM MgCl2, 50 U/ml hot-start iTaq™ DNA polymerase
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1708864
1,000 x 50 µl reactions, real-time PCR mix, 2x mix contains dNTPs, 50 U/ml iTaq™ DNA polymerase, 6 mM MgCl2
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1708865
2,000 x 50 µl reactions, real-time PCR mix, 2x mix contains dNTPs, 50 U/ml iTaq™ DNA polymerase, 6 mM MgCl2

Documents

Number Description Options
4106204
Instruction Manual, iQ Supermix PCR Reagents, Rev D
2764
iQ Supermixes for qPCR Flier, Rev E
6252
Reagent Comparison Guide for Real-Time PCR Brochure, Rev C
[ Add to Cart (Free) ]
7178
Amplification: Real-Time PCR Reagents and Kits Brochure
[ Add to Cart (Free) ]
6090
Amplification Reagents and Plastics Brochure
6763
Gene Expression Real-Time PCR Workflow Flier
[ Add to Cart (Free) ]