
Overview
Cronobacter, formerly known as Enterobacter sakazakii, is a ubiquitous pathogen that has been isolated from food, environmental and clinical samples. It is associated with rare but fatal infant infections linked to the consumption of reconstituted powdered infant formula.
The iQ-Check Cronobacter spp. PCR Detection Kit is based on the fast, sensitive, and proven technology of real-time PCR. The kit is intended for the detection of Cronobacter spp. in food, mainly infant formula and baby food, and environmental samples.
Due to the sensitivity and specificity of PCR, results can be obtained in as little as 16 hr following a single enrichment in nonselective buffered peptone water (3564684 and 3554179).
Highly Specific and Reliable
The iQ-Check Cronobacter spp. Kit uses an optimized system of primers and probes to ensure high specificity. It is designed as a multiplex reaction that includes an internal inhibition control that is amplified in parallel with the target DNA for a reliable result.
Maximum Flexibility
Developed as an open and flexible system, the iQ-Check Cronobacter spp. Kit can be used for up to 94 samples. Reactions can be run on low- or high-throughput Bio-Rad instruments, depending on the laboratory’s needs. Different iQ-Check assays can be run in parallel on the same plate.
A Complete Solution
Bio-Rad offers a complete solution with standardized reagents, such as enrichment media, PCR kits, confirmation agars, and PCR detection systems, in addition to software for automated analysis specific to each test.
Kit Components
- Lysis reagent for DNA extraction
- Fluorescent probes
- PCR amplification mix
- Positive and negative PCR controls
Ordering
items
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Pkg of 1, 1 vial of lyophilized iQ-Check Free DNA Removal Reagent (to make 10 ml) and 1 vial of iQ-Check 10x Activation Buffer (9 ml)

Pkg of 1, vial of Purification Reagent (to make 25 ml), for 96 tests

Pkg of 1, 2 g vial

Pkg of 6, 225 ml bottles — for challenging and non-challenging enrichment conditions



Pkg of 1, 500 g — for challenging and non-challenging enrichment conditions




Accessories









Pkg of 100, optically clear seal for PCR plates for real-time PCR

Pkg of 500, screwcap micro test tube, sterilized, with installed O-ring screwcaps

Pkg of 960, 20–200 µl pipet tip with aerosol barrier, racked, certified free of pyrogen, DNase, RNase; For food application only

Pkg of 1,000, 100–1,000 µl pipet tip with aerosol barrier, bulk, certified free of pyrogen, DNas

Pkg of 960, 100–1,000 µl pipet tip with aerosol barrier, racked, certified free of pyrogen, DNase, RNase, For food application only

Pkg of 960, 2–20 µl pipet tip with aerosol barrier, racked, certified free of pyrogen, DNase, RNase; For food application only
Equipment

Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, CFX96 Deep Well Optical Reaction Module, cables. To be used with CFX Manager Software, Industrial Diagnostic Edition.

96-well, 5-color plus FRET, network-connected real-time PCR detection system, includes CFX Opus 96 base unit, cables

96-well, 5-color plus FRET, network-connected real-time PCR detection system, includes CFX Opus 96 base unit, and cables

CFX Manager Software, Industrial Diagnostic Edition

