Immun-Blot Low Fluorescence PVDF Membrane

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Low Fluorescence PVDF Membrane

Overview

Bio-Rad Protein Transfer Membrane Offerings
Protein Membranes

Bio-Rad's PVDF membranes are available in rolls, precut sheets, or convenient membrane/filter paper sandwiches.

Immun-Blot® PVDF Membrane
  • Ideal for chemiluminescent and colorimetric western blots
  • Retains target protein very strongly during protein transfer but also reduces nonspecific protein binding
Immun-Blot® Low Fluorescence PVDF Membrane
  • Same benefits as standard PVDF membranes plus minimal autofluorescence and high sensitivity
  • Best choice for multiplex fluorescent immunoblotting
Sequi-Blot™ PVDF Membrane
  • Ideal for protein sequencing: retains target protein very strongly during protein transfer
  • Provides the binding capacity to sequence even low-abundance samples
Nitrocellulose Membrane, 0.45 µm
  • Proven medium for western, northern, and Southern blotting
  • 45 µm pore size is recommended for most analytical blotting, including protein, ssDNA, and RNA transfers
  • 0.2 µm pore size is optimized for transferring nucleic acids and low molecular–weight proteins
Nitrocellulose Membrane, 0.2 µm
Supported Nitrocellulose Membrane, 0.45 µm
  • Dense 100% nitrocellulose cast on both sides of an inert polyester support
  • Easy to handle, wets readily, and does not have the brittleness often associated with unsupported nitrocellulose
Supported Nitrocellulose Membrane, 0.2 µm
LF PVDF Membranes Have Lower Autofluorescence than PVDF Membranes


LF PVDF membranes have lower autofluorescence than traditional PVDF membranes. Membranes were scanned dry in blue excitation for 5 sec, green excitation for 5 sec, and red excitation for 20 sec. Membrane fluorescence was determined using Image Lab™ Software.

Linear Signal for Accurate Blot Quantitation
Transferrin in albumin depleted serum

Linear signal for accurate blot quantitation. Different dilutions of an albumin-depleted human serum sample were loaded on a Criterion™ TGX™ 4–20% Gel and transferred to a low fluorescence PVDF membrane using tank blotting. The primary antibody used was rabbit anti-transferrin antibody and the secondary antibody was donkey anti-rabbit Alexa Fluor 568. Quantitation of the band was performed using Image Lab™ Software.

LF PVDF Membranes Are Compatible with Bio-Rad Imaging Systems
ChemiDoc MP System Gel Doc XR+System ChemiDoc MP
ChemiDoc™ MP System Gel Doc™ XR+ System ChemiDoc Touch System
Gel Doc EZ System PharosFX Plus System ChemiDoc XRS System
Gel Doc EZ System PharosFX™ Plus System ChemiDoc XRS+ System

Use LF PVDF membranes on any of the above Bio-Rad imaging systems.

Multiplex Detection of Two Targets Simultaneously


Multiplex detection of two targets simultaneously. Albumin-depleted human serum samples spiked with STI and Profinia™ GST extract run on a Criterion™ TGX™ 4–20% Stain-Free Gel, transferred to a membrane of choice, and blotted. The primary antibodies used were goat anti-GST and rabbit anti-STI. The secondary antibodies used were anti-rabbit Alexa Fluor 546 and anti-goat Alexa Fluor 488.

Multiple Packaging Options for Your Needs

LF PVDF membranes are available precut for mini or midi gel sizes in 10- or 20-pack configurations, or as a large roll.

Blotting Membrane and Filter Paper Selection Guide
Membrane Binding Capacity
(µg/cm2)
Applications
Nitrocellulose 80–100 Immunoblotting
Supported nitrocellulose 80–100 Immunoblotting
Immun-Blot® PVDF 150–160 Immunoblotting
Immun-Blot® low fluorescence PVDF 150–160 Fluorescent immunoblotting
Sequi-Blot™ PVDF 170–200 Protein sequencing
Zeta-Probe® 150 DNA/RNA hybridization
Zeta-Probe GT 150 DNA/RNA hybridization
C/P Lift 150 Colony/plaque screening

Blotting Apparatus Precut Membrane Sizes Precut Filter Paper Sizes Membrane/Filter Paper Sandwiches
Mini Trans-Blot® cell 7 x 8.4 cm 7.5 x 10.5 cm 7 x 8.5 cm
Criterion™ blotter 8.5 x 13.5 cm 9.5 x 15.2 cm 8.5 x 13.5 cm
Trans-Blot® cell 13.5 x 16.5 cm 15 x 20 cm  
Trans-Blot® Plus cell 25 x 28 cm 26.5 x 28  
  26.5 x 28 cm 26.5 x 28 cm  
Trans-Blot® SD cell 7 x 8.5 cm 14 x 16 cm 7 x 8.5 cm
  11.5 x 16 cm 7 x 8.4 cm 8.5 x 13.5 cm
  15 x 15 cm 8 x 13.5 cm  
  15 x 9.2 cm 18 x 18.5 cm  
  20 x 20 cm    
Mini-PROTEAN® II
multiscreen apparatus
7 x 8.4 cm 7 x 8.4 cm 7 x 8.5 cm
  7 x 8.5 cm    
Bio-Dot® apparatus 9 x 12 cm    
Bio-Dot® SF apparatus 9 x 12 cm 7.7 x 11.3 cm  

For more information, download bulletins 1939 and 2212.

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Immun-Blot Low Fluorescence PVDF Membrane offers the same benefits as standard PVDF membranes in addition to minimal autofluorescence and high sensitivity, making it the premium choice for multiplex fluorescent immunoblotting needs.

Features and Benefits

  • Low autofluorescence allows long exposure times and maximizes the sensitivity of fluorescent blot imaging systems
  • High protein retention and broad dynamic range result in more accurate protein quantitation from blot
  • Compatible with most multiplex fluorescent blotting protocols for detection of two or more proteins on the same blot
  • Available in two formats:
    • Convenient precut membrane and filter paper sets
    • Economical rolls for manual cutting and conversion

Applications

  • Fluorescent blotting using RGB fluorophores
  • Compatible with chemiluminescent and colorimetric detection methods

Please refer to the Immun-Blot Low Fluorescence PVDF brochure for more information and data.

See the Blotting Membrane and Filter Paper Selection Guide for more information on the different membrane types and size selections by gel type.

 

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Immun-Blot® Low Fluorescence PVDF/Filter Paper Sets

1620260
Pkg of 10, 0.45 µm, 7 x 8.5 cm, precut low fluorescence PVDF/filter paper for immunoblotting

1620260 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/162-0260_1308788358.xml 1620260 /default/main/bio-rad/WORKAREA/default 06/22/11 05:12 PM en_US jdl0t1ue 2017-11-14 07:08:24 true true internet/product_SKU jdl0t1ue templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/162-0260_1308788358.xml LSR 2019-05-04 19:51:19 /default/main/bio-rad/STAGING N /default/main/bio-rad en 06/22/21 05:12 PM 1556001557199 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 19:51:19 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/162-0260_1308788358.xml templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/162-0260_1308788358.xml 1620260-immun-blot-low-fluorescence-pvdf-filter-paper-sets 1620260 Immun-Blot Low Fluorescence PVDF Filter Paper Set, Mini Size, 10 Pack Immun-Blot<sup>&reg;</sup> Low Fluorescence PVDF/Filter Paper Sets Pkg of 10, 0.45 µm, 7 x 8.5 cm, precut low fluorescence PVDF/filter paper for immunoblotting Pkg of 10, 0.45 µm, 7 x 8.5 cm, precut low fluorescence PVDF/filter paper for immunoblotting /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/162-0260_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/162-0260_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/162-0260_feature.jpg ,VN Karen Moss 162-0260 Pkg of 10, 7 x 8.5 cm, precut low fluorescence PVDF/filter paper for immunoblotting 1620260, immun-blot, low, fluorescence, pvdf/filter, paper, sets, pvdf, filter, immunoblot, immunblot, immunoblotting, 0.45um, precut, preassembled, blotting, membrane, western, blot, blots, westerns, long, exposure 06/22/11 05:12 PM 06/22/21 05:12 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <p>This Immun-Blot<sup>&reg;</sup> LF PVDF membrane package contains 10 sheets of 7 x 8.5 cm precut membrane and 20 sheets of precut filter paper. The pore size is 0.45 &micro;m and binding capacity is 150&ndash;160 &micro;g/cm<sup>2</sup>.</p> <p>Immun-Blot LF PVDF membrane is ideal for fluorescent blotting applications because of its low autofluorescence and high protein retention properties, allowing for long exposure times without raising background and maximizing the sensitivity of the fluorescent blot imaging system. The precut and preassembled blotting membrane and filter paper sets are designed to simplify western blotting setup.</p> /webroot/web/html/lsr/products/electrophoresis/sku_view/description/global 162-0260_desc.html /webroot/web/html/lsr/products/electrophoresis/sku_view/description/global/162-0260_desc.html Material type 1800 Low fluorescence PVDF 1 0 Pore size, &micro;m 1991 0.45 1 0 Format 1660 Precut membrane and filter paper 1 0 Dimensions, cm 1570 7 x 8.5 1 0 false $122.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1620260&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1620260&CartQuote=QT&quantity= 1199 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1620260&AddToDefaultWishList=true&quantity= 41105300 122.00 1620260
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Immun-Blot® Low Fluorescence PVDF/Filter Paper Sets

1620261
Pkg of 20, 0.45 µm, 7 x 8.5 cm, precut low fluorescence PVDF/filter paper for immunoblotting

1620261 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/162-0261_1308788556.xml 1620261 /default/main/bio-rad/WORKAREA/default 06/22/11 05:20 PM en_US jdl0t1tu 2017-11-14 07:08:24 true true internet/product_SKU jdl0t1tu templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/162-0261_1308788556.xml LSR 2019-05-04 19:51:15 /default/main/bio-rad/STAGING N /default/main/bio-rad en 06/22/21 05:20 PM 1556001557241 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 19:51:15 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/162-0261_1308788556.xml templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/162-0261_1308788556.xml 1620261-immun-blot-low-fluorescence-pvdf-filter-paper-sets 1620261 Immun-Blot Low Fluorescence PVDF Filter Paper Set, Mini Size, 20 Pack Immun-Blot<sup>&reg;</sup> Low Fluorescence PVDF/Filter Paper Sets Pkg of 20, 0.45 µm, 7 x 8.5 cm, precut low fluorescence PVDF/filter paper for immunoblotting Pkg of 20, 0.45 µm, 7 x 8.5 cm, precut low fluorescence PVDF/filter paper for immunoblotting /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/162-0261_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/162-0261_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/162-0261_feature.jpg ,VN Karen Moss 162-0261 Pkg of 20, 7 x 8.5 cm, precut low fluorescence PVDF/filter paper for immunoblotting 1620261, immun-blot, low, fluorescence, pvdf/filter, paper, sets, pvdf, filter, immunoblot, immunblot, immunoblotting, 0.45um, precut, preassembled, blotting, membrane, western, blot, blots, westerns, long, exposure 06/22/11 05:20 PM 06/22/21 05:20 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <p>This Immun-Blot<sup>&reg;</sup> LF PVDF membrane package contains 20 sheets of 7 x 8.5 cm precut membrane and 40 sheets of precut filter paper. The pore size is 0.45 &micro;m and binding capacity is 150&ndash;160 &micro;g/cm<sup>2</sup>.</p> <p>Immun-Blot LF PVDF membrane is ideal for fluorescent blotting applications because of its low autofluorescence and high protein retention properties, allowing for long exposure times without raising background and maximizing the sensitivity of the fluorescent blot imaging system. The precut and preassembled blotting membrane and filter paper sets are designed to simplify western blotting setup.</p> /webroot/web/html/lsr/products/electrophoresis/sku_view/description/global 162-0261_desc.html /webroot/web/html/lsr/products/electrophoresis/sku_view/description/global/162-0261_desc.html Material type 1800 Low fluorescence PVDF 1 0 Pore size, &micro;m 1991 0.45 1 0 Format 1660 Precut membrane and filter paper 1 0 Dimensions, cm 1570 7 x 8.5 1 0 false $239.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1620261&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1620261&CartQuote=QT&quantity= 1199 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1620261&AddToDefaultWishList=true&quantity= 41105300 239.00 1620261
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Immun-Blot® Low Fluorescence PVDF/Filter Paper Sets

1620262
Pkg of 10, 0.45 µm, 8.5 x 13.5 cm, precut low fluorescence PVDF/filter paper for immunoblotting

1620262 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/162-0262_1308788706.xml 1620262 /default/main/bio-rad/WORKAREA/default 06/22/11 05:22 PM en_US jdl0t1uk 2017-11-14 07:08:24 true true internet/product_SKU jdl0t1uk templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/162-0262_1308788706.xml LSR 2019-05-04 19:51:21 /default/main/bio-rad/STAGING N /default/main/bio-rad en 06/22/21 05:22 PM 1556001557284 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 19:51:21 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/162-0262_1308788706.xml templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/162-0262_1308788706.xml 1620262-immun-blot-low-fluorescence-pvdf-filter-paper-sets 1620262 Immun-Blot Low Fluorescence PVDF Filter Paper Set, Midi Size, 10 Pack Immun-Blot<sup>&reg;</sup> Low Fluorescence PVDF/Filter Paper Sets Pkg of 10, 0.45 µm, 8.5 x 13.5 cm, precut low fluorescence PVDF/filter paper for immunoblotting Pkg of 10, 0.45 µm, 8.5 x 13.5 cm, precut low fluorescence PVDF/filter paper for immunoblotting /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/162-0262_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/162-0262_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/162-0262_feature.jpg ,VN Karen Moss 162-0262 Pkg of 10, 8.5 x 13.5 cm, precut low fluorescence PVDF/filter paper for immunoblotting 1620262, immun-blot, low, fluorescence, pvdf/filter, paper, sets, pvdf, filter, immunoblot, immunblot, immunoblotting, 0.45um, precut, preassembled, blotting, membrane, western, blot, blots, westerns, long, exposure 06/22/11 05:22 PM 06/22/21 05:22 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <p>This Immun-Blot<sup>&reg;</sup> LF PVDF membrane package contains 10 sheets of 8.5 x 13.5 cm precut membrane and 20 sheets of precut filter paper. The pore size is 0.45 &micro;m and binding capacity is 150&ndash;160 &micro;g/cm<sup>2</sup>.</p> <p>Immun-Blot LF PVDF membrane is ideal for fluorescent blotting applications because of its low autofluorescence and high protein retention properties, allowing for long exposure times without raising background and maximizing the sensitivity of the fluorescent blot imaging system. The precut and preassembled blotting membrane and filter paper sets are designed to simplify western blotting setup.</p> /webroot/web/html/lsr/products/electrophoresis/sku_view/description/global 162-0262_desc.html /webroot/web/html/lsr/products/electrophoresis/sku_view/description/global/162-0262_desc.html Material type 1800 Low fluorescence PVDF 1 0 Pore size, &micro;m 1991 0.45 1 0 Format 1660 Precut membrane and filter paper 1 0 Dimensions, cm 1570 8.5 x 13.5 1 0 false $185.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1620262&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1620262&CartQuote=QT&quantity= 1199 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1620262&AddToDefaultWishList=true&quantity= 41105300 185.00 1620262
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$185.00 
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Immun-Blot® Low Fluorescence PVDF/Filter Paper Sets

1620263
Pkg of 20, 0.45 µm, 8.5 x 13.5 cm, precut low fluorescence PVDF/filter paper for immunoblotting

1620263 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/162-0263_1308788820.xml 1620263 /default/main/bio-rad/WORKAREA/default 06/22/11 05:25 PM en_US jdl0t1uy 2017-11-14 07:08:24 true true internet/product_SKU jdl0t1uy templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/162-0263_1308788820.xml LSR 2019-05-04 19:51:13 /default/main/bio-rad/STAGING N /default/main/bio-rad en 06/22/21 05:25 PM 1556001557326 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 19:51:13 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/162-0263_1308788820.xml templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/162-0263_1308788820.xml 1620263-immun-blot-low-fluorescence-pvdf-filter-paper-sets 1620263 Immun-Blot Low Fluorescence PVDF Filter Paper Set, Midi Size, 20 Pack Immun-Blot<sup>&reg;</sup> Low Fluorescence PVDF/Filter Paper Sets Pkg of 20, 0.45 µm, 8.5 x 13.5 cm, precut low fluorescence PVDF/filter paper for immunoblotting Pkg of 20, 0.45 µm, 8.5 x 13.5 cm, precut low fluorescence PVDF/filter paper for immunoblotting /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/162-0263_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/162-0263_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/162-0263_feature.jpg ,VN Karen Moss 162-0263 Pkg of 20, 8.5 x 13.5 cm, precut low fluorescence PVDF/filter paper for immunoblotting 1620263, immun-blot, low, fluorescence, pvdf/filter, paper, sets, pvdf, filter, immunoblot, immunblot, immunoblotting, 0.45um, precut, preassembled, blotting, membrane, western, blot, blots, westerns, long, exposure 06/22/11 05:25 PM 06/22/21 05:25 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <p>This Immun-Blot<sup>&reg;</sup> LF PVDF membrane package contains 20 sheets of 8.5 x 13.5 cm precut membrane and 40 sheets of precut filter paper. The pore size is 0.45 &micro;m and binding capacity is 150&ndash;160 &micro;g/cm<sup>2</sup>.</p> <p>Immun-Blot LF PVDF membrane is ideal for fluorescent blotting applications because of its low autofluorescence and high protein retention properties, allowing for long exposure times without raising background and maximizing the sensitivity of the fluorescent blot imaging system. The precut and preassembled blotting membrane and filter paper sets are designed to simplify western blotting setup.</p> /webroot/web/html/lsr/products/electrophoresis/sku_view/description/global 162-0263_desc.html /webroot/web/html/lsr/products/electrophoresis/sku_view/description/global/162-0263_desc.html Material type 1800 Low fluorescence PVDF 1 0 Pore size, &micro;m 1991 0.45 1 0 Format 1660 Precut membrane and filter paper 1 0 Dimensions, cm 1570 8.5 x 13.5 1 0 false $361.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1620263&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1620263&CartQuote=QT&quantity= 1199 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1620263&AddToDefaultWishList=true&quantity= 41105300 361.00 1620263
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Immun-Blot® Low Fluorescence PVDF membrane

1620264
Pkg of 1 roll, 0.45 µm, 28 cm x 3.8 m, low fluorescence PVDF membrane for immunoblotting

1620264 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/162-0264_1308788937.xml 1620264 /default/main/bio-rad/WORKAREA/default 06/22/11 05:27 PM en_US jdl0t1u0 2017-11-14 07:08:24 true true internet/product_SKU jdl0t1u0 templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/162-0264_1308788937.xml LSR 2019-05-04 19:51:00 /default/main/bio-rad/STAGING N /default/main/bio-rad en 06/22/21 05:27 PM 1556001557368 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 19:51:00 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/162-0264_1308788937.xml templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/162-0264_1308788937.xml 1620264-immun-blot-low-fluorescence-pvdf-membrane 1620264 Immun-Blot Low Fluorescence PVDF Roll Immun-Blot<sup>&reg;</sup> Low Fluorescence PVDF membrane Pkg of 1 roll, 0.45 µm, 28 cm x 3.8 m, low fluorescence PVDF membrane for immunoblotting Pkg of 1 roll, 0.45 µm, 26.5 cm x 3.8 m, low fluorescence PVDF membrane for immunoblotting /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/162-0177_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/162-0177_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/162-0177_feature.jpg ,VN Karen Moss 162-0264 Pkg of 1 roll, 26.5 cm x 3.8 m, low fluorescence PVDF membrane for immunoblotting 1620264, immun-blot, low, fluorescence, pvdf, membrane, immunblot, immunoblot, immunoblotting, western, blotting, blot, blots, transfer, binding, 0.45um, roll, trans-blot, long, exposure 06/22/11 05:27 PM 06/22/21 05:27 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <p>This Immun-Blot<sup>&reg;</sup> LF PVDF membrane is packaged as a 28 cm wide, 3.8 m long bulk roll in an easy-to-access box. The pore size is 0.45 &micro;m and binding capacity is 150&ndash;160 &micro;g/cm<sup>2</sup>.</p> <p>Immun-Blot LF PVDF membrane is ideal for fluorescent blotting applications because of its low autofluorescence and high protein retention properties, allowing for long exposure times without raising background and maximizing the sensitivity of the fluorescent blot imaging system.</p> /webroot/web/html/lsr/products/electrophoresis/sku_view/description/global 162-0264_desc.html /webroot/web/html/lsr/products/electrophoresis/sku_view/description/global/162-0264_desc.html Material type 1800 Low fluorescence PVDF 1 0 Pore size, &micro;m 1991 0.45 1 0 Format 1660 Roll 1 0 Dimensions, cm 1570 28 x 380 1 0 false $706.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1620264&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1620264&CartQuote=QT&quantity= 1199 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1620264&AddToDefaultWishList=true&quantity= 41105300 706.00 1620264
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Number Description Options
6570 Experimental Protocol for Multiplex Fluorescent Blotting Using the ChemiDoc™ MP Imaging System, Rev A Click to download
2895 Protein Blotting Guide, Ver C Click to download
6116 Immun-Blot® LF PVDF Membranes Product Information Sheet, Rev B Click to download
6166 Multiplex Fluorescent Blot Detection: A Troubleshooting Guide, Rev A Click to download
6148 Transfer of High Molecular Weight Proteins to Membranes: A Comparison of Transfer Efficiency Between Blotting Systems, Rev B Click to download

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