SsoAdvanced Universal SYBR Green Supermix

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Overview

Optimized for high processivity and robust performance with demanding templates. Use with any real-time PCR instrument over a wide range of reaction and cycling conditions.
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Description

Description

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SsoAdvanced Universal SYBR Green Supermix is an exclusive high-performance real-time PCR reagent based on Bio-Rad's patented* Sso7d fusion protein polymerase technology and advanced buffer formulation.

This supermix is uniquely formulated to provide higher processivity, increased PCR inhibitor tolerance, and robust performance with challenging templates and targets in SYBR Green-based qPCR. It delivers superior performance for a wide range of applications on any qPCR platform and is validated to ensure optimal results with Bio-Rad’s PrimePCR Gene Expression Assays.

SsoAdvanced Universal SYBR Green Supermix allows you to:

  • Achieve superior real-time PCR results under various experimental conditions — for example, primer concentrations, annealing temperatures, salt concentrations, and/or cycling protocol (standard or fast)
  • Increase your qPCR sensitivity and efficiency of detection from compromised samples — the highly processive Sso7d fusion polymerase has increased resistance to various PCR inhibitors, enhancing sensitivity and overall performance
  • Shorten time to results while maintaining qPCR data quality — the Sso7d fusion polymerase and optimized buffer provide rapid polymerization kinetics and instant polymerase activation
  • Use any real-time PCR system — the universal reference dye in this supermix enables ROX normalization of qPCR data regardless of the ROX level requirements of the qPCR system

Instrument Compatibility

SsoAdvanced Universal SYBR Green Supermix is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:

Systems
Applied Biosystems
  • StepOne/StepOnePlus
  • 7500, ViiA 7
  • 7000, 7300, 7700, 7900HT, QuantStudio Systems
Stratagene
  • Mx3000P, 3005P, 4000
Eppendorf
  • Mastercycler ep realplex 2, 4
QIAGEN
  • Corbett Rotor-Gene 3000, 6000, Q
Roche
  • LightCycler 480, 96
  • LightCycler 1.0, 1.5, 2.0*
BioFire
  • LightScanner HR-1
  • LightScanner 32*

* BSA must be added according to instrument specifications

Applications and Uses of SsoAdvanced Universal SYBR Green Supermix

Related Products

Bio-Rad offers complete workflow solutions for real-time PCR:

More Information

To ensure that your qPCR reactions are reproducible and reliable, follow our step-by-step design and optimization protocols. We also provide insights into analysis of qPCR data and troubleshooting reactions.

* U.S. patent 7,560,260

Specifications

Specifications

Product expiration (after shipping date)
12 months
Storage at 4°C (after thawing)
Up to 3 months
Customer guarantee (minimum usage time) at –20°C
12 months

Ordering

Ordering

items

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1725270
2 ml (2 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX normalization dyes, for 200 x 20 µl reactions
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1725271
5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX Normalization Dyes, for 500 x 20 µl reactions
ssoadvanced-universal-sybr-green-supermix-172-5272-thumb.jpg
1725272
10 ml (10 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX normalization dyes, for 1,000 x 20 µl reactions
ssoadvanced-universal-sybr-green-supermix-172-5274-thumb.jpg
1725274
25 ml (5 x 5 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX normalization dyes, for 2,500 x 20 µl reactions
ssoadvanced-universal-sybr-green-supermix-172-5275-thumb.jpg
1725275
50 ml (10 x 5 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX normalization dyes, for 5,000 x 20 µl reactions

Documents

Documents

Number Description Options
10000076382
SsoAdvanced™ Universal SYBR® Green Supermix Product Insert
10031339
Instruction Manual, SsoAdvanced™ Universal SYBR® Green Supermix, Rev A
6252
Reagent Comparison Guide for Real-Time PCR Brochure, Rev C
[ Add to Cart (Free) ]
7178
Amplification: Real-Time PCR Reagents and Kits Brochure
6090
Amplification Reagents and Plastics Brochure
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6763
Gene Expression Real-Time PCR Workflow Flier
[ Add to Cart (Free) ]
6629
High-Throughput Real-Time PCR Solutions Brochure