iQ-Check Enterobacteriaceae PCR Detection Kit

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Overview

Complete sample preparation and PCR kit for the detection of Enterobacteriaceae in food and environmental samples. Next day results for all samples.

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Description

Description

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Enterobacteriaceae (EB), often used as indicators for hygiene process criteria, is a large family of gram-negative bacteria that encompasses some of the most well-known food pathogens like Salmonella, Cronobacter, or pathogenic E. coli. For thermal processed food or ingredients, this provides an indication of the efficiency of inactivation treatment, but also as a prevention of recontamination. Testing of total EB is based on the standard ISO 21528-1 method, which is labor intensive and requires at least 2 days for the screening of negative samples.

The method is intended for the detection of Enterobacteriaceae in infant formula and related raw ingredients as well as environmental samples. The method is also a complement to our iQ-Check Cronobacter Solution.

Due to the sensitivity and specificity of PCR, results can be obtained in as little as 3 hr following a single enrichment in non-selective buffered peptone water (3564684 and 3554179).

Highly Specific and Reliable

iQ-Check Enterobacteriaceae Kit uses an optimized system of primers and probes to ensure high specificity. It is designed as a multiplex reaction that includes an internal inhibition control that is amplified in parallel with the target DNA for a reliable result (negative result validation). The iQ-Check Free DNA Removal Solution completes and optimizes the method by removing free DNA from food and environmental samples.

Maximum Flexibility

Developed as an open and flexible system, the iQ-Check Enterobacteriaceae Kit can be used for up to 94 samples. Reactions can be run on low- or high-throughput Bio-Rad instruments, depending on the laboratory’s needs. Different iQ-Check Assays can be run in parallel on the same plate using the iQ-Check Prep Automation System.

A Complete Solution

Bio-Rad offers a complete solution of standardized reagents, such as enrichment media, PCR kits, confirmation agars, PCR detection systems, and software for automated analysis specific for each test.

Kit Components

  • Lysis reagent for DNA extraction
  • Fluorescent probes
  • PCR amplification mix
  • Positive and negative PCR controls

Specifications

Specifications

Organism
Enterobacteriaceae

Ordering

Ordering

items

Use the filters below to refine results!

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12003068

Pkg of 1, kit includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples

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3594970

Pkg of 1, 1 vial of lyophilized iQ-Check Free DNA Removal Reagent (to make 10 ml) and 1 vial of iQ-Check 10x Activation Buffer (9 ml)

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3554179
Pkg of 6, 225 ml bottles — for challenging and non-challenging enrichment conditions
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3564684
Pkg of 1, 500 g — for challenging and non-challenging enrichment conditions

Accessories

Accessories

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HSP9655XTU
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, rigid 2-component design
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HSP9955XTU
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, barcoded, 7-digit sequential, Code 128, rigid 2-component design
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TLS0851XTU

Pkg of 120, thin-wall polypropylene 8–tube strips for PCR and low volume reactions, white, (960 tubes)

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TCS0803XTU
Pkg of 120, 8-cap strips for 0.2 ml tubes and plates for PCR and optical reactions, ultraclear (960 caps)
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MLL4851XTU
Pkg of 50, white PCR plate, 8 x 6 format, one-component polypropylene, can be cut for fewer samples
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MLL9651XTU
Pkg of 25, white PCR plate, one-component polypropylene, can be cut for fewer samples

Equipment

Equipment

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3600037

Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, CFX96 Deep Well Optical Reaction Module, cables. To be used with CFX Manager Software, Industrial Diagnostic Edition.

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17007992

96-well, 5-color plus FRET, network-connected real-time PCR detection system, includes CFX Opus 96 base unit, cables

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12016658
17007991

96-well, 5-color plus FRET, network-connected real-time PCR detection system, includes CFX Opus 96 base unit, and cables

Documents

Documents

Number Description Options
10000081305
iQ-CheckEnterobacteriaceae, User Guide (English, French, German, Italian, Portuguese, Spanish)
7449
Performance summary, iQ-Check Enterobacteriaceae
FSD_16439
iQ-Check Real-Time PCR Solution Brochure
3311
CFX Opus 96 Real-Time PCR System brochure
3309
CFX Opus 96 and CFX Opus Deepwell Real-Time PCR system product information sheet
10000126749
PIF Supplement, User Guide (English, French, German, Italian, Portuguese, Spanish, Chinese)
7381
PIF Supplement flyer
7023
iQ-Check Enterobacteriaceae Quick Guide
[ Add to Cart (Free) ]
3240
iQ-Check PCR Kits Easy I and II Extraction TUBE Protocol Quick Guide
FSD_17933
Food Safety and Quality Reference Guide
10000058391
iQ-Check Free DNA Removal Solution Product Insert
7322
iQ-Check Free DNA Removal Solution — Quick Guide
6834
iQ-Check Free DNA Removal Solution Brochure
10000119734
iQ-Check Purification Reagent Technical sheet
6960
iQ-Check Prep Solution Brochure
7274
Complete Solutions for Service Laboratories
10000128740
Buffered Peptone Water Plus, User Guide (English, French, German, Italian, Portuguese, Spanish, Chinese)
10000128738
Buffered Peptone Water Standard, User Guide (English, French, German, Italian, Portuguese, Spanish)
3276
CFX Manager Software (IDE) iQ-Check Prep sample setup Protocol, Quick Guide