NGC 100 Medium-Pressure Chromatography Systems

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Overview

Optimized for purifications at the mg to g scale, these preconfigured 100 ml NGC Chromatography Systems are designed to meet a range of throughput and application needs. For scale-up analytical and preparative chromatography.

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Description

Description

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The family of NGC Medium-Pressure Chromatography Systems fit your throughput requirements when purifying biomolecules for analytical- and preparative-scale applications. Each system is composed of interchangeable modules that confer different system capabilities.

The six preconfigured NGC Liquid Chromatography Systems listed below are designed with increasing automation and throughput to serve a wide range of laboratory needs:

  • NGC Quest 100 (7880002) and NGC Quest 100 Plus Systems (7880004) — for separating biomolecules at high resolution using accurate gradients
  • NGC Scout 100 (7880006) and NGC Scout 100 Plus Systems (7880008) — for medium throughput applications requiring rapid scouting of protein purification conditions with automated gradients and buffer preparation
  • NGC Discover 100 (7880010) and NGC Discover 100 Pro Systems (7880012) — for higher throughput applications with rapid, robust automation and a need for method development

For an overview of the features and capabilities of the six NGC Chromatography Systems, view the NGC 100 System comparison chart and watch the NGC System Tour.

The NGC Systems are composed of interchangeable modules that confer different system capabilities. Browse the module selection guide to help you decide which system is best for you, or use our online tool to configure your personalized NGC Instrument.

Features and Benefits of the NGC 100 ml Chromatography Systems

Flexible, customizable chromatography systems to suit both your application and workflow needs:

  • The guided fluidics selection tool (patent pending), coupled with the module discovery system, enables a single-click application-based system setup through automated module function recognition
  • The Tier Rotate Feature allows optimal placement of valves and detectors to minimize the flow path
  • Compact footprint — fits in a refrigerator, lab bench, or coldroom
  • Point-to-Plumb Feature shows the step-by-step plumbing of the system using LEDs
  • ChromLab Software provides powerful system control, automated methods, and analysis options

Applications and Uses of Medium-Pressure Chromatography Systems

Other NGC Chromatography Systems

Bio-Rad also offers NGC Chromatography Systems with integrated 10 ml system pumps and NGC Fraction Collector as well as a variety of chromatography columns and resins.

Compatible Chromatography Columns

Designed specifically for NGC Chromatography Systems, these prepacked high-resolution columns work at high pressures and are optimized for the separation of biomolecules at fast flow rates.

 

More Information

 

Upgrade your NGC Chromatography System by adding more modular components to match your changing throughput and application requirements:

See how the NGC System benefits University of California researchers by increasing throughput and making it easier for students to become experts at protein purification.

Ordering

Ordering

items

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7880002
Includes 100 ml/min pumps, single-wavelength (UV) and conductivity detection, and automated sample injection, for the purification of proteins
ngc-100-medium-pressure-chromatography-systems-788-0004-thumb.jpg
7880004
Includes 100 ml/min pumps, multi-wavelength (UV/Vis) and conductivity detection, and automated sample injection, for the purification of proteins
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7880006
Includes 100 ml/min pumps, single-wavelength (UV) and conductivity detection, buffer blending options, for rapid pH scouting during sample purification
ngc-scout-100-plus-system-SKU-788-0008-thumb.jpg
7880008
Includes 100 ml/min pumps, multi-wavelength (UV/Vis) and conductivity detection, buffer blending options, and a pH valve, for rapid pH scouting during the purification of proteins, peptides, and nucleic acids
ngc-discover-100-system-SKU-788-0010-thumb.jpg
7880010
Includes 100 ml/min pumps, multi-wavelength (UV/Vis) and conductivity detection, expanded scouting options, large-sample injection, outlet valve, and column switching of up to 5 columns, for method development
ngc-discover-100-system-SKU-788-0012-thumb.jpg
7880012
Includes 100 ml/min pumps, multi-wavelength (UV/Vis) and conductivity detection, expanded scouting options, large-sample injection, outlet valve and column switching of up to 5 columns, for method development

Accessories

Accessories

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7410002
100/240 V, fraction collector, includes power cord, rack set F1 (2 x flatpack, 13 mm), BioFrac Diverter Valve, fittings kit
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(Discontinued) Computer
7884015
PC for ChromLab™ Software and database, communicates with and stores data generated by the NGC™ System
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7884016
Pkg of 1, enables analog to digital signal conversion and connection to external detectors and third party autosamplers such as the C-96 Autosampler
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12012531
Pkg of 1, includes a 10 mm UV flow cell with an integrated conductivity monitor compatible with the NGC Multi-Wavelength II Detector Module (12010343) for analytical applications.
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12012532
Pkg of 1, includes a 5 mm UV flow cell with an integrated conductivity monitor compatible with the NGC Multi-Wavelength II Detector Module. (12010343)
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12012533
Pkg of 1, includes a 2 mm UV flow cell with an integrated conductivity monitor compatible with the NGC Multi-Wavelength II Detector Module (12010343) for preparative applications.

Fittings

Fittings

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(Discontinued) NGC™ Fittings Kit
7884017
Pkg of 1, kit includes multiple fittings, fittings maker, fittings tightener, ferrules, nuts, screwdrivers, pump head keys, and syringes required to set up, prepare, and prime NGC Systems
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7320113
Low-pressure system fittings kit, includes 1/4–28 female to male and 1/4–28 female to female luers to connect 1 cartridge to BioLogic DuoFlow™ and NGC Systems
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(Discontinued) Tefzel Plug
7500563
Pkg of 5, 1/4–28 male connection, to plug unused ports on valves and columns, for use with BioLogic DuoFlow™ and NGC™ Systems
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7885009
Pkg of 4 (qty 2 pairs — each with one 1/4-28 F to 10-32 M adaptor and one 1/4-28 M to 10-32 F adaptor)
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7500565
Pkg of 2, fittings to connect one Econo-Column (luer) Column to BioLogic DuoFlow and NGC Systems
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7885010
Pkg of 10, includes five slip luer F to 10-32 M adaptors and five quick-connect luer M to 10-32 F adaptors
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(Discontinued) Tefzel Cap
7500559
Pkg of 5, Tefzel caps, 1/4–28 female connection, to plug unused tubing, for use with BioLogic DuoFlow™ and NGC™ Systems
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7500568

Fittings kit, includes 2 nuts and 4 ferrules to connect UNO Column to an HPLC system, for use with BioLogic DuoFlow and NGC Systems

Modules

Modules

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12010343

Pkg of 1, includes UV/Vis and conductivity detector kit with 5 mm flow cell, necessary tubing, and fittings, for protein, peptide, nucleic acid, and chromophore detection

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7884008
Pkg of 1, includes UV/conductivity detector kit with necessary tubing and fittings, for nucleotide and protein detection, and salt gradient monitoring
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7884007
Pkg of 1, kit includes necessary tubing, fittings, and a sample injection port, for automated application of small sample volume samples
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7884006
Pkg of 1, kit includes necessary tubing and fittings, for automated switching between multiple buffers and samples during method development
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7884013
Pkg of 1, kit includes necessary tubing and fittings for automated fraction collection of large-volume fractions with up to 12 vessels
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7884010
Pkg of 1, kit includes necessary tubing and fittings, for inline buffer preparation and pH gradient generation for quick pH scouting
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7884026
Pkg of 1, kit includes the necessary tubing and fittings to accommodate up to 5 columns, for column scouting and reverse flow applications using NGC 100 Chromatography Systems
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7884004
Pkg of 1, includes 100 ml/min sample pump kit with the necessary tubing and fittings, for automated large-volume sample application

Documents

Documents

Number Description Options
3232
Scale Separation: Optimizing the Bio-Rad NGC Chromatography System for Low Volume Size Exclusion Chromatography
3229
Optimizing the NGC Chromatography System for Cryo-EM Applications Quick Guide
7260
Microbial Challenge and Sanitization of the NGC Discover 10 Pro System Using Sodium Hydroxide
7194
Integrating the CETAC MVX-7100 Autosampler with the NGC Chromatography System Protocol, Ver A
7208
NGC Chromatography System Connection Guide for Wyatt Instrumentation Protocol
[ Add to Cart (Free) ]
3211
Purification of Cas9 Nuclease with a Two-Step Chromatographic Process Application Note
7195
Integrating the CETAC MVX-7100 Autosampler with the NGC Chromatography System Quick Guide, Ver A
7205
NGC Liquid Chromatography System Quick Guide
7137
A Model System for Fast Screening of Optimal Protein Purification Conditions
7031
Integrating the ALIAS Autosampler with the NGC™ Chromatography System Protocol
6494
Protein Purification Workflow with the NGC Chromatography System and Stain-Free Gels Brochure
[ Add to Cart (Free) ]
6286
NGC Chromatography Systems Brochure
[ Add to Cart (Free) ]
7209
Chemical Compatibility with the NGC Chromatography System Flier
[ Add to Cart (Free) ]
6326
NGC Chromatography Systems Module Selection Guide
[ Add to Cart (Free) ]
10000049090
NGC Chromatography Systems and ChromLab Software Installation Guide Version 6.1
10000049091
NGC Chromatography Systems and ChromLab Software Instrument Guide Version 6.1
10000049092
NGC Chromatography Systems and ChromLab Software User Guide Version 6.1
6608
NGC Chromatography Systems for Protein Purification in Drug Discovery and Development Brochure
[ Add to Cart (Free) ]
6963
NGC Fraction Collector Brochure
6674
NGC Chromatography Systems Multidimensional (Multi-D) Plumbing Guide
6896
Integrating the CETAC ASX-560 Autosampler with the NGC™ Chromatography System
6879
Increasing Thoughput of a Tandem mAb Purification by Adding a High-Volume Autosampler to the NGC Chromatography System
6377
Chromatography Success Guide
6701
Multidimensional (Multi-D) Chromatography Success Guide, Ver B
7277
Determination of Molecular Weight and Disperity of Antibody Samples Using Bio-Rad's NGC Chromatography System and Wyatt's MALS and RI Detectors
6694
Advantages of Multidimensional (Multi-D) Chromatography Using the NGC™ Chromatography System over Traditional Sequential Chromatography, Ver B
6805
Proven Method for Confirming Protein Purity and Yield Brochure
[ Add to Cart (Free) ]
6843
NGC Chromatography System Applications Publication List
[ Add to Cart (Free) ]
6675
ChromLab™ Software Product Information Sheet
3251
Cas9 Nuclease Preparation Using EconoFit Columns and the NGC Chromatography System Application Note