iQ-Check Salmonella II PCR Detection Kit

Image
iQ_Check_Salmonella_II_Kit 2019.jpg

Overview

Complete kit including extraction and amplification reagents for the rapid detection of Salmonella spp in food, animal feed, and environmental samples. 12 hr to results for raw meat samples, next day for all other samples. 

Read full description Ordering Information

Description

Description

Talk with a Specialist

The iQ-Check Salmonella II PCR Detection Kit is based on the fast, sensitive, and proven technology of real-time PCR. The kit is intended for detecting Salmonella spp. in animal feed and environmental samples, as well as in food samples such as poultry, eggs, and raw meats.

Due to the sensitivity and specificity of PCR, results can be obtained in little as 12 hr for raw meat samples and in 20 hr for all other samples following a single enrichment in a nonselective medium.

Highly Specific and Reliable

The iQ-Check Salmonella II Kit uses an optimized system of primers and probes to ensure high specificity and eliminate cross-reactions. It is designed as a multiplex reaction that includes an internal inhibition control that is amplified in parallel with the target DNA for a reliable result.

Maximum Flexibility

Developed as an open and flexible system, the iQ-Check Salmonella II Kit can be used for up to 94 samples. Reactions can be run on low- or high-throughput Bio-Rad instruments, depending on the laboratory’s needs. Different iQ-Check assays can be run in parallel on the same plate.

Validated Protocols

The iQ-Check Salmonella II protocol has been validated by several certification bodies. These validation studies have shown that the kit is equivalent to or better than the various reference methods evaluated and provides results in much less time.

A Complete Solution

Bio-Rad offers a complete solution with standardized reagents, such as enrichment media, PCR kits, confirmation agars, and PCR detection systems, in addition to software for automated analysis specific to each test.

Kit Components

  • Lysis reagent for DNA extraction
  • Fluorescent probes
  • PCR amplification mix
  • Positive and negative PCR controls

Related Products

Specifications

Specifications

Ordering

Ordering

items

Use the filters below to refine results!

iQ_Check_Salmonella_II_Kit_2019_SKU_TN.jpg
3578123
Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 reactions
iQ-Check-Free-DNA-Removal-16-0708-thumb.jpg
3594970
Pkg of 1, 1 vial of lyophilized iQ-Check Free DNA Removal Reagent (to make 10 ml) and 1 vial of iQ-Check 10x Activation Buffer (9 ml)
12012383_sku_TN.jpg
12012383
Pkg of 1, vial of Purification Reagent (to make 25 ml), for 96 tests
12013322_sku_tn.jpg
(Discontinued) PIF Supplement
12013322

Pkg of 1, 2 g vial

3564709
Pkg of 100 capsules, each enough for 2.5 L enrichment broth for 250 g sample
3564712
Pkg of 1, enough for 100 analyses
3564710
Pkg of 100 capsules, each enough for 250 ml enrichment broth for 25 g sample
3555790_th.jpg
3555790
Pkg of 2, 5 L bags — for challenging and non-challenging enrichment conditions
355-4179_160px.jpg
3554179
Pkg of 6, 225 ml bottles — for challenging and non-challenging enrichment conditions
3555795_th.jpg
3555795
Pkg of 4, 3 L bags — for challenging and non-challenging enrichment conditions
356-4684_160.jpg
3564684
Pkg of 1, 500 g — for challenging and non-challenging enrichment conditions
3564686_th.jpg
3564686
Pkg of 1, 5 kg — for challenging and non-challenging enrichment conditions
3555795_th.jpg
12013260
Pkg of 5 L x 2 bags — for standard enrichment conditions
12013259_th.jpg
12013259
Pkg of 1, 500 g — for standard enrichment conditions
12013258_th.jpg
12013258
Pkg of 1, 5 kg — for standard enrichment conditions

Accessories

Accessories

360-0040_160.jpg
3600040
Pkg of 100, PCR plate sealing film, pre-pierced
msb-1001_tn.jpg
MSB1001XTU
Pkg of 100, optically clear seal for PCR plates for real-time PCR
224-0110_160px.jpg
2240110XTU
Pkg of 500, screwcap micro test tube, sterilized, with installed O-ring screwcaps
tls-0851_tn.jpg
TLS0851XTU
Pkg of 120, thin-wall polypropylene 8–tube strips for PCR and low volume reactions, white, (960 tubes)
TCS-0803_tn.jpg
TCS0803XTU
Pkg of 120, 8-cap strips for 0.2 ml tubes and plates for PCR and optical reactions, ultraclear (960 caps)
mll-4851_tn.jpg
MLL4851XTU
Pkg of 50, white PCR plate, 8 x 6 format, one-component polypropylene, can be cut for fewer samples
mll-9651_tn.jpg
MLL9651XTU
Pkg of 25, white PCR plate, one-component polypropylene, can be cut for fewer samples
ect-2000_tn.jpg
ECT2000
Pkg of 1, tool for seating 8- and 12-cap strips on PCR plates or tubes
HSP9655_tn.jpg
HSP9655XTU
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, rigid 2-component design
HSP9955_tn.jpg
HSP9955XTU
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, barcoded, 7-digit sequential, Code 128, rigid 2-component design
3594900_tn.jpg
3594900
Pkg of 50, 96-well plates plates, deep well
msb-1001_tn.jpg
MSB1001
Pkg of 100, optically clear seal for PCR plates
3593977_tn.jpg
(Discontinued) X-Pierce Films
3593977
Pkg of 100, PCR plate sealing film, pre-pierced

Equipment

Equipment

185-5096_tn.jpg
3600037

Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, CFX96 Deep Well Optical Reaction Module, cables. To be used with CFX Manager Software, Industrial Diagnostic Edition.

359-4911_iQ-check-prep_tn.jpg
(Discontinued) iQ-Check Prep System
3594911
Automated system for DNA extraction and real-time PCR plate set-up, 1 unit
thermoshaker-359-4999-thumb.jpg
(Discontinued) Heating Thermoshaker
3594995
Pkg of 1, temperature-controlled shaker for cell lysis, for use with iQ-Check PCR Detection Kits
3594996
Pkg of 1, deep well block for thermoshaker
359-3893_CFXmanager_160.jpg

Documents

Documents

Number Description Options
10000131519
iQ-Check Salmonella spp. II, User Guide (English, French, German, Italian, Portuguese, Spanish)
FSD_16439
iQ-Check Real-Time PCR Solution Brochure
7426
Performance Summary, iQ-Check Salmonella II
6960
iQ-Check Prep Solution Brochure
10000126749
PIF Supplement, User Guide (English, French, German, Italian, Portuguese, Spanish)
7381
PIF Supplement flyer
7039
Easy I Extraction Deep Well Protocol Using the Thermoshaker Quick Guide
3240
iQ-Check PCR Kits Easy I and II Extraction TUBE Protocol Quick Guide
FSD_17933
Food Safety and Quality Reference Guide
10000058391
iQ-Check Free DNA Removal Solution Product Insert
7322
iQ-Check Free DNA Removal Solution — Quick Guide
6834
iQ-Check Free DNA Removal Solution Brochure
10000119734
iQ-Check Purification Reagent Technical sheet
PF_175
iQ-Check Salmonella II Kit Quick Guide: Easy II Extraction – Meat Products
TN_Salmonella_Sponges
Analysis of Pooled Environmental Sponges Using the iQ-Check Salmonella spp. Real‑Time PCR Kit
TN_Salmonella_Spice
Internal Comparative Evaluation Study of Alternative Enrichment Protocols for Detection of Salmonella in Untreated Spices, Ver A
7274
Complete Solutions for Service Laboratories
10000128740
Buffered Peptone Water Plus, User Guide (English, French, German, Italian, Portuguese, Spanish)
10000128738
Buffered Peptone Water Standard, User Guide (English, French, German, Italian, Portuguese, Spanish)