Supported Nitrocellulose Membrane, 0.45 µm

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Supported Nitrocellulose transfer membranes, 0.45 µm

Overview

Bio-Rad Protein Transfer Membrane Offerings
Protein Membranes

Bio-Rad's PVDF membranes are available in rolls, precut sheets, or convenient membrane/filter paper sandwiches.

Immun-Blot® PVDF Membrane
  • Ideal for chemiluminescent and colorimetric western blots
  • Retains target protein very strongly during protein transfer but also reduces nonspecific protein binding
Immun-Blot® Low Fluorescence PVDF Membrane
  • Same benefits as standard PVDF membranes plus minimal autofluorescence and high sensitivity
  • Best choice for multiplex fluorescent immunoblotting
Sequi-Blot™ PVDF Membrane
  • Ideal for protein sequencing: retains target protein very strongly during protein transfer
  • Provides the binding capacity to sequence even low-abundance samples
Nitrocellulose Membrane, 0.45 µm
  • Proven medium for western, northern, and Southern blotting
  • 45 µm pore size is recommended for most analytical blotting, including protein, ssDNA, and RNA transfers
  • 0.2 µm pore size is optimized for transferring nucleic acids and low molecular–weight proteins
Nitrocellulose Membrane, 0.2 µm
Supported Nitrocellulose Membrane, 0.45 µm
  • Dense 100% nitrocellulose cast on both sides of an inert polyester support
  • Easy to handle, wets readily, and does not have the brittleness often associated with unsupported nitrocellulose
Supported Nitrocellulose Membrane, 0.2 µm
Supported Nitrocellulose Membranes

Ideal for:

  • Protein blotting
  • DNA/RNA blotting
  • Dot/slot blot screening

 

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Bio-Rad's 0.45 µm pore-size supported nitrocellulose membrane is dense 100% nitrocellulose cast on both sides of an inert polyester support. It is easy to handle, wets readily, and does not have the brittleness often associated with unsupported nitrocellulose.

This supported 0.45 µm nitrocellulose has an excellent signal-to-noise ratio and is recommended for analytical blotting, including most protein, DNA, and RNA transfers. For low molecular weight proteins and nucleic acids, use our 0.2 µm supported nitrocellulose membrane.

Features and Benefits

  • Compatible with all standard immunoblotting and nucleic acid detection methods
  • Provides superior binding capacity without background interference
  • Pore size produces excellent signal-to-noise blotting results
  • Available in in packs of precut membrane or an economical roll

Applications

  • Transfer of protein from SDS-PAGE gels to membrane in preparation for western blotting
  • Transfer of a wide range of protein molecular weights and nucleic acids >500 bp
  • Transfer of nucleic acids in preparation for Northern or Southern blotting
  • Immunodetection of target proteins and probe hybridization of nucleic acids

See the Blotting Membrane and Filter Paper Selection Guide for more information on the different membrane types and size selections by gel type.

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Supported Nitrocellulose Membrane

1620094
Pkg of 1 roll, 0.45 μm, 30 cm x 3 m, bulk blotting membrane for proteins and nucleic acids

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(Discontinued) Supported Nitrocellulose Membranes

1620093
Pkg of 10, 0.45 μm, 20 x 20 cm, precut blotting membrane for proteins and nucleic acids

This product is no longer available. Please contact technical support for more information on replacement product.

The following replacement is recommended:

  • Supported Nitrocellulose Membrane (1620094)
Supported Nitrocellulose Membranes

1620090
Pkg of 10, 0.45 μm, 7 x 8.4 cm, precut blotting membrane for proteins and nucleic acids

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • Supported Nitrocellulose Membrane (1620094)
Supported Nitrocellulose Membranes

1620070
Pkg of 10, 0.45 μm, 8.5 x 13.5 cm, precut blotting membrane for proteins and nucleic acids

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • Supported Nitrocellulose Membrane (1620094)
(Discontinued) Supported Nitrocellulose Membranes

1620254
Pkg of 10, 0.45 μm, 26.5 x 28 cm, precut blotting membrane for proteins and nucleic acids

This product is no longer available. Please contact technical support for more information on replacement product.
Number Description Options
2895 Protein Blotting Guide, Ver C Click to download

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