Immun-Blot Low Fluorescence PVDF Membrane

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Overview

Bio-Rad’s low-fluorescence PVDF membrane is validated for fluorescent western blotting applications. The low autofluorescence of this membrane allows for longer exposure and higher sensitivity without increased background.
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Description

Description

Immun-Blot Low Fluorescence PVDF Membrane offers the same benefits as standard PVDF membranes in addition to minimal autofluorescence and high sensitivity, making it the premium choice for multiplex fluorescent immunoblotting needs.

Features and Benefits

  • Low autofluorescence allows long exposure times and maximizes the sensitivity of fluorescent blot imaging systems
  • High protein retention and broad dynamic range result in more accurate protein quantitation from blot
  • Compatible with most multiplex fluorescent blotting protocols for detection of two or more proteins on the same blot
  • Available in two formats:
    • Convenient precut membrane and filter paper sets
    • Economical rolls for manual cutting and conversion

Applications

  • Fluorescent blotting using RGB fluorophores
  • Compatible with chemiluminescent and colorimetric detection methods

Please refer to the Immun-Blot Low Fluorescence PVDF brochure for more information and data.

See the Blotting Membrane and Filter Paper Selection Guide for more information on the different membrane types and size selections by gel type.

 

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1620260
Pkg of 10, 0.45 µm, 7 x 8.5 cm, precut low fluorescence PVDF/filter paper for immunoblotting
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1620261
Pkg of 20, 0.45 µm, 7 x 8.5 cm, precut low fluorescence PVDF/filter paper for immunoblotting

Documents

Documents

Number Description Options
6570
Experimental Protocol for Multiplex Fluorescent Blotting Using the ChemiDoc™ MP Imaging System, Rev A
2895
Protein Blotting Guide, Ver C
6116
Immun-Blot® LF PVDF Membranes Product Information Sheet, Rev B
6166
Multiplex Fluorescent Blot Detection: A Troubleshooting Guide
6148
Transfer of High Molecular Weight Proteins to Membranes: A Comparison of Transfer Efficiency Between Blotting Systems, Rev B