TGX FastCast Acrylamide Solutions

TGX FastCast acrylamide kits for hand casting polyacrylamide gels enable fast, easy casting and produce long-life gels. Gels made from FastCast kits have shorter run times and higher transfer efficiency.

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Overview

TGX FastCast premixed acrylamide solutions bring all the benefits of TGX gel chemistry to handcast polyacrylamide gels. TGX (Tris-Glycine eXtended) chemistry retains Laemmli-like separation characteristics while using a standard Tris-glycine running buffer system. Protein samples in the gel can be separated in only 20 minutes. TGX FastCast premixed acrylamide solutions for hand casting are available in the most commonly used cross-linking ratios for PAGE systems.

Features and Benefits of TGX FastCast Acrylamide Solutions

Handcast gels ready to run in as little as 30 min
Long shelf life — handcast gels remain fresh for up to 4 weeks at 4°C
Electrophoresis run times as short as 20 min using Mini-PROTEAN Tetra or Criterion Cells
Efficient protein transfers in as little as 3 min using the Trans-Blot Turbo Transfer System

Applications and Uses of TGX FastCast Acrylamide Solutions

1-D polyacrylamide gel electrophoresis for protein separation
Accurate molecular weight estimation
Robust separation for complex samples

Approximate number of gels per kit.

	Mini Gels (~9 x 7 cm)				Midi Gels (~13 x 9 cm)
		Mini-PROTEAN Cassettes	0.75 mm Glass Plates	1.0 mm Glass Plates	1.5 mm Glass Plates	Criteron Cassettes
Starter Kit	11 gels	19 gels	13 gels	9 gels	7 gels
Full Kit	55 gels	95 gels	65 gels	45 gels	35 gels

More Information

Watch the TGX FastCast Tutorial Video to see how quick and easy it is to cast gels with TGX FastCast Acrylamide Kits. View step-by-step instructions for casting mini gels using the Mini-PROTEAN Tetra Casting Module.

Learn how to cast better gels with Bio-Rad’s TGX and TGX Stain-Free FastCast Acrylamide Kits: Tips & Tricks guide.
Download the FastCast Kits calculator to automatically calculate the volumes of stacker, resolver, APS, and TEMED required when casting TGX FastCast and TGX Stain-Free FastCast Gels.

Bio-Rad Gel Electrophoresis Systems

Gel handcasting equipment and electrophoresis cells are offered in mini and midi gel formats. See the electrophoresis system selection guide for more details.

Mini gel systems:

Mini-PROTEAN Tetra and Mini-PROTEAN 3 Dodeca Cells with gel handcasting stands and frames
Individual handcasting accessories for the Mini-PROTEAN Tetra Handcast System
Mini-PROTEAN 3 multi-casting chamber to cast up to 12 gels

Preconfigured Mini-PROTEAN Tetra Cells can be ordered with the following features:

4-gel cell with 2 casting stands, 4 casting frames, 10-well combs, 5 short plates, and 5 spacer plates

0.75 mm spacer plates (1658000)
1.0 mm spacer plates (1658001)
1.5 mm spacer plates (1658006)

See the Tetra cell bundle selection guide for bundles. To configure your own electrophoresis cell, order the four-gel Mini-PROTEAN Tetra Cell (1658004) and select a casting module from the Accessories tab.

Midi gel systems:

Criterion and Criterion Dodeca Cells use integral cassettes for gel hand casting. 1.0 mm thick gel casting sets are available in various comb configurations:

12+2 well comb (3459901)
18-well comb (3459902)
26-well comb (3459903)

Related Technologies

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(Discontinued) TGX™ FastCast™ Acrylamide Starter Kit, 7.5%

1610170

Starter kit for hand casting 7.5% TGX polyacrylamide gels, includes acrylamide solutions and buffers

(Discontinued) TGX™ FastCast™ Acrylamide Kit, 7.5%

1610171

Kit for hand casting 7.5% TGX polyacrylamide gels, includes acrylamide solutions and buffers

(Discontinued) TGX™ FastCast™ Acrylamide Starter Kit, 10%

1610172

Starter kit for hand casting 10% TGX polyacrylamide gels, includes acrylamide solutions and buffers

(Discontinued) TGX™ FastCast™ Acrylamide Kit, 10%

1610173

Kit for hand casting 10% TGX polyacrylamide gels, includes acrylamide solutions and buffers

(Discontinued) TGX™ FastCast™ Acrylamide Starter Kit, 12%

1610174

Starter kit for hand casting 12% TGX polyacrylamide gels, includes acrylamide solutions and buffers

(Discontinued) TGX™ FastCast™ Acrylamide Kit, 12%

1610175

Kit for hand casting 12% TGX polyacrylamide gels, includes acrylamide solutions and buffers

Accessories

(Discontinued) Mini-PROTEAN® Tetra Vertical Electrophoresis Cell, 4-gel, for 1.0 mm thick handcast gels

1658001FC

4-gel vertical electrophoresis system, 1.0 mm gel thickness, includes companion module, 2 casting stands, 4 casting frames, 10-well combs, 5 short plates, and 5 spacer plates, with TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10% (161-0182)

(Discontinued) Mini-PROTEAN® Tetra Vertical Electrophoresis Cell, 4-gel, for 1.5 mm thick handcast gels

1658006FC

4-gel vertical electrophoresis system, 1.5 mm gel thickness, includes companion module, 2 casting stands, 4 casting frames, 10-well combs, 5 short plates, and 5 spacer plates, with TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10% (161-0182)

Kits & Reagents

(Discontinued) Ammonium Persulfate (APS)

1610700

10 g, ammonium persulfate (APS) catalyst

(Discontinued) 2x Laemmli Sample Buffer

1610737

30 ml, premixed protein sample buffer for SDS-PAGE

(Discontinued) 4x Laemmli Sample Buffer

1610747

10 ml, premixed 4x Laemmli protein sample buffer for SDS-PAGE

(Discontinued) 10x Tris/Glycine/SDS

1610772

Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water

(Discontinued) TEMED

1610800

5 ml, for catalyst for polyacrylamide gels

(Discontinued) TEMED

1610801

50 ml, catalyst for polyacrylamide gels; hazardous material shipping charges may apply

Standards

(Discontinued) Precision Plus Protein™ Dual Color Standards, 2.5 ml

1610394

2.5 ml (5 x 500 µl), mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 250 applications

(Discontinued) Precision Plus Protein™ Kaleidoscope™ Prestained Protein Standards, value pack

1610395

2.5 ml (5 x 500 µl), mixture of ten multicolor recombinant proteins (10–250 kD), 250 applications

(Discontinued) Precision Plus Protein™ Dual Xtra Prestained Protein Standards, value pack

1610397

2.5 ml (5 x 500 µl), mixture of 12 recombinant proteins (2–250 kD), nine blue-stained bands, and three pink reference bands (2, 25, 75 kD), 250 applications

(Discontinued) Precision Plus Protein™ Unstained Protein Standards, Strep-tagged, recombinant, 5 ml

1610396

5 ml (5 x 1 ml), unstained mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 500 applications

Plates

(Discontinued) Mini-PROTEAN^® Short Plates

1653308

Pkg of 5, short plate for Mini-PROTEAN Tetra System and Mini-PROTEAN 3 Multi-Casting Chamber

(Discontinued) Mini-PROTEAN^® Spacer Plates with 0.75 mm Integrated Spacers

1653310

Pkg of 5, spacer plate with 0.75 mm integrated spacer, for Mini-PROTEAN Tetra System and Mini-PROTEAN 3 Multi-Casting Chamber

(Discontinued) Mini-PROTEAN^® Spacer Plates with 1.0 mm Integrated Spacers

1653311

Pkg of 5, spacer plate with 1.0 mm integrated spacer, for Mini-PROTEAN Tetra System and Mini-PROTEAN 3 Multi-Casting Chamber

(Discontinued) Mini-PROTEAN^® Spacer Plates with 1.5 mm Integrated Spacers

1653312

Pkg of 5, spacer plate with 1.5 mm integrated spacer, for Mini-PROTEAN Tetra System and Mini-PROTEAN 3 Multi-Casting Chamber

(Discontinued) Mini-PROTEAN^® Empty Cassettes

4560003

Pkg of 50, cassettes with 10 well marks for casting gels 8.6 x 7.2 cm by 1.0 mm thick, for use with Mini-PROTEAN Electrophoresis Cells; purchase combs separately (456-0013)

(Discontinued) Mini-PROTEAN^® Empty Cassettes

4560005

Pkg of 50, cassettes with 12 well marks for casting gels 8.6 x 7.2 cm by 1.0 mm thick, for use with Mini-PROTEAN Electrophoresis Cells; purchase combs separately (456-0015)

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