Overview
Enterobacteriaceae (EB), often used as indicators for hygiene process criteria, is a large family of gram-negative bacteria that encompasses some of the most well-known food pathogens like Salmonella, Cronobacter, or pathogenic E. coli. For thermal processed food or ingredients, this provides an indication of the efficiency of inactivation treatment, but also as a prevention of recontamination. Testing of total EB is based on the standard ISO 21528-1 method, which is labor intensive and requires at least 2 days for the screening of negative samples.
The method is intended for the detection of Enterobacteriaceae in infant formula and related raw ingredients as well as environmental samples. The method is also a complement to our iQ-Check Cronobacter Solution.
Due to the sensitivity and specificity of PCR, results can be obtained in as little as 3 hr following a single enrichment in non-selective buffered peptone water (3564684 and 3554179).
Highly Specific and Reliable
iQ-Check Enterobacteriaceae Kit uses an optimized system of primers and probes to ensure high specificity. It is designed as a multiplex reaction that includes an internal inhibition control that is amplified in parallel with the target DNA for a reliable result (negative result validation). The iQ-Check Free DNA Removal Solution completes and optimizes the method by removing free DNA from food and environmental samples.
Maximum Flexibility
Developed as an open and flexible system, the iQ-Check Enterobacteriaceae Kit can be used for up to 94 samples. Reactions can be run on low- or high-throughput Bio-Rad instruments, depending on the laboratory’s needs. Different iQ-Check Assays can be run in parallel on the same plate using the iQ-Check Prep Automation System.
A Complete Solution
Bio-Rad offers a complete solution of standardized reagents, such as enrichment media, PCR kits, confirmation agars, PCR detection systems, and software for automated analysis specific for each test.
Kit Components
- Lysis reagent for DNA extraction
- Fluorescent probes
- PCR amplification mix
- Positive and negative PCR controls
Specifications
Ordering
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Pkg of 1, kit includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples
Pkg of 1, 1 vial of lyophilized iQ-Check Free DNA Removal Reagent (to make 10 ml) and 1 vial of iQ-Check 10x Activation Buffer (9 ml)
Pkg of 1, vial of Purification Reagent (to make 25 ml), for 96 tests
Pkg of 1, 2 g vial
Pkg of 6, 225 ml bottles — for challenging and non-challenging enrichment conditions
Pkg of 1, 500 g — for challenging and non-challenging enrichment conditions
Accessories
Pkg of 100, optically clear seal for PCR plates for real-time PCR
Pkg of 500, screwcap micro test tube, sterilized, with installed O-ring screwcaps
Pkg of 960, 20–200 µl pipet tip with aerosol barrier, racked, certified free of pyrogen, DNase, RNase; For food application only
Pkg of 1,000, 100–1,000 µl pipet tip with aerosol barrier, bulk, certified free of pyrogen, DNas
Pkg of 960, 100–1,000 µl pipet tip with aerosol barrier, racked, certified free of pyrogen, DNase, RNase, For food application only
Pkg of 960, 2–20 µl pipet tip with aerosol barrier, racked, certified free of pyrogen, DNase, RNase; For food application only
Equipment
Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, CFX96 Deep Well Optical Reaction Module, cables. To be used with CFX Manager Software, Industrial Diagnostic Edition.
96-well, 5-color plus FRET, network-connected real-time PCR detection system, includes CFX Opus 96 base unit, cables
96-well, 5-color plus FRET, network-connected real-time PCR detection system, includes CFX Opus 96 base unit, and cables
