Discontinued Product
The ChemiDoc XRS+ System has been discontinued. As an alternative, view the ChemiDoc Imaging System.
Where possible, Bio-Rad will continue to provide ChemiDoc XRS+ customers with support through 2028.
Find the right products for you using the free Western Blot Selector Tool
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Bio-Rad's fluorescent western blotting workflow is a seamless integration of products designed to work together to offer guaranteed results. This validated set of solutions will make it easy for you to get better data every time.
ChemiDoc MP Imaging System: Imagine No Compromises
With the ChemiDoc MP System, the superior quantitation of digital meets the sensitivity of film and delivers the best fluorescence performance with the brightest new fluorophore.
Move Beyond Traditional Imaging
Traditional laser scanning systems and X-ray film are a thing of the past. Get higher multiplexing capabilities without sacrificing the performance you expect from a best-in-class imaging system.
More Flexibility in Dye Selection
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- DyLight 488
- Alexa Fluor 488
- Cy2
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- StarBright B520
- DyLight 549
- Alexa Fluor 546
- Cy3
- hFAB
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- DyLight 650
- Alexa Fluor 647
- Cy5
- SYPRO Ruby
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- StarBright B700
- DyLight 680
- Alexa Fluor 680
- Cy5.5
- IRDye 680
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- DyLight 800
- Alexa Fluor 790
- Cy7
- IRDye 800
10 Tips for Western Blot Detection of Phosphorylation Events
ChemiDoc Imaging Systems
Complete Capabilities: Superior Images
Better Options
With the ChemiDoc MP, one imaging system covers all of your gel documentation, chemiluminescence, and multiplex fluorescence needs.
Stain-Free
Total Protein Normalization
Learn how to properly design a quantitative western blotting experiment and faithfully compare protein expression levels, without concern for changing or overexpressed HKP’s.
See Data »
Chemiluminescence
Replaces Film
Save money on consumables and reduce environmental waste without compromising on performance. Get the sensitivity of film and the convenience of digital documentation.
See Data »
Multiplex Fluorescence
3 Colors
Save sample, avoid the errors commonly associated with stripping, reprobing, and cutting blots and answer more complex biological questions by optimizing your experiments to fluorescence.
See Data »
Better Data
See up to three signals at once or look at a subset of signals. Plus, you can normalize beyond a single protein with stain-free total protein normalization.
Explore blot combinations by making a selection on the right.
Better Experience
Image Lab Touch Software
Image Lab Touch Software works with the ChemiDoc MP Imaging System and provides easy and intuitive onboard acquisition software. Built for labs with multiple users, Image Lab Touch Software teaches as you tap and explore, selects optimal light source, and permits both manual entry and automated calculation of exposure times.
Intuitive Imaging at Your Fingertips
Get instructions showing how to capture images on the ChemiDoc MP System via Image Lab Touch Software
» View tutorials
Stain-Free Western Blotting
Stain-free western blotting allows you to quickly check electrophoresis and blot transfer quality and obtain truly quantitative western blotting results, updating traditional blotting techniques with innovative tools.
Faster Results. Better Data.
Antibodies for Fluorescent Western Blotting
Primary Antibodies
New Phospho-Specific PrecisionAb Antibodies
- Highly specific antibodies to detect phosphorylation events
- Validated by comparing phosphorylation levels in both treated versus untreated cell lysates and by dephosphorylating western blot membranes
- Showcased against total protein antibodies
PrecisionAb Antibodies
- Highly specific and sensitive
- Validated for western blotting applications
- Each antibody is screened against up to 12 whole-cell lysates expressing endogenous protein levels
hFAB Rhodamine Housekeeping Protein Fluorescent Primary Antibodies
- One-step, directly conjugated, fluorescence detection solution for detecting commonly used HKP's (actin, tubulin, and GAPDH)
- Eliminates cross-reactivity with primary and secondary antibodies from other species
- HuCAL® technology allows you to choose any appropriate host species for your other antibodies
Secondary Antibodies
StarBright Blue 700 Fluorescent Secondary Antibodies
- Best choice for maximum sensitivity
- Novel polymer-based chemistry is equivalent to femto ECL
- Superior signal-to-noise ratio
StarBright Blue 520 Fluorescent Secondary Antibodies
- Novel polymer-based chemistry is equivalent to femto ECL
- Ideal for multiplexing
DyLight Fluorescent Secondary Antibodies
- Traditional fluorescent solution
- Available in many colors and host species
- Ideal for routine fluorescent detection
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Western Blot University
Courses designed to make you a western blotting expert.
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Introducing the Western Blot Learning Center
Perfect your western blotting. Learn from the experts.
Free 26-Lane Electrophoresis & Western Blotting Layout Post-It Pad
Plan, track, and never miss another step.
Request Yours NowBlotting Membrane
Immun-Blot Low Fluorescence PVDF Membrane
- Minimal autofluorescence allows long exposure times and maximizes the sensitivity of digital imaging systems
- High protein retention and broad dynamic range enables more accurate quantitation
- Compatible with most multiplex fluorescent blotting protocols
Pick the Best Standards for Your Blots!
Just click on the light source in our interactive guide and see how banding pattern changes with light source. This interactive guide helps you pick the best protein standard for your gels and blots.
Benefits of Fluorescent Western Blotting
Learn more about the benefits of Fluorescent Western Blotting by checking out our video and document resources.
Light Up Your Lab
Get a free excitation/emission reference poster to make it easier to select the right fluorophore for your next experiment.
Request a Free Fluorophore Poster
Documents
The Complete Guide to Stain-Free Western Blotting
(PDF 2.1 MB)Learn about the benefits of the proprietary Stain-Free technology from Bio-Rad Laboratories and get guidance for each step of the workflow.
Stain-Free Western Blotting Brochure
(PDF 593 KB)Compare Stain-Free Western Blotting with traditional Western Blotting Workflows in this brochure.
Stain-Free Western Blotting Publication List
(PDF 118 KB)See how others are using Stain-Free Western Blotting to advance their research.
Western Blotting Protocol Library
Filter by your laboratory set-up and reagents to get a custom western blotting protocol that best fits your needs.
Stain-Free Western Blotting Protocol
(PDF 358 KB)Get faster, more quantitative, and more reliable results with our Stain-Free Western Blotting protocol.
Stain-Free Approach to Western Blotting
(PDF 864 KB)Use stain-free technology for total protein normalization as an alternative to the standard blot normalization process.
ChemiDocMP Brochure
(PDF 1.26 MB)Explore the features and benefits of the new high-end imaging system for the best fluorescence and chemiluminescence detection.
Summary of Features and Benefits
(PDF 242 KB)See a summary of the features and benefits of the new ChemiDoc MP high-end imaging system.
StarBright Secondary Antibody Brochure
(PDF 246 KB)Learn all about StarBright Secondary Antibodies.
StarBright Secondary Antibody Protocol
(PDF 255 KB)Find a detailed protocol with tips and tricks to help you get better data with fluorescent western blotting.
Related Products
Western Blotting
Western blotting products include the Stain-Free Western Blotting Workflow, protein transfer systems, blotting membranes, filter paper, premixed blotting buffers and reagents, plus detection kits.
Antibodies
Find solutions for all your antibody needs: an extensive inventory of primary and secondary antibodies, controls, and reagents plus custom products including monoclonal generation in just 8 weeks.