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TGX FastCast Acrylamide Solutions

TGX FastCast Acrylamide Solutions

TGX FastCast acrylamide kits for hand casting polyacrylamide gels enable fast, easy casting and produce long-life gels. Gels made from FastCast kits have shorter run times and higher transfer efficiency.

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Overview

TGX FastCast premixed acrylamide solutions bring all the benefits of TGX gel chemistry to handcast polyacrylamide gels. TGX (Tris-Glycine eXtended) chemistry retains Laemmli-like separation characteristics while using a standard Tris-glycine running buffer system. Protein samples in the gel can be separated in only 20 minutes. TGX FastCast premixed acrylamide solutions for hand casting are available in the most commonly used cross-linking ratios for PAGE systems.

Features and Benefits of TGX FastCast Acrylamide Solutions

  • Handcast gels ready to run in as little as 30 min
  • Long shelf life — handcast gels remain fresh for up to 4 weeks at 4°C
  • Electrophoresis run times as short as 20 min using Mini-PROTEAN Tetra or Criterion Cells
  • Efficient protein transfers in as little as 3 min using the Trans-Blot Turbo Transfer System

Applications and Uses of TGX FastCast Acrylamide Solutions

  • 1-D polyacrylamide gel electrophoresis for protein separation
  • Accurate molecular weight estimation
  • Robust separation for complex samples

Approximate number of gels per kit.

Number of Gels Included in Mini and Midi Gel Kits
Mini Gels (~9 x 7 cm) Midi Gels (~13 x 9 cm)
Mini-PROTEAN
Cassettes 		0.75 mm
Glass Plates 		1.0 mm
Glass Plates 		1.5 mm
Glass Plates 		Criterion
Cassettes
Starter Kit 11 gels 19 gels 13 gels 9 gels 7 gels
Full Kit 55 gels 75 gels 65 gels 45 gels 35 gels

More Information

TGX FastCast tutorial video

Watch the TGX FastCast Tutorial Video to see how quick and easy it is to cast gels with TGX FastCast Acrylamide Kits. View step-by-step instructions for casting mini gels using the Mini-PROTEAN Tetra Casting Module.

Bio-Rad Gel Electrophoresis Systems

Gel handcasting equipment and electrophoresis cells are offered in mini and midi gel formats. See the electrophoresis system selection guide for more details.

Mini gel systems:

Preconfigured Mini-PROTEAN Tetra Cells can be ordered with the following features:

4-gel cell with 2 casting stands, 4 casting frames, 10-well combs, 5 short plates, and 5 spacer plates

See the Tetra cell bundle selection guide for bundles. To configure your own electrophoresis cell, order the four-gel Mini-PROTEAN Tetra Cell (1658004) and select a casting module from the Accessories tab.

Midi gel systems:

Criterion and Criterion Dodeca Cells use integral cassettes for gel hand casting. 1.0 mm thick gel casting sets are available in various comb configurations:

Related Technologies

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(Discontinued) TGX™ FastCast™ Acrylamide Starter Kit, 7.5%
1610170
Starter kit for hand casting 7.5% TGX polyacrylamide gels, includes acrylamide solutions and buffers
(Discontinued) TGX™ FastCast™ Acrylamide Kit, 7.5%
1610171

Kit for hand casting 7.5% TGX polyacrylamide gels, includes acrylamide solutions and buffers

(Discontinued) TGX™ FastCast™ Acrylamide Starter Kit, 10%
1610172
Starter kit for hand casting 10% TGX polyacrylamide gels, includes acrylamide solutions and buffers
(Discontinued) TGX™ FastCast™ Acrylamide Starter Kit, 12%
1610174
Starter kit for hand casting 12% TGX polyacrylamide gels, includes acrylamide solutions and buffers

Accessories

(Discontinued) Mini-PROTEAN® Tetra Vertical Electrophoresis Cell, 4-gel, for 1.0 mm thick handcast gels
1658001FC
4-gel vertical electrophoresis system, 1.0 mm gel thickness, includes companion module, 2 casting stands, 4 casting frames, 10-well combs, 5 short plates, and 5 spacer plates, with TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10% (161-0182)
(Discontinued) Mini-PROTEAN® Tetra Vertical Electrophoresis Cell, 4-gel, for 1.5 mm thick handcast gels
1658006FC
4-gel vertical electrophoresis system, 1.5 mm gel thickness, includes companion module, 2 casting stands, 4 casting frames, 10-well combs, 5 short plates, and 5 spacer plates, with TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10% (161-0182)

Kits & Reagents

(Discontinued) 10x Tris/Glycine/SDS
1610772
Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water

Standards

(Discontinued) Precision Plus Protein™ Dual Color Standards, 2.5 ml
1610394
2.5 ml (5 x 500 µl), mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 250 applications
(Discontinued) Precision Plus Protein™ Kaleidoscope™ Prestained Protein Standards, value pack
1610395

2.5 ml (5 x 500 µl), mixture of ten multicolor recombinant proteins (10–250 kD), 250 applications

(Discontinued) Precision Plus Protein™ Dual Xtra Prestained Protein Standards, value pack
1610397
2.5 ml (5 x 500 µl), mixture of 12 recombinant proteins (2–250 kD), nine blue-stained bands, and three pink reference bands (2, 25, 75 kD), 250 applications
(Discontinued) Precision Plus Protein™ Unstained Protein Standards, Strep-tagged, recombinant, 5 ml
1610396
5 ml (5 x 1 ml), unstained mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 500 applications

Plates

(Discontinued) Mini-PROTEAN® Spacer Plates with 0.75 mm Integrated Spacers
1653310

Pkg of 5, spacer plate with 0.75 mm integrated spacer, for Mini-PROTEAN Tetra System and Mini-PROTEAN 3 Multi-Casting Chamber

(Discontinued) Mini-PROTEAN® Spacer Plates with 1.0 mm Integrated Spacers
1653311

Pkg of 5, spacer plate with 1.0 mm integrated spacer, for Mini-PROTEAN Tetra System and Mini-PROTEAN 3 Multi-Casting Chamber

(Discontinued) Mini-PROTEAN® Spacer Plates with 1.5 mm Integrated Spacers
1653312

Pkg of 5, spacer plate with 1.5 mm integrated spacer, for Mini-PROTEAN Tetra System and Mini-PROTEAN 3 Multi-Casting Chamber

(Discontinued) Mini-PROTEAN® Empty Cassettes
4560003
Pkg of 50, cassettes with 10 well marks for casting gels 8.6 x 7.2 cm by 1.0 mm thick, for use with Mini-PROTEAN Electrophoresis Cells; purchase combs separately (456-0013)
(Discontinued) Mini-PROTEAN® Empty Cassettes
4560005
Pkg of 50, cassettes with 12 well marks for casting gels 8.6 x 7.2 cm by 1.0 mm thick, for use with Mini-PROTEAN Electrophoresis Cells; purchase combs separately (456-0015)

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