CHEF-DR III Variable Angle System

Image
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Overview

The CHEF-DR III variable angle system combines patented PACE technology and proven CHEF technology into one easy-to-use instrument.
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Description

Description

The CHEF-DR® III variable angle system combines patented PACE technology and proven CHEF technology into one easy-to-use instrument that lets you use up to three blocks of run conditions to generate high-resolution separations; specify any electrophoresis angle from 90 to 120°. Separation of chromosome-sized DNA can be achieved in almost half the time previously possible, without sacrificing resolution.

Each CHEF-DR III system is supplied with a power module, electrophoresis cell, variable-speed pump, and accessory kit.The CHEF-DR III chiller systems also come with a cooling module.

Automation
The CHEF-DR III system has built-in automation. It will recall the last conditions and use them as the default protocol. The CHEF-DR III system will also recall current run conditions and run progress if interrupted by power failure and resume the run without user intervention.

Customization
With the CHEF-DR III system, conditions are entered by the user. The instruction manual describes extensive examples of run conditions based on a variety of size separation ranges.

Application Versatility
The ability to program the angle of electrophoresis with the CHEF-DR III system enables fast, high-resolution separations of DNA molecules ranging from 200 kb to >6 Mb. The CHEF-DR III system allows selection of the optimal voltage gradient, switch time, and angle for the specific DNA size range. Separations can be further optimized by programming up to three consecutively executing blocks of run conditions, increasing the variety of possible run conditions.

Specifications

Specifications

Power Module
 
Dimensions (W x D x H)
48 x 43 x 17.5 cm
Construction
Aluminum chassis
Weight
10 kg (22 lb)
Power supply
350 V maximum, to allow maximum gradient of 9 V/cm, continuously adjustable; built in
Electrical
 
Maximum amps
0.5 A
Allowable voltage gradients
0.6–9 V/cm, in 0.1 V/cm increments
Battery back-up
All parameters in memory
Electrode potentials
Dynamically regulated (feedback adjustment) +/- 0.5%
Data entry
Keyboard
Functional
 
Switching range
0.1 sec–65,000 sec
Switch angle variable
90–120° (all electronic switching) in 1° increments
Maximum program blocks
3, with automatic execution
Maximum run time
999 hr per block
Input voltage range
100–120 VAC/50–60 Hz/4 A
220–240 VAC/50–60 Hz/2 A
Fuses
0.5 A Fast Blow for high voltage output
3.15 A Slow-Blow (100/120 V) or
1.60 A Slow-Blow (220/240 V)
Environmental
 
Operating temperature
50°F (10°C) to 90°F (32°C); 30–80% humidity
Storage temperature
32°F (0°C) to 140°F (60°C); 10–90% humidity
Electrophoresis Cell
 
Dimensions (W x D x H)
44.2 x 50.3 x 11.4 cm, horizontal format
Construction
Cover: vacuum-formed polycarbonate; base: injection-molded polycarbonate
Lid
Safety interlocked
Weight
10.2 kg (22.5 lb)
Electrodes
24, platinum (0.02" diameter)
Temperature monitoring
Via precision temperature probe mounted in base of cell
Accessories Included
 
Variable speed pump
120 V, ground isolated; flow rate 1 l/min, typical
Casting stand
14 cm x 13 cm
Comb
10-well comb and comb holder
Tygon tubing
365 cm
Disposable sample plug mold
50 slot
Yeast DNA standard
S. cerevisiae YNN295, 2 plugs
Chromosomal grade agarose
5 g
Pulsed field Certified agarose
5 g
Leveling bubble
1
Fuses
0.5 A Fast Blow, 2 spares
Manual
1
Screened cap
1
Cooling Module (optional)
 
Weight
14 kg (30.9 lb)
Construction
Aluminum
Dimensions (W x L x H)
23 x 42 x 24 cm
Cooling capacity
75 W of input power at 14°C
Operating range
5–25°C
Total system weight
41.7 kg (91.9 lb)

Ordering

Ordering

items

Use the filters below to refine results!

170-3700_tn.jpg
1703700
120 V, complete variable angle PFGE system with cooling module, includes all gel casting accessories, pump, tubing, DNA standards, 2 plugs of S. cerevisiae DNA size standard, PFGE grade agarose
170-3700_tn.jpg
1703702
220/240 V, complete variable angle PFGE system with cooling module, includes all gel casting accessories, pump, tubing, DNA standards, 2 plugs of S. cerevisiae DNA size standard, PFGE grade agarose
170-3703_tn.jpg
1703703
100 V, complete variable angle PFGE system with cooling module includes all gel casting accessories, pump, tubing, DNA standards, 2 plugs of S. cerevisiae DNA size standard, PFGE grade agarose

Accessories

Accessories

1703649
Horizontal electrophoresis cell for use with with CHEF Mapper XA, CHEF DR-II, and CHEF DR-III Variable Angle systems
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1703654
pulsed field gel electrophoresis cooling system, for use with all 120 V CHEF chiller systems
170-3688_tn.jpg
1703688
pulsed field gel electrophoresis cooling system, for use with all 100 V CHEF chiller systems
170-3655_tn.jpg
1703655
pulsed field gel electrophoresis cooling system, for use with all 220/240 V CHEF chiller systems
170-3644_tn.jpg
1703644
120 V, pulsed field gel electrophoresis pump, for use with any CHEF system
170-3648_tn.jpg
(Discontinued) CHEF Electrodes
1703648
Pkg of 6, thick gauge (0.02"), pulsed field gel electrophoresis electrodes, for use with any CHEF system
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(Discontinued) CHEF Screened Caps
1703711
Pkg of 5, pulsed field gel electrophoresis sample preparation accessory for use with any CHEF system
170-3713_tn.jpg
1703713
Pkg of 5x50 pulsed field gel electrophoresis disposable plug molds, for use with any CHEF system
170-3622_tn.jpg
1703622
Pkg of 1, 10 plug mold for PFGE, for use with any CHEF system
170-3689_tn.jpg
1703689
Pkg of 1, pulsed field gel electrophoresis casting stand, includes 14 x 13 cm frame and platform, for use with any CHEF system
170-3704_tn.jpg
1703704
Pkg of 1, pulsed field gel electrophoresis casting stand, includes 21 x 14 cm frame and platform, for use with any CHEF system
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(Discontinued) CHEF Leveling Table
1704046
Pkg of 1, gel-casting level surface, 20 x 30 cm, for use with the CHEF Mapper XA chiller system
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(Discontinued) Gel Scoop
1703643
Pkg of 1, pulsed field gel electrophoresis gel scoop, for use with any CHEF system

Kits & Reagents

Kits & Reagents

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1613108
25 g, agarose for use with CHEF and FIGE applications, certified free of inhibitors, DNases, RNases
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1613109
125 g, agarose for use with CHEF and FIGE applications, certified free of inhibitors, DNases, RNases
161-3110_tn.jpg
1613110
500 g, agarose for use with CHEF and FIGE applications, certified free of inhibitors, DNases, RNases
161-3100_tn.jpg
1613100
25 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases
161-3101_tn.jpg
1613101
125 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases
161-3102_tn.jpg
1613102
500 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases
162-0137_tn.jpg
1620137
100 g, agarose for separation of large DNA in pulsed field gel applications, certified free of inhibitors, DNases, RNases
162-0138_tn.jpg
1620138
500 g, agarose for separation of large DNA in pulsed field gel applications, certified free of inhibitors, DNases, RNases
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1610733
1 L, TBE nucleic acid electrophoresis buffer solution, pH 8.3
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1610770
5 L cube, TBE nucleic acid electrophoresis buffer solution, pH 8.3
161-0743_tn.jpg
1610743
1 L, TAE nucleic acid electrophoresis buffer solution, pH 8.0
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1610773

5 L cube, TAE nucleic acid electrophoresis buffer solution, pH 8.0

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1610433
10 mL ethidium bromide solution for fluorescent staining of nucleic acids, 10 mg/mL
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(Discontinued) EDTA
1610729
500 g, ≥99% pure EDTA (disodium ethylenediaminetetraacetate dihydrate) powder
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(Discontinued) Tris
1610716
500 g, ≥99.8% pure Tris base (Tris[hydroxymethyl]aminomethane or Trimethamine)
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(Discontinued) Tris
1610719
1 kg, ≥99.8% pure Tris base (Tris[hydroxymethyl]aminomethane or Trimethamine)

Combs

Combs

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1704326
Adjustable-height comb, for use with Sub-Cell and Wide Mini-Sub Cell GT Systems (requires comb holder #1704320) or CHEF-DR and CHEF Mapper Systems (requires comb holder #1703699)
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1704325
Adjustable-height comb, for use with Sub-Cell and Wide Mini-Sub Cell GT Systems (requires comb holder #1704320) or CHEF-DR and CHEF Mapper Systems (requires comb holder #1703699)
170-4324_tn.jpg
1704324
Adjustable-height comb, for use with Sub-Cell and Wide Mini-Sub Cell GT Systems (requires comb holder #1704320) or CHEF-DR and CHEF Mapper Systems (requires comb holder #1703699)
170-4323_tn.jpg
1704323
Adjustable-height comb, for use with Sub-Cell and Wide Mini-Sub Cell GT Systems (requires comb holder #1704320) or CHEF-DR and CHEF Mapper Systems (requires comb holder #1703699)
170-4322_tn.jpg
1704322
Adjustable-height comb, for use with Sub-Cell and Wide Mini-Sub Cell GT Systems (requires comb holder #1704320) or CHEF-DR and CHEF Mapper Systems (requires comb holder #1703699)
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1704344
Adjustable-height comb, for use with Sub-Cell and Wide Mini-Sub Cell GT Systems (requires comb holder #1704320) or CHEF-DR and CHEF Mapper Systems (requires comb holder #1703699)
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1703627
Pkg of 1, 15-well pulsed field gel electrophoresis comb, for use with CHEF-DR® and CHEF Mapper® Systems (requires comb holder #1703699) or any CHEF system
170-3628_tn.jpg
1703628
Pkg of 1, 30-well pulsed field gel electrophoresis comb, for use with CHEF-DR® and CHEF Mapper® Systems (requires comb holder #1703699) or any CHEF system
170-3645_tn.jpg
1703645
Pkg of 1, 45-well pulsed field gel electrophoresis comb, for use with CHEF-DR® and CHEF Mapper® Systems (requires comb holder #1703699) or any CHEF system
170-3623_tn.jpg
1703623
Pkg of 1, preparative comb with 2 outer wells for size standards, for use with CHEF-DR® and CHEF Mapper® Systems (requires comb holder #1703699) or any CHEF system
170-3699_tn.jpg
1703699
Pkg of 1, pulsed field gel electrophoresis comb holder, for use with any CHEF system

Standards

Standards

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1703624
200 μl, 0.1 μg/μl, PFGE standard, 5 kb ladder, 4.9–120 kb, concatemers of pBR328, 20–25 applications
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1703707
125 μl, 0.2 μg/μl, PFGE standard, 8.3–48.5 kb, mixed digests of phage lambda (λ), 125 applications
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1703635
5 agarose blocks, PFGE standard, lambda (λ) ladder, 48.5-1000 kb in 48.5 kb increments, concatemers of λcl857Sam7 lambda DNA per block, for 25–40 plugs
170-3605_tn.jpg
1703605
5 agarose blocks, PFGE marker, 0.225–2.2 Mb S. cerevisiae chromosomal DNA, for 25–40 plugs
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1703667
5 agarose blocks, PFGE marker, 1–3.1 Mb, H. wingei chromosomal DNA, for 25–40 plugs
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(Discontinued) CHEF DNA Size Marker
1703633
5 agarose blocks, PFGE marker, 3.5–5.7 Mb, S. pombe chromosomal DNA, for 25–40 plugs

Documents

Documents

Number Description Options
1589
Preparation and Separation of Mammalian DNA by Pulsed Field Gel Electrophoresis Bulletin, Rev A
LIT526
Instruction Manual, Standard Casting Stand Combination Comb Holder Wide/Long Combination Casting Stand
9204213
Instruction Manual, CHEF Variable Speed Pump, Rev B
1624
Model 1000 Mini Chiller Product Information Sheet
M1703690
Instruction Manual, CHEF-DR III Pulsed Field Electrophoresis Systems, Rev B
5901
Pulsed Field Gel Electrophoresis Brochure, Rev B
6228
Using Pulsed Field Gel Electrophoresis Standards, Rev A