
Description
Description
Use Tris Buffered Saline (TBS) with 1% (w/v) Casein for blocking membranes for blotting. Blocking buffers containing casein can provide lower backgrounds than buffers containing low fat milk, and casein is recommended for applications using biotin-avidin complexes. Tris Buffered Saline (TBS) with 1% (w/v) Casein is recommended for applications using alkaline phosphate since unlike phosphate-containing buffers it does not interfere with enzyme activity.
Related Products
- PrecisionAb™ Validated Western Blotting Antibodies
- Criterion™ Staining/Blotting Trays with Lids (3459921)
- 10x Tris Buffered Saline (TBS) (1706435)
- 10x Phosphate Buffered Saline (PBS) (1610780)
- 1x Phosphate Buffered Saline (PBS) with 1% Casein (1610783)
Related Categories
- Protein Gels
- Western Blotting Membranes and Filter Paper
- Semi-Dry and Rapid Blotting Systems
- Wet/Tank Blotting Systems
- Power Supplies
- Transfer Buffers
- Immunodetection Reagents and Kits
- Gel and Western Imaging Systems
Supporting Documents
- Mini-PROTEAN® Precast Gels Instruction Manual and Application Guide
- Criterion Gel Application Guide Instruction Manual
- Mini Trans-Blot® Cell Instruction Manual
- Trans-Blot SD Cell Quick Reference Guide
- Trans-Blot SD Semi-Dry Transfer Cell Instruction Manual