
iScript cDNA Synthesis Kits
iScript Reverse Transcription Reagents feature an RNase H+ MMLV reverse transcriptase plus an RNase inhibitor for reliable yields. Choose the best cDNA synthesis kit for your application, with formulations optimized for ease, yield, flexibility, fidelity, or gDNA clearance.
iScript cDNA Synthesis Kits
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![]() iScript Reverse Transcription Supermix for RT-qPCR |
![]() iScript gDNA Clear cDNA Synthesis Kit |
![]() iScript Advanced cDNA Synthesis Kit for RT-qPCR |
![]() iScript Select cDNA Synthesis Kit |
![]() iScript cDNA Synthesis Kit |
Features |
Minimum reaction
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Effective gDNA removal before reverse transcription |
Maximum RNA input for high cDNA yields |
Flexible priming options and high-fidelity |
Reliable value solution |
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Applications |
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1 µg–1 pg | 1 µg–1 pg | 7.5 µg–100 fg | 1 µg–1 pg | 1 µg–100 fg | |
Kit Format | 1 tube | 3 tubes | 2 tubes | 5 tubes | 2 tubes |
Kit Contents |
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26 min | 36 min | 21 min | 15–65 min | 26 min | |
RNase H+ Activity | ✓ | ✓ | ✓ | ✓ | ✓ |
Documents | Manual |

CFX Loyalty Program
Earn a free one-year service contract on your CFX System with purchase of Reagents
iScript Explore Kit

iScript Explore One-Step RT & PreAmp Kit
- Reverse transcription and unbiased preamplification completed in a single step
- Highly sensitive target detection
- Effective genomic DNA clearance
Resources

Understanding Reverse Transcription
This interactive tutorial provides an introduction to reverse transcription basics, an explanation of how different reagents work, and a protocol for comparing the results with different reverse transcription reagents.
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Reagent Comparison Guide for Real-Time PCR
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Amplification Reagents & Plastics Brochure
(PDF 6.1 MB) -
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iScript Reverse Transcription Kits Flier
Videos
Applications & Technologies
qPCR/Real-Time PCR Reagents
Guidelines for selecting the appropriate reagents for your real-time PCR-based application
What is Gene Expression Analysis?
An introduction to gene regulation mechanisms, methods for gene expression profiling, and quantitation techniques including real-time PCR
lncRNA RT-qPCR Workflow
This innovative workflow enables highly sensitive detection of lncRNAs and helps to overcome challenges associated with lncRNA expression analysis
Real-Time PCR Doctor
Having trouble with real-time PCR experiments? The Real-Time PCR Doctor can help you diagnose and correct common qPCR problems