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iScript cDNA Synthesis Kits | iScript Explore Kit | Resources | Applications & Technologies | Related Products |
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iScript Reverse Transcription Reagents feature an RNase H+ MMLV reverse transcriptase plus an RNase inhibitor for reliable yields. Choose the best cDNA synthesis kit for your application, with formulations optimized for ease, yield, flexibility, fidelity, or gDNA clearance.
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iScript cDNA Synthesis Kits
![]() iScript Reverse Transcription Supermix for RT-qPCR |
![]() iScript gDNA Clear cDNA Synthesis Kit |
![]() iScript Advanced cDNA Synthesis Kit for RT-qPCR |
![]() iScript Select cDNA Synthesis Kit |
![]() iScript cDNA Synthesis Kit |
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Features |
Minimum reaction
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Effective gDNA removal before reverse transcription |
Maximum RNA input for high cDNA yields |
Flexible priming options and high-fidelity |
Reliable value solution |
Applications |
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Total Input RNA Range | 1 µg–1 pg | 1 µg–1 pg | 7.5 µg–100 fg | 1 µg–1 pg | 1 µg–100 fg |
Kit Format | 1 tube | 3 tubes | 2 tubes | 5 tubes | 2 tubes |
Kit Contents |
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Time to Produce cDNA | 26 min | 36 min | 21 min | 15–65 min | 26 min |
RNase H+ Activity | ✓ | ✓ | ✓ | ✓ | ✓ |
Documents | Manual |
iScript Explore One-Step RT and PreAmp Kit
- Reverse transcription and unbiased preamplification completed in a single step
- Highly sensitive target detection
- Effective genomic DNA clearance
Resources

Understanding Reverse Transcription
This interactive tutorial provides an introduction to reverse transcription basics, an explanation of how different reagents work, and a protocol for comparing the results with different reverse transcription reagents.

Reagent Comparison Guide for Real-Time PCR
(PDF 3 MB)
Amplification Reagents and Plastics Brochure
(PDF 6.1 MB)
iScript Reverse Transcription Kits Flier
(PDF 208 KB)
Videos
Applications & Technologies
qPCR/Real-Time PCR Reagents
Guidelines for selecting the appropriate reagents for your real-time PCR-based application
What is Gene Expression Analysis?
An introduction to gene regulation mechanisms, methods for gene expression profiling, and quantitation techniques including real-time PCR
lncRNA RT-qPCR Workflow
This innovative workflow enables highly sensitive detection of lncRNAs and helps to overcome challenges associated with lncRNA expression analysis
Real-Time PCR Doctor
Having trouble with real-time PCR experiments? The Real-Time PCR Doctor can help you diagnose and correct common qPCR problems
Related Products
Real-Time PCR Supermixes and Kits
PCR and real-time PCR reagents with advanced enzyme engineering and formulations optimized for a wide variety of templates and target sequences
One-Step RT-qPCR from RNA or Cells
For robust reverse transcription and SYBR Green or probe-based qPCR in one step on any instrument
Real-Time PCR Detection Systems
An automation-compatible, gradient-enabled thermal cycler with interchangeable optical reaction modules for singleplex or multiplex real-time PCR
Cell Lysis RT-qPCR Kits
Complete and fast solution for one- or two-step qPCR reactions directly from cell lysates with no RNA purification step
Thermal Cyclers for PCR
Personal units and flexible modular thermal cyclers provide low- to high-throughput capabilities for all your PCR amplification needs
Preamplification Supermix
Ready-to-use supermix for unbiased, target-specific preamplification of cDNA or gDNA using up to 100 qPCR assays
PCR Plastic Consumables
A large selection of thin-wall polypropylene PCR tubes, PCR plates, seals, and accessories precisely manufactured for various PCR applications