PureZOL™ RNA Isolation Reagent

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Overview

The ready-to-use PureZOL RNA isolation reagent is an efficient means of isolating RNA from a variety of sources, including cultured cell, animal and plant tissue, yeast, virus, and bacteria.
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Description

Description

The ready-to-use PureZOL RNA isolation reagent is a versatile and efficient means of isolating high yields of RNA from a variety of sources, including cultured cell, animal and plant tissue, yeast, virus, and bacteria samples. The protocol is an improvement over the rapid, widely used, and proven method of RNA isolation developed by Chomczynski and Sacchi (1987).

PureZOL RNA isolation reagent is a potent monophasic combination of phenol and the chaotropic agent guanidine isothiocyanate, which effectively lyses cells and tissues, deproteinates RNA, and inactivates endogenous nucleases in a single step. The single-solution format permits recovery of RNA from small quantities of tissues or cells, making it ideally suited for gene expression studies or whenever limited sample quantities are available.

Protocols are scalable to accommodate a wide range of sample sizes. DNA and protein that are efficiently removed from the RNA, following phase separation, can be subsequently extracted from the same sample origin.

Features include:

  • Effective total RNA isolation from a wide variety of samples in less than 60 minutes
  • Scalable starting sample amount
  • Convenient isolation of RNA, DNA, and protein from the same sample source

Reference

Chomczynski P and Sacchi N (1987). Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162, 156-159.

Specifications

Specifications

Format
Single-solution
Method
Organic extraction
Number of preps
50 or 100 (1ml/prep)
Maximum starting material amounts
100 mg animal or plant tissues
1 x 107 cultured cells
2.4 x 109 bacterial cells
3 x 107 yeast cells
Purity
A260/A280 of 1.9–2.1
Preparation time
<60 min

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Kits & Reagents

Kits & Reagents

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7326830
DNA-free RNA isolation kit, includes PureZOL RNA isolation reagent, RNA binding columns, all collection and sample tubes, lyophilized DNase I, RNase-free reagents and plasticware, for 50 preps
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7326870
DNA-free RNA isolation kit, includes columns and tubes, DNase I, RNase-free reagents (PureZOL RNA isolation reagent sold separately), for 50 preps

Documents

Documents

Number Description Options
6090
Amplification Reagents and Plastics Brochure
[ Add to Cart (Free) ]
6763
Gene Expression Real-Time PCR Workflow Flier
[ Add to Cart (Free) ]
10001481
Instruction Manual, PureZOL RNA Isolation Reagent, Rev A