Premixed Transfer Buffers

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Premixed Transfer Buffers

Overview

Transfer Buffer Guide
  1x Formulation Applications
Transfer Buffers*
10x Tris/glycine 25 mM Tris, 192 mM glycine, pH 8.3 Western blotting
10x Tris/CAPS Anode buffer; 60 mM Tris,
40 mM CAPS, 15% methanol, pH 9.6
A discontinuous buffer system that increases transfer efficiency in semi-dry applications
  Cathode buffer, 60 mM Tris,
40 mM CAPS, 0.1% (w/v) SDS, pH 9.6
 
20x SSC 150 mM sodium chloride,
15 mM sodium citrate, pH 7.0
Capillary transfer of agarose gels
Processing Buffers
10x PBS 10 mM sodium phosphate,
150 mM NaCl, pH 7.4
Western blotting wash solution
10x TBS 20 mM Tris,
500 mM NaCl, 1% (w/v) casein, pH 7.4
Western blotting wash solution
1x PBS with 1% casein 10 mM sodium phosphate,
150 mM NaCl, 1% (w/v) casein, pH 7.4
Western blotting blocking buffer (casein blockers recommended for all applications, including those with biotin-avidin complexes)
1x TBS with 1% casein 20 mM Tris, 200 mM NaCl, containing 1% (w/v) casein, pH 7.4 Western blotting blocking buffer (casein blockers recommended for all applications, including those with biotin-avidin complexes)
20x SSC 150 mM sodium chloride,
15 mM sodium citrate, pH 7.0
Northern and Southern blotting prehybridization and hybridization solutions
*These buffers can be used for all gel types and formulations.
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Western blotting transfer buffers must allow effective elution of proteins from the gel matrix as well as their effective binding to membranes. Three premixed transfer buffers are available: 10x Tris/glycine, 10x Tris/CAPS, and 20x SSC.

Processing blots for protein detection is very simple with premixed wash buffers and blocking solutions. Premixed wash buffers in PBS, TBS, and SSC reduce the number of stock solutions to prepare. Premixed blocking buffers, available as 1x PBS with casein and 1x TBS with casein, take the time and effort out of solubilizing casein.

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10x Tris/Glycine Buffer for Western Blots and Native Gels

1610734
Pkg of 1, 1 L, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water

0 1610734 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0734_0921120339825.xml 1610734 09/21/09 12:03 PM en_US true internet/product_SKU 2019-05-04 18:50:16 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0734_0921120339825.xml /default/main/bio-rad/WORKAREA/default jcc1tp27 2017-11-14 07:08:21 true jcc1tp27 templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0734_0921120339825.xml LSR N /default/main/bio-rad en 12/31/37 11:59 PM 1556908421962 2019-05-04 18:50:16 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0734_0921120339825.xml 1610734-10x-tris-glycine-buffer-for-western-blots-native-gels 1 1610734 10x Tris/Glycine 10x Tris/Glycine Buffer for Western Blots and Native Gels Pkg of 1, 1 L, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water 1 L, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, pH 8.3 Pkg of 1, 1 L, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/161-0734_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/161-0734_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/161-0734_feature.jpg 10x Tris/Glycine Buffer for Western Blots and Native Gels ,VN RepEx 161-0734 1 L, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, pH 8.3 161-0734, 1610734, 10x, tris/glycine, native, running, buffer, tris, glycine, polyacrylamide, page, protein, gel, electrophoresis, buffers, reagent, reagents 09/21/09 12:03 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <p>Use 10x Tris/Glycine Buffer as a transfer buffer for western blots or as a running buffer for native protein gel electrophoresis.</p> <ul> <li>For tank or semi-dry blotting for SDS PAGE gels, usually with the addition of 20% methanol</li> <li>For tank blotting of native gels, without methanol</li> <li>As a running buffer for native gels</li> </ul> <h2 class="descHeader">Precast gels that can be used for native electrophoresis</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td style="text-align: left;" width="87" valign="top"><strong>Precast Gel Type</strong></td> <td style="text-align: center;" width="87" valign="top"><strong>Size</strong></td> <td style="text-align: left;" width="87" valign="top"><strong>Gel Chemistry</strong></td> </tr> <tr class="pd_colorbackground"> <td style="text-align: left;" valign="top"><a href="/en-us/product/mini-protean-precast-gels/mini-protean-tgx-precast-gels">Mini-PROTEAN<sup>&reg;</sup> TGX&trade;</a></td> <td style="text-align: center;" valign="top">Mini</td> <td style="text-align: left;" rowspan="2" valign="top">Laemmli-like, long shelf life, fast separation with high resolution</td> </tr> <tr class="pd_colorbackground"> <td style="text-align: left;" valign="top"><a href="/en-us/product/protein-gels/criterion-precast-gels/criterion-tgx-precast-gels">Criterion&trade; TGX&trade;</a></td> <td style="text-align: center;" valign="top">Midi</td> </tr> <tr> <td style="text-align: left;" valign="top"><a href="/en-us/product/mini-protean-precast-gels/mini-protean-tgx-stain-free-precast-gels">Mini-PROTEAN<sup>&reg;</sup> TGX Stain-Free&trade;</a></td> <td style="text-align: center;" valign="top">Mini</td> <td style="text-align: left;" rowspan="2" valign="top">Laemmli-like, long shelf life, fast separation with high resolution, unique trihalo compounds for rapid fluorescent protein detection</td> </tr> <tr> <td style="text-align: left;" valign="top"><a href="/en-us/product/criterion-precast-gels/criterion-tgx-stain-free-precast-gels">Criterion&trade; TGX Stain-Free&trade;</a></td> <td style="text-align: center;" valign="top">Midi</td> </tr> <tr class="pd_colorbackground"> <td style="text-align: left;" valign="top"><a href="/en-us/product/criterion-tris-hcl-precast-gels">Criterion&trade; Tris-HCl</a></td> <td style="text-align: center;" valign="top">Midi</td> <td style="text-align: left;" valign="top">Standard Laemmli</td> </tr> <tr> <td style="text-align: left;" valign="top"><a href="/en-us/product/criterion-precast-gels/criterion-stain-free-tris-hcl-gels">Criterion Stain Free&trade; Tris-HCl</a></td> <td style="text-align: center;" valign="top">Midi</td> <td style="text-align: left;" valign="top">Standard Laemmli, unique trihalo compounds for rapid fluorescent protein detection</td> </tr> <tr class="pd_colorbackground"> <td style="text-align: left;" valign="top"><a href="/en-us/product/criterion-precast-gels/criterion-xt-tris-acetate-precast-gels">Criterion XT Tris-Acetate</a></td> <td style="text-align: center;" valign="top">Midi</td> <td style="text-align: left;" valign="top">Discontinuous buffer ion fronts form moving boundaries to stack, and then separate proteins</td> </tr> </tbody> </table> <p class="caption" style="margin-top: -10px;">Note: Most proteins have an acidic or slightly basic pI (~3&ndash;8) and are run with the power supply connected to the electrophoresis chamber as for SDS-PAGE. If more basic proteins (pl &gt;8.5) of interest are being separated, change the polarity of the electrodes, since they have a net positive charge.</p> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td style="text-align: center;" width="87" valign="top"><strong>10x Buffer Volume</strong></td> <td style="text-align: center;" width="87" valign="top"><strong>Catalog Number </strong></td> </tr> <tr class="pd_colorbackground"> <td style="text-align: center;" valign="top">1 L</td> <td style="text-align: center;" valign="top">1610734</td> </tr> <tr> <td style="text-align: center;" valign="top">5 L cube</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/1610771-10x-tris-glycine-buffer-for-western-blots-native-gels?ID=1610771">1610771</a></td> </tr> </tbody> </table> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/product/precisionab-validated-western-blotting-antibodies">PrecisionAb&trade; Validated Western Blotting Antibodies</a></li> <li>Native Sample Buffer (<a href="//en-us/sku/1610738-native-sample-buffer-for-protein-gels-30-ml?ID=1610738">1610738</a>)</li> <li>Criterion Staining/Blotting Trays with lids (<a href="/en-us/sku/3459921-criterion-staining-blotting-trays">3459921</a>)</li> <li>10x Phosphate Buffered Saline (PBS) (<a href="/en-us/sku/1610780-10x-phosphate-buffered-saline-pbs?ID=1610780">1610780</a>)</li> <li>10x Tris Buffered Saline (TBS) (<a href="/en-us/sku/1706435-10x-tris-buffered-saline-tbs?ID=1706435-tbs">1706435</a>)</li> <li>1x Phosphate Buffered Saline (PBS) with 1% Casein (<a href="/en-us/sku/1610783-1x-phosphate-buffered-saline-pbs-with-1-casein">1610783</a>)</li> <li>1x Tris Buffered Saline (TBS) with 1% Casein (<a href="/en-us/sku/1610782-1x-tris-buffered-saline-tbs-with-1-casein?ID=1610782">1610782</a>)</li> <li>Blotting-Grade Blocker, nonfat dry milk (<a href="/en-us/sku/1706404-blotting-grade-blocker">1706404</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/protein-gels">Protein Gels</a></li> <li><a href="/en-us/category/western-blotting-membranes-filter-paper?ID=ef19d64e-5909-450d-9d71-cc5e59555033">Western Blotting Membranes and Filter Paper</a></li> <li><a href="/en-us/category/semi-dry-rapid-blotting-systems">Semi-Dry and Rapid Blotting Systems</a></li> <li><a href="/en-us/category/wet-tank-blotting-systems">Wet/Tank Blotting Systems</a></li> <li><a href="/en-us/category/power-supplies?ID=5f7dc809-5983-461f-90c9-4610c7d6f780">Power Supplies</a></li> <li><a href="/en-us/category/transfer-buffers">Transfer Buffers</a></li> <li><a href="/en-us/category/immunodetection-reagents-kits?ID=534c009d-cd86-4b33-956c-674d5a6ae054">Immunodetection Reagents and Kits</a></li> <li><a href="/en-us/category/gel-imaging-systems?ID=d6f93330-755b-410c-9c9c-77da24d51eab">Gel and Western Imaging Systems</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Mini-PROTEAN Precast Gels <a href="/webroot/web/pdf/lsr/literature/Bulletin_1658100.pdf" target="_blank" rel="noopener noreferrer">Instruction Manual and Application Guide</a></li> <li>Criterion Gel Application Guide <a href="/webroot/web/pdf/lsr/literature/4110001.pdf" target="_blank" rel="noopener noreferrer">Instruction Manual</a></li> <li>Mini Trans-Blot<sup>&reg;</sup> Cell <a href="/webroot/web/pdf/lsr/literature/M1703930.pdf" target="_blank" rel="noopener noreferrer">Instruction Manual</a></li> <li>Trans-Blot SD Cell <a href="/webroot/web/pdf/lsr/literature/4006066B.pdf" target="_blank" rel="noopener noreferrer">Quick Reference Guide</a></li> <li>Trans-Blot SD Semi-Dry Transfer Cell <a href="/webroot/web/pdf/lsr/literature/M1703940.pdf" target="_blank" rel="noopener noreferrer">Instruction Manual</a></li> </ul> <h2 class="descHeader">Online Applications</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-protein-electrophoresis?ID=LUSOVO47B">Protein Electrophoresis</a></li> <li><a href="/en-us/applications-technologies/introduction-western-blotting?ID=LUSPPAKG4">Western Blotting</a></li> <li><a href="/en-us/applications-technologies/imaging-analysis">Imaging and Analysis</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated 1610734.html /webroot/web/html/lsr/products/SKUs/autopopulated/1610734.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610734&CartQuote=QT&quantity= 1425 false true false $30.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610734&AddToDefaultWishList=true&quantity= true false true 12161700 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610734&quantity= 1610734
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10x Tris/Glycine Buffer for Western Blots and Native Gels

1610771
Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water

1 1610771 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0771_0921120339765.xml 1610771 /default/main/bio-rad/WORKAREA/default 09/21/09 12:03 PM en_US jcc1toym 2017-11-14 07:08:21 true true internet/product_SKU jcc1toym templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0771_0921120339765.xml LSR 2019-05-04 18:50:03 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1556922622861 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 18:50:03 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0771_0921120339765.xml templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0771_0921120339765.xml 1610771-10x-tris-glycine-buffer-for-western-blots-native-gels 1610771 10x Tris/Glycine 10x Tris/Glycine Buffer for Western Blots and Native Gels Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water 5 L cube, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, pH 8.3 Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/161-0771_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/161-0771_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/161-0771_feature.jpg 10x Tris/Glycine Buffer for Western Blots and Native Gels ,VN RepEx 161-0771 5 L cube, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, pH 8.3 161-0771, 1610771, 10x, tris/glycine, native, running, buffer, tris, glycine, polyacrylamide, page, protein, gel, electrophoresis, buffers, reagent, reagents 09/21/09 12:03 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <p>Use 10x Tris/Glycine Buffer as a transfer buffer for western blots or as a running buffer for native protein gel electrophoresis.</p> <ul> <li>For tank or semi-dry blotting for SDS PAGE gels, usually with the addition of 20% methanol</li> <li>For tank blotting of native gels, without methanol</li> <li>As a running buffer for native gels</li> </ul> <h2 class="descHeader">Precast gels that can be used for native electrophoresis</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td style="text-align: left;" width="87" valign="top"><strong>Precast Gel Type</strong></td> <td style="text-align: center;" width="87" valign="top"><strong>Size</strong></td> <td style="text-align: left;" width="87" valign="top"><strong>Gel Chemistry</strong></td> </tr> <tr class="pd_colorbackground"> <td style="text-align: left;" valign="top"><a href="/en-us/product/mini-protean-precast-gels/mini-protean-tgx-precast-gels">Mini-PROTEAN<sup>&reg;</sup> TGX&trade;</a></td> <td style="text-align: center;" valign="top">Mini</td> <td style="text-align: left;" rowspan="2" valign="top">Laemmli-like, long shelf life, fast separation with high resolution</td> </tr> <tr class="pd_colorbackground"> <td style="text-align: left;" valign="top"><a href="/en-us/product/protein-gels/criterion-precast-gels/criterion-tgx-precast-gels">Criterion&trade; TGX&trade;</a></td> <td style="text-align: center;" valign="top">Midi</td> </tr> <tr> <td style="text-align: left;" valign="top"><a href="/en-us/product/mini-protean-precast-gels/mini-protean-tgx-stain-free-precast-gels">Mini-PROTEAN<sup>&reg;</sup> TGX Stain-Free&trade;</a></td> <td style="text-align: center;" valign="top">Mini</td> <td style="text-align: left;" rowspan="2" valign="top">Laemmli-like, long shelf life, fast separation with high resolution, unique trihalo compounds for rapid fluorescent protein detection</td> </tr> <tr> <td style="text-align: left;" valign="top"><a href="/en-us/product/criterion-precast-gels/criterion-tgx-stain-free-precast-gels">Criterion&trade; TGX Stain-Free&trade;</a></td> <td style="text-align: center;" valign="top">Midi</td> </tr> <tr class="pd_colorbackground"> <td style="text-align: left;" valign="top"><a href="/en-us/product/criterion-tris-hcl-precast-gels">Criterion&trade; Tris-HCl</a></td> <td style="text-align: center;" valign="top">Midi</td> <td style="text-align: left;" valign="top">Standard Laemmli</td> </tr> <tr> <td style="text-align: left;" valign="top"><a href="/en-us/product/criterion-precast-gels/criterion-stain-free-tris-hcl-gels">Criterion Stain Free&trade; Tris-HCl</a></td> <td style="text-align: center;" valign="top">Midi</td> <td style="text-align: left;" valign="top">Standard Laemmli, unique trihalo compounds for rapid fluorescent protein detection</td> </tr> <tr class="pd_colorbackground"> <td style="text-align: left;" valign="top"><a href="/en-us/product/criterion-precast-gels/criterion-xt-tris-acetate-precast-gels">Criterion XT Tris-Acetate</a></td> <td style="text-align: center;" valign="top">Midi</td> <td style="text-align: left;" valign="top">Discontinuous buffer ion fronts form moving boundaries to stack, and then separate proteins</td> </tr> </tbody> </table> <p class="caption" style="margin-top:-10px">Note: Most proteins have an acidic or slightly basic pI (~3&ndash;8) and are run with the power supply connected to the electrophoresis chamber as for SDS-PAGE. If more basic proteins (pl &gt;8.5) of interest are being separated, change the polarity of the electrodes, since they have a net positive charge.</p> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td style="text-align: center;" width="87" valign="top"><strong>10x Buffer Volume</strong></td> <td style="text-align: center;" width="87" valign="top"><strong>Catalog Number </strong></td> </tr> <tr class="pd_colorbackground"> <td style="text-align: center;" valign="top">1 L</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/1610734-10x-tris-glycine-buffer-for-western-blots-native-gels?ID=1610734">1610734</a></td> </tr> <tr> <td style="text-align: center;" valign="top">5 L cube</td> <td style="text-align: center;" valign="top">1610771</td> </tr> </tbody> </table> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/product/precisionab-validated-western-blotting-antibodies">PrecisionAb&trade; Validated Western Blotting Antibodies</a></li> <li>Native Sample Buffer (<a href="/en-us/sku/1610738-native-sample-buffer-for-protein-gels-30-ml?ID=1610738">1610738</a>)</li> <li>Criterion Staining/Blotting Trays with lids (<a href="/en-us/sku/3459921-criterion-staining-blotting-trays">3459921</a>)</li> <li>10x Phosphate Buffered Saline (PBS) (<a href="/en-us/sku/1610780-10x-phosphate-buffered-saline-pbs?ID=1610780">1610780</a>)</li> <li>10x Tris Buffered Saline (TBS) (<a href="/en-us/sku/1706435-10x-tris-buffered-saline-tbs?ID=1706435-tbs">1706435</a>)</li> <li>1x Phosphate Buffered Saline (PBS) with 1% Casein (<a href="/en-us/sku/1610783-1x-phosphate-buffered-saline-pbs-with-1-casein">1610783</a>)</li> <li>1x Tris Buffered Saline (TBS) with 1% Casein (<a href="/en-us/sku/1610782-1x-tris-buffered-saline-tbs-with-1-casein?ID=1610782">1610782</a>)</li> <li>Blotting-Grade Blocker, nonfat dry milk (<a href="/en-us/sku/1706404-blotting-grade-blocker">1706404</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/protein-gels">Protein Gels</a></li> <li><a href="/en-us/category/western-blotting?ID=9324fd3c-6af4-4551-831e-8db6fa3f3452-membranes-filter-paper">Western Blotting Membranes and Filter Paper</a></li> <li><a href="/en-us/category/semi-dry-rapid-blotting-systems">Semi-Dry and Rapid Blotting Systems</a></li> <li><a href="/en-us/category/wet-tank-blotting-systems">Wet/Tank Blotting Systems</a></li> <li><a href="/en-us/category/power-supplies?ID=5f7dc809-5983-461f-90c9-4610c7d6f780">Power Supplies</a></li> <li><a href="/en-us/category/transfer-buffers">Transfer Buffers</a></li> <li><a href="/en-us/category/immunodetection-reagents-kits?ID=534c009d-cd86-4b33-956c-674d5a6ae054">Immunodetection Reagents and Kits</a></li> <li><a href="/en-us/category/gel-imaging-systems?ID=d6f93330-755b-410c-9c9c-77da24d51eab">Gel and Western Imaging Systems</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Mini-PROTEAN Precast Gels <a href="/webroot/web/pdf/lsr/literature/Bulletin_1658100.pdf" target="_blank">Instruction Manual and Application Guide</a></li> <li>Criterion Gel Application Guide <a href="/webroot/web/pdf/lsr/literature/4110001.pdf" target="_blank">Instruction Manual</a></li> <li>Mini Trans-Blot<sup>&reg;</sup> Cell <a href="/webroot/web/pdf/lsr/literature/M1703930.pdf" target="_blank">Instruction Manual</a></li> <li>Trans-Blot SD Cell <a href="/webroot/web/pdf/lsr/literature/4006066B.pdf" target="_blank">Quick Reference Guide</a></li> <li>Trans-Blot SD Semi-Dry Transfer Cell <a href="/webroot/web/pdf/lsr/literature/M1703940.pdf" target="_blank">Instruction Manual</a></li> </ul> <h2 class="descHeader">Online Applications</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-protein-electrophoresis?ID=LUSOVO47B">Protein Electrophoresis</a></li> <li><a href="/en-us/applications-technologies/introduction-western-blotting?ID=LUSPPAKG4">Western Blotting</a></li> <li><a href="/en-us/applications-technologies/imaging-analysis">Imaging and Analysis</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated 1610771.html /webroot/web/html/lsr/products/SKUs/autopopulated/1610771.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610771&CartQuote=QT&quantity= 1425 false true false $102.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610771&AddToDefaultWishList=true&quantity= true false true 12161700 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610771&quantity= 1610771
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10x Tris/CAPS Buffer for Semi-Dry Blotting

1610778
1 L, 10x Tris/CAPS

2 1610778 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0778_0921120404854.xml 1610778 /default/main/bio-rad/WORKAREA/default 09/21/09 12:04 PM en_US ja9sm55u 2017-11-14 07:08:21 true true internet/product_SKU ja9sm55u templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0778_0921120404854.xml LSR 2019-05-04 18:50:06 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1556908422292 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 18:50:06 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0778_0921120404854.xml templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0778_0921120404854.xml 1610778-10x-tris-caps-buffer-for-semi-dry-blotting 1610778 10x Tris/CAPS 10x Tris/CAPS Buffer for Semi-Dry Blotting 1 L, 10x Tris/CAPS 1 L, transfer buffer, anode buffer: 60mM Tris, 40 mM CAPS, 15% methanol, pH 9.6; cathode buffer: 60 mM Tris, 40 MM CAPS, 0.1% (w/v) SDS, pH 9.6 1 L, 10x Tris/CAPS /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/161-0778_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/161-0778_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/161-0778_feature.jpg 10x Tris/CAPS Buffer for Semi-Dry Blotting ,VN RepEx 161-0778 1 L, transfer buffer, anode buffer: 60mM Tris, 40 mM CAPS, 15% methanol, pH 9.6; cathode buffer: 60 mM Tris, 40 MM CAPS, 0.1% (w/v) SDS, pH 9.6 1610778, 10x, tris/caps, tris, caps, premixed, buffer, transfer, western, blot, blots, blotting 09/21/09 12:04 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <p>Use 10x Tris/CAPS Buffer for western transfer when using a semi-dry blotting apparatus. Prior to use, methanol needs to be added to a final concentration of 15% to the anode buffer, and SDS needs to be added to a final concentration of 0.1% to the cathode buffer.</p> <ul> <li>Discontinuous buffer system increases transfer efficiency</li> <li>Transfers take place in as little as 15&ndash;60 min</li> <li>Economical, low buffer requirements</li> </ul> <h2 class="descHeader">Related Products</h2> <ul> <li>Trans-Blot<sup>&reg;</sup> SD Semi-Dry Electrophoretic Transfer Cell (<a href="/en-us/sku/1703940-trans-blot-sd-semi-dry-electrophoretic-transfer-cell">1703940</a>)</li> <li><a href="/en-us/product/precisionab-validated-western-blotting-antibodies">PrecisionAb&trade; Validated Western Blotting Antibodies</a></li> <li>Criterion&trade; Staining/Blotting Trays with lids (<a href="/en-us/sku/3459921-criterion-staining-blotting-trays">3459921</a>)</li> <li>10x Phosphate Buffered Saline (PBS) (<a href="/en-us/sku/1610780-10x-phosphate-buffered-saline-pbs?ID=1610780">1610780</a>)</li> <li>10x Tris Buffered Saline (TBS) (<a href="/en-us/sku/1706435-10x-tris-buffered-saline-tbs?ID=1706435">1706435</a>)</li> <li>1x Phosphate Buffered Saline (PBS) with 1% Casein (<a href="/en-us/sku/1610783-1x-phosphate-buffered-saline-pbs-with-1-casein?ID=1610783">1610783</a>)</li> <li>1x Tris Buffered Saline (TBS) with 1% Casein (<a href="/en-us/sku/1610782-1x-tris-buffered-saline-tbs-with-1-casein?ID=1610782">1610782</a>)</li> <li>Blotting-Grade Blocker, nonfat dry milk (<a href="/en-us/sku/1706404-blotting-grade-blocker">1706404</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/protein-gels">Protein Gels</a></li> <li><a href="/en-us/category/western-blotting?ID=9324fd3c-6af4-4551-831e-8db6fa3f3452-membranes-filter-paper">Western Blotting Membranes and Filter Paper</a></li> <li><a href="/en-us/category/semi-dry-rapid-blotting-systems">Semi-Dry and Rapid Blotting Systems</a></li> <li><a href="/en-us/category/power-supplies?ID=5f7dc809-5983-461f-90c9-4610c7d6f780">Power Supplies</a></li> <li><a href="/en-us/category/transfer-buffers">Transfer Buffers</a></li> <li><a href="/en-us/category/immunodetection-reagents-kits?ID=534c009d-cd86-4b33-956c-674d5a6ae054">Immunodetection Reagents and Kits</a></li> <li><a href="/en-us/category/gel-imaging-systems?ID=d6f93330-755b-410c-9c9c-77da24d51eab">Gel and Western Imaging Systems</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Trans-Blot SD Cell <a href="/webroot/web/pdf/lsr/literature/4006066B.pdf" target="_blank">Quick Reference Guide</a></li> <li>Trans-Blot SD Semi-Dry Transfer Cell <a href="/webroot/web/pdf/lsr/literature/M1703940.pdf" target="_blank">Instruction Manual</a></li> </ul> <h2 class="descHeader">Online Applications</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-western-blotting?ID=LUSPPAKG4">Western Blotting</a></li> <li><a href="/en-us/applications-technologies/introduction-protein-electrophoresis?ID=LUSOVO47B">Protein Electrophoresis</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated 1610778.html /webroot/web/html/lsr/products/SKUs/autopopulated/1610778.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610778&CartQuote=QT&quantity= 1425 false true false $80.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610778&AddToDefaultWishList=true&quantity= true false true 12161700 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610778&quantity= 1610778
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(Discontinued) 20x SSC

1610774
1 L, 20x SSC, blot transfer and processing buffer, pH 7.0

This product is no longer available. Please contact technical support for more information on replacement product.

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(Discontinued) 20x SSC

1610775
5 L cube, 20x SSC, blot transfer and processing buffer, pH 7.0

This product is no longer available. Please contact technical support for more information on replacement product.

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10x Phosphate Buffered Saline (PBS)

1610780
1 L, 10x PBS

5 1610780 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0780_0921120404805.xml 1610780 /default/main/bio-rad/WORKAREA/default 09/21/09 12:04 PM en_US ja9sm53w 2017-11-14 07:08:21 true true internet/product_SKU ja9sm53w templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0780_0921120404805.xml LSR 2019-05-04 18:50:01 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1556908422337 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 18:50:01 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0780_0921120404805.xml templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0780_0921120404805.xml 1610780-10x-phosphate-buffered-saline-pbs 1610780 10x Phosphate Buffered Saline 10x Phosphate Buffered Saline (PBS) 1 L, 10x PBS 1 L, detergent, 10mM sodium phosphate, 150 mM NaCl, pH 7.4 1 L, 10x PBS /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/161-0780_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/161-0780_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/161-0780_feature.jpg 10x Phosphate Buffered Saline (PBS) ,VN RepEx 161-0780 1 L, detergent, 10mM sodium phosphate, 150 mM NaCl, pH 7.4 161-0780, 1610780, 10x, phosphate, buffered, saline, pbs, buffer, buffers 09/21/09 12:04 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <p>Use 10x Phosphate Buffered Saline (PBS) for washing western blots and as a wash buffer in other applications. When using alkaline phosphatase as a reporter in western blots, it is recommended that Phosphate Buffered Saline is replaced with Tris Buffered Saline (TBS).</p> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/product/precisionab-validated-western-blotting-antibodies">PrecisionAb&trade; Validated Western Blotting Antibodies</a></li> <li>Criterion&trade; Staining/Blotting Trays with Lids (<a href="/en-us/sku/3459921-criterion-staining-blotting-trays">3459921</a>)</li> <li>10x Tris Buffered Saline (<a href="/en-us/sku/1706435-10x-tris-buffered-saline-tbs?ID=1706435">1706435</a>)</li> <li>1x Phosphate Buffered Saline (PBS) with 1% Casein (<a href="/en-us/sku/1610783-1x-phosphate-buffered-saline-pbs-with-1-casein?ID=1610783">1610783</a>)</li> <li>1x Tris Buffered Saline (TBS) with 1% Casein (<a href="/en-us/sku/1610782-1x-tris-buffered-saline-tbs-with-1-casein?ID=1610782">1610782</a>)</li> <li>Blotting-Grade Blocker, nonfat dry milk (<a href="/en-us/sku/1706404-blotting-grade-blocker">1706404</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/protein-gels">Protein Gels</a></li> <li><a href="http://www.bio-rad.com/en-us/category/western-blotting?ID=9324fd3c-6af4-4551-831e-8db6fa3f3452 ">Western Blotting Membranes and Filter Paper</a></li> <li><a href="/en-us/category/semi-dry-rapid-blotting-systems">Semi-Dry and Rapid Blotting Systems</a></li> <li><a href="/en-us/category/wet-tank-blotting-systems">Wet/Tank Blotting Systems</a></li> <li><a href="/en-us/category/power-supplies?ID=5f7dc809-5983-461f-90c9-4610c7d6f780">Power Supplies</a></li> <li><a href="/en-us/category/transfer-buffers">Transfer Buffers</a></li> <li><a href="/en-us/category/immunodetection-reagents-kits?ID=534c009d-cd86-4b33-956c-674d5a6ae054">Immunodetection Reagents and Kits</a></li> <li><a href="/en-us/category/gel-imaging-systems?ID=d6f93330-755b-410c-9c9c-77da24d51eab">Gel and Western Imaging Systems</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Mini-PROTEAN<sup>&reg;</sup> Precast Gels <a href="/webroot/web/pdf/lsr/literature/Bulletin_1658100.pdf" target="_blank">Instruction Manual and Application Guide</a></li> <li>Criterion Gel Application Guide <a href="/webroot/web/pdf/lsr/literature/4110001.pdf" target="_blank">Instruction Manual</a></li> <li>Mini Trans-Blot<sup>&reg;</sup> Cell <a href="/webroot/web/pdf/lsr/literature/M1703930.pdf" target="_blank">Instruction Manual</a></li> <li>Trans-Blot SD Cell <a href="/webroot/web/pdf/lsr/literature/4006066B.pdf" target="_blank">Quick Reference Guide</a></li> <li>Trans-Blot SD Semi-Dry Transfer Cell <a href="/webroot/web/pdf/lsr/literature/M1703940.pdf" target="_blank">Instruction Manual</a></li> </ul> <h2 class="descHeader">Online Applications</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-protein-electrophoresis?ID=LUSOVO47B">Protein Electrophoresis</a></li> <li><a href="/en-us/applications-technologies/introduction-western-blotting?ID=LUSPPAKG4">Western Blotting</a></li> <li><a href="/en-us/applications-technologies/imaging-analysis">Imaging and Analysis</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated 1610780.html /webroot/web/html/lsr/products/SKUs/autopopulated/1610780.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610780&CartQuote=QT&quantity= 1425 false true false $47.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610780&AddToDefaultWishList=true&quantity= true false true 12161700 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610780&quantity= 1610780
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10x Tris Buffered Saline (TBS)

1706435
1 L, 10x TBS

6 1706435 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6435_0921120404761.xml 1706435 /default/main/bio-rad/WORKAREA/default 09/21/09 12:04 PM en_US juso9m9q 2018-02-01 00:22:46 true true internet/product_SKU juso9m9q templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6435_0921120404761.xml LSR 2019-05-04 18:50:07 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1556908428679 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 18:50:07 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6435_0921120404761.xml templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6435_0921120404761.xml 1706435-10x-tris-buffered-saline-tbs 1706435 10x Tris Buffered Saline 10x Tris Buffered Saline (TBS) 1 L, 10x TBS 1 L, 10x TBS 1 L, 10x TBS /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/170-6435_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/170-6435_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/170-6435_feature.jpg 10x Tris Buffered Saline (TBS) preview ,VN RepEx 170-6435 1 L, 10x TBS 1706435, 10x, tris, buffered, saline, tbs, wash, buffer, blots, western 09/21/09 12:04 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <p>Choose 10x Tris Buffered Saline (TBS) for washing western blots. Unlike Phosphate Buffered Saline (PBS), this buffer does not inhibit alkaline phosphatase.</p> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/product/precisionab-validated-western-blotting-antibodies">PrecisionAb&trade; Validated Western Blotting Antibodies</a></li> <li>Criterion&trade; Staining/Blotting Trays with Lids (<a href="/en-us/sku/3459921-criterion-staining-blotting-trays">3459921</a>)</li> <li>10x Phosphate Buffered Saline (PBS) (<a href="/en-us/sku/1610780-10x-phosphate-buffered-saline-pbs?ID=1610780">1610780</a>)</li> <li>1x Phosphate Buffered Saline (PBS) with 1% Casein (<a href="/en-us/sku/1610783-1x-phosphate-buffered-saline-pbs-with-1-casein">1610783</a>)</li> <li>1x Tris Buffered Saline (TBS) with 1% Casein (<a href="/en-us/sku/1610782-1x-tris-buffered-saline-tbs-with-1-casein?ID=1610782">1610782</a>) </li> <li>Blotting-Grade Blocker, nonfat dry milk (<a href="/en-us/sku/1706404-blotting-grade-blocker">1706404</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/protein-gels">Protein Gels</a></li> <li><a href="http://www.bio-rad.com/en-us/category/western-blotting?ID=9324fd3c-6af4-4551-831e-8db6fa3f3452">Western Blotting Membranes and Filter Paper</a></li> <li><a href="/en-us/category/semi-dry-rapid-blotting-systems">Semi-Dry and Rapid Blotting Systems</a></li> <li><a href="/en-us/category/wet-tank-blotting-systems">Wet/Tank Blotting Systems</a></li> <li><a href="/en-us/category/power-supplies?ID=5f7dc809-5983-461f-90c9-4610c7d6f780">Power Supplies</a></li> <li><a href="/en-us/category/transfer-buffers">Transfer Buffers</a></li> <li><a href="/en-us/category/immunodetection-reagents-kits?ID=534c009d-cd86-4b33-956c-674d5a6ae054">Immunodetection Reagents and Kits</a></li> <li><a href="/en-us/category/gel-imaging-systems?ID=d6f93330-755b-410c-9c9c-77da24d51eab">Gel and Western Imaging Systems</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Mini-PROTEAN<sup>&reg;</sup> Precast Gels <a href="/webroot/web/pdf/lsr/literature/Bulletin_1658100.pdf" target="_blank">Instruction Manual and Application Guide</a></li> <li>Criterion Gel Application Guide <a href="/webroot/web/pdf/lsr/literature/4110001.pdf" target="_blank">Instruction Manual</a></li> <li>Mini Trans-Blot<sup>&reg;</sup> Cell <a href="/webroot/web/pdf/lsr/literature/M1703930.pdf" target="_blank">Instruction Manual</a></li> <li>Trans-Blot SD Cell <a href="/webroot/web/pdf/lsr/literature/4006066B.pdf">Quick Reference Guide</a></li> <li>Trans-Blot SD Semi-Dry Transfer Cell <a href="/webroot/web/pdf/lsr/literature/M1703940.pdf" target="_blank">Instruction Manual</a></li> </ul> <h2 class="descHeader">Online Applications</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-protein-electrophoresis?ID=LUSOVO47B">Protein Electrophoresis</a></li> <li><a href="/en-us/applications-technologies/introduction-western-blotting?ID=LUSPPAKG4">Western Blotting</a></li> <li><a href="/en-us/applications-technologies/imaging-analysis">Imaging and Analysis</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated 1706435.html /webroot/web/html/lsr/products/SKUs/autopopulated/1706435.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1706435&CartQuote=QT&quantity= 1425 false true false $43.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1706435&AddToDefaultWishList=true&quantity= true false true 12161700 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1706435&quantity= 1706435
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