iQ-Check Enterobacteriaceae PCR Detection Kit

Overview

Complete sample preparation and PCR kit for the detection of Enterobacteriaceae in food and environmental samples. Next day results for all samples.

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Accessories
Equipment
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Description

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Enterobacteriaceae (EB), often used as indicators for hygiene process criteria, is a large family of gram-negative bacteria that encompasses some of the most well-known food pathogens like Salmonella, Cronobacter, or pathogenic E. coli. For thermal processed food or ingredients, this provides an indication of the efficiency of inactivation treatment, but also as a prevention of recontamination. Testing of total EB is based on the standard ISO 21528-1 method, which is labor intensive and requires at least 2 days for the screening of negative samples.

The method is intended for the detection of Enterobacteriaceae in infant formula and related raw ingredients as well as environmental samples. The method is also a complement to our iQ-Check Cronobacter Solution.

Due to the sensitivity and specificity of PCR, results can be obtained in as little as 3 hr following a single enrichment in non-selective buffered peptone water (3564684 and 3554179).

Highly Specific and Reliable

iQ-Check Enterobacteriaceae Kit uses an optimized system of primers and probes to ensure high specificity. It is designed as a multiplex reaction that includes an internal inhibition control that is amplified in parallel with the target DNA for a reliable result (negative result validation). The iQ-Check Free DNA Removal Solution completes and optimizes the method by removing free DNA from food and environmental samples.

Maximum Flexibility

Developed as an open and flexible system, the iQ-Check Enterobacteriaceae Kit can be used for up to 94 samples. Reactions can be run on low- or high-throughput Bio-Rad instruments, depending on the laboratory’s needs. Different iQ-Check Assays can be run in parallel on the same plate using the iQ-Check Prep Automation System.

A Complete Solution

Bio-Rad offers a complete solution of standardized reagents, such as enrichment media, PCR kits, confirmation agars, PCR detection systems, and software for automated analysis specific for each test.

Kit Components

Lysis reagent for DNA extraction
Fluorescent probes
PCR amplification mix
Positive and negative PCR controls

Related Products

Specifications

Organism

Enterobacteriaceae

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(Discontinued) iQ-Check Enterobacteriaceae PCR Detection Kit

12003068

Pkg of 1, kit includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples

(Discontinued) iQ-Check Free DNA Removal Solution

3594970

Pkg of 1, 1 vial of lyophilized iQ-Check Free DNA Removal Reagent (to make 10 ml) and 1 vial of iQ-Check 10x Activation Buffer (9 ml)

(Discontinued) iQ-Check Purification Reagent

12012383

Pkg of 1, vial of Purification Reagent (to make 25 ml), for 96 tests

(Discontinued) PIF Supplement

12013322

Pkg of 1, 2 g vial

(Discontinued) RAPID'Enterobacteriaceae Agar, ready to use

3554012

Pkg of 6, 200 ml bottles

(Discontinued) RAPID'Enterobacteriaceae Agar, dehydrated

3564004

Pkg of 1, 500 g of dehydrated agar broth, enough for 13.5 L of medium

(Discontinued) VRBG (Violet Red Bile Glucose)/Agar

3564584

500 g

(Discontinued) Buffered Peptone Water Plus

3554179

Pkg of 6, 225 ml bottles — for challenging and non-challenging enrichment conditions

(Discontinued) VRBG (Violet Red Bile Glucose)/Agar

3554239

100 ml x 6 bottles

(Discontinued) Buffered Peptone Water Plus

3555790

Pkg of 2, 5 L bags — for challenging and non-challenging enrichment conditions

(Discontinued) Buffered Peptone Water Plus

3555795

Pkg of 4, 3 L bags — for challenging and non-challenging enrichment conditions

(Discontinued) Buffered Peptone Water Plus

3564684

Pkg of 1, 500 g — for challenging and non-challenging enrichment conditions

(Discontinued) Buffered Peptone Water Plus

3564686

Pkg of 1, 5 kg — for challenging and non-challenging enrichment conditions

(Discontinued) Buffered Peptone Water Standard

12013260

Pkg of 5 L x 2 bags — for standard enrichment conditions

(Discontinued) Buffered Peptone Water Standard, dehydrated

12013259

Pkg of 1, 500 g — for standard enrichment conditions

(Discontinued) Buffered Peptone Water Standard, dehydrated

12013258

Pkg of 1, 5 kg — for standard enrichment conditions

Accessories

(Discontinued) Strip Cap Tool for PCR Plates and PCR Tubes

ECT2000

Pkg of 1, tool for seating 8- and 12-cap strips on PCR plates or tubes

(Discontinued) iQ-Check Deep Well Microplates

3594900

Pkg of 50, 96-well plates plates, deep well

(Discontinued) Hard-Shell 96-Well PCR Plates, low profile, thin wall, skirted, white/white

HSP9655XTU

Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, rigid 2-component design

(Discontinued) Hard-Shell 96-Well PCR Plates, low profile, thin wall, skirted, white/white, barcoded

HSP9955XTU

Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, barcoded, 7-digit sequential, Code 128, rigid 2-component design

(Discontinued) Microseal 'B' PCR Plate Sealing Film, adhesive, optical

MSB1001XTU

Pkg of 100, optically clear seal for PCR plates for real-time PCR

(Discontinued) Pre-Pierced Plate Sealing Film

3600040

Pkg of 100, PCR plate sealing film, pre-pierced

(Discontinued) X-Pierce Films

3593977

Pkg of 100, PCR plate sealing film, pre-pierced

(Discontinued) 1.5 ml Conical Tubes, screwcap, sterile

2240110XTU

Pkg of 500, screwcap micro test tube, sterilized, with installed O-ring screwcaps

(Discontinued) PCR Tube 8-Cap Strips, optical, flat, 8 caps

TCS0803XTU

Pkg of 120, 8-cap strips for 0.2 ml tubes and plates for PCR and optical reactions, ultraclear (960 caps)

(Discontinued) Multiplate™ 48-Well PCR Plates, low-profile, unskirted, white

MLL4851XTU

Pkg of 50, white PCR plate, 8 x 6 format, one-component polypropylene, can be cut for fewer samples

(Discontinued) Multiplate 96-Well PCR Plates, low-profile, unskirted

MLL9651XTU

Pkg of 25, white PCR plate, one-component polypropylene, can be cut for fewer samples

Equipment

(Discontinued) CFX96 Touch Deep Well Real-Time PCR Detection System

3600037

Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, CFX96 Deep Well Optical Reaction Module, cables. To be used with CFX Manager Software, Industrial Diagnostic Edition.