iQ-Check Enterobacteriaceae PCR Detection Kit

iQ-Check Enterobacteriaceae PCR Detection Kit

Complete sample preparation and PCR kit for the detection of Enterobacteriaceae in food and environmental samples. Next day results for all samples.

Read More
Ordering Information

Overview

Consult with a Specialist

Enterobacteriaceae (EB), often used as indicators for hygiene process criteria, is a large family of gram-negative bacteria that encompasses some of the most well-known food pathogens like Salmonella, Cronobacter, or pathogenic E. coli. For thermal processed food or ingredients, this provides an indication of the efficiency of inactivation treatment, but also as a prevention of recontamination. Testing of total EB is based on the standard ISO 21528-1 method, which is labor intensive and requires at least 2 days for the screening of negative samples.

The method is intended for the detection of Enterobacteriaceae in infant formula and related raw ingredients as well as environmental samples. The method is also a complement to our iQ-Check Cronobacter Solution.

Due to the sensitivity and specificity of PCR, results can be obtained in as little as 3 hr following a single enrichment in non-selective buffered peptone water (3564684 and 3554179).

Highly Specific and Reliable

iQ-Check Enterobacteriaceae Kit uses an optimized system of primers and probes to ensure high specificity. It is designed as a multiplex reaction that includes an internal inhibition control that is amplified in parallel with the target DNA for a reliable result (negative result validation). The iQ-Check Free DNA Removal Solution completes and optimizes the method by removing free DNA from food and environmental samples.

Maximum Flexibility

Developed as an open and flexible system, the iQ-Check Enterobacteriaceae Kit can be used for up to 94 samples. Reactions can be run on low- or high-throughput Bio-Rad instruments, depending on the laboratory’s needs. Different iQ-Check Assays can be run in parallel on the same plate using the iQ-Check Prep Automation System.

A Complete Solution

Bio-Rad offers a complete solution of standardized reagents, such as enrichment media, PCR kits, confirmation agars, PCR detection systems, and software for automated analysis specific for each test.

Kit Components

  • Lysis reagent for DNA extraction
  • Fluorescent probes
  • PCR amplification mix
  • Positive and negative PCR controls

Specifications

Organism
Enterobacteriaceae

Ordering

items

Use the filters below to refine results!

Image
iq-check-enterobacteriaceae-kit-12003068-th.png
12003068

Pkg of 1, kit includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples

Image
iQ-Check-Free-DNA-Removal-16-0708-thumb.jpg
3594970

Pkg of 1, 1 vial of lyophilized iQ-Check Free DNA Removal Reagent (to make 10 ml) and 1 vial of iQ-Check 10x Activation Buffer (9 ml)

Image
355-4179_160px.jpg
3554179

Pkg of 6, 225 ml bottles — for challenging and non-challenging enrichment conditions

Image
356-4684_160.jpg
3564684

Pkg of 1, 500 g — for challenging and non-challenging enrichment conditions

Accessories

Image
HSP9655_tn.jpg
HSP9655XTU
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, rigid 2-component design
Image
HSP9955_tn.jpg
HSP9955XTU
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, barcoded, 7-digit sequential, Code 128, rigid 2-component design
Image
TCS-0803_tn.jpg
TCS0803XTU
Pkg of 120, 8-cap strips for 0.2 ml tubes and plates for PCR and optical reactions, ultraclear (960 caps)
Image
mll-4851_tn.jpg
MLL4851XTU
Pkg of 50, white PCR plate, 8 x 6 format, one-component polypropylene, can be cut for fewer samples
Image
mll-9651_tn.jpg
MLL9651XTU
Pkg of 25, white PCR plate, one-component polypropylene, can be cut for fewer samples
Image
17010688
17010688

Pkg of 960, 20–200 µl pipet tip with aerosol barrier, racked, certified free of pyrogen, DNase, RNase; For food application only

Image
17010624
2112036XTU

Pkg of 1,000, 100–1,000 µl pipet tip with aerosol barrier, bulk, certified free of pyrogen, DNas

Image
17010692
2112021XTU

Pkg of 960, 100–1,000 µl pipet tip with aerosol barrier, racked, certified free of pyrogen, DNase, RNase, For food application only

Image
17010691
2112006XTU

Pkg of 960, 2–20 µl pipet tip with aerosol barrier, racked, certified free of pyrogen, DNase, RNase; For food application only

Equipment

Image
185-5096_tn.jpg
3600037

Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, CFX96 Deep Well Optical Reaction Module, cables. To be used with CFX Manager Software, Industrial Diagnostic Edition.

Image
12011319_tn.jpg
17007992

96-well, 5-color plus FRET, network-connected real-time PCR detection system, includes CFX Opus 96 base unit, cables

Image
12016658
17007991

96-well, 5-color plus FRET, network-connected real-time PCR detection system, includes CFX Opus 96 base unit, and cables

Documents

Number Description
Add to Cart (Free)