Mini-PROTEAN TGX Precast Gels for 2-D Electrophoresis

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Overview

Mini-PROTEAN TGX precast gels are long shelf life gels that use standard Tris/glycine buffers. The gels are ideal for mini-format 2-D electrophoresis because they deliver superior resolution and fast run times.
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Description

Description

The long shelf life Mini-PROTEAN® TGX™ precast gels are ideal for mini-format 2-D electrophoresis because they are:

  • robust in handling different sample types and sample solution conditions
  • versatile in molecular weight range, especially the Any kD™ gel with range from 10–250 kD
  • capable of separations of complex samples with several hundred identifiable protein spots

The Mini-PROTEAN TGX precast gels are an innovative PAGE system designed to provide Laemmli-like separation patterns using a standard Tris/glycine running buffer system. The Mini-PROTEAN gels are compatible with Mini-PROTEAN Tetra (1–4 gels) and Dodeca™ (1–12 gels) cells. These gels can also be used in the discontinued Mini-PROTEAN 3 cell.

Benefits of Mini-PROTEAN TGX precast gels include:

  • Direct drop-in for Laemmli systems
  • Inexpensive buffer system, low running costs
  • Fast run times
  • Accurate MW estimation
  • Bottom-open cassette design for easy gel handling and faster blotting setup
  • Easy-to-open cassette for faster downstream processing

Specifications

Specifications

Gel dimensions (W x L x thickness)
8.6 x 6.7 x 0.1 cm
Cassette dimensions (W x L x thickness)
10 x 8 x 0.46 cm
Cassette material
Styrene copolymer
Comb material
Polycarbonate
Gel storage conditions
Store flat at 2–8°C; do not freeze
Shelf life at recommended temperature*
12 months
Recommended sample buffer (Laemmli, dilute 1:1 with sample)
62.5 mM Tris-HCl, pH 6.8, 2% SDS, 25% glycerol, 0.01% bromophenol blue
Recommended running buffer (Tris/glycine/SDS)
25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3

* From date of manufacture

 

Ordering

Ordering

items

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4569031
Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4561031
Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4561041
Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4561081
Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4561091
Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4561021
Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

Kits & Reagents

Kits & Reagents

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1610738
30 ml, premixed protein sample buffer for native PAGE, contains 62.5 mM Tris-HCl, pH 6.8, 40% glycerol, 0.01% bromophenol blue
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(Discontinued) 10x Tris/Glycine/SDS
1610732
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water
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1610734
Pkg of 1, 1 L, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water
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1610363
1 ml, unstained mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 100 applications
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1610393
2.5 ml (5 x 500 µl), mixture of ten blue-stained recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 250 applications
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1610374
500 μl, mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 50 applications
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1610375
500 µl, mixture of ten multicolor recombinant proteins (10–250 kD), 50 applications
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1610376
250 μl, pre-stained, Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, 75 kD), 50 applications
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1610376
250 μl, pre-stained, Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, 75 kD), 50 applications

Documents

Documents

Number Description Options
1658100
Mini-PROTEAN Precast Gels Instruction Manual and Application Guide
5871
Mini-PROTEAN® TGX™ Precast Gels Product Information Sheet, Rev F
[ Add to Cart (Free) ]
5910
Mini-PROTEAN TGX Precast Gel: A Gel for SDS-PAGE with Improved Stability — Comparison with Standard Laemmli Gels, Rev B
5911
Mini-PROTEAN TGX Precast Gel: A Versatile and Robust Laemmli-Like Precast Gel for SDS-PAGE, Rev A.
5932
Ready Gel to Mini-PROTEAN TGX Precast Gels Catalog Number Conversion Chart, Rev E
5934
NuPAGE Bis-Tris Precast Gels (MOPS Buffer) to Mini-PROTEAN TGX Precast Gels Catalog Number Conversion Chart, Rev G